Anti-bacterial methods and materials

ABSTRACT

Staphylococcus aureus  virulence genes are identified, thereby allowing the identification of novel anti-bacterial agents that target these virulence genes and their products, and the provision of novel  S. aureus  mutants useful in vaccines.

[0001] The invention relates generally to the identification of genesresponsible for the virulence of Staphylococcus bacteria, therebyallowing the identification of new anti-bacterial agents that targetthese virulence genes and their products and the provision of novel S.aureus mutants useful in vaccines.

BACKGROUND OF THE INVENTION

[0002] The staphylococci, of which Staphylococcus aureus is the mostimportant human pathogen, are hardy, gram-positive bacteria thatcolonize the skin of most humans. Staphylococcal strains that producecoagulase are designated S. aureus; other clinically importantcoagulase-negative staphylococci are S. epidermidis and S.saprophyticus. When the skin or mucous membrane barriers are disrupted,staphylococci can cause localized and superficial infections that arecommonly harmless and self-limiting. However, when staphylococci invadethe lymphatics and the blood, potentially serious complications mayresult, such as bacteremia, septic shock, and serious metastaticinfections, including endocarditis, arthritis, osteomyelitis, pneumoniaand abscesses in virtually any organ. Certain strains of S. aureusproduce toxins that cause skin rashes, food poisoning, or multisystemdysfunction (as in toxic shock syndrome). S. aureus and S. epidermidistogether have become the most common cause of nosocomial non-urinarytract infection in U.S. hospitals. They are the most frequently isolatedpathogens in both primary and secondary bacteremias and in cutaneous andsurgical wound infections. See generally Harrison's Principles ofInternal Medicine, 13th ed., Isselbacher et al., eds., McGraw-Hill, NewYork (1994), particularly pages 611-617.

[0003] Transient colonization of the nose by S. aureus is seen in 70 to90 percent of people, of which 20 to 30 percent carry the bacteria forrelatively prolonged periods of time. Independent colonization of theperineal area occurs in 5 to 20 percent of people. Higher carriage ratesof S. aureus have been documented in persons with atopic dermatitis,hospital employees, hospitalized patients, patients whose care requiresfrequent puncture of the skin, and intravenous drug abusers.

[0004] Infection by staphylococci usually results from a combination ofbacterial virulence factors and a diminution in host defenses. Importantmicrobial factors include the ability of the staphylococcus to surviveunder harsh conditions, its cell wall constituents, the production ofenzymes and toxins that promote tissue invasion, its capacity to persistintracellularly in certain phagocytes, and its potential to acquireresistance to antimicrobials. Important host factors include an intactmucocutaneous barrier, an adequate number of functional neutrophils, andremoval of foreign bodies or dead tissue.

[0005] Cell wall components of S. aureus include a large peptidoglycancomplex that confers rigidity on the organism and enables it to surviveunder unfavorable osmotic conditions, a unique teichoic acid linked topeptidoglycan, and protein A, which is found both attached topeptidoglycan over the outermost parts of the cell and released insoluble form. Proteins designated femA and femB are involved in theformation of cell wall peptidoglycan pentaglycine cross-bridges and arefactors in methicillin resistance. [Berger-Bachi et al., Mol. Gen.Genet., 219:263-269 (1989).] S. aureus also has specific receptors forlaminin and fibronectin that may mediate the organism's spread throughthe bloodstream to other tissues. Both peptidoglycan and teichoic acidare capable of activating the complement cascade via the alternativepathway. S. aureus also appears to activate tissue factor in thecoagulation pathway.

[0006] Certain enzymes produced by S. aureus may play a role invirulence. Catalase degrades hydrogen peroxide and may protect theorganism during phagocytosis. Coagulase is present in both soluble andcell-bound forms and causes plasma to clot by formation of thrombin-likematerial. The high correlation between coagulase production andvirulence suggests that this substance is important in the pathogenesisof staphylococcal infections, but its precise role as a determinant ofpathogenicity has not been determined. Many strains also producehyaluronidase, an enzyme that degrades hyaluronic acid in the connectivetissue matrix and that may promote spreading of infection. Atrypsin-like protease from some strains enhances influenza virusinfection by proteolytic cleavage of the viral precursor hemagglutinininto its active fragments and may contribute to the morbidity of suchcoinfections.

[0007]S. aureus produces numerous extracellular exotoxins that have beenimplicated in disease processes. The exfoliatin toxins A and B, thestaphylococcal enterotoxins, and the toxic shock syndrome toxin, TSST-1,belong to the growing family of microbial superantigens that activate Tcells and monocytes/macrophages, resulting in the production ofcytokines that mediate local or systemic effects depending on the amountof toxin formed, the immune status of the host, and the access of thetoxin to the circulation. The exfoliatin toxins mediate the dermatologicmanifestations of the staphylococcal scalded-skin syndrome and bullousimpetigo. These toxins cause intraepidermal cleavage of the skin at thestratum granulosum, leading to bullae formation and denudation. Sevendistinct enterotoxins (A, B, C1, C2, C3, D, and E) have been implicatedin food poisoning due to S. aureus. These toxins enhance intestinalperistalsis and appear to induce vomiting by a direct effect on thecentral nervous system. Toxic shock syndrome (TSS) is most commonlymediated by TSST-1, which is present in 5 to 25 percent of clinicalisolates of S. aureus. TSS is also mediated less frequently byenterotoxin B and, rarely, enterotoxin C1.

[0008]S. aureus produces other toxins whose role in virulence isincompletely understood. Four different red blood cell hemolysins, whichare designated alpha, beta, gamma, and delta toxins, have beenidentified. Alpha toxin also causes necrosis of the skin when injectedsubcutaneously into animals, while delta toxin also inhibits waterabsorption in the intestines and may play a role in the acute waterydiarrhea seen in some cases of staphylococcal infection. Leukocidinlyses granulocyte and macrophage membranes by producing membrane porespermeable to cations.

[0009] The agr, xpr, sae and sar genes have been identified as beinginvolved in the regulation of staphylococcal exotoxins. See U.S. Pat.No. 5,587,228 and International Patent Publication Nos. WO 96/10579 andWO 97/11690. Of interest is the report in WO 97/11690 of screening forinhibitors of these regulatory systems.

[0010] Staphylococci can invade the skin or mucosa through plugged hairfollicles and sebaceous glands or areas traumatized by bums, wounds,abrasions, insect bites, or dermatitis. Staphylococci often colonizeprosthetic devices and intravenous catheters; S. aureus infection of thevascular access site is a major cause of morbidity and death amongpatients on hemodialysis. Colonization and invasion of the lungs mayoccur with endotracheal intubation, or when the lungs' clearancemechanisms are depressed, e.g., after viral infections, afteraspiration, or in patients with cystic fibrosis. Mucosal damage to thegastrointestinal tract following cytotoxic chemotherapy or radiotherapypredisposes to invasion from that site.

[0011] Once the skin or mucosa have been breached, local bacterialmultiplication is accompanied by inflammation, neutrophil accumulation,tissue necrosis, thrombosis and fibrin deposition at the site ofinfection. Later, fibroblasts create a relatively avascular wall aboutthe area. When host mechanisms fail to contain the cutaneous orsubmucosal infection, staphylococci may enter the lymphatics and thebloodstream. Common sites of metastatic spread include the lungs,kidneys, cardiac valves, myocardium, liver, spleen, bones and brain.

[0012] Bacteremia due to S. aureus may arise from any local infection,at either extravascular (cutaneous infections, burns, cellulitis,osteomyelitis, arthritis) or intravascular foci (intravenous catheters,dialysis access sites, intravenous drug abuse). Rarely, patients withbacteremia die within 12 to 24 hours with high fever, tachycardia,cyanosis, and vascular collapse. Disseminated intravascular coagulationmay produce a disease mimicking meningococcemia. Commonly, the diseaseprogresses more slowly, with hectic fever and metastatic abscessformation.

[0013] A major complication of S. aureus bacteremia is endocarditis. S.aureus is the second most common cause of endocarditis and the mostcommon cause among drug addicts. The disease is typically acute, withhigh fever, progressive anemia, and frequent embolic and extracardiacseptic complications. Valve ring and myocardial abscesses are common.The mortality rate is 20 to 30 percent.

[0014] Staphylococcal scalded-skin syndrome (SSSS) is a generalizedexfoliative dermatitis that is a complication of infection by exfoliatintoxin-producing strains of S. aureus. The disease typically occurs innewborns (Ritter's disease) and in children under the age of five. Ascarlatiniform rash begins in the perioral area, becomes generalizedover the trunk and extremities, and finally desquamates. The disease mayconsist of rash alone (staphylococcal scarlet fever), or large, flaccidbullae develop that may be localized (more common in adults) orgeneralized. The bullae burst, resulting in red, denuded skin resemblinga burn. Most adults with SSSS are immunosuppressed or have renalinsufficiency. Blood cultures are frequently positive, and mortality issignificant.

[0015] Toxic shock syndrome (TSS) is a multisystem disease mediated bytoxins (generally TSST-1, and less frequently enterotoxins B and Cl)produced by certain strains of S. aureus. It was first described inchildren, but in 1980 became epidemic among young women, with onsetduring menstruation. The diagnosis of TSS is based on clinical criteriathat include high fever, a diffuse rash that desquamates on the palmsand soles over the subsequent one or two weeks, hypotension that may beorthostatic, and evidence of involvement in three or more organ systems.Such involvement commonly includes gastrointestinal dysfunction(vomiting or diarrhea), renal or hepatic insufficiency, mucous membranehyperemia, thrombocytopenia, myalgias with elevated creatinephosphokinase (CK) levels, and disorientation with a normalcerebrospinal fluid examination. The mortality rate of TSS is threepercent.

[0016]S. aureus causes approximately three percent of community-acquiredbacterial pneumonias. This disease occurs sporadically except duringinfluenza outbreaks, when staphylococcal pneumonia is relatively morecommon, although still less frequent than pneumococcal pneumonia.Primary staphylococcal pneumonia in infants and children frequentlypresents with high fever and cough. Multiple thin-walled abscesses areseen on the chest X-ray, and empyema formation is common. In olderchildren and healthy adults, staphylococal pneumonia is generallypreceded by an influenza-like respiratory infection. Onset ofstaphylococcal involvement is abrupt, with chills, high fever,progressive dyspnea, cyanosis, cough, pleural pain, and sometimes bloodysputum. Staphylococcal pneumonia is seen more frequently in patientswith cystic fibrosis, in intubated patients in intensive care units andin debilitated patients who are prone to aspiration.

[0017]S. aureus is responsible for the majority of cases of acuteosteomyelitis. Although the disease is most common in people under theage of 20, it is becoming increasingly prevalent in adults over 50,particularly with involvement of the spine. A primary portal of entry isfrequently not identified, although many patients give a history ofpreceding trauma to the involved area. Once established, infectionspreads through the bone to the periosteum or along the marrow cavity.Rarely, the joint capsule is penetrated, producing pyogenic arthritis.Osteomyelitis in children may present as an acute process beginningabruptly with chills, high fever, nausea, vomiting, and progressive painat the site of bony involvement.

[0018]S. aureus causes 1 to 9 percent of cases of bacterial meningitisand 10 to 15 percent of brain abscesses. Most commonly, the bacteria arespread from a focus outside the central nervous system, typically frominfective endocarditis, by extension from a paraspinal or parameningealabscess, or by nosocomial infection following neurosurgical procedures.Over 50 percent of epidural abscesses are due to S. aureus; up to halfof these cases may be associated with vertebral osteomyelitis. Patientspresent with either acute or chronic back pain, usually with low-gradefever and malaise. The onset of radicular pain is an ominous sign thatthe disease may progress to neurologic dysfunction and ultimateparalysis.

[0019] Antimicrobial resistance by staphylococci favors theirpersistence in the hospital environment. Over 90 percent of bothhospital and community strains of S. aureus causing infection areresistant to penicillin. This resistance is due to the production ofβ-lactamases enzymes; the genes for these enzymes are usually carried byplasmids. Infections due to organisms with such acquired resistance cansometimes be treated with penicillinase-resistant β-lactam antimicrobialagents. However, the true penicillinase-resistant S. aureus organisms,called methicillin-resistant S. aureus (MRSA), are resistant to all theβ-lactam antimicrobials as well as the cephalosporins. MRSA resistanceis chromosomally mediated and involves production of an alteredpenicillin-binding protein (PBP 2a or PBP 2′) with a low bindingaffinity for β-lactams. MRSA frequently also have acquired plasmidsmediating resistance to erythromycin, tetracycline, chloramphenicol,clindamycin, and aminoglycosides. MRSA have become increasingly commonworldwide, particularly in tertiary-care referral hospitals. In theUnited States, approximately 5 percent of hospital isolates of S. aureusare methicillin-resistant.

[0020] Thus, there continues to exist a need for new agents useful fortreating bacterial infections, particularly those caused byantibiotic-resistant bacteria, and for methods of identifying such newagents. Such methods ideally would identify agents that are unrelated toexisting antimicrobials and that target different aspects ofstaphylococcal invasion of and replication in the host, compared toexisting antimicrobials.

SUMMARY OF THE INVENTION

[0021] The present invention relates generally to the identification ofgenes responsible for the virulence of Staphylococcus bacteria, therebyallowing the identification of new anti-bacterial agents that targetthese virulence genes and their products and the provision of novel S.aureus mutants useful in vaccines.

[0022] According to one aspect of the present invention, methods areprovided for identifying anti-bacterial agents that target the functionof staphylococcal virulence genes or gene products. Such methods includeassaying potential agents for the ability to interfere with expressionof virulence gene products represented by the DNA sequences set forth inany one of SEQ ID NOS: 1 through 94, or assaying potential agents forthe ability to interfere with the function of a bacterial proteinencoded in whole or in part by a DNA sequence set forth in any one ofSEQ ID NOS: 1 through 94 or the complementary strand thereof, followedby identifying agents that are positive in such assays.

[0023] The use of a number of different assays is contemplated accordingto this aspect of the invention. When the function of the virulence geneproduct is known or predicted by sequence similarity to a known geneproduct, potential inhibitors can be screened in enzymatic or othertypes of assays keyed to the function of the gene product. When thevirulence gene product is known or predicted by sequence similarity to aknown gene product to interact with another protein or nucleic acid,inhibitors of this interaction can be screened directly in bindingassays or using the two-hybrid assay. Other assays may be used when aligand for the virulence gene product is not known, including two-hybridscreening assays that identify gene products that interact with targetprotein, assays that identify ligands of target protein throughmeasuring of direct binding of test ligand to target protein, and assaysthat identify ligands of target proteins through affinityultrafiltration with ion spray mass spectroscopy/HPLC methods or otherphysical and analytical methods.

[0024] In another aspect of this invention, methods are provided forassaying potential agents for the ability to interfere with expressionof or function of virulence gene products, wherein the virulence genesencoding these products are obtainable by identification throughsignature-tagged mutagenesis as defined herein and exemplified inExample 1.

[0025] According to a further aspect of this invention, novelanti-bacterial agents identified by the methods described herein areprovided, as well as methods for treating a subject suffering frominfection with staphylococci involving administration of such novelanti-bacterial agents. In particular, agents that interfere with theexpression of virulence gene products include anti-sense polynucleotidesthat are complementary to the virulence gene sequences. Agents thatinterfere with the function of virulence gene products include variantsof virulence gene products, ligands of these virulence gene products andvariants thereof, and enzyme inhibitors (where the product is anenzyme).

[0026] Yet a further aspect of this invention provides Staphylococcusaureus organisms containing a functional mutation in a gene representedby any one of SEQ ID NOS: 1 through 94, said functional mutationresulting in a reduction in virulence of the organism. Also contemplatedare vaccine compositions comprising such mutated S. aureus organisms,optionally comprising a suitable adjuvant and a pharmaceuticallyacceptable diluent or carrier.

[0027] Numerous additional aspects and advantages of the invention willbecome apparent to those skilled in the art upon consideration of thefollowing detailed description of the invention which describespresently prepared embodiments thereof.

DETAILED DESCRIPTION OF THE INVENTION

[0028] “Virulence genes,” as used herein, are genes whose function orproducts are required for successful establishment and/or maintenance ofbacterial infection in a host animal. Thus, virulence genes and/or theproteins encoded thereby are involved in pathogenesis in the hostorganism, but may not be necessary for growth in vitro. Sinceantibiotics are typically screened in vitro, identification of these invivo virulence genes provides a means for discovering new antimicrobialswith different targets and mechanisms of action compared to existingantibiotics. There may be 50 to 100 virulence genes in S. aureus [seeGroisman and Ochman, Trends Microbiol. Sci., 2:289-294 (1984)(discussing Salmonella virulence genes); Muhldorfer and Hacker, Microb.Pathogenesis, 16:171-181 (1994) (discussing E. coli virulence genes].

[0029] “Signature-tagged mutagenesis,” as used herein, is a methodgenerally described in International Patent Publication No. WO 96/17951,incorporated herein by reference, and includes, for example, a methodfor identifying S. aureus genes required for virulence in a murine modelof bacteremia. In this method, each insertional mutation carries adifferent DNA signature tag which allows mutants to be differentiatedfrom each other. The tags comprise 40-bp variable central regionsflanked by invariant “arms” of 20-bp which allow the central portions tobe co-amplified by polymerase chain reaction (PCR). Tagged mutantstrains are assembled in microtitre dishes, then combined to form the“inoculum pool” for infection studies. At an appropriate time afterinoculation, bacteria are isolated from the animal and pooled to formthe “recovered pool.” The tags in the recovered pool and the tags in theinoculum pool are separately amplified, labelled, and then used to probefilters arrayed with the different tags representing the mutants in theinoculum. Mutants with attenuated virulence are those with tags thatgive hybridization signals when probed with tags from the inoculum poolbut not when probed with tags from the recovered pool.

[0030] Signature-tagged mutagenesis allows a large number of insertionalmutant strains to be screened simultaneously in a single animal for lossof virulence. Screening thirteen pools of 96 mutant S. aureus strainsresulted in the identification of 50 strains with reduced virulence,many of which were confirmed to be attenuated in virulence by subsequentanalysis of individual mutants. The nucleotide sequences of the regionsflanking the transposon insertion points of these mutants were analyzedby searching DNA and protein sequence databases to identify the genesinactivated by the insertion of the transposon.

[0031] On the basis of these searches many of the virulence genes may begrouped into different classes. The first class encodes proteinsinvolved in cell surface metabolism (e.g., P2C73, P11C29, P13C83, P9B65,P10B89). Both femA and femB, which are involved in the formation of cellwall peptidoglycan pentaglycine cross bridges, were identified asvirulence genes. Mutant P2C73 contains a transposon insertion in apreviously unknown gene that shares significant similarity to femB.Mutant P14C15 contains a transposon insertion in a gene whose product issimilar to aspartate semialdehyde dehydrogenase (Asd) from differentbacteria, with the highest level of similarity to Asd from Bacillussubtilis. Asd is a key enzyme in the biosynthesis of methionine,threonine, isoleucine, lysine and diaminopimelic acid (DAP), which is animportant component of cell wall peptidoglycan.

[0032] The second class encodes enzymes involved in cellularbiosynthetic pathways (e.g., P9B74, P5C4, P9B66, P14C15, P13B26, P7C18,P15C31, P10B18, P6B18, P10B66, P10C34, P12C3). Deduced protein productsof two genes (mutants P7C18 and P13B26) show strong similarity to B.subtilis LysA and ThrB. These enzymes, like Asd, are involved inaspartate metabolism. LysA is diaminopimelate decarboxylase, whichconverts diaminopimelate to lysine, and ThrB phosphorylates homoserinebefore conversion into threonine. Transposon insertions were alsoobtained in genes homologous to Methanococcus jannaschii trpA,Lactococcus lactis trpB and L. lactis trpD. These genes encode enzymesof the tryptophan biosynthetic pathway: the alpha chain of tryptophansynthetase, the beta chain of tryptophan synthetase, and anthranilatephosphoribosyltransferase, respectively. The gene mutated in P15C31 is ahomolog of L. lactis purL encoding phosphoribosylformylglycinamidinedecarboxylase, an enzyme of the purine biosynthetic pathway. MutantP9B66 contains an insertion in a gene whose product is similar topeptide methionine sulphoxide reductases.

[0033] A third class of genes are those encoding components of the TCAcycle (e.g., mutants P4C27, P4C52, P10B2, P10C20, P12C32). Strains P10B2and P12C32 carry mutations in genes for a subunit of the oxoglutaratedehydrogenase complex and aconitase, respectively.

[0034] The fourth class is composed of genes whose products are similarto a oligopeptide transport proteins of the ATP-binding cassette (ABC)transporter superfamily (e.g., mutants P7C26, P10C15, P5C3, P11C66,P5C34). Oligopeptide transport is important for peptide utilization andthe proteolytic system in lactococci. In Group A streptococci, Oppproteins are involved not only in obtaining nutrients but also inadherence, protease production and processing of secreted proteins.

[0035] The fifth class of genes are involved in cellular regulatory andrepair processes (e.g., mutants P4C15, P13B74, P13C72, P10B30, P6C63,P14B25). Mutant P4C15 and P6C63 contain insertions in S. aureusMarR/LuxR-like regulatory proteins. MarR and LuxR belong to a family oftranscription regulators and these MarR/LuxR-like proteins likely have asimilar function in S. aureus. In Streptococcus pneumoniae, Neisseriagonorrhoeae and Escherichia coli this enzyme helps to maintain surfaceadhesins in their functional oxidative state. Mutant P10B30 isassociated with a transposon insertion in a gene with a product similarto the ATP-dependent Clp protease of E. coli. The Clp stress responsesystem for intracellular protein degradation is widely conserved inbacteria and components of the system are important for virulence ofListeria monocytogenes and S. typhimurium. Mutants P13B74 and P13C72have stem-loop termination sequences which possibly function intranscription termination.

[0036] The identification of these genes as virulence genes renders themuseful in methods of identifying new anti-bacterial agents according tothe present invention. Such methods include assaying potential agentsfor the ability to interfere with expression of virulence gene productsrepresented by the DNA sequences set forth in any one of SEQ ID NOS: 1through 94 (i.e., the genes represented by DNA sequences of SEQ ID NOS:1 through 94 encode the virulence gene product, or the DNA sequences ofSEQ ID NOS: 1 through 94 are adjacent to the gene encoding the virulencegene product, or are involved in regulation of expression of thevirulence gene product), or assaying potential agents for the ability tointerfere with the function of a bacterial protein encoded in whole orin part by a DNA sequence set forth in any one of SEQ ID NOS: 1 through94 or the complementary strand thereof, followed by identifying agentsthat are positive in such assays. Polynucleotides and polypeptidesuseful in these assays include not only the genes and encodedpolypeptides as disclosed herein, but also variants thereof that havesubstantially the same activity as the wild-type genes and polypeptides.“Variants,” as used herein, includes polynucleotides or polypeptideswhich contain one or more deletions, insertions or substitutions, aslong as the variant retains substantially the same activity of thewild-type polynucleotide or polypeptide. With regard to polypeptides,deletion variants are contemplated to include fragments lacking portionsof the polypeptide not essential for biological activity, and insertionvariants are contemplated to include fusion polypeptides in which thewild-type polypeptide or fragment thereof have been fused to anotherpolypeptide.

[0037] The virulence genes may be cloned by PCR, using S. aureus genomicDNA as the template. For ease of inserting the gene into expressionvectors, PCR primers are chosen so that the PCR-amplified gene has arestriction enzyme site at the 5′ end preceding the initiation codonATG, and a restriction enzyme site at the 3′ end after the terminationcodon TAG, TGA or TAA. If desirable, the codons in the gene are changed,without changing the amino acids, according to E. coli codon preferencedescribed by Grosjean and Fiers, Gene, 18:199-209 (1982), and Konigsbergand Godson, Proc. Natl. Acad. Sci. (USA), 80:687-691 (1983).Optimization of codon usage may lead to an increase in the expression ofthe gene product when produced in E. coli. If the gene product is to beproduced extracellularly, either in the periplasm of E. coli or otherbacteria, or into the cell culture medium, the gene is cloned withoutits initiation codon and placed into an expression vector behind asignal sequence. For example, cloning and expression of the femA gene isdescribed in Example 3 below.

[0038] To simplify the protein purification process, a purification tagmay be added either at the 5′ or 3′ end of the gene coding sequence.Commonly used purification tags include a stretch of six histidineresidues (U.S. Pat. Nos. 5,284,933 and 5,310,663), astreptavidin-affinity tag described by Schmidt and Skerra, ProteinEngineering, 6:109-122 (1993), a FLAG peptide [Hopp et al.,Biotechnology, 6:1205-1210 (1988)], glutathione S-transferase [Smith andJohnson, Gene, 67:31-40 (1988)], and thioredoxin [LaVallie et al.,Bio/Technology, 11:187-193 (1993)]. To remove these peptide orpolypeptides, a proteolytic cleavage recognition site may be inserted atthe fusion junction. Commonly used proteases are factor Xa, thrombin,and enterokinase.

[0039] Proteins are produced in any number of well-known prokaryotic oreukaryotic expression systems using known promoters, vectors, and hosts.Any suitable host cell may be used for expression of the gene product,such as E. coli, other bacteria, including Bacillus and S. aureus,yeast, including Pichia pastoris and Saccharomyces cerevisiae, insectcells, or mammalian cells, including CHO cells, utilizing suitablevectors known in the art. Proteins may be produced directly or fused toa peptide or polypeptide, and either intracellularly or extracellularlyby secretion into the periplasmic space of a bacterial cell or into thecell culture medium. Secretion of a protein requires a signal peptide(also known as pre-sequence); a number of signal sequences fromprokaryotes and eukaryotes are known to function for the secretion ofrecombinant proteins. During the protein secretion process, the signalpeptide is removed by signal peptidase to yield the mature protein.

[0040] The virulence gene products produced by the methods describedabove are used in high throughput assays to screen for inhibitoryagents. The sources for potential agents to be screened are chemicalcompound libraries, fermentation media of Streptomycetes, other bacteriaand fungi, and cell extracts of plants and other vegetations. Forproteins with known enzymatic activity, assays are established based onthe activity, and a large number of potential agents are screened forability to inhibit the activity. For proteins that interact with anotherprotein or nucleic acid, binding assays are established to measure suchinteraction directly, and the potential agents are screened for abilityto inhibit the binding interaction.

[0041] Alternatively, such binding interactions are evaluated indirectlyusing the yeast two-hybrid system described in Fields and Song, Nature,340:245-246 (1989), and Fields and Stemglanz, Trends in Genetics,10:286-292 (1994), both of which are incorporated herein by reference.The two-hybrid system is a genetic assay for detecting interactionsbetween two proteins or polypeptides. It can be used to identifyproteins that bind to a known protein of interest, or to delineatedomains or residues critical for an interaction. Variations on thismethodology have been developed to clone genes that encode DNA-bindingproteins, to identify peptides that bind to a protein, and to screen fordrugs. The two-hybrid system exploits the ability of a pair ofinteracting proteins to bring a transcription activation domain intoclose proximity with a DNA-binding domain that binds to an upstreamactivation sequence (UAS) of a reporter gene, and is generally performedin yeast. The assay requires the construction of two hybrid genesencoding (1) a DNA-binding domain that is fused to a protein X, and (2)an activation domain fused to a protein Y. The DNA-binding domaintargets the first hybrid protein to the UAS of the reporter gene;however, because most proteins lack an activation domain, thisDNA-binding hybrid protein does not activate transcription of thereporter gene. The second hybrid protein, which contains the activationdomain, cannot by itself activate expression of the reporter genebecause it does not bind the UAS. However, when both hybrid proteins arepresent, the noncovalent interaction of protein X and protein Y tethersthe activation domain to the UAS, activating transcription of thereporter gene. When the virulence gene product (protein X, for example)is already known to interact with another protein or nucleic acid(protein Y, for example), this assay can be used to detect agents thatinterfere with the interaction of X and Y. Expression of the reportergene is monitored as different test agents are added to the system; thepresence of an inhibitory agent results in lack of a reporter signal.

[0042] When the function of the virulence gene product is unknown and noligands are known to bind the gene product, the yeast two-hybrid assaycan also be used to identify proteins that bind to the gene product. Inan assay to identify proteins that bind to protein X (the targetprotein), a large number of hybrid genes each containing a differentprotein Y are produced and screened in the assay. Typically, Y isencoded by a pool of plasmids in which total cDNA or genomic DNA isligated to the activation domain. This system is applicable to a widevariety of proteins, and it is not even necessary to know the identityor function of protein Y. The system is highly sensitive and can detectinteractions not revealed by other methods; even transient interactionsmay trigger transcription to produce a stable mRNA that can berepeatedly translated to yield the reporter protein.

[0043] Other assays may be used to search for agents that bind to thetarget protein. One such screening method to identify direct binding oftest ligands to a target protein is described in U.S. Pat. No.5,585,277, incorporated herein by reference. This method relies on theprinciple that proteins generally exist as a mixture of folded andunfolded states, and continually alternate between the two states. Whena test ligand binds to the folded form of a target protein (i.e., whenthe test ligand is a ligand of the target protein), the target proteinmolecule bound by the ligand remains in its folded state. Thus, thefolded target protein is present to a greater extent in the presence ofa test ligand which binds the target protein, than in the absence of aligand. Binding of the ligand to the target protein can be determined byany method which distinguishes between the folded and unfolded states ofthe target protein. The function of the target protein need not be knownin order for this assay to be performed. Virtually any agent can beassessed by this method as a test ligand, including, but not limited to,metals, polypeptides, proteins, lipids, polysaccharides, polynucleotidesand small organic molecules. For example, use of femA in this method ofscreening for potential ligands is described in Example 3 below.

[0044] Another method for identifying ligands for a target protein isdescribed in Wieboldt et al., Anal. Chem., 69:1683-1691 (1997),incorporated herein by reference. This technique screens combinatoriallibraries of 20-30 agents at a time in solution phase for binding to thetarget protein. Agents that bind to the target protein are separatedfrom other library components by centrifugal ultrafiltration. Thespecifically selected molecules that are retained on the filter aresubsequently liberated from the target protein and analyzed by HPLC andpneumatically assisted electrospray (ion spray) ionization massspectroscopy. This procedure selects library components with thegreatest affinity for the target protein, and is particularly useful forsmall molecule libraries.

[0045] The inhibitors/binders identified by the initial screens areevaluated for their effect on virulence in in vivo mouse models of S.aureus infections. Models of bacteremia, endocarditis, septic arthritis,soft tissue abscess or pneumonia may be utilized. Inhibitors/bindersthat interfere with bacterial virulence are capable of preventing theestablishment of an infection or reversing the outcome of an infectiononce it is established.

[0046] The identification of S. aureus virulence genes also provides formicroorganisms exhibiting reduced virulence, which are useful invaccines. Such microorganisms include the S. aureus mutants generated inExample 1 below and other S. aureus mutants containing at least onefunctional mutation in a gene represented by any one of SEQ ID NOS: 1through 94. The reduced virulence of these organisms and theirimmunogenicity may be confirmed by administration to a subject. While itis possible for an avirulent microorganism of the invention to beadministered alone, one or more of such mutant microorganisms arepreferably administered in a vaccine composition containing suitableadjuvant(s) and pharmaceutically acceptable diluent(s) or carrier(s).The carrier(s) must be “acceptable” in the sense of being compatiblewith the avirulent microorganism of the invention and not deleterious tothe subject to be immunized. Typically, the carriers will be water orsaline which will be sterile and pyrogen free. The subject to beimmunized is a subject needing protection from a disease caused by avirulent form of S. aureus.

[0047] Any adjuvant known in the art may be used in the vaccinecomposition, including oil-based adjuvants such as Freund's CompleteAdjuvant and Freund's Incomplete Adjuvant, mycolate-based adjuvants(e.g., trehalose dimycolate), bacterial lipopolysaccharide (LPS),peptidoglycans (i.e., mureins, mucopeptides, or glycoproteins such asN-Opaca, muramyl dipeptide [MDP], or MDP analogs), proteoglycans (e.g.,extracted from Klebsiella pneumoniae), streptococcal preparations (e.g.,OK432), Biostim™ (e.g., 01K2), the “Iscoms” of EP 109 942, EP 180 564and EP 231 039, aluminum hydroxide, saponin, DEAE-dextran, neutral oils(such as miglyol), vegetable oils (such as arachis oil), liposomes,Pluronic® polyols or the Ribi adjuvant system (see, for example GB-A-2189 141). Recently, an alternative adjuvant consisting of extracts ofAmycolata, a bacterial genus in the order Actinomycetales, has beendescribed in U.S. Pat. No. 4,877,612. Additionally, proprietary adjuvantmixtures are commercially available. The adjuvant used will depend, inpart, on the recipient organism. The amount of adjuvant to administerwill depend on the type and size of animal. Optimal dosages may bereadily determined by routine methods.

[0048] The vaccine compositions optionally may include pharmaceuticallyacceptable (i.e., sterile and non-toxic) liquid, semisolid, or soliddiluents that serve as pharmaceutical vehicles, excipients, or media.Any diluent known in the art may be used. Exemplary diluents include,but are not limited to, polyoxyethylene sorbitan monolaurate, magnesiumstearate, methyl- and propylhydroxybenzoate, talc, alginates, starches,lactose, sucrose, dextrose, sorbitol, mannitol, gum acacia, calciumphosphate, mineral oil, cocoa butter, and oil of theobroma.

[0049] The vaccine compositions can be packaged in forms convenient fordelivery. The compositions can be enclosed within a capsule, sachet,cachet, gelatin, paper or other container. These delivery forms arepreferred when compatible with entry of the immunogenic composition intothe recipient organism and, particularly, when the immunogeniccomposition is being delivered in unit dose form. The dosage units canbe packaged, e.g., in tablets, capsules, suppositories or cachets.

[0050] The vaccine compositions may be introduced into the subject to beimmunized by any conventional method including, e.g., by intravenous,intradermal, intramuscular, intramammary, intraperitoneal, orsubcutaneous injection; by oral, sublingual, nasal, anal, vaginal, ortransdermal delivery; or by surgical implantation, e.g., embedded underthe splenic capsule or in the cornea. The treatment may consist of asingle dose or a plurality of doses over a period of time.

[0051] It will be appreciated that the vaccine of the invention may beuseful in the fields of human medicine and veterinary medicine. Thus,the subject to be immunized may be a human or an animal, for example,cows, sheep, pigs, horses, dogs and cats, and poultry such as chickens,turkeys, ducks and geese.

[0052] Other aspects and advantages of the present invention will beunderstood upon consideration of the following illustrative examples.Example 1 addresses identification of S. aureus genes associated withvirulence by generating S. aureus mutants that contain a chromosomalinsertion of a signature-tagged transposon and identifying the mutantswith reduced virulence. Example 2 addresses confirmation of theattenuated virulence of individual mutants. Example 3 addresses use ofthe virulence genes and gene products in assays for screening potentialagents for anti-bacterial activity.

EXAMPLE 1 Identification of S. aureus Genes Associated with Virulence

[0053]S. aureus genes associated with virulence were identified bysignature-tagged mutagenesis as follows, generally according toInternational Patent Publication No. WO 96/17951 and Hensel et al.,Science, 269:400-403 (1995).

[0054] A. Construction of Plasmid pID408

[0055] A temperature-sensitive shuttle plasmid pID408 was constructedfor use in transferring the signature-tagged transposons into S. aureus.DNA restriction digestions and ligations were performed as described bySambrook et al., Molecular cloning: a laboratory manual, 2nd ed., ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989). A 6.5kb BamHI fragment of pTV32ts [described in Youngman, in Plasmids: apractical approach, K. Hard, ed., Oxford: IRL Press, pages 79-103(1985); obtained from J. Iandolo, Department of Diagnostic Medicine,College of Veterinary Medicine, Manhattan, Kans.] carrying thetemperature-sensitive replicon pE194ts and a chloramphenicol (cm)resistance gene (cm^(R)) was inserted into the BamHI site of plasmidpSP72 (Promega), forming pID402. Plasmid pID402 was digested with SmaIand self-ligated to remove a small fragment containing a BamHI sitebetween the SmaI sites, resulting in pID405. A DNA fragment of pBR322(New England BioLabs) containing the ampicillin resistance gene(amp^(R))/ori/rop region (corresponding to bases 1904-4359 of pBR322)was prepared by polymerase chain reaction (PCR) amplification usingprimers pBR1 (5′-GGAGCTCACTAGTCGGAGGCATCAGTGACC-3′, SEQ ID NO: 95) andpBR2 (5′-GGGATCCCATGAGAATTCTTGAAGACG-3′, SEQ ID NO: 96). Primer pBR1contains sites for SacI and SpeI, and pBR2 contains sites for BamHI andEcoRI. The PCR product was digested with BamRI and SacI and ligated tothe BamHI/SacI-digested fragment of pID405 (carrying replicon pE194tsand cmR) to create pID406, in which the amp^(R)/ori region of pSP72 isreplaced with the replicon of pBR322. Finally, a 6.5 kb BamHI-EcoRIfragment from pTV9 [described in Youngman, in Plasmids: a practicalapproach, K. Hard, ed., Oxford: IRL Press, pages 79-103 (1985)],containing transposon Tn917 and its erythromycin (erm) resistance gene(erm^(R)), was ligated with BamHI and EcoRI digested pID406, resultingin plasmid pID408. The origin of replication from pBR322 allows pID408to replicate in E. coli, and the temperature-sensitive replicon pE194ts(from pTV32ts) allows replication in S. aureus at 32° C.

[0056] B. Cloning and Selection of Signature Tags

[0057] DNA signature tags were prepared as described by Hensel et al.,Science, 269:400-403 (1995) and inserted into the transposon Tn917carried by pID408 as follows. Oligonucleotides tags in pool RT1 (5′-C TA G G T A C C T A C A A C C T C A A G C T T-[NK]₂₀-AAGCTTGGTTAGAATGGGTACCATG-3′, SEQ ID NO: 97, where N is A, C, G orT, and K is G or T were synthesized on an oligonucleotide synthesizer(Applied Biosystems). The 80 bp tags from oligonucleotide pool RT1 werePCR-amplified from the using primers P10(5′-CTAGAATTCTACAACCTCAAGCTT-3′, SEQ ID NO: 98) and P11(5′-AAGCTTGGTTAGAATGGAATTCATG-3′, SEQ ID NO: 99). The PCR-amplified tagswere digested with EcoRI, gel-purified, and ligated with EcoRI digested,dephosphorylated pID408, to form plasmids containing uniquelysignature-tagged Tn917 transposons. The ligated DNA was transformed intoS. aureus strain RN4220 (a restriction defective strain derived fromNCTC 8325-4 and described in Youngman, in Plasmids: a practicalapproach, K. Hard, ed., Oxford: IRL Press, pages 79-103 (1985) byelectroporation. Electroporation was performed by the method of Schenkand Laddaga, FEMS Microbiol. Lett., 94:133-138 (1992), with thefollowing modifications: electrotransformed bacteria were plated onnon-selective brain-heart infusion (BH, Difco) agar plates (1.5% agar),grown at 32° C. for 8 to 12 hours and then replicated using an AccutranReplica Plater (Schleicher and Schuell) onto BHI agar plates containing20 μg/ml erm and 20 μg/ml cm, and grown at 32° C. overnight.

[0058] A total of 400 erm^(R), cm^(R) transformants were then screenedto identify 150 tags that amplified and labelled efficiently. The 150transformants were each cultured in 10 ml BHI broth containing 20 μg/mlerm and 20 μg/ml cm at 32° C. overnight. Plasmid DNA was extracted fromeach culture using a Qiagen Plasmid Miniprep Kit according to themanufacturer's protocol except that the bacterial cells were lysed bylysostaphin (Sigma; 200 mg/ml) at 37° C. for 30 min. in the P1 solutionof the Qiagen miniprep kit. An aliquot of each plasmid DNA preparation(1 μg) was transferred onto a Hybond N⁺ membrane (Amersham) by using aBio-Dot Microfiltration Apparatus according to the manufacturer'sprotocol (Bio-Rad) to give 75 different plasmids per membrane. Themembranes were then transferred to Whatman 3MM paper saturated withdenaturing solution (0.5 N NaOH, 1.5 M NaCl), incubated for 5 min. thentransferred to another piece of Whatman 3MM paper saturated withneutralizing solution (1.5 M NaOH, 0.5 M Tris-Cl pH 7.4) for 5 min.After neutralization, the membranes were dried at 80° C. for 10 min andthe DNA cross-linked by UV light using a Stratalinker (Stratagene). Thesignature tags from the 150 transformants were also labelled for use asprobes. Aliquots of the plasmid preparations were combined to form fourpools of 37, 37, 38 and 38 plasmids. The DNA signature tags from thesefour pools were PCR-amplified with primers P12 (5′-GAATTCCATTCTAAC-3′,SEQ ID NO: 100) and P13 (5′-ATTCCATTCTAACCAAGC-3′, SEQ ID NO: 101)according to Hensel et al., Science, 269:400-403 (1995). These PCRproducts were gel-purified, subjected to a digoxygenin (DIG)-labellingPCR using a PCR DIG Probe Synthesis Kit as described by the manufacturer(Boehringer Mannheim). The DIG-labelled PCR products were hybridizedseparately with the membranes according to the method described in theDIG Probe Synthesis Kit, to identify tags that gave strong hybridizationsignals, but which did not cross-hybridize with tags in the other pools.From these experiments, a total of 96 plasmids were chosen on the basisof labelling efficiency of tags and lack of cross-hybridization.

[0059] C. Generation of the S. aureus Mutant Bank

[0060] The 96 plasmids containing uniquely signature tagged-transposonswere used to generate S. aureus mutants with a chromosomal transposoninsertion. A series of identical membranes for dot-blot hybridizationswere prepared by transferring 1 μg of each of the 96 plasmids ontoHybond N⁺ membranes using the Bio-Dot Microfiltration Apparatus. The 96plasmids were separately transformed into S. aureus strain RN6390 byelectroporation [Schenk and Laddaga, FEMS Microbiol. Lett., 94:133-138(1992)]. Electrotransformed bacteria were plated directly on BHI agarplates containing 20 μg/ml cm and 20 μg/ml erm at 32° C. overnight. Asingle colony from each transformation was transferred into each well ofa microtitre dish containing 200 μl BHI broth, 20 μg/ml erm and 20 μg/mlcm. The microtitre dish was incubated at 32° C. overnight. Glycerol wasadded to each well to give a final concentration of 50% and the plateswere stored at −80° C. This collection of 96 transformants wasdesignated the master pool and was used for all subsequent mutagenesis.

[0061] To generate 96 different Tn917 mutants, bacteria from the masterpool were replicated using a microtitre dish replicator (Sigma) into thewells of a new microtitre dish containing 200 μl BHI broth and 20 μg/mlerm. This dish was incubated at 43° C. overnight and then bacteria fromeach well were streaked on BHI agar containing 20 μg/ml erm andincubated at 43° C. overnight to obtain single colonies. Approximatelyfive different erm^(R) colonies obtained from each well were transferredonto BHI agar containing 20 μg/ml cm and incubated at 32° C., and ontoBHI agar containing 20 μg/ml erm and incubated at 43° C., to screen forcm^(S) colonies. Erm^(R), cm^(S) colonies should lack the plasmid andcarry a chromosomal insertion of Tn917. Individual erm^(R), cm^(S)colonies from each of the 96 wells were transferred into a new 96 wellmicrotitre dish with BHI broth containing 20 μg/ml erm and grownovernight at 43° C. for use as inoculum. These 96 mutants, takentogether, form one “inoculum pool.” Mutant pools were also stored at−80° C. in 50% glycerol.

[0062] To verify that erm^(R), cm^(S) colonies lack the plasmid andcarry a single chromosomal insertion of Tn917, chromosomal DNA samplesfrom individual mutant strains and from a pool of 46 colonies that hadbeen cultured separately were digested with EcoRI and subjected toSouthern analysis using a fragment of the β-lactamase gene as a probe.For each of 12 separately analyzed mutants, a single hybridizingfragment of different size was observed in each lane. A large number ofhybridizing fragments were observed in the lane containing DNA from 46mutants. These results indicate that the majority of Tn917 insertionsoccur singly and at different locations in the S. aureus chromosome.

[0063] D. Infection Studies to Identify Mutants with Reduced Virulence

[0064] The “inoculum pool” of 96 S. aureus mutants containingchromosomal transposon insertions was evaluated for attenuated virulencein a mouse model of bacteremia. After overnight incubation at 43° C.,the 96 different mutants from individual wells of the microtitre dishwere pooled together and washed twice with BHI broth by centrifugationat 4000×g for 10 min and resuspended in BHI broth. The OD₆₂₀ wasdetermined using a spectrophotometer (OD₆₂₀ of 1.6×10⁹ colony formingunits (CFU)/ml) [Cheung et al., J. Bacteriol., 177:3220-3226 (1995).].The bacterial suspension was diluted to approximately 5×10⁶ CFU/ml andthen mixed with an equal volume of pre-autoclaved 4% (w/v) Brewer'syeast (Sigma) in BHI broth. A total of 0.2 ml of this mixture,containing approximately 5×10⁵ CFU bacteria, was injectedintraperitoneally into a CD-1 mouse (approximately 25 g in weight). Thenumber of CFU in the inoculum was verified by viable counts afterplating a diluted aliquot of the inoculum to BHI agar. Two to four micewere inoculated with each pool. 48 hours after inoculation, bacteriawere recovered from the spleens of the animals as described by Hensel etal., Science, 269:400-403 (1995) and pooled to form the “recoveredpool.” Each recovered pool was made from at least 10,000 bacterialcolonies. The signature tags present in the recovered pools werecompared with the signature tags present in the inoculum pools by PCRamplifying the tags using primers P12 and P13 (described in section Babove), DIG labelling the tags, and hybridizing the labelled tags to the96 plasmids which had been transferred to Hybond N⁺ membranes using theBio-Dot Microfiltration Apparatus as described above in section C.

[0065] E. Virulence Gene Identification and DNA Sequencing

[0066] A total of 13 pools, each comprising 96 S. aureus Tn917 mutants,were screened as described above for loss of virulence in mice. Fromthese, 50 mutants were identified whose tags hybridized strongly toprobes from the inoculum pools but weakly to probes from thecorresponding recovery pools.

[0067] To clone the chromosomal DNA flanking the transposon insertionpoints of these 50 mutants, 3-5 μg of S. aureus chromosomal DNA fromeach mutant was isolated as described by Pospiech and Neumann, TrendsGenetc., 11:217-218 (1995) and completely digested with HindIII. Half ofthe digested DNA was then subjected to Southern hybridization analysisusing the pBR322 fragment of pID408 as a probe to determine the size ofthe DNA fragment carrying this part of the transposon. The rest of theDNA was resuspended in 200 μl of ligation buffer (Gibco-BRL) andself-ligated overnight at 16° C. The ligated products were transformedinto E. coli DH5a (Gibco) and plated onto Luria Bertani (LB) agarcontaining 50 μg/ml ampicillin, and incubated at 37° C. overnight. Asingle amp^(R) colony from each transformation was grown up in LB brothcontaining 100 μg/ml amp. Plasmid DNA was extracted using a Qiagenplasmid miniprep kit. Chromosomal DNA flanking the transposon wasobtained using primer pseq-1 (5′-TGAACTGCCACTGTAGAGAGA-3′, SEQ ID NO:102) based on the erm-proximal end of Tn917, and sequenced (for astretch of several hundred nucleotides) using a Model 373A SequencingSystem (Applied Biosystems). The sequence obtained is indicated in Table1 below with reference to the corresponding nucleotides of the SEQ IDNO:______.

[0068] These DNA sequences were analyzed by searching the S. aureusdatabase from Human Genome Sciences and the European Molecular BiologyLaboratory and Genbank DNA and Protein Databases using the BLAST andFASTA network service at the Human Genome Mapping Project RecourseCentre, Hinxton, UK. Results of the searches done to date in identifyingthe virulence genes and their possible function are shown in Table 1.Table 1 below displays the signature tag identification number, the SEQID NO: corresponding to each virulence gene (and the nucleotidepositions within the SEQ ID NO: that correspond to the actual stretch ofDNA that was sequenced), the possible function of the gene, and the LD₅₀of the knockout mutant as determined in Example 2 below.

EXAMPLE 2 Assessment of Virulence of S. aureus Knockout Mutants

[0069] The virulence of individual knockout mutants, identified inExample 1 as having reduced virulence when tested in pools of 96mutants, was assessed by carrying out an LD₅₀ determination for eachmutant. Confirmation that a given mutation is in a virulence gene may beobtained by comparing the parent and mutated bacterial strains in termsof whether both strains are equally effective in establishing aninfection with the same consequences for the host animal. In practice,this may be done by comparing LD₅₀ values (the number of bacteriarequired to produce a 50% mortality rate in the animals understandardized conditions) between the wild-type strain and the mutantderivative strain. If the LD₅₀ values are within standard error of eachother then the mutation is not in or does not affect a virulence gene.If significant differences occur between the wild-type and the mutantstrain, where the mutant strain is significantly less able to causelethal infection, then the mutation is in a virulence gene ordramatically affects a virulence gene.

[0070] Briefly, LD₅₀ determinations were conducted as follows. CF1female mice weighing between 19 and 22 grams were injectedintraperitoneally with a set concentration of either wild-type or mutantS. aureus in 0.2 ml of BHI broth containing 4% (w/v) dried brewer'syeast as an adjuvant for establishment of infection. The amount ofbrewer's yeast may vary from 0-8% dependent upon the bacteria beingutilized in the infection. A single LD₅₀ determination utilizes fivelog-dilutions of bacteria (10³ to 10⁷), with each log-dilution beingtested in 10 mice. The actual number of bacteria employed was estimatedin each LD₅₀ determination by a plate count conducted on the bacterialstock to determine the number of CFU of bacteria. Following infection,the mice were monitored daily for mortality for a period of at least oneweek. At the end of the observation period the LD₅₀ was determined usingprobit analysis on the mortality data. The LD₅₀ value of the wild-typeS. aureus strain RN6390 ranges from 1.4×10⁴ to 1.4×10⁵. The LD₅₀ valuesfor the virulence gene mutants are shown in Table 1 below. TABLE 1VIRULENCE GENE SEQUENCES AND POSSIBLE FUNCTION Position SEQ ID of DNANO: sequence LD₅₀ of SIGNA- (DNA; obtained IDENTIFICATION knockout TUREamino in OF POSSIBLE mutant TAG ID acid) Example1 FUNCTION OF GENE(#CFU)* p2c73 SEQ ID 517-756 FemA/B like with ˜40% >6.8 × NO: 1;identity 10⁸ 2 p2c90 SEQ ID 281-431 Unknown; near Opp operon NO: 3 p9b74SEQ ID 593-711 Tryptophan synthase alpha NO: 4; chain TrpA 5 p11c29 SEQID 1107-1260 FemB NO: 6; 7 p13c83 SEQ ID 1107-1260 FemB  3.9 × NO: 6;10⁵ 7 p4c15 SEQ ID 360-654 MarR/LuxR-like regulatory NO: 8; protein 9 &10 p6c63 SEQ ID 388-659 MaxR/LuxR-like regulatory >5.4 × NO: 8; protein10⁸ 9 & 10 p5c4 SEQ ID 192-481 41% identity to nitrate NO: 11; reductaseNirQ 12 p9b66 SEQ ID 221-474 Peptide methionine  4.0 × NO: 13; sulfoxidereductase 10⁴ 14 p7c26 SEQ ID  44-410 Oligopeptide transporter NO: 15;OppF 16 p10c15 SEQ ID 562-825 Oligopeptide transporter >2.6 × NO: 17OppD 10⁸ & 19; 18 & 20 p13b74 SEQ ID all Possible stem-loop termina- 8.8 × NO: 21 tion sequence; no obvious 10⁴ ORF p13c72 SEQ ID allPossible stem-loop termina- NO: 21 tion sequence; no obvious ORF p14c15SEQ ID  669-1009 C-term of aspartokinase 2 NO: 22; alpha subunit andN-term of 23 & 24 aspartate semialdeyde de- hydrogenase Asd; bothinvolved in homoserine synthesis p13b26 SEQ ID 442-819 homoserine kinase 7.7 × NO: 25; 10⁴ 26 p7c18 SEQ ID 667-847 diaminopimelate de- NO: 27;carboxylase LysA; lysine 28 synthesis p15c31 SEQ ID 1594-2018phosphoribosylformyl- NO: 29; glyinamide decarboxylase 30 PurL; purinesynthesis p10b18 SEQ ID  3-404 tryptophan synthase alpha >5.2 × NO: 31;chain trpA 10⁸ 32 p6b18 SEQ ID  3-404 tryptophan synthase alpha NO: 31;chain trpA 32 p10b66 SEQ ID  30-282 tryptophan synthase beta >4.2 × NO:33; chain trpB 10⁶ 34 p10c34 SEQ ID 609-817 Anthranilate   >4 × NO: 35;phosphoribosyl transferase 10⁶ 36 TrpD; tryptophan synthesis p4c27 SEQID 1130-1254 dihydrolipoamide succinyl NO: 37; transferase component(e2)38 of 2-oxoglutarate dehydro- genase complex in TCA cycle p4c52 SEQ ID498-738 dihydrolipoamide succinyl NO: 39; transferase component(e2) 40of 2-oxoglutarate dehydro- genase complex in TCA cycle p10b2 SEQ ID 880-1159 dihydrolipoamide succinyl >2.6 × NO: 41; transferasecomponent(e2) 10⁸ 42 of 2-oxoglutarate dehydro- genase complex in TCAcycle p10c20 SEQ ID all Sequence 3′ to the  3.7 × NO: 43dihydrolipoamide succinyl 10⁵ transferase component(e2) of2-oxoglutarate dehydro- genase complex in TCA cycle; no obvious ORFp12c32 SEQ ID 188-438 Alpha-ketoglutarate >1.0 × NO: 44; dehydrogenase(e1) of the 10⁶ 45 2-oxoglutarate dehydro- genase complex in TCA cycle;acetyl CoA synthase p10b30 SEQ ID 2419-2574 Heat shock protein ClpB  1.4× NO: 46; 10⁴ 47 p13c3 SEQ ID 454-765 35% identity to hypo-  1.8 × NO:48; thetical 45.9 kDa protein 10⁴ 49 from B. subtilis ImpB/ MucB/SamBfamily p4b3 SEQ ID  790-1203 Unknown; homology to NO: 50; hypotheticalproteins from 51 B. subtilis YAAD and YAAE p4c63 SEQ ID 576-806 Unknown;metallopeptidase NO: 52; motif 53 p5c3 SEQ ID  1-450 Unknown; membranetrans-  7.1 × NO: 54; porter motif 10⁴ 55 p8d26 SEQ ID  32-262 Unknown;38% identity to NO: 56 C. elegans ORF & 58; 57 & 59 p9b65 SEQ ID  1-361sodium/proton dependent >9.2 × NO: 60; alanine carrier protein 10⁸ 61p10b32 SEQ ID 205-296 Unknown NO: 62; 63 p10b85 SEQ ID  38-212 UnknownNO: 64 p10b89 SEQ ID  1-205 Unknown; prokaryotic >2.2 × NO: 65; membranelipoprotein lipid 10⁸ 66 attachment motif p10c30 SEQ ID  730-1041Unknown; sensor-type  8.8 × NO: 67; protein motif 10⁵ 68 p10c52 SEQ ID 1-262 Unknown NO: 69; 70 p10d9 SEQ ID 422-639 Unknown; enterotoxin-likeNO: 71; motif 72 p11c12 SEQ ID  1-150 Unknown; 40% identity to >4.2 ×NO: 73; Mycoplasma mycoides 10⁶ 74 hypothetical protein in ffh 5′ regionq01444 p11c66 SEQ ID 292-529 Unknown; near Opp operon NO: 75 & 77; 76 &78 p5c34 SEQ ID 1180-1236 glycine betaine transporter; NO: 79; region55% identity to B. 80 subtilis p54417 p10c18 SEQ ID  1-477Unknown-potential ORF in NO: 81 120 bp p12c3 SEQ ID  1-318 acetyl-CoAsynthetase; NO: 82; region 59% identity to B. 83 subtilis p39062 p14b25SEQ ID  1-148 Unknown; exonuclease- NO: 84; like; 25% identity to E.coli 85 p13458; contains ATP binding motif p14b74 SEQ ID 243-624Unknown; 51% identity to NO: 86; hypothetical 45.9 kd 87 protein(YQJW)from B. subtilis p54560 p14c13 SEQ ID  1-441 Unknown; near Opp operonNO: 88 p15b9 SEQ ID 654-867 Unknown; 41% identity to NO: 89;hypothetical 33.7 kd protein 90 (YHCT) from B. subtilis p54604 p15b32SEQ ID 286-370 Unknown; similar to orf1 NO: 91; 5′ of acvB of Agro. 92tumefaciens a36922 p15c4 SEQ ID  25-192 4-oxalocrotonate NO: 93;tautomerase; 42% identity 94 to Pseudo. putida a43397

EXAMPLE 3 Use of Virulence Gene Products in Screen for Anti-BacterialAgents

[0071] The virulence genes and their gene products are utilized inassays for identifying new anti-bacterial agents against S. aureus. Thegenes are cloned, the proteins encoded by the genes are produced andpurified, high throughput assays are established to screen forinhibitors, and the inhibitors identified in the primary screen areevaluated in secondary assays. The cloning and expression of FemA, andits use in screening potential inhibitory agents, is described below.

[0072] A. Cloning of FemA Gene

[0073] DNA restriction digestions and ligations are performed asdescribed by Sambrook et al., Molecular cloning: a laboratory manual,2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(1989). Cloning of the S. aureus femA gene [Berger-Bachi et al., Mol.Gen. Genet., 219:263-269 (1989)] for intracellular expression in E. coliis carried out as follows. To clone the gene with six histidine residuesat the 3′-terminus, the following two oligonucleotides primers,5′-GGCCATCGATAATGAAATTAATTAACGAGAGACAAATAGG-3′, SEQ ID NO: 103, and5′-GGCCGGATCCCTAGTGATGGTGATGGTGATGAAAAATTCTGTCTTTAACT TTTTT-3′, SEQ IDNO: 104, are used to PCR amplify the gene from S. aureus genomic DNA.The resulting femA gene has a ClaI site at 5′-end and a BamHI site at3′-end for cloning into E. coli expression vectors. For secretion of thefemA gene with six histidine residues at the 3′-terminus into theperiplasm of E. coli, PCR amplification using the following twooligonucleotide primers, 5′-GGCCGGTACCAAATTAATTAACGAGAGACAAATAGG-3′, andSEQ ID NO: 1055′GGCCGGATCCCTAGTGATGGTGATGGTGATGAAAAATTCTGTCTTTAACTTTTTT-3′, SEQ ID NO:106

[0074] results in a fragment with a KpnI site at the 5′-end and a BamHIsite at the 3′-end. The approximately 1.3 kb PCR fragment may besequenced directly, or after cloning into an expression vector, toconfirm the sequence of the femA gene and its flanking regions.

[0075] B. Recombinant FemA Protein Production and Purification

[0076] Expression of femA in E. coli is carried out as follows. The E.coli expression vector pSRtac-pelB contains the synthetic tac promoterand the signal sequence of PelB [Lei et al., J. Bact., 169:4379-4383(1987)], with transcription terminators upstream and downstream tominimize transcription read through. For intracellular expression, thefemA gene generated by PCR as described above in section A with ClaI andBamHI sites at the termini is cloned into pSRtac-pelB digested with ClaIand BamHI (replacing the PelB signal sequence). For secretion intoperiplasm, the femA gene generated by PCR as described above in sectionA with KpnI and BamHI sites at the termini is cloned into pSRtac-pelBdigested with KpnI and BamHI, directly downstream from the pelB signalsequence. E. coli are transformed with the expression vector containingthe femA gene. To induce the tac promoter and optimize expression level,the transformed bacteria are incubated with IPTG(isopropylthio-β-galactoside) at concentrations ranging from 2×10⁻³ M to1×10⁻⁵ M, at cell growth temperatures between 27° C. to 42° C. in richmedium or minimal medium.

[0077] The recombinant FemA thus produced contains six histidineresidues at the C-terminus and can be purified via the affinity ofhistidine to nickel. Chromatography may be carried out with the metalchelating resin Ni-nitrilotriacetic acid (Ni-NTA) (available fromQiagen, Chatsworth, Calif.) under native or denaturing conditions.

[0078] C. High Throughput Assays Using FemA for Screening PotentialInhibitors

[0079] The screening method described in U.S. Pat. No. 5,585,277 is usedas follows to establish a high throughput assay to identify ligands thatbind to FemA. The test ligand and femA are combined under conditions(e.g., temperature, pH, salt concentration, time) appropriate fordetecting binding of femA to a ligand. If too little target protein isunfolded, the observed signal will occur at too low a level or rate tobe conveniently measured. The conditions are optimized using knownmethods. Binding of a test ligand to femA is assessed in one of severalways: by determining the extent to which folded femA is present in thetest ligand-femA combination, by determining the extent to whichunfolded femA is present in the test ligand-femA combination, or bydetermining the ratio of folded femA to unfolded femA in thecombination. There are numerous methods to carry out thesedeterminations. For example, proteolysis may be used. A protease whichacts preferentially on unfolded femA is combined with the testligand-femA combination, and after an appropriate period of incubation,the difference between intact or degraded femA in the presence and inthe absence of the test ligand is determined. The addition of a testligand which binds the folded femA, thus stabilizing it in theprotease-resistant form, changes the rate of proteolysis. A wide varietyof known proteases, such as trypsin, chymotrypsin, V8 protease,elastase, carboxypeptidase, proteinase K, thermolysin and subtilisin,can be used. As another example, the binding of the test ligand to thefemA is assessed through the use of antibodies that specifically bind tothe protein only in the unfolded state. There are numerous methods knownin the art for producing antibodies to a particular protein.

[0080] D. In vivo Assays to Evaluate Inhibitors

[0081] The inhibitors/binders identified by the initial screens such asthe one described in section C above are evaluated for their effect onvirulence in mouse models of S. aureus infection. Mice are infected with100 LD₅₀'s of the wild-type bacteria and treated with varying doses ofthe test inhibitory agent. The ED₅₀ of the test inhibitory agent (theamount in milligrams of drug per kilogram of body weight required tocure 50% of infected animals) is determined. Each trial contains twocontrol groups, a negative control group given an LD₅₀ dose of bacteriaand no test inhibitory agent (which ensures that the infecting dose isoperationally close to 100 LD₅₀'s), and a positive control group given a100 LD₅₀ dose of bacteria and an ED₅₀ dose of a known and effectiveantibiotic for that bacterial infection (which confirms that theinfection can be cured under appropriate conditions).

[0082] Five dosage levels of test inhibitory agent, utilizing serialtwo-fold dilutions, are employed. An exemplary dosage scheme begins witha minimum of 6.25 mg of drug per kg of body weight per dose, and rangesup to a maximum dose of 100 mg/kg. Each dosage level is tested in sixmice and deaths following infection and treatment are monitored for atleast 6 days. At the end of the test period, probit analysis is employedto determine the ED₅₀ value of the inhibitor or the amount of drug inmilligrams of drug per kilogram of body weight required to reversevirulence so as to result in only 50% mortality of infected animals inthe test. In tests designed to detect the prevention of infection byinterference with virulence factors, the first dose of the inhibitor isgiven one hour prior to infection, and then four and eight hours postinfection. In tests designed to detect virulence inhibitors whichreverse the outcome of infection, the inhibitor is administered one andfour hours post infection on the first day of infection and once in themorning and afternoon (separated by 6-8 hours) on the second and thirddays following infection. Inhibitory agents that successfully preventthe establishment of an infection or reverse the outcome of an infectiononce established are identified.

[0083] Numerous modifications and variations of the above-describedinvention are expected to occur to those of skill in the art.Accordingly, only such limitations as appear in the appended claimsshould be placed thereon.

1 106 1260 base pairs nucleic acid single linear other nucleic acid/desc = “DNA (genomic) (p2c73)” CDS 1..1257 1 ATG AAA TTT ACA GAG TTAACT GTT ACC GAA TTT GAC AAC TTT GTA CAA 48 Met Lys Phe Thr Glu Leu ThrVal Thr Glu Phe Asp Asn Phe Val Gln 1 5 10 15 AAT CCA TCA TTG GAA AGTCAT TAT TTC CAA GTA AAA GAA AAT ATA GTT 96 Asn Pro Ser Leu Glu Ser HisTyr Phe Gln Val Lys Glu Asn Ile Val 20 25 30 ACC CGT GAG AAT GAT GGC TTTGAA GTA GTT TTA TTA GGT ATT AAA GAC 144 Thr Arg Glu Asn Asp Gly Phe GluVal Val Leu Leu Gly Ile Lys Asp 35 40 45 GAC AAT AAC AAA GTA ATT GCA GCAAGC CTT TTC TCT AAA ATT CCT ACT 192 Asp Asn Asn Lys Val Ile Ala Ala SerLeu Phe Ser Lys Ile Pro Thr 50 55 60 ATG GGA AGT TAT GTT TAC TAT TCG AATCGT GGT CCA GTA ATG GAT TTT 240 Met Gly Ser Tyr Val Tyr Tyr Ser Asn ArgGly Pro Val Met Asp Phe 65 70 75 80 TCA GAT TTA GGA TTA GTT GAT TAT TATTTA AAA GAG TTA GAT AAA TAT 288 Ser Asp Leu Gly Leu Val Asp Tyr Tyr LeuLys Glu Leu Asp Lys Tyr 85 90 95 TTA CAG CAA CAT CAA TGT TTA TAT GTT AAATTA GAT CCG TAT TGG TTA 336 Leu Gln Gln His Gln Cys Leu Tyr Val Lys LeuAsp Pro Tyr Trp Leu 100 105 110 TAT CAT CTA TAT GAT AAA GAT ATC GTG CCATTT GAA GGT CGC GAG AAA 384 Tyr His Leu Tyr Asp Lys Asp Ile Val Pro PheGlu Gly Arg Glu Lys 115 120 125 AAT GAT GCC CTA GTA AAC TTG TTT AAA TCACAT GGT TAC GAG CAT CAT 432 Asn Asp Ala Leu Val Asn Leu Phe Lys Ser HisGly Tyr Glu His His 130 135 140 GGC TTT ACA ACT GAG TAT GAT ACA TCG AGCCAA GTA CGA TGG ATG GGC 480 Gly Phe Thr Thr Glu Tyr Asp Thr Ser Ser GlnVal Arg Trp Met Gly 145 150 155 160 GTA TTA AAC CTT GAA GGT AAA ACA CCCGAA ACA TTG AAA AAG ACA TTT 528 Val Leu Asn Leu Glu Gly Lys Thr Pro GluThr Leu Lys Lys Thr Phe 165 170 175 GAT AGT CAA CGT AAA CGT AAT ATT AATAAA GCG ATA AAC TAT GGT GTT 576 Asp Ser Gln Arg Lys Arg Asn Ile Asn LysAla Ile Asn Tyr Gly Val 180 185 190 AAA GTC AGA TTC CTT GAA CGT GAT GAGTTC AAT CTT TTC TTA GAT TTA 624 Lys Val Arg Phe Leu Glu Arg Asp Glu PheAsn Leu Phe Leu Asp Leu 195 200 205 TAT CGT GAA ACT GAA GAG CGT GCT GGATTT GTA TCA AAA ACA GAT GAT 672 Tyr Arg Glu Thr Glu Glu Arg Ala Gly PheVal Ser Lys Thr Asp Asp 210 215 220 TAT TTT TAT AAC TTT ATT GAC ACA TATGGA GAT AAA GTA TTA GTA CCA 720 Tyr Phe Tyr Asn Phe Ile Asp Thr Tyr GlyAsp Lys Val Leu Val Pro 225 230 235 240 TTA GCA TAT ATT GAC CTT GAT GAATAT GTG TTA AAG TTG CAA CAG GAA 768 Leu Ala Tyr Ile Asp Leu Asp Glu TyrVal Leu Lys Leu Gln Gln Glu 245 250 255 TTG AAT GAC AAA GAA AAT CGT CGTGAT CAA ATG ATG GCG AAA GAA AAC 816 Leu Asn Asp Lys Glu Asn Arg Arg AspGln Met Met Ala Lys Glu Asn 260 265 270 AAA TCA GAT AAG CAA ATG AAG AAAATT GCA GAA TTA GAT AAG CAA ATT 864 Lys Ser Asp Lys Gln Met Lys Lys IleAla Glu Leu Asp Lys Gln Ile 275 280 285 GAT CAT GAT CAG CAT GAA TTA TTGAAT GCA AGT GAA TTG AGC AAA ACG 912 Asp His Asp Gln His Glu Leu Leu AsnAla Ser Glu Leu Ser Lys Thr 290 295 300 GAC GGC CCA ATT CTA AAC CTT GCTTCT GGC GTT TAT TTT GCA AAT GCA 960 Asp Gly Pro Ile Leu Asn Leu Ala SerGly Val Tyr Phe Ala Asn Ala 305 310 315 320 TAT GAA GTG AAT TAT TTC TCTGGT GGT TCA TCA GAA AAA TAT AAT CAA 1008 Tyr Glu Val Asn Tyr Phe Ser GlyGly Ser Ser Glu Lys Tyr Asn Gln 325 330 335 TTT ATG GGA CCA TAC ATG ATGCAT TGG TTT ATG ATT AAC TAT TGC TTC 1056 Phe Met Gly Pro Tyr Met Met HisTrp Phe Met Ile Asn Tyr Cys Phe 340 345 350 GAT AAT GGC TAT GAT CGT TATAAT TTC TAT GGT TTA TCA GGT GAT TTT 1104 Asp Asn Gly Tyr Asp Arg Tyr AsnPhe Tyr Gly Leu Ser Gly Asp Phe 355 360 365 ACG GAA AAC AGT GAA GAT TATGGC GTA TAC CGC TTT AAA CGT GGA TTT 1152 Thr Glu Asn Ser Glu Asp Tyr GlyVal Tyr Arg Phe Lys Arg Gly Phe 370 375 380 AAT GTA CAA ATC GAA GAA TTAATA GGG GAT TTC TAT AAA CCA ATT CAT 1200 Asn Val Gln Ile Glu Glu Leu IleGly Asp Phe Tyr Lys Pro Ile His 385 390 395 400 AAA GTG AAA TAT TGG TTGTTC ACA ACA TTG GAT AAA TTA CGT AAA AAA 1248 Lys Val Lys Tyr Trp Leu PheThr Thr Leu Asp Lys Leu Arg Lys Lys 405 410 415 TTA AAG AAA TAG 1260 LeuLys Lys 419 amino acids amino acid linear protein 2 Met Lys Phe Thr GluLeu Thr Val Thr Glu Phe Asp Asn Phe Val Gln 1 5 10 15 Asn Pro Ser LeuGlu Ser His Tyr Phe Gln Val Lys Glu Asn Ile Val 20 25 30 Thr Arg Glu AsnAsp Gly Phe Glu Val Val Leu Leu Gly Ile Lys Asp 35 40 45 Asp Asn Asn LysVal Ile Ala Ala Ser Leu Phe Ser Lys Ile Pro Thr 50 55 60 Met Gly Ser TyrVal Tyr Tyr Ser Asn Arg Gly Pro Val Met Asp Phe 65 70 75 80 Ser Asp LeuGly Leu Val Asp Tyr Tyr Leu Lys Glu Leu Asp Lys Tyr 85 90 95 Leu Gln GlnHis Gln Cys Leu Tyr Val Lys Leu Asp Pro Tyr Trp Leu 100 105 110 Tyr HisLeu Tyr Asp Lys Asp Ile Val Pro Phe Glu Gly Arg Glu Lys 115 120 125 AsnAsp Ala Leu Val Asn Leu Phe Lys Ser His Gly Tyr Glu His His 130 135 140Gly Phe Thr Thr Glu Tyr Asp Thr Ser Ser Gln Val Arg Trp Met Gly 145 150155 160 Val Leu Asn Leu Glu Gly Lys Thr Pro Glu Thr Leu Lys Lys Thr Phe165 170 175 Asp Ser Gln Arg Lys Arg Asn Ile Asn Lys Ala Ile Asn Tyr GlyVal 180 185 190 Lys Val Arg Phe Leu Glu Arg Asp Glu Phe Asn Leu Phe LeuAsp Leu 195 200 205 Tyr Arg Glu Thr Glu Glu Arg Ala Gly Phe Val Ser LysThr Asp Asp 210 215 220 Tyr Phe Tyr Asn Phe Ile Asp Thr Tyr Gly Asp LysVal Leu Val Pro 225 230 235 240 Leu Ala Tyr Ile Asp Leu Asp Glu Tyr ValLeu Lys Leu Gln Gln Glu 245 250 255 Leu Asn Asp Lys Glu Asn Arg Arg AspGln Met Met Ala Lys Glu Asn 260 265 270 Lys Ser Asp Lys Gln Met Lys LysIle Ala Glu Leu Asp Lys Gln Ile 275 280 285 Asp His Asp Gln His Glu LeuLeu Asn Ala Ser Glu Leu Ser Lys Thr 290 295 300 Asp Gly Pro Ile Leu AsnLeu Ala Ser Gly Val Tyr Phe Ala Asn Ala 305 310 315 320 Tyr Glu Val AsnTyr Phe Ser Gly Gly Ser Ser Glu Lys Tyr Asn Gln 325 330 335 Phe Met GlyPro Tyr Met Met His Trp Phe Met Ile Asn Tyr Cys Phe 340 345 350 Asp AsnGly Tyr Asp Arg Tyr Asn Phe Tyr Gly Leu Ser Gly Asp Phe 355 360 365 ThrGlu Asn Ser Glu Asp Tyr Gly Val Tyr Arg Phe Lys Arg Gly Phe 370 375 380Asn Val Gln Ile Glu Glu Leu Ile Gly Asp Phe Tyr Lys Pro Ile His 385 390395 400 Lys Val Lys Tyr Trp Leu Phe Thr Thr Leu Asp Lys Leu Arg Lys Lys405 410 415 Leu Lys Lys 431 base pairs nucleic acid single linear othernucleic acid /desc = “DNA (genomic) (p2c90)” 3 GACGTGCTGA TGCAAATATAAGTNCATGAC CATCATGTTG TAATTGTNAC AATGTATCAA 60 TAATAGTCTG GTCAATTAATCGGCCGTCAA AACAAAGCGT ACCATCAATA TCANAGACAA 120 ATCTCATCAN ATCACTCCAAACAATATAAT ACCATGATTA TAGCATAAGT NAGTCATGTN 180 ACGATATTAG ATAATGATTATNATGTAGGG TACCTTTTGC CTTACACACA TATNACTTCC 240 TATNATATAT ATTATGTCAACNNGAATGTN AAATTCCATA AGGGGACTTA TATAACTGTN 300 TGTCTGTNTA GTGTTTATGTCAGTCAGCTA AATTNACATT CATGTTATGT CTCATTAAAC 360 CAATTACTCA CGTNTTGGTGCATATCNCAT CTTTCATATC GTCATACATC TATCCTCATT 420 CTCNTGNCTG A 431 729base pairs nucleic acid single linear other nucleic acid /desc = “DNA(genomic) (p9b74)” CDS 1..726 4 ATG ACT AAA TTA TTT ATA CCT TAT ATT ATGGGC AAT AAA GAT TTG ATT 48 Met Thr Lys Leu Phe Ile Pro Tyr Ile Met GlyAsn Lys Asp Leu Ile 1 5 10 15 GAA AAT GCA ACA TTG TTG AGT GAA AAT GGTGCA GAT ATA ATT GAA ATT 96 Glu Asn Ala Thr Leu Leu Ser Glu Asn Gly AlaAsp Ile Ile Glu Ile 20 25 30 GGA GTA CCT TTC TCT GAT CCG GTT GCT GAT GGTCCA GTT ATC ATG GAA 144 Gly Val Pro Phe Ser Asp Pro Val Ala Asp Gly ProVal Ile Met Glu 35 40 45 GCA GGT CAA CAA GCG ATT AAA CAA GGC ATC ACG ATAGAT TAT ATT TTC 192 Ala Gly Gln Gln Ala Ile Lys Gln Gly Ile Thr Ile AspTyr Ile Phe 50 55 60 AAT CAA TTA GAA AAA CAT GGT GAT CAA ATT AAG TGT AACTAT GTA TTA 240 Asn Gln Leu Glu Lys His Gly Asp Gln Ile Lys Cys Asn TyrVal Leu 65 70 75 80 ATG ACG TAT TAT AAT ATT ATT TGT CAT TAT GGA GAA CAAGCG TTT TTT 288 Met Thr Tyr Tyr Asn Ile Ile Cys His Tyr Gly Glu Gln AlaPhe Phe 85 90 95 GAA AAA TGT CGA GAT ACT GGT GTC TAC GGC TTA ATT ATT CCTGAT TTA 336 Glu Lys Cys Arg Asp Thr Gly Val Tyr Gly Leu Ile Ile Pro AspLeu 100 105 110 CCA TAT GAA TTA TCG CAG CGT TTA AAA CAA CAA TTT AGT CACTAT GGC 384 Pro Tyr Glu Leu Ser Gln Arg Leu Lys Gln Gln Phe Ser His TyrGly 115 120 125 GTC AAA ATC ATA TCG TTA GTT GCG ATG ACT ACT GAT GAC AAACGT ATA 432 Val Lys Ile Ile Ser Leu Val Ala Met Thr Thr Asp Asp Lys ArgIle 130 135 140 AAA GAT ATC GTA TCC CAT GCG GAA GGC TTT ATT TAT ACT GTGACG ATG 480 Lys Asp Ile Val Ser His Ala Glu Gly Phe Ile Tyr Thr Val ThrMet 145 150 155 160 AAT GCG ACA ACA GGG CAA AAC GGT GCG TTT CAT CCA GAATTA AAA CGA 528 Asn Ala Thr Thr Gly Gln Asn Gly Ala Phe His Pro Glu LeuLys Arg 165 170 175 AAA ATT GAG TCA ATT AAA GCG ATA GCC AAT GTG CCA GTTGTC GCA GGA 576 Lys Ile Glu Ser Ile Lys Ala Ile Ala Asn Val Pro Val ValAla Gly 180 185 190 TTT GGT ATA AGA ACA CCA CAA CAT GTT GCA GAT ATA AAAGAG GTT GCA 624 Phe Gly Ile Arg Thr Pro Gln His Val Ala Asp Ile Lys GluVal Ala 195 200 205 GAT GGC ATT GTC ATT GGT AGC GAA ATC GTT AAG CGA TTTAAA TCT AAC 672 Asp Gly Ile Val Ile Gly Ser Glu Ile Val Lys Arg Phe LysSer Asn 210 215 220 ACG CGT GAG GAA ATC ATT AAA TAT TTA CAA TCT ATC CAACAA ACA TTG 720 Thr Arg Glu Glu Ile Ile Lys Tyr Leu Gln Ser Ile Gln GlnThr Leu 225 230 235 240 AAT AAT TAA 729 Asn Asn 242 amino acids aminoacid linear protein 5 Met Thr Lys Leu Phe Ile Pro Tyr Ile Met Gly AsnLys Asp Leu Ile 1 5 10 15 Glu Asn Ala Thr Leu Leu Ser Glu Asn Gly AlaAsp Ile Ile Glu Ile 20 25 30 Gly Val Pro Phe Ser Asp Pro Val Ala Asp GlyPro Val Ile Met Glu 35 40 45 Ala Gly Gln Gln Ala Ile Lys Gln Gly Ile ThrIle Asp Tyr Ile Phe 50 55 60 Asn Gln Leu Glu Lys His Gly Asp Gln Ile LysCys Asn Tyr Val Leu 65 70 75 80 Met Thr Tyr Tyr Asn Ile Ile Cys His TyrGly Glu Gln Ala Phe Phe 85 90 95 Glu Lys Cys Arg Asp Thr Gly Val Tyr GlyLeu Ile Ile Pro Asp Leu 100 105 110 Pro Tyr Glu Leu Ser Gln Arg Leu LysGln Gln Phe Ser His Tyr Gly 115 120 125 Val Lys Ile Ile Ser Leu Val AlaMet Thr Thr Asp Asp Lys Arg Ile 130 135 140 Lys Asp Ile Val Ser His AlaGlu Gly Phe Ile Tyr Thr Val Thr Met 145 150 155 160 Asn Ala Thr Thr GlyGln Asn Gly Ala Phe His Pro Glu Leu Lys Arg 165 170 175 Lys Ile Glu SerIle Lys Ala Ile Ala Asn Val Pro Val Val Ala Gly 180 185 190 Phe Gly IleArg Thr Pro Gln His Val Ala Asp Ile Lys Glu Val Ala 195 200 205 Asp GlyIle Val Ile Gly Ser Glu Ile Val Lys Arg Phe Lys Ser Asn 210 215 220 ThrArg Glu Glu Ile Ile Lys Tyr Leu Gln Ser Ile Gln Gln Thr Leu 225 230 235240 Asn Asn 1260 base pairs nucleic acid single linear other nucleicacid /desc = “DNA (genomic) (p11c29/p13c83)” CDS 1..1257 6 ATG AAA TTTACA GAG TTA ACT GTT ACC GAA TTT GAC AAC TTT GTA CAA 48 Met Lys Phe ThrGlu Leu Thr Val Thr Glu Phe Asp Asn Phe Val Gln 1 5 10 15 AAT CCA TCATTG GAA AGT CAT TAT TTC CAA GTA AAA GAA AAT ATA GTT 96 Asn Pro Ser LeuGlu Ser His Tyr Phe Gln Val Lys Glu Asn Ile Val 20 25 30 ACC CGT GAG AATGAT GGC TTT GAA GTA GTT TTA TTA GGT ATT AAA GAC 144 Thr Arg Glu Asn AspGly Phe Glu Val Val Leu Leu Gly Ile Lys Asp 35 40 45 GAC AAT AAC AAA GTAATT GCA GCA AGC CTT TTC TCT AAA ATT CCT ACT 192 Asp Asn Asn Lys Val IleAla Ala Ser Leu Phe Ser Lys Ile Pro Thr 50 55 60 ATG GGA AGT TAT GTT TACTAT TCG AAT CGT GGT CCA GTA ATG GAT TTT 240 Met Gly Ser Tyr Val Tyr TyrSer Asn Arg Gly Pro Val Met Asp Phe 65 70 75 80 TCA GAT TTA GGA TTA GTTGAT TAT TAT TTA AAA GAG TTA GAT AAA TAT 288 Ser Asp Leu Gly Leu Val AspTyr Tyr Leu Lys Glu Leu Asp Lys Tyr 85 90 95 TTA CAG CAA CAT CAA TGT TTATAT GTT AAA TTA GAT CCG TAT TGG TTA 336 Leu Gln Gln His Gln Cys Leu TyrVal Lys Leu Asp Pro Tyr Trp Leu 100 105 110 TAT CAT CTA TAT GAT AAA GATATC GTG CCA TTT GAA GGT CGC GAG AAA 384 Tyr His Leu Tyr Asp Lys Asp IleVal Pro Phe Glu Gly Arg Glu Lys 115 120 125 AAT GAT GCC CTA GTA AAC TTGTTT AAA TCA CAT GGT TAC GAG CAT CAT 432 Asn Asp Ala Leu Val Asn Leu PheLys Ser His Gly Tyr Glu His His 130 135 140 GGC TTT ACA ACT GAG TAT GATACA TCG AGC CAA GTA CGA TGG ATG GGC 480 Gly Phe Thr Thr Glu Tyr Asp ThrSer Ser Gln Val Arg Trp Met Gly 145 150 155 160 GTA TTA AAC CTT GAA GGTAAA ACA CCC GAA ACA TTG AAA AAG ACA TTT 528 Val Leu Asn Leu Glu Gly LysThr Pro Glu Thr Leu Lys Lys Thr Phe 165 170 175 GAT AGT CAA CGT AAA CGTAAT ATT AAT AAA GCG ATA AAC TAT GGT GTT 576 Asp Ser Gln Arg Lys Arg AsnIle Asn Lys Ala Ile Asn Tyr Gly Val 180 185 190 AAA GTC AGA TTC CTT GAACGT GAT GAG TTC AAT CTT TTC TTA GAT TTA 624 Lys Val Arg Phe Leu Glu ArgAsp Glu Phe Asn Leu Phe Leu Asp Leu 195 200 205 TAT CGT GAA ACT GAA GAGCGT GCT GGA TTT GTA TCA AAA ACA GAT GAT 672 Tyr Arg Glu Thr Glu Glu ArgAla Gly Phe Val Ser Lys Thr Asp Asp 210 215 220 TAT TTT TAT AAC TTT ATTGAC ACA TAT GGA GAT AAA GTA TTA GTA CCA 720 Tyr Phe Tyr Asn Phe Ile AspThr Tyr Gly Asp Lys Val Leu Val Pro 225 230 235 240 TTA GCA TAT ATT GACCTT GAT GAA TAT GTG TTA AAG TTG CAA CAG GAA 768 Leu Ala Tyr Ile Asp LeuAsp Glu Tyr Val Leu Lys Leu Gln Gln Glu 245 250 255 TTG AAT GAC AAA GAAAAT CGT CGT GAT CAA ATG ATG GCG AAA GAA AAC 816 Leu Asn Asp Lys Glu AsnArg Arg Asp Gln Met Met Ala Lys Glu Asn 260 265 270 AAA TCA GAT AAG CAAATG AAG AAA ATT GCA GAA TTA GAT AAG CAA ATT 864 Lys Ser Asp Lys Gln MetLys Lys Ile Ala Glu Leu Asp Lys Gln Ile 275 280 285 GAT CAT GAT CAG CATGAA TTA TTG AAT GCA AGT GAA TTG AGC AAA ACG 912 Asp His Asp Gln His GluLeu Leu Asn Ala Ser Glu Leu Ser Lys Thr 290 295 300 GAC GGC CCA ATT CTAAAC CTT GCT TCT GGC GTT TAT TTT GCA AAT GCA 960 Asp Gly Pro Ile Leu AsnLeu Ala Ser Gly Val Tyr Phe Ala Asn Ala 305 310 315 320 TAT GAA GTG AATTAT TTC TCT GGT GGT TCA TCA GAA AAA TAT AAT CAA 1008 Tyr Glu Val Asn TyrPhe Ser Gly Gly Ser Ser Glu Lys Tyr Asn Gln 325 330 335 TTT ATG GGA CCATAC ATG ATG CAT TGG TTT ATG ATT AAC TAT TGC TTC 1056 Phe Met Gly Pro TyrMet Met His Trp Phe Met Ile Asn Tyr Cys Phe 340 345 350 GAT AAT GGC TATGAT CGT TAT AAT TTC TAT GGT TTA TCA GGT GAT TTT 1104 Asp Asn Gly Tyr AspArg Tyr Asn Phe Tyr Gly Leu Ser Gly Asp Phe 355 360 365 ACG GAA AAC AGTGAA GAT TAT GGC GTA TAC CGC TTT AAA CGT GGA TTT 1152 Thr Glu Asn Ser GluAsp Tyr Gly Val Tyr Arg Phe Lys Arg Gly Phe 370 375 380 AAT GTA CAA ATCGAA GAA TTA ATA GGG GAT TTC TAT AAA CCA ATT CAT 1200 Asn Val Gln Ile GluGlu Leu Ile Gly Asp Phe Tyr Lys Pro Ile His 385 390 395 400 AAA GTG AAATAT TGG TTG TTC ACA ACA TTG GAT AAA TTA CGT AAA AAA 1248 Lys Val Lys TyrTrp Leu Phe Thr Thr Leu Asp Lys Leu Arg Lys Lys 405 410 415 TTA AAG AAATAG 1260 Leu Lys Lys 419 amino acids amino acid linear protein 7 Met LysPhe Thr Glu Leu Thr Val Thr Glu Phe Asp Asn Phe Val Gln 1 5 10 15 AsnPro Ser Leu Glu Ser His Tyr Phe Gln Val Lys Glu Asn Ile Val 20 25 30 ThrArg Glu Asn Asp Gly Phe Glu Val Val Leu Leu Gly Ile Lys Asp 35 40 45 AspAsn Asn Lys Val Ile Ala Ala Ser Leu Phe Ser Lys Ile Pro Thr 50 55 60 MetGly Ser Tyr Val Tyr Tyr Ser Asn Arg Gly Pro Val Met Asp Phe 65 70 75 80Ser Asp Leu Gly Leu Val Asp Tyr Tyr Leu Lys Glu Leu Asp Lys Tyr 85 90 95Leu Gln Gln His Gln Cys Leu Tyr Val Lys Leu Asp Pro Tyr Trp Leu 100 105110 Tyr His Leu Tyr Asp Lys Asp Ile Val Pro Phe Glu Gly Arg Glu Lys 115120 125 Asn Asp Ala Leu Val Asn Leu Phe Lys Ser His Gly Tyr Glu His His130 135 140 Gly Phe Thr Thr Glu Tyr Asp Thr Ser Ser Gln Val Arg Trp MetGly 145 150 155 160 Val Leu Asn Leu Glu Gly Lys Thr Pro Glu Thr Leu LysLys Thr Phe 165 170 175 Asp Ser Gln Arg Lys Arg Asn Ile Asn Lys Ala IleAsn Tyr Gly Val 180 185 190 Lys Val Arg Phe Leu Glu Arg Asp Glu Phe AsnLeu Phe Leu Asp Leu 195 200 205 Tyr Arg Glu Thr Glu Glu Arg Ala Gly PheVal Ser Lys Thr Asp Asp 210 215 220 Tyr Phe Tyr Asn Phe Ile Asp Thr TyrGly Asp Lys Val Leu Val Pro 225 230 235 240 Leu Ala Tyr Ile Asp Leu AspGlu Tyr Val Leu Lys Leu Gln Gln Glu 245 250 255 Leu Asn Asp Lys Glu AsnArg Arg Asp Gln Met Met Ala Lys Glu Asn 260 265 270 Lys Ser Asp Lys GlnMet Lys Lys Ile Ala Glu Leu Asp Lys Gln Ile 275 280 285 Asp His Asp GlnHis Glu Leu Leu Asn Ala Ser Glu Leu Ser Lys Thr 290 295 300 Asp Gly ProIle Leu Asn Leu Ala Ser Gly Val Tyr Phe Ala Asn Ala 305 310 315 320 TyrGlu Val Asn Tyr Phe Ser Gly Gly Ser Ser Glu Lys Tyr Asn Gln 325 330 335Phe Met Gly Pro Tyr Met Met His Trp Phe Met Ile Asn Tyr Cys Phe 340 345350 Asp Asn Gly Tyr Asp Arg Tyr Asn Phe Tyr Gly Leu Ser Gly Asp Phe 355360 365 Thr Glu Asn Ser Glu Asp Tyr Gly Val Tyr Arg Phe Lys Arg Gly Phe370 375 380 Asn Val Gln Ile Glu Glu Leu Ile Gly Asp Phe Tyr Lys Pro IleHis 385 390 395 400 Lys Val Lys Tyr Trp Leu Phe Thr Thr Leu Asp Lys LeuArg Lys Lys 405 410 415 Leu Lys Lys 731 base pairs nucleic acid singlelinear other nucleic acid /desc = “DNA (genomic) CDS 1..417 CDS 524..7308 ATG GAA TTC ACT TAT TCG TAT TTA TTT AGA ATG ATT AGT CAT GAG ATG 48 MetGlu Phe Thr Tyr Ser Tyr Leu Phe Arg Met Ile Ser His Glu Met 1 5 10 15AAA CAA AAG GCT GAT CAA AAG TTA GAG CAA TTT GAT ATT ACA AAT GAG 96 LysGln Lys Ala Asp Gln Lys Leu Glu Gln Phe Asp Ile Thr Asn Glu 20 25 30 CAAGGT CAT ACG TTA GGT TAT CTT TAT GCA CAT CAA CAA GAT GGA CTG 144 Gln GlyHis Thr Leu Gly Tyr Leu Tyr Ala His Gln Gln Asp Gly Leu 35 40 45 ACA CAAAAT GAT ATT GCT AAA GCA TTA CAA CGA ACA GGT CCA ACT GTC 192 Thr Gln AsnAsp Ile Ala Lys Ala Leu Gln Arg Thr Gly Pro Thr Val 50 55 60 AGT AAT TTATTA AGG AAC CTT GAA CGT AAA AAG CTG ATC TAT CGC TAT 240 Ser Asn Leu LeuArg Asn Leu Glu Arg Lys Lys Leu Ile Tyr Arg Tyr 65 70 75 80 GTC GAT GCACAA GAT ACG AGA AGA AAG AAT ATA GGG CTG ACT ACC TCT 288 Val Asp Ala GlnAsp Thr Arg Arg Lys Asn Ile Gly Leu Thr Thr Ser 85 90 95 GGG ATT AAA CTCGTA GAA GCA TTC ACT TCG ATA TTT GAT GAA ATG GAA 336 Gly Ile Lys Leu ValGlu Ala Phe Thr Ser Ile Phe Asp Glu Met Glu 100 105 110 CAA ACA CTC GTATCG CAG TTA TCT GAA GAA GAA AAT GAA CAA ATG AAA 384 Gln Thr Leu Val SerGln Leu Ser Glu Glu Glu Asn Glu Gln Met Lys 115 120 125 GCA AAC TTA ACTAAA ATG TTA TCT AGT TTA CAA TAAATGATAA GTGTGACTGG 437 Ala Asn Leu ThrLys Met Leu Ser Ser Leu Gln 130 135 TAGAAATCAG TCACTTTGTC TTTAATATTATAGTTAGATA TCTAATTGTT AGTAAGCTAA 497 TTATTGGAAA AGACAAGGAG TATTGA ACAATG AAA GAC GAA CAA TTA TAT TAT 550 Thr Met Lys Asp Glu Gln Leu Tyr Tyr1 5 TTT GAG AAA TCG CCA GTA TTT AAA GCG ATG ATG CAT TTC TCA TTG CCA 598Phe Glu Lys Ser Pro Val Phe Lys Ala Met Met His Phe Ser Leu Pro 10 15 2025 ATG ATG ATA GGG ACT TTA TTA AGC GTT ATT TAT GGC ATA TTA AAT ATT 646Met Met Ile Gly Thr Leu Leu Ser Val Ile Tyr Gly Ile Leu Asn Ile 30 35 40TAC TTT ATA GGA TTT TYA GAM GAY AGC CAC ATG ATT TCT GCT AAT CTC 694 TyrPhe Ile Gly Phe Xaa Xaa Asp Ser His Met Ile Ser Ala Asn Leu 45 50 55 TCTAAC ACT GCC AGT ATT TGC TAT CTT AAT GGG GTT A 731 Ser Asn Thr Ala SerIle Cys Tyr Leu Asn Gly Val 60 65 139 amino acids amino acid linearprotein 9 Met Glu Phe Thr Tyr Ser Tyr Leu Phe Arg Met Ile Ser His GluMet 1 5 10 15 Lys Gln Lys Ala Asp Gln Lys Leu Glu Gln Phe Asp Ile ThrAsn Glu 20 25 30 Gln Gly His Thr Leu Gly Tyr Leu Tyr Ala His Gln Gln AspGly Leu 35 40 45 Thr Gln Asn Asp Ile Ala Lys Ala Leu Gln Arg Thr Gly ProThr Val 50 55 60 Ser Asn Leu Leu Arg Asn Leu Glu Arg Lys Lys Leu Ile TyrArg Tyr 65 70 75 80 Val Asp Ala Gln Asp Thr Arg Arg Lys Asn Ile Gly LeuThr Thr Ser 85 90 95 Gly Ile Lys Leu Val Glu Ala Phe Thr Ser Ile Phe AspGlu Met Glu 100 105 110 Gln Thr Leu Val Ser Gln Leu Ser Glu Glu Glu AsnGlu Gln Met Lys 115 120 125 Ala Asn Leu Thr Lys Met Leu Ser Ser Leu Gln130 135 69 amino acids amino acid linear protein 10 Thr Met Lys Asp GluGln Leu Tyr Tyr Phe Glu Lys Ser Pro Val Phe 1 5 10 15 Lys Ala Met MetHis Phe Ser Leu Pro Met Met Ile Gly Thr Leu Leu 20 25 30 Ser Val Ile TyrGly Ile Leu Asn Ile Tyr Phe Ile Gly Phe Xaa Xaa 35 40 45 Asp Ser His MetIle Ser Ala Asn Leu Ser Asn Thr Ala Ser Ile Cys 50 55 60 Tyr Leu Asn GlyVal 65 828 base pairs nucleic acid single linear other nucleic acid/desc = ”DNA (genomic) (p5c4)“ CDS 1..825 11 GTG AGG TAC TTG CCT GTG AATGCG ATT GAA ATC CCG ACT ACC GCC GGC 48 Val Arg Tyr Leu Pro Val Asn AlaIle Glu Ile Pro Thr Thr Ala Gly 1 5 10 15 ACG CCT GAC GCG CCC TTC TACCAA CCG TTG GGC AAT GAA GAG CAG CTG 96 Thr Pro Asp Ala Pro Phe Tyr GlnPro Leu Gly Asn Glu Glu Gln Leu 20 25 30 TTC CAG CAG GCC TGG CAG CAC GGCATG CCC GTG CTT ATC AAG GGC CCG 144 Phe Gln Gln Ala Trp Gln His Gly MetPro Val Leu Ile Lys Gly Pro 35 40 45 ACC GGC TGC GGC AAG ACC CGT TTC GTACAG CAC ATG GCG CAT CGC CTG 192 Thr Gly Cys Gly Lys Thr Arg Phe Val GlnHis Met Ala His Arg Leu 50 55 60 AAT CTG CCG CTG TAC ACC GTG GCC TGC CATGAC GAC CTG TCG GCG GCC 240 Asn Leu Pro Leu Tyr Thr Val Ala Cys His AspAsp Leu Ser Ala Ala 65 70 75 80 GAC CTG GTC GGC CGA CAC CTG ATC GGC GCACAG GGC ACC TGG TGG CAG 288 Asp Leu Val Gly Arg His Leu Ile Gly Ala GlnGly Thr Trp Trp Gln 85 90 95 GAC GGT CCG CTG ACC CGC GCG GTC CGC GAA GGAGGC ATC TGC TAC CTG 336 Asp Gly Pro Leu Thr Arg Ala Val Arg Glu Gly GlyIle Cys Tyr Leu 100 105 110 GAC GAA GTG GTG GAA GCA CGG CAG GAC ACC GCCGTG GTA CTG CAC CCG 384 Asp Glu Val Val Glu Ala Arg Gln Asp Thr Ala ValVal Leu His Pro 115 120 125 CTG GCC GAT GAT CGC CGC GAA CTG TTC ATC GAGCGC ACC GGC GAG GCG 432 Leu Ala Asp Asp Arg Arg Glu Leu Phe Ile Glu ArgThr Gly Glu Ala 130 135 140 CTC AAG GCG CCG CCG GGC TTC ATG CTG GTG GTGTCC TAC AAC CCC GGT 480 Leu Lys Ala Pro Pro Gly Phe Met Leu Val Val SerTyr Asn Pro Gly 145 150 155 160 TAC CAA AAC CTG CTC AAG GGC ATG AAG CCCAGC ACC CGC CAG CGC TTC 528 Tyr Gln Asn Leu Leu Lys Gly Met Lys Pro SerThr Arg Gln Arg Phe 165 170 175 GTG GCG ATG CGC TTC GAC TAT CCG CCG ACCGCC GAG GAA GAG CGC ATC 576 Val Ala Met Arg Phe Asp Tyr Pro Pro Thr AlaGlu Glu Glu Arg Ile 180 185 190 GTC GCC AAC GAG GCG CAG GTC GAT GCC GCGCTC GCC GCC CAG GTG GTC 624 Val Ala Asn Glu Ala Gln Val Asp Ala Ala LeuAla Ala Gln Val Val 195 200 205 AAG CTT GGC CAG GCA CTG CGT CGG CTG GAACAG CAC GAT CTG GAG GAA 672 Lys Leu Gly Gln Ala Leu Arg Arg Leu Glu GlnHis Asp Leu Glu Glu 210 215 220 GTC GCC TCG ACC CGC CTG CTG ATC TTC ACCGCA CGC ATG ATC CGC TCC 720 Val Ala Ser Thr Arg Leu Leu Ile Phe Thr AlaArg Met Ile Arg Ser 225 230 235 240 GGC ATG ACG CCG CGG CAG GCC TGC CTGGCC TGC CTC GCC GAA CCG CTG 768 Gly Met Thr Pro Arg Gln Ala Cys Leu AlaCys Leu Ala Glu Pro Leu 245 250 255 TCG GAT GAT CCG CAG ACC GTT GCC GCGCTG ATG GAT GTG GTC TAT GTC 816 Ser Asp Asp Pro Gln Thr Val Ala Ala LeuMet Asp Val Val Tyr Val 260 265 270 CAC TTC GGC TGA 828 His Phe Gly 275275 amino acids amino acid linear protein 12 Val Arg Tyr Leu Pro Val AsnAla Ile Glu Ile Pro Thr Thr Ala Gly 1 5 10 15 Thr Pro Asp Ala Pro PheTyr Gln Pro Leu Gly Asn Glu Glu Gln Leu 20 25 30 Phe Gln Gln Ala Trp GlnHis Gly Met Pro Val Leu Ile Lys Gly Pro 35 40 45 Thr Gly Cys Gly Lys ThrArg Phe Val Gln His Met Ala His Arg Leu 50 55 60 Asn Leu Pro Leu Tyr ThrVal Ala Cys His Asp Asp Leu Ser Ala Ala 65 70 75 80 Asp Leu Val Gly ArgHis Leu Ile Gly Ala Gln Gly Thr Trp Trp Gln 85 90 95 Asp Gly Pro Leu ThrArg Ala Val Arg Glu Gly Gly Ile Cys Tyr Leu 100 105 110 Asp Glu Val ValGlu Ala Arg Gln Asp Thr Ala Val Val Leu His Pro 115 120 125 Leu Ala AspAsp Arg Arg Glu Leu Phe Ile Glu Arg Thr Gly Glu Ala 130 135 140 Leu LysAla Pro Pro Gly Phe Met Leu Val Val Ser Tyr Asn Pro Gly 145 150 155 160Tyr Gln Asn Leu Leu Lys Gly Met Lys Pro Ser Thr Arg Gln Arg Phe 165 170175 Val Ala Met Arg Phe Asp Tyr Pro Pro Thr Ala Glu Glu Glu Arg Ile 180185 190 Val Ala Asn Glu Ala Gln Val Asp Ala Ala Leu Ala Ala Gln Val Val195 200 205 Lys Leu Gly Gln Ala Leu Arg Arg Leu Glu Gln His Asp Leu GluGlu 210 215 220 Val Ala Ser Thr Arg Leu Leu Ile Phe Thr Ala Arg Met IleArg Ser 225 230 235 240 Gly Met Thr Pro Arg Gln Ala Cys Leu Ala Cys LeuAla Glu Pro Leu 245 250 255 Ser Asp Asp Pro Gln Thr Val Ala Ala Leu MetAsp Val Val Tyr Val 260 265 270 His Phe Gly 275 528 base pairs nucleicacid single linear other nucleic acid /desc = ”DNA (genomic) (p9b66)“CDS 1..525 13 ATG GAA AGG ATG TCA AAA ATG AAT ATT AAT ACA GCT TAT TTTGCC GGA 48 Met Glu Arg Met Ser Lys Met Asn Ile Asn Thr Ala Tyr Phe AlaGly 1 5 10 15 GGT TGC TTT TGG TGT ATG ACG AAA CCA TTT GAC ACC TTT GACGGC ATA 96 Gly Cys Phe Trp Cys Met Thr Lys Pro Phe Asp Thr Phe Asp GlyIle 20 25 30 GAA AAA GTA ACT TCT GGA TAT ATG GGC GGA CAT ATT GAA AAT CCTACT 144 Glu Lys Val Thr Ser Gly Tyr Met Gly Gly His Ile Glu Asn Pro Thr35 40 45 TAC GAA CAA GTA AAA TCA GGT ACG AGT GGT CAT TTA GAA ACT GTT GAA192 Tyr Glu Gln Val Lys Ser Gly Thr Ser Gly His Leu Glu Thr Val Glu 5055 60 ATT CAA TAT GAT GTT GCA TTA TTC TCA TAC AAT AAG TTA TTA GAA ATA240 Ile Gln Tyr Asp Val Ala Leu Phe Ser Tyr Asn Lys Leu Leu Glu Ile 6570 75 80 TTT TTC TCA GTC ATT GAC CCA TTA GAT ACA GGT GGT CAA TAT CAA GAC288 Phe Phe Ser Val Ile Asp Pro Leu Asp Thr Gly Gly Gln Tyr Gln Asp 8590 95 CGT GGT CCT CAA TAT NAA ACA GCT ATT TTC TAC ACT AAT GAT CAT CAA336 Arg Gly Pro Gln Tyr Xaa Thr Ala Ile Phe Tyr Thr Asn Asp His Gln 100105 110 AAA GAA CTC GCT GAG ACT TAT ATC GAG CAG CTT AAA AAT ACG ATT AAT384 Lys Glu Leu Ala Glu Thr Tyr Ile Glu Gln Leu Lys Asn Thr Ile Asn 115120 125 GCT GAT AAG GCA ATT GCA ACA AAA ATA YTA CCA GCG TCA CAA TTT TAC432 Ala Asp Lys Ala Ile Ala Thr Lys Ile Leu Pro Ala Ser Gln Phe Tyr 130135 140 AAA GCC GAA GAC TAT CAC CAA GAT TTT TAT AAG AAA AAT CCA GAG CGC480 Lys Ala Glu Asp Tyr His Gln Asp Phe Tyr Lys Lys Asn Pro Glu Arg 145150 155 160 TAT GCA GAA GAA CAA AAA ATA CGC CAA GAA TAC AAA AAT AAG CAA525 Tyr Ala Glu Glu Gln Lys Ile Arg Gln Glu Tyr Lys Asn Lys Gln 165 170175 TAA 528 175 amino acids amino acid linear protein 14 Met Glu Arg MetSer Lys Met Asn Ile Asn Thr Ala Tyr Phe Ala Gly 1 5 10 15 Gly Cys PheTrp Cys Met Thr Lys Pro Phe Asp Thr Phe Asp Gly Ile 20 25 30 Glu Lys ValThr Ser Gly Tyr Met Gly Gly His Ile Glu Asn Pro Thr 35 40 45 Tyr Glu GlnVal Lys Ser Gly Thr Ser Gly His Leu Glu Thr Val Glu 50 55 60 Ile Gln TyrAsp Val Ala Leu Phe Ser Tyr Asn Lys Leu Leu Glu Ile 65 70 75 80 Phe PheSer Val Ile Asp Pro Leu Asp Thr Gly Gly Gln Tyr Gln Asp 85 90 95 Arg GlyPro Gln Tyr Xaa Thr Ala Ile Phe Tyr Thr Asn Asp His Gln 100 105 110 LysGlu Leu Ala Glu Thr Tyr Ile Glu Gln Leu Lys Asn Thr Ile Asn 115 120 125Ala Asp Lys Ala Ile Ala Thr Lys Ile Leu Pro Ala Ser Gln Phe Tyr 130 135140 Lys Ala Glu Asp Tyr His Gln Asp Phe Tyr Lys Lys Asn Pro Glu Arg 145150 155 160 Tyr Ala Glu Glu Gln Lys Ile Arg Gln Glu Tyr Lys Asn Lys Gln165 170 175 702 base pairs nucleic acid single linear other nucleic acid/desc = ”DNA (genomic) (p7c26)“ CDS 1..699 15 ATG ATT GAG TTA AAA CATGTG ACT TTT GGT TAT AAT AAA AAG CAG ATG 48 Met Ile Glu Leu Lys His ValThr Phe Gly Tyr Asn Lys Lys Gln Met 1 5 10 15 GTG CTA CAA GAT ATC AATATT ACT ATA CCT GAT GGA GAA AAT GTT GGT 96 Val Leu Gln Asp Ile Asn IleThr Ile Pro Asp Gly Glu Asn Val Gly 20 25 30 ATT TTA GGC GAA AGT GGC TGTGGT AAA AGT ACG CTC GCT TCA TTG GTT 144 Ile Leu Gly Glu Ser Gly Cys GlyLys Ser Thr Leu Ala Ser Leu Val 35 40 45 CTT GGC TTG TTT AAA CCT GTT AAAGGA GAG ATT TAC TTA AGT GAC AAT 192 Leu Gly Leu Phe Lys Pro Val Lys GlyGlu Ile Tyr Leu Ser Asp Asn 50 55 60 GCT GTG TTA CCG ATT TTC CAA CAC CCTTTA ACT AGC TTT AAC CCT GAT 240 Ala Val Leu Pro Ile Phe Gln His Pro LeuThr Ser Phe Asn Pro Asp 65 70 75 80 TGG ACG ATT GAG ACC TCA TTA AAA GAAGCG TTA TAT TAT TAC AGA GGT 288 Trp Thr Ile Glu Thr Ser Leu Lys Glu AlaLeu Tyr Tyr Tyr Arg Gly 85 90 95 CTA ACT GAT AAT ACT GCT CAG GAT CAA TTATTA TTA CAA CAT TTA TCT 336 Leu Thr Asp Asn Thr Ala Gln Asp Gln Leu LeuLeu Gln His Leu Ser 100 105 110 ACT TTT GAG TTA AAC GCG CAA TTA TTG ACTAAA TTA CCA AGC GAA GTG 384 Thr Phe Glu Leu Asn Ala Gln Leu Leu Thr LysLeu Pro Ser Glu Val 115 120 125 AGT GGC GGA CAA TTA CAA AGA TTT AAT GTCATG CGT TCG TTA TTA GCA 432 Ser Gly Gly Gln Leu Gln Arg Phe Asn Val MetArg Ser Leu Leu Ala 130 135 140 CAG CCT CGC GTT TTA ATA TGT GAT GAG ATAACT TCA AAT TTA GAT GTT 480 Gln Pro Arg Val Leu Ile Cys Asp Glu Ile ThrSer Asn Leu Asp Val 145 150 155 160 ATA GCT GAA CAA AAT GTA ATC AAT ATATTA AAA GCG CAA ACG ATT ACG 528 Ile Ala Glu Gln Asn Val Ile Asn Ile LeuLys Ala Gln Thr Ile Thr 165 170 175 AAC TTA AAT CAT TTT ATC GTT ATT TCTCAT GAT TTA TCC GTG TTA CAA 576 Asn Leu Asn His Phe Ile Val Ile Ser HisAsp Leu Ser Val Leu Gln 180 185 190 CGC TTA GTT AAT AGA ATT ATC GTT CTTAAG GAT GGC ATG ATA GTC GAT 624 Arg Leu Val Asn Arg Ile Ile Val Leu LysAsp Gly Met Ile Val Asp 195 200 205 GAT TTT GCA ATA GAG GAA TTA TTT AATGTT GAT AGA CAC CCT TAT ACA 672 Asp Phe Ala Ile Glu Glu Leu Phe Asn ValAsp Arg His Pro Tyr Thr 210 215 220 AAA GAA TTA GTG CAA GCA TTT TCA TATTAG 702 Lys Glu Leu Val Gln Ala Phe Ser Tyr 225 230 233 amino acidsamino acid linear protein 16 Met Ile Glu Leu Lys His Val Thr Phe Gly TyrAsn Lys Lys Gln Met 1 5 10 15 Val Leu Gln Asp Ile Asn Ile Thr Ile ProAsp Gly Glu Asn Val Gly 20 25 30 Ile Leu Gly Glu Ser Gly Cys Gly Lys SerThr Leu Ala Ser Leu Val 35 40 45 Leu Gly Leu Phe Lys Pro Val Lys Gly GluIle Tyr Leu Ser Asp Asn 50 55 60 Ala Val Leu Pro Ile Phe Gln His Pro LeuThr Ser Phe Asn Pro Asp 65 70 75 80 Trp Thr Ile Glu Thr Ser Leu Lys GluAla Leu Tyr Tyr Tyr Arg Gly 85 90 95 Leu Thr Asp Asn Thr Ala Gln Asp GlnLeu Leu Leu Gln His Leu Ser 100 105 110 Thr Phe Glu Leu Asn Ala Gln LeuLeu Thr Lys Leu Pro Ser Glu Val 115 120 125 Ser Gly Gly Gln Leu Gln ArgPhe Asn Val Met Arg Ser Leu Leu Ala 130 135 140 Gln Pro Arg Val Leu IleCys Asp Glu Ile Thr Ser Asn Leu Asp Val 145 150 155 160 Ile Ala Glu GlnAsn Val Ile Asn Ile Leu Lys Ala Gln Thr Ile Thr 165 170 175 Asn Leu AsnHis Phe Ile Val Ile Ser His Asp Leu Ser Val Leu Gln 180 185 190 Arg LeuVal Asn Arg Ile Ile Val Leu Lys Asp Gly Met Ile Val Asp 195 200 205 AspPhe Ala Ile Glu Glu Leu Phe Asn Val Asp Arg His Pro Tyr Thr 210 215 220Lys Glu Leu Val Gln Ala Phe Ser Tyr 225 230 1471 base pairs nucleic acidsingle linear other nucleic acid /desc = ”DNA (genomic) (p10c15)“ CDS1..774 17 ATG ATG AGT CTC ATT GAT ATA CAA AAT TTA ACA ATA AAG AAT ACTAGT 48 Met Met Ser Leu Ile Asp Ile Gln Asn Leu Thr Ile Lys Asn Thr Ser 15 10 15 GAG AAA TCT CTT ATT AAA GGG ATT GAT TTG AAA ATT TTT AGT CAA CAG96 Glu Lys Ser Leu Ile Lys Gly Ile Asp Leu Lys Ile Phe Ser Gln Gln 20 2530 ATT AAT GCC TTG ATT GGA GAG AGC GGC GCT GGA AAA AGT TTG ATT GCT 144Ile Asn Ala Leu Ile Gly Glu Ser Gly Ala Gly Lys Ser Leu Ile Ala 35 40 45AAA GCT TTA CTT GAA TAT TTA CCA TTT GAT TTA AGC TGC ACG TAT GAT 192 LysAla Leu Leu Glu Tyr Leu Pro Phe Asp Leu Ser Cys Thr Tyr Asp 50 55 60 TCGTAC CAA TTT GAT GGG GAA AAT GTT AGT AGA TTG AGT CAA TAT TAT 240 Ser TyrGln Phe Asp Gly Glu Asn Val Ser Arg Leu Ser Gln Tyr Tyr 65 70 75 80 GGTCAT ACA ATT GGC TAT ATT TCT CAA AAT TAT GCA GAA AGT TTT AAC 288 Gly HisThr Ile Gly Tyr Ile Ser Gln Asn Tyr Ala Glu Ser Phe Asn 85 90 95 GAC CATACT AAA TTA GGT AAA CAG TTA ACT GCG ATT TAT CGT AAG CAT 336 Asp His ThrLys Leu Gly Lys Gln Leu Thr Ala Ile Tyr Arg Lys His 100 105 110 TAT AAAGGT AGT AAA GAA GAG GCT TTG TCC AAA GTT GAT AAG GCT TTG 384 Tyr Lys GlySer Lys Glu Glu Ala Leu Ser Lys Val Asp Lys Ala Leu 115 120 125 TCG TGGGTT AAT TTA CAA AGC AAA GAT ATA TTA AAT AAA TAT AGT TTC 432 Ser Trp ValAsn Leu Gln Ser Lys Asp Ile Leu Asn Lys Tyr Ser Phe 130 135 140 CAA CTTTCT GGG GGC CAA CTT GAA CGC GTA TAC ATA GCA AGC GTT CTC 480 Gln Leu SerGly Gly Gln Leu Glu Arg Val Tyr Ile Ala Ser Val Leu 145 150 155 160 ATGTTG GAG CCT AAA TTA ATC ATT GCA GAC GAA CCA GTT GCA TCA TTG 528 Met LeuGlu Pro Lys Leu Ile Ile Ala Asp Glu Pro Val Ala Ser Leu 165 170 175 GATGCT TTG AAC GGT AAT CAA GTG ATG GAT TTA TTA CAG CAT ATT GTA 576 Asp AlaLeu Asn Gly Asn Gln Val Met Asp Leu Leu Gln His Ile Val 180 185 190 TTAGAA CAT GGT CAA ACA TTA TTT ATT ATC ACA CAT AAC TTA AGT CAT 624 Leu GluHis Gly Gln Thr Leu Phe Ile Ile Thr His Asn Leu Ser His 195 200 205 GTATTG AAA TAT TGT CAG TAC ATT TAT GTT TTA AAA GAA GGT CAA ATC 672 Val LeuLys Tyr Cys Gln Tyr Ile Tyr Val Leu Lys Glu Gly Gln Ile 210 215 220 ATTGAA CGA GGT AAT ATT AAT CAT TTC AAG TAT GAG CAT TTG CAT CCG 720 Ile GluArg Gly Asn Ile Asn His Phe Lys Tyr Glu His Leu His Pro 225 230 235 240TAT ACT GAA CGT CTA ATT AAA TAT AGA ACA CAA TTA AAG AGG GAT TAC 768 TyrThr Glu Arg Leu Ile Lys Tyr Arg Thr Gln Leu Lys Arg Asp Tyr 245 250 255TAT GAT TGAGTTAAAA CATGTGACTT TTGGTTATAA TAAAAAGCAG ATGGTGCTAC 824 TyrAsp AAGATATCAA TATTACTATA CCTGATGGAG AAAATGTTGG TATTTTAGGC GAAAGTGGCT884 GTGGTAAAAG TACGCTCGCT TCATTGGTTC TTGGCTTGTT TAAACCTGTT AAAGGAGAGA944 TTTACTTAAG TGACAATGCT GTGTTACCGA TTTTCCAACA CCCTTTAACT AGCTTTAACC1004 CTGATTGGAC GATTGAGACC TCATTAAAAG AAGCGTTATA TTATTACAGA GGTCTAACTG1064 ATAATACTGC TCAGGATCAA TTATTATTAC AACATTTATC TACTTTTGAG TTAAACGCGC1124 AATTATTGAC TAAATTACCA AGCGAAGTGA GTGGCGGACA ATTACAAAGA TTTAATGTCA1184 TGCGTTCGTT ATTAGCACAG CCTCGCGTTT TAATATGTGA TGAGATAACT TCAAATTTAG1244 ATGTTATAGC TGAACAAAAT GTAATCAATA TATTAAAAGC GCAAACGATT ACGAACTTAA1304 ATCATTTTAT CGTTATTTCT CATGATTTAT CCGTGTTACA ACGCTTAGTT AATAGAATTA1364 TCGTTCTTAA GGATGGCATG ATAGTCGATG ATTTTGCAAT AGAGGAATTA TTTAATGTTG1424 ATAGACACCC TTATACAAAA GAATTAGTGC AAGCATTTTC ATATTAG 1471 258 aminoacids amino acid linear protein 18 Met Met Ser Leu Ile Asp Ile Gln AsnLeu Thr Ile Lys Asn Thr Ser 1 5 10 15 Glu Lys Ser Leu Ile Lys Gly IleAsp Leu Lys Ile Phe Ser Gln Gln 20 25 30 Ile Asn Ala Leu Ile Gly Glu SerGly Ala Gly Lys Ser Leu Ile Ala 35 40 45 Lys Ala Leu Leu Glu Tyr Leu ProPhe Asp Leu Ser Cys Thr Tyr Asp 50 55 60 Ser Tyr Gln Phe Asp Gly Glu AsnVal Ser Arg Leu Ser Gln Tyr Tyr 65 70 75 80 Gly His Thr Ile Gly Tyr IleSer Gln Asn Tyr Ala Glu Ser Phe Asn 85 90 95 Asp His Thr Lys Leu Gly LysGln Leu Thr Ala Ile Tyr Arg Lys His 100 105 110 Tyr Lys Gly Ser Lys GluGlu Ala Leu Ser Lys Val Asp Lys Ala Leu 115 120 125 Ser Trp Val Asn LeuGln Ser Lys Asp Ile Leu Asn Lys Tyr Ser Phe 130 135 140 Gln Leu Ser GlyGly Gln Leu Glu Arg Val Tyr Ile Ala Ser Val Leu 145 150 155 160 Met LeuGlu Pro Lys Leu Ile Ile Ala Asp Glu Pro Val Ala Ser Leu 165 170 175 AspAla Leu Asn Gly Asn Gln Val Met Asp Leu Leu Gln His Ile Val 180 185 190Leu Glu His Gly Gln Thr Leu Phe Ile Ile Thr His Asn Leu Ser His 195 200205 Val Leu Lys Tyr Cys Gln Tyr Ile Tyr Val Leu Lys Glu Gly Gln Ile 210215 220 Ile Glu Arg Gly Asn Ile Asn His Phe Lys Tyr Glu His Leu His Pro225 230 235 240 Tyr Thr Glu Arg Leu Ile Lys Tyr Arg Thr Gln Leu Lys ArgAsp Tyr 245 250 255 Tyr Asp 1471 base pairs nucleic acid single linearother nucleic acid /desc = ”DNA (genomic) (p10c15)“ CDS 770..1468 19ATGATGAGTC TCATTGATAT ACAAAATTTA ACAATAAAGA ATACTAGTGA GAAATCTCTT 60ATTAAAGGGA TTGATTTGAA AATTTTTAGT CAACAGATTA ATGCCTTGAT TGGAGAGAGC 120GGCGCTGGAA AAAGTTTGAT TGCTAAAGCT TTACTTGAAT ATTTACCATT TGATTTAAGC 180TGCACGTATG ATTCGTACCA ATTTGATGGG GAAAATGTTA GTAGATTGAG TCAATATTAT 240GGTCATACAA TTGGCTATAT TTCTCAAAAT TATGCAGAAA GTTTTAACGA CCATACTAAA 300TTAGGTAAAC AGTTAACTGC GATTTATCGT AAGCATTATA AAGGTAGTAA AGAAGAGGCT 360TTGTCCAAAG TTGATAAGGC TTTGTCGTGG GTTAATTTAC AAAGCAAAGA TATATTAAAT 420AAATATAGTT TCCAACTTTC TGGGGGCCAA CTTGAACGCG TATACATAGC AAGCGTTCTC 480ATGTTGGAGC CTAAATTAAT CATTGCAGAC GAACCAGTTG CATCATTGGA TGCTTTGAAC 540GGTAATCAAG TGATGGATTT ATTACAGCAT ATTGTATTAG AACATGGTCA AACATTATTT 600ATTATCACAC ATAACTTAAG TCATGTATTG AAATATTGTC AGTACATTTA TGTTTTAAAA 660GAAGGTCAAA TCATTGAACG AGGTAATATT AATCATTTCA AGTATGAGCA TTTGCATCCG 720TATACTGAAC GTCTAATTAA ATATAGAACA CAATTAAAGA GGGATTACT ATG ATT 775 MetIle 1 GAG TTA AAA CAT GTG ACT TTT GGT TAT AAT AAA AAG CAG ATG GTG CTA823 Glu Leu Lys His Val Thr Phe Gly Tyr Asn Lys Lys Gln Met Val Leu 5 1015 CAA GAT ATC AAT ATT ACT ATA CCT GAT GGA GAA AAT GTT GGT ATT TTA 871Gln Asp Ile Asn Ile Thr Ile Pro Asp Gly Glu Asn Val Gly Ile Leu 20 25 30GGC GAA AGT GGC TGT GGT AAA AGT ACG CTC GCT TCA TTG GTT CTT GGC 919 GlyGlu Ser Gly Cys Gly Lys Ser Thr Leu Ala Ser Leu Val Leu Gly 35 40 45 50TTG TTT AAA CCT GTT AAA GGA GAG ATT TAC TTA AGT GAC AAT GCT GTG 967 LeuPhe Lys Pro Val Lys Gly Glu Ile Tyr Leu Ser Asp Asn Ala Val 55 60 65 TTACCG ATT TTC CAA CAC CCT TTA ACT AGC TTT AAC CCT GAT TGG ACG 1015 Leu ProIle Phe Gln His Pro Leu Thr Ser Phe Asn Pro Asp Trp Thr 70 75 80 ATT GAGACC TCA TTA AAA GAA GCG TTA TAT TAT TAC AGA GGT CTA ACT 1063 Ile Glu ThrSer Leu Lys Glu Ala Leu Tyr Tyr Tyr Arg Gly Leu Thr 85 90 95 GAT AAT ACTGCT CAG GAT CAA TTA TTA TTA CAA CAT TTA TCT ACT TTT 1111 Asp Asn Thr AlaGln Asp Gln Leu Leu Leu Gln His Leu Ser Thr Phe 100 105 110 GAG TTA AACGCG CAA TTA TTG ACT AAA TTA CCA AGC GAA GTG AGT GGC 1159 Glu Leu Asn AlaGln Leu Leu Thr Lys Leu Pro Ser Glu Val Ser Gly 115 120 125 130 GGA CAATTA CAA AGA TTT AAT GTC ATG CGT TCG TTA TTA GCA CAG CCT 1207 Gly Gln LeuGln Arg Phe Asn Val Met Arg Ser Leu Leu Ala Gln Pro 135 140 145 CGC GTTTTA ATA TGT GAT GAG ATA ACT TCA AAT TTA GAT GTT ATA GCT 1255 Arg Val LeuIle Cys Asp Glu Ile Thr Ser Asn Leu Asp Val Ile Ala 150 155 160 GAA CAAAAT GTA ATC AAT ATA TTA AAA GCG CAA ACG ATT ACG AAC TTA 1303 Glu Gln AsnVal Ile Asn Ile Leu Lys Ala Gln Thr Ile Thr Asn Leu 165 170 175 AAT CATTTT ATC GTT ATT TCT CAT GAT TTA TCC GTG TTA CAA CGC TTA 1351 Asn His PheIle Val Ile Ser His Asp Leu Ser Val Leu Gln Arg Leu 180 185 190 GTT AATAGA ATT ATC GTT CTT AAG GAT GGC ATG ATA GTC GAT GAT TTT 1399 Val Asn ArgIle Ile Val Leu Lys Asp Gly Met Ile Val Asp Asp Phe 195 200 205 210 GCAATA GAG GAA TTA TTT AAT GTT GAT AGA CAC CCT TAT ACA AAA GAA 1447 Ala IleGlu Glu Leu Phe Asn Val Asp Arg His Pro Tyr Thr Lys Glu 215 220 225 TTAGTG CAA GCA TTT TCA TAT TAG 1471 Leu Val Gln Ala Phe Ser Tyr 230 233amino acids amino acid linear protein 20 Met Ile Glu Leu Lys His Val ThrPhe Gly Tyr Asn Lys Lys Gln Met 1 5 10 15 Val Leu Gln Asp Ile Asn IleThr Ile Pro Asp Gly Glu Asn Val Gly 20 25 30 Ile Leu Gly Glu Ser Gly CysGly Lys Ser Thr Leu Ala Ser Leu Val 35 40 45 Leu Gly Leu Phe Lys Pro ValLys Gly Glu Ile Tyr Leu Ser Asp Asn 50 55 60 Ala Val Leu Pro Ile Phe GlnHis Pro Leu Thr Ser Phe Asn Pro Asp 65 70 75 80 Trp Thr Ile Glu Thr SerLeu Lys Glu Ala Leu Tyr Tyr Tyr Arg Gly 85 90 95 Leu Thr Asp Asn Thr AlaGln Asp Gln Leu Leu Leu Gln His Leu Ser 100 105 110 Thr Phe Glu Leu AsnAla Gln Leu Leu Thr Lys Leu Pro Ser Glu Val 115 120 125 Ser Gly Gly GlnLeu Gln Arg Phe Asn Val Met Arg Ser Leu Leu Ala 130 135 140 Gln Pro ArgVal Leu Ile Cys Asp Glu Ile Thr Ser Asn Leu Asp Val 145 150 155 160 IleAla Glu Gln Asn Val Ile Asn Ile Leu Lys Ala Gln Thr Ile Thr 165 170 175Asn Leu Asn His Phe Ile Val Ile Ser His Asp Leu Ser Val Leu Gln 180 185190 Arg Leu Val Asn Arg Ile Ile Val Leu Lys Asp Gly Met Ile Val Asp 195200 205 Asp Phe Ala Ile Glu Glu Leu Phe Asn Val Asp Arg His Pro Tyr Thr210 215 220 Lys Glu Leu Val Gln Ala Phe Ser Tyr 225 230 396 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p13b74)“ 21 AATCATAAAT TACGAATTAG ATATAACAAA AAAGAGCTTG GGACATTAAGTCCTTAAAGT 60 CTTAGGCAAT GTAAAAAAGC TGATTTCTAT TATTTATTTG ATAGAAATCAGCTTTTTTGA 120 TATGTATTTT ATAATGTACA GCTCGTTGAG CTGCTATTTT CCTTATATTAAGTGCCATTA 180 ATACAAAACC TAGCTCTCGT TTAACTTTAT TTATTCCCGA ACTGACATTCGAGTGAAACC 240 CAAAATAGCC TTCTAATCCA AAAACAGGCT CTACTCAATT TTCCTTTTGACTAATAGATT 300 TTTTCGTTTC TGGTTCCAAA GCCTTGAGGT TGTAGAATTC TTGAAGACGAAAAGGGCCCC 360 GTGATACGCC CTATTTTTAA AGGTTAATGT TCTGAA 396 1845 basepairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p14c15)“ CDS 3..788 CDS 856..1842 22 GC ATC AAT GAA CAT CAGGAA TTA ACA ACT TTA GGC AGA GGT GGT TCT 47 Ile Asn Glu His Gln Glu LeuThr Thr Leu Gly Arg Gly Gly Ser 1 5 10 15 GAT ACG ACC GCT GTG GCA CTTGCT GTT AGT AAT CAA ATA CCT TGT GAA 95 Asp Thr Thr Ala Val Ala Leu AlaVal Ser Asn Gln Ile Pro Cys Glu 20 25 30 ATT TAT ACC GAC GTT GAT GGT GTGTAT GCC ACT GAC CCA AGA CTT TTA 143 Ile Tyr Thr Asp Val Asp Gly Val TyrAla Thr Asp Pro Arg Leu Leu 35 40 45 CCA AAG GCT AAA CGA CTA GAC ATC GTCTCA TAT GAA GAA ATG ATG GAA 191 Pro Lys Ala Lys Arg Leu Asp Ile Val SerTyr Glu Glu Met Met Glu 50 55 60 ATG AGC GCT TTA GGT GCT GGT GTA CTT GAAACA AGA AGT GTT GAA TTA 239 Met Ser Ala Leu Gly Ala Gly Val Leu Glu ThrArg Ser Val Glu Leu 65 70 75 GCT AAA AAC TAT AAT ATC CCT TTA TAT TTA GGAAAA ACT TTA TCG AAC 287 Ala Lys Asn Tyr Asn Ile Pro Leu Tyr Leu Gly LysThr Leu Ser Asn 80 85 90 95 GTG AAA GGA ACA TGG ATT ATG TCA AAT GAA GAAATA TTA GAG AAA AAA 335 Val Lys Gly Thr Trp Ile Met Ser Asn Glu Glu IleLeu Glu Lys Lys 100 105 110 GCA GTT ACT GGT GTG GCT TTG GAT AAA CAT ATGATG CAT GTA ACA ATT 383 Ala Val Thr Gly Val Ala Leu Asp Lys His Met MetHis Val Thr Ile 115 120 125 AGT TAT CCC CTA CCT GAC AAT CAG CTA CTT ACCCAA CTA TTT ACG GAA 431 Ser Tyr Pro Leu Pro Asp Asn Gln Leu Leu Thr GlnLeu Phe Thr Glu 130 135 140 CTT GAA GAA GGT GCT GTA AAT GTT GAT ATG ATTTCA CAA ATC GTC AAC 479 Leu Glu Glu Gly Ala Val Asn Val Asp Met Ile SerGln Ile Val Asn 145 150 155 TTG GAT GGG CTA CAA CTA TCC TTC ACG ATT AAAGAT AGT GAT TTT CAT 527 Leu Asp Gly Leu Gln Leu Ser Phe Thr Ile Lys AspSer Asp Phe His 160 165 170 175 CAA ATT TCT ATG ATT CTT GAA ACA TTA AAGAAT CAA TAT GAA GCA TTA 575 Gln Ile Ser Met Ile Leu Glu Thr Leu Lys AsnGln Tyr Glu Ala Leu 180 185 190 GCT TAT AAA ATC AAT GAG CAT TAT GTC AAAATT TCA TTA ATT GGC TCA 623 Ala Tyr Lys Ile Asn Glu His Tyr Val Lys IleSer Leu Ile Gly Ser 195 200 205 GGC ATG CGT GAT ATG TCA GGT GTG GCA TCAAAA GCA TTT TTG ACA TTA 671 Gly Met Arg Asp Met Ser Gly Val Ala Ser LysAla Phe Leu Thr Leu 210 215 220 ATT GAA AAT AAT ATA CCT TTC TAC CAA ACAACA ACA TCT GAA ATA AGT 719 Ile Glu Asn Asn Ile Pro Phe Tyr Gln Thr ThrThr Ser Glu Ile Ser 225 230 235 240 ATT TCA TAC GTC ATT GAT GAT TTT AATGGG CAA CAA GCG GTA GAA AAA 767 Ile Ser Tyr Val Ile Asp Asp Phe Asn GlyGln Gln Ala Val Glu Lys 245 250 255 CTA TAT GAC GCA TTT AAC ATTTAATGGTAAA ATGATTGTTA AAATATTCTA 818 Leu Tyr Asp Ala Phe Asn Ile 260AAAATTGGGA AATTATTATA AAATGGAGTG ACAAGTT ATG ACA AAG TTA GCA GTT 873 MetThr Lys Leu Ala Val 1 5 GTG GGT GCA ACA GGA TTA GTA GGT ACA AAA ATG TTGGAG ACA TTA AAT 921 Val Gly Ala Thr Gly Leu Val Gly Thr Lys Met Leu GluThr Leu Asn 10 15 20 CGT AAA AAT ATT CCT TTC GAT GAA TTA GTA TTA TTT TCATCA GCA CGT 969 Arg Lys Asn Ile Pro Phe Asp Glu Leu Val Leu Phe Ser SerAla Arg 25 30 35 TCT GCA GGG CAA GAA GTT GAA TTT CAA GGA AAA ACA TAT ACAGTT CAA 1017 Ser Ala Gly Gln Glu Val Glu Phe Gln Gly Lys Thr Tyr Thr ValGln 40 45 50 GAA TTA ACT GAT GCT CGT GCA AGT GAA CAT TTC GAT TAT GTA TTAATG 1065 Glu Leu Thr Asp Ala Arg Ala Ser Glu His Phe Asp Tyr Val Leu Met55 60 65 70 AGT GCT GGT GGC GGT ACA AGC GAA CAC TTT GCC CCA CTT TTT GAAAAA 1113 Ser Ala Gly Gly Gly Thr Ser Glu His Phe Ala Pro Leu Phe Glu Lys75 80 85 GCT GGT GCA ATC GTT ATA GAC AAT TCA AGT CAA TGG CGT ATG GCA GAA1161 Ala Gly Ala Ile Val Ile Asp Asn Ser Ser Gln Trp Arg Met Ala Glu 9095 100 GAT ATT GAT TTA ATC GTT CCG GAA GTC AAT GAA CCT ACA TTT ACA AGA1209 Asp Ile Asp Leu Ile Val Pro Glu Val Asn Glu Pro Thr Phe Thr Arg 105110 115 GGT ATC ATT GCC AAT CCA AAC TGC TCT ACG ATT CAA TCT GTT GTA CCT1257 Gly Ile Ile Ala Asn Pro Asn Cys Ser Thr Ile Gln Ser Val Val Pro 120125 130 CTA AAA GTA TTG CAA GAT GCT TAT GGT TTA AAA CGA GTG GCA TAT ACA1305 Leu Lys Val Leu Gln Asp Ala Tyr Gly Leu Lys Arg Val Ala Tyr Thr 135140 145 150 ACA TAT CAA GCT GTA TCA GGT TCA GGG ATG AAA GGT AAG AAA GATTTA 1353 Thr Tyr Gln Ala Val Ser Gly Ser Gly Met Lys Gly Lys Lys Asp Leu155 160 165 GCT GAA GGT GTA AAT GGT AAA GCA CCA GAA GCA TAT CCA CAT CCAATT 1401 Ala Glu Gly Val Asn Gly Lys Ala Pro Glu Ala Tyr Pro His Pro Ile170 175 180 TAT AAT AAT GTG TTA CCG CAT ATT GAT GTG TTT TTA GAA AAC GGATAT 1449 Tyr Asn Asn Val Leu Pro His Ile Asp Val Phe Leu Glu Asn Gly Tyr185 190 195 ACA AAA GAA GAA CAA AAA ATG ATT GAT GAG ACG AGA AAA ATT TTAAAT 1497 Thr Lys Glu Glu Gln Lys Met Ile Asp Glu Thr Arg Lys Ile Leu Asn200 205 210 GCG CCA GAC TTA AAA GTA ACA GCA ACA TGC GCA CGT GTG CCT GTTCAA 1545 Ala Pro Asp Leu Lys Val Thr Ala Thr Cys Ala Arg Val Pro Val Gln215 220 225 230 GAT AGT CAT AGT GTT GAA ATT GAT GTA ACG CTT GAC AAA GAAACA ACA 1593 Asp Ser His Ser Val Glu Ile Asp Val Thr Leu Asp Lys Glu ThrThr 235 240 245 GCA GAA GAT ATT AAA GCG TTA TTT GAT CAA GAT GAC CGC GTTGTT TTA 1641 Ala Glu Asp Ile Lys Ala Leu Phe Asp Gln Asp Asp Arg Val ValLeu 250 255 260 GTA GAC AAT CCA GAG AAC AAT GAA TAT CCA ATG GCA ATC AATTCT ACT 1689 Val Asp Asn Pro Glu Asn Asn Glu Tyr Pro Met Ala Ile Asn SerThr 265 270 275 AAT AAA GAT GAA GTG TTT GTT GGC CGT ATA CGT AGA GAT GATTCA TTA 1737 Asn Lys Asp Glu Val Phe Val Gly Arg Ile Arg Arg Asp Asp SerLeu 280 285 290 GAA AAT ACT TTC CAT GTA TGG TGT ACA TCA GAC AAT TTA TTAAAA GGT 1785 Glu Asn Thr Phe His Val Trp Cys Thr Ser Asp Asn Leu Leu LysGly 295 300 305 310 GCT GCA TTA AAT GCT GTA CAA GTA TTG GAA CAA GTT ATGCGT TTA AAA 1833 Ala Ala Leu Asn Ala Val Gln Val Leu Glu Gln Val Met ArgLeu Lys 315 320 325 GGA GCG AAT TAA 1845 Gly Ala Asn 262 amino acidsamino acid linear protein 23 Ile Asn Glu His Gln Glu Leu Thr Thr Leu GlyArg Gly Gly Ser Asp 1 5 10 15 Thr Thr Ala Val Ala Leu Ala Val Ser AsnGln Ile Pro Cys Glu Ile 20 25 30 Tyr Thr Asp Val Asp Gly Val Tyr Ala ThrAsp Pro Arg Leu Leu Pro 35 40 45 Lys Ala Lys Arg Leu Asp Ile Val Ser TyrGlu Glu Met Met Glu Met 50 55 60 Ser Ala Leu Gly Ala Gly Val Leu Glu ThrArg Ser Val Glu Leu Ala 65 70 75 80 Lys Asn Tyr Asn Ile Pro Leu Tyr LeuGly Lys Thr Leu Ser Asn Val 85 90 95 Lys Gly Thr Trp Ile Met Ser Asn GluGlu Ile Leu Glu Lys Lys Ala 100 105 110 Val Thr Gly Val Ala Leu Asp LysHis Met Met His Val Thr Ile Ser 115 120 125 Tyr Pro Leu Pro Asp Asn GlnLeu Leu Thr Gln Leu Phe Thr Glu Leu 130 135 140 Glu Glu Gly Ala Val AsnVal Asp Met Ile Ser Gln Ile Val Asn Leu 145 150 155 160 Asp Gly Leu GlnLeu Ser Phe Thr Ile Lys Asp Ser Asp Phe His Gln 165 170 175 Ile Ser MetIle Leu Glu Thr Leu Lys Asn Gln Tyr Glu Ala Leu Ala 180 185 190 Tyr LysIle Asn Glu His Tyr Val Lys Ile Ser Leu Ile Gly Ser Gly 195 200 205 MetArg Asp Met Ser Gly Val Ala Ser Lys Ala Phe Leu Thr Leu Ile 210 215 220Glu Asn Asn Ile Pro Phe Tyr Gln Thr Thr Thr Ser Glu Ile Ser Ile 225 230235 240 Ser Tyr Val Ile Asp Asp Phe Asn Gly Gln Gln Ala Val Glu Lys Leu245 250 255 Tyr Asp Ala Phe Asn Ile 260 329 amino acids amino acidlinear protein 24 Met Thr Lys Leu Ala Val Val Gly Ala Thr Gly Leu ValGly Thr Lys 1 5 10 15 Met Leu Glu Thr Leu Asn Arg Lys Asn Ile Pro PheAsp Glu Leu Val 20 25 30 Leu Phe Ser Ser Ala Arg Ser Ala Gly Gln Glu ValGlu Phe Gln Gly 35 40 45 Lys Thr Tyr Thr Val Gln Glu Leu Thr Asp Ala ArgAla Ser Glu His 50 55 60 Phe Asp Tyr Val Leu Met Ser Ala Gly Gly Gly ThrSer Glu His Phe 65 70 75 80 Ala Pro Leu Phe Glu Lys Ala Gly Ala Ile ValIle Asp Asn Ser Ser 85 90 95 Gln Trp Arg Met Ala Glu Asp Ile Asp Leu IleVal Pro Glu Val Asn 100 105 110 Glu Pro Thr Phe Thr Arg Gly Ile Ile AlaAsn Pro Asn Cys Ser Thr 115 120 125 Ile Gln Ser Val Val Pro Leu Lys ValLeu Gln Asp Ala Tyr Gly Leu 130 135 140 Lys Arg Val Ala Tyr Thr Thr TyrGln Ala Val Ser Gly Ser Gly Met 145 150 155 160 Lys Gly Lys Lys Asp LeuAla Glu Gly Val Asn Gly Lys Ala Pro Glu 165 170 175 Ala Tyr Pro His ProIle Tyr Asn Asn Val Leu Pro His Ile Asp Val 180 185 190 Phe Leu Glu AsnGly Tyr Thr Lys Glu Glu Gln Lys Met Ile Asp Glu 195 200 205 Thr Arg LysIle Leu Asn Ala Pro Asp Leu Lys Val Thr Ala Thr Cys 210 215 220 Ala ArgVal Pro Val Gln Asp Ser His Ser Val Glu Ile Asp Val Thr 225 230 235 240Leu Asp Lys Glu Thr Thr Ala Glu Asp Ile Lys Ala Leu Phe Asp Gln 245 250255 Asp Asp Arg Val Val Leu Val Asp Asn Pro Glu Asn Asn Glu Tyr Pro 260265 270 Met Ala Ile Asn Ser Thr Asn Lys Asp Glu Val Phe Val Gly Arg Ile275 280 285 Arg Arg Asp Asp Ser Leu Glu Asn Thr Phe His Val Trp Cys ThrSer 290 295 300 Asp Asn Leu Leu Lys Gly Ala Ala Leu Asn Ala Val Gln ValLeu Glu 305 310 315 320 Gln Val Met Arg Leu Lys Gly Ala Asn 325 927 basepairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p13b26)“ CDS 1..924 25 ATG AAC GAA GCC GAC ATG CTG TTC TCTGTC ACT GTT CCC GGA AGC ACA 48 Met Asn Glu Ala Asp Met Leu Phe Ser ValThr Val Pro Gly Ser Thr 1 5 10 15 GCT AAC CTA GGC CCC GGC TTT GAT TCAGTC GGA ATG GCG CTC AGC AGA 96 Ala Asn Leu Gly Pro Gly Phe Asp Ser ValGly Met Ala Leu Ser Arg 20 25 30 TAT TTG AAG CTG ACC GTC TTT GAA AGC GACAAA TGG TCT TTT GAG GCT 144 Tyr Leu Lys Leu Thr Val Phe Glu Ser Asp LysTrp Ser Phe Glu Ala 35 40 45 GAA ACA GAA ACA GTC GCC GGA ATT CGC GGT ACAGAT AAC CTG ATC TAC 192 Glu Thr Glu Thr Val Ala Gly Ile Arg Gly Thr AspAsn Leu Ile Tyr 50 55 60 CAA GTG GCT AAA CGG ACC GCA GAT TTG TAC GGA AAAGAA ATG CCT CCT 240 Gln Val Ala Lys Arg Thr Ala Asp Leu Tyr Gly Lys GluMet Pro Pro 65 70 75 80 GTC CAT GTG AAG GTG TGG AGC GAC ATC CCG CTT GCACGC GGC CTT GGC 288 Val His Val Lys Val Trp Ser Asp Ile Pro Leu Ala ArgGly Leu Gly 85 90 95 AGC AGC GCC GCA GCG ATT GTA GCG GCC ATT GAA CTG GCTGAT GAA TTA 336 Ser Ser Ala Ala Ala Ile Val Ala Ala Ile Glu Leu Ala AspGlu Leu 100 105 110 TGC GGC TTA AAG CTG TCT GAA GCG GAC AAG CTG CAT TTAGCG AGT CTA 384 Cys Gly Leu Lys Leu Ser Glu Ala Asp Lys Leu His Leu AlaSer Leu 115 120 125 GAA GAA GGA CAC CCG GAC AAT GCT GGC GCT TCT CTC GTCGGC GGA CTT 432 Glu Glu Gly His Pro Asp Asn Ala Gly Ala Ser Leu Val GlyGly Leu 130 135 140 GTG ATC GGC CTG CAT GAG GAT GAC GAG ACC CAA ATG ATCCGC GTC CCG 480 Val Ile Gly Leu His Glu Asp Asp Glu Thr Gln Met Ile ArgVal Pro 145 150 155 160 AAT GCT GAC ATT GAC GTA GTC GTT GTC ATT CCT TTTTAT GAA GTG CTG 528 Asn Ala Asp Ile Asp Val Val Val Val Ile Pro Phe TyrGlu Val Leu 165 170 175 ACA AGA GAC GCG AGA GAC GTG CTT CCG AAG GAG TTTCCA TAT GCC GAT 576 Thr Arg Asp Ala Arg Asp Val Leu Pro Lys Glu Phe ProTyr Ala Asp 180 185 190 GCC GTA AAA GCA AGT GCT GTC AGC AAT ATC CTC ATTGCT GCG ATC ATG 624 Ala Val Lys Ala Ser Ala Val Ser Asn Ile Leu Ile AlaAla Ile Met 195 200 205 TCC AAG GAT TGG CCG CTT GTC GGG AAA ATC ATG AAGAAG GAT ATG TTC 672 Ser Lys Asp Trp Pro Leu Val Gly Lys Ile Met Lys LysAsp Met Phe 210 215 220 CAT CAG CCG TAC CGG GCG ATG CTT GTG CCT GAG CTGTCA AAA GTA GAG 720 His Gln Pro Tyr Arg Ala Met Leu Val Pro Glu Leu SerLys Val Glu 225 230 235 240 CAC GTC GCC GAG ATG AAG GGC GCA TAT GGA ACGGCT CTC AGC GGA GCA 768 His Val Ala Glu Met Lys Gly Ala Tyr Gly Thr AlaLeu Ser Gly Ala 245 250 255 GGC CCA ACG ATT CTC GTC ATG ACC GAA AAA GGAAAG GGA GAA GAG CTA 816 Gly Pro Thr Ile Leu Val Met Thr Glu Lys Gly LysGly Glu Glu Leu 260 265 270 AAA GAA CAG CTC GCG CTT CAT TTC CCT CAT TGTGAA GTA GAC GCT TTG 864 Lys Glu Gln Leu Ala Leu His Phe Pro His Cys GluVal Asp Ala Leu 275 280 285 ACC GTT CCG AAA GAG GGA AGT ATA ATA GAG CGAAAT CCT TTA TAT CAA 912 Thr Val Pro Lys Glu Gly Ser Ile Ile Glu Arg AsnPro Leu Tyr Gln 290 295 300 GTA AAA AGT GTA TAG 927 Val Lys Ser Val 305308 amino acids amino acid linear protein 26 Met Asn Glu Ala Asp Met LeuPhe Ser Val Thr Val Pro Gly Ser Thr 1 5 10 15 Ala Asn Leu Gly Pro GlyPhe Asp Ser Val Gly Met Ala Leu Ser Arg 20 25 30 Tyr Leu Lys Leu Thr ValPhe Glu Ser Asp Lys Trp Ser Phe Glu Ala 35 40 45 Glu Thr Glu Thr Val AlaGly Ile Arg Gly Thr Asp Asn Leu Ile Tyr 50 55 60 Gln Val Ala Lys Arg ThrAla Asp Leu Tyr Gly Lys Glu Met Pro Pro 65 70 75 80 Val His Val Lys ValTrp Ser Asp Ile Pro Leu Ala Arg Gly Leu Gly 85 90 95 Ser Ser Ala Ala AlaIle Val Ala Ala Ile Glu Leu Ala Asp Glu Leu 100 105 110 Cys Gly Leu LysLeu Ser Glu Ala Asp Lys Leu His Leu Ala Ser Leu 115 120 125 Glu Glu GlyHis Pro Asp Asn Ala Gly Ala Ser Leu Val Gly Gly Leu 130 135 140 Val IleGly Leu His Glu Asp Asp Glu Thr Gln Met Ile Arg Val Pro 145 150 155 160Asn Ala Asp Ile Asp Val Val Val Val Ile Pro Phe Tyr Glu Val Leu 165 170175 Thr Arg Asp Ala Arg Asp Val Leu Pro Lys Glu Phe Pro Tyr Ala Asp 180185 190 Ala Val Lys Ala Ser Ala Val Ser Asn Ile Leu Ile Ala Ala Ile Met195 200 205 Ser Lys Asp Trp Pro Leu Val Gly Lys Ile Met Lys Lys Asp MetPhe 210 215 220 His Gln Pro Tyr Arg Ala Met Leu Val Pro Glu Leu Ser LysVal Glu 225 230 235 240 His Val Ala Glu Met Lys Gly Ala Tyr Gly Thr AlaLeu Ser Gly Ala 245 250 255 Gly Pro Thr Ile Leu Val Met Thr Glu Lys GlyLys Gly Glu Glu Leu 260 265 270 Lys Glu Gln Leu Ala Leu His Phe Pro HisCys Glu Val Asp Ala Leu 275 280 285 Thr Val Pro Lys Glu Gly Ser Ile IleGlu Arg Asn Pro Leu Tyr Gln 290 295 300 Val Lys Ser Val 305 1320 basepairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p7c18)“ CDS 1..1317 27 TTG TTC TTA CAC GGC ACA AGC AGA CAAAAT CAA CAT GGT CAT TTA GAA 48 Leu Phe Leu His Gly Thr Ser Arg Gln AsnGln His Gly His Leu Glu 1 5 10 15 ATC GGA GGT GTG GAT GCT CTC TAT TTAGCG GAG AAA TAT GGT ACA CCT 96 Ile Gly Gly Val Asp Ala Leu Tyr Leu AlaGlu Lys Tyr Gly Thr Pro 20 25 30 CTT TAC GTA TAT GAT GTG GCT TTA ATA CGTGAG CGT GCT AAA AGC TTT 144 Leu Tyr Val Tyr Asp Val Ala Leu Ile Arg GluArg Ala Lys Ser Phe 35 40 45 AAG CAG GCG TTT ATT TCT GCA GGG CTG AAA GCACAG GTG GCA TAT GCG 192 Lys Gln Ala Phe Ile Ser Ala Gly Leu Lys Ala GlnVal Ala Tyr Ala 50 55 60 AGC AAA GCA TTC TCA TCA GTC GCA ATG ATT CAG CTCGCT GAG GAA GAG 240 Ser Lys Ala Phe Ser Ser Val Ala Met Ile Gln Leu AlaGlu Glu Glu 65 70 75 80 GGA CTT TCT TTA GAT GTC GTA TCC GGA GGA GAG CTATAT ACG GCT GTT 288 Gly Leu Ser Leu Asp Val Val Ser Gly Gly Glu Leu TyrThr Ala Val 85 90 95 GCA GCA GGC TTT CCG GCA GAA CGC ATC CAC TTT CAT GGAAAC AAT AAG 336 Ala Ala Gly Phe Pro Ala Glu Arg Ile His Phe His Gly AsnAsn Lys 100 105 110 AGC AGG GAA GAA CTG CGG ATG GCG CTT GAG CAC CGC ATCGGC TGC ATT 384 Ser Arg Glu Glu Leu Arg Met Ala Leu Glu His Arg Ile GlyCys Ile 115 120 125 GTG GTG GAT AAT TTC TAT GAA ATC GCG CTT CTT GAA GACCTA TGT AAA 432 Val Val Asp Asn Phe Tyr Glu Ile Ala Leu Leu Glu Asp LeuCys Lys 130 135 140 GAA ACG GGT CAC TCC ATC GAT GTT CTT CTT CGG ATC ACGCCC GGA GTA 480 Glu Thr Gly His Ser Ile Asp Val Leu Leu Arg Ile Thr ProGly Val 145 150 155 160 GAA GCG CAT ACG CAT GAC TAC ATT ACA ACG GGC CAGGAA GAT TCA AAG 528 Glu Ala His Thr His Asp Tyr Ile Thr Thr Gly Gln GluAsp Ser Lys 165 170 175 TTT GGT TTC GAT CTT CAT AAC GGA CAA ACT GAA CGGGCC ATT GAA CAA 576 Phe Gly Phe Asp Leu His Asn Gly Gln Thr Glu Arg AlaIle Glu Gln 180 185 190 GTA TTA CAA TCG GAA CAC ATT CAG CTG CTG GGT GTCCAT TGC CAT ATC 624 Val Leu Gln Ser Glu His Ile Gln Leu Leu Gly Val HisCys His Ile 195 200 205 GGC TCG CAA ATC TTT GAT ACG GCC GGT TTT GTG TTAGCA GCG GAA AAA 672 Gly Ser Gln Ile Phe Asp Thr Ala Gly Phe Val Leu AlaAla Glu Lys 210 215 220 ATC TTC AAA AAA CTA GAC GAA TGG AGA GAT TCA TATTCA TTT GTA TCC 720 Ile Phe Lys Lys Leu Asp Glu Trp Arg Asp Ser Tyr SerPhe Val Ser 225 230 235 240 AAG GTG CTG AAT CTT GGA GGA GGT TTC GGC ATTCGT TAT ACG GAA GAT 768 Lys Val Leu Asn Leu Gly Gly Gly Phe Gly Ile ArgTyr Thr Glu Asp 245 250 255 GAT GAA CCG CTT CAT GCC ACT GAA TAC GTT GAAAAA ATT ATC GAA GCT 816 Asp Glu Pro Leu His Ala Thr Glu Tyr Val Glu LysIle Ile Glu Ala 260 265 270 GTG AAA GAA AAT GCT TCC CGT TAC GGT TTT GACATT CCG GAA ATT TGG 864 Val Lys Glu Asn Ala Ser Arg Tyr Gly Phe Asp IlePro Glu Ile Trp 275 280 285 ATC GAA CCG GGC CGT TCT CTC GTG GGA GAC GCAGGC ACA ACT CTT TAT 912 Ile Glu Pro Gly Arg Ser Leu Val Gly Asp Ala GlyThr Thr Leu Tyr 290 295 300 ACG GTT GGC TCT CAA AAA GAA GTG CCG GGT GTCCGC CAA TAT GTG GCT 960 Thr Val Gly Ser Gln Lys Glu Val Pro Gly Val ArgGln Tyr Val Ala 305 310 315 320 GTA GAC GGA GGC ATG AAC GAC AAT ATT CGTCCT GCG CTT TAC CAA GCT 1008 Val Asp Gly Gly Met Asn Asp Asn Ile Arg ProAla Leu Tyr Gln Ala 325 330 335 AAA TAT GAA GCT GCG GCA GCC AAC AGG ATCGGA GAA GCG CAT GAC AAA 1056 Lys Tyr Glu Ala Ala Ala Ala Asn Arg Ile GlyGlu Ala His Asp Lys 340 345 350 ACG GTA TCA ATT GCC GGA AAG TGC TGT GAAAGC GGA GAT ATG CTG ATT 1104 Thr Val Ser Ile Ala Gly Lys Cys Cys Glu SerGly Asp Met Leu Ile 355 360 365 TGG GAT ATT GAC CTG CCG GAA GTA AAA GAAGGC GAT CTT CTT GCC GTT 1152 Trp Asp Ile Asp Leu Pro Glu Val Lys Glu GlyAsp Leu Leu Ala Val 370 375 380 TTT TGT ACA GGC GCT TAT GGA TAC AGC ATGGCC AAC AAT TAT AAC CGT 1200 Phe Cys Thr Gly Ala Tyr Gly Tyr Ser Met AlaAsn Asn Tyr Asn Arg 385 390 395 400 ATT CCG AGA CCC GCC GTT GTA TTT GTCGAA AAC GGT GAG GCT CAT TTA 1248 Ile Pro Arg Pro Ala Val Val Phe Val GluAsn Gly Glu Ala His Leu 405 410 415 GTC GTG AAG CGA GAA ACA TAC GAA GATATT GTA AAA CTT GAT CTG CCA 1296 Val Val Lys Arg Glu Thr Tyr Glu Asp IleVal Lys Leu Asp Leu Pro 420 425 430 TTT AAA ACG GGT GTA AAG CAA TAA 1320Phe Lys Thr Gly Val Lys Gln 435 439 amino acids amino acid linearprotein 28 Leu Phe Leu His Gly Thr Ser Arg Gln Asn Gln His Gly His LeuGlu 1 5 10 15 Ile Gly Gly Val Asp Ala Leu Tyr Leu Ala Glu Lys Tyr GlyThr Pro 20 25 30 Leu Tyr Val Tyr Asp Val Ala Leu Ile Arg Glu Arg Ala LysSer Phe 35 40 45 Lys Gln Ala Phe Ile Ser Ala Gly Leu Lys Ala Gln Val AlaTyr Ala 50 55 60 Ser Lys Ala Phe Ser Ser Val Ala Met Ile Gln Leu Ala GluGlu Glu 65 70 75 80 Gly Leu Ser Leu Asp Val Val Ser Gly Gly Glu Leu TyrThr Ala Val 85 90 95 Ala Ala Gly Phe Pro Ala Glu Arg Ile His Phe His GlyAsn Asn Lys 100 105 110 Ser Arg Glu Glu Leu Arg Met Ala Leu Glu His ArgIle Gly Cys Ile 115 120 125 Val Val Asp Asn Phe Tyr Glu Ile Ala Leu LeuGlu Asp Leu Cys Lys 130 135 140 Glu Thr Gly His Ser Ile Asp Val Leu LeuArg Ile Thr Pro Gly Val 145 150 155 160 Glu Ala His Thr His Asp Tyr IleThr Thr Gly Gln Glu Asp Ser Lys 165 170 175 Phe Gly Phe Asp Leu His AsnGly Gln Thr Glu Arg Ala Ile Glu Gln 180 185 190 Val Leu Gln Ser Glu HisIle Gln Leu Leu Gly Val His Cys His Ile 195 200 205 Gly Ser Gln Ile PheAsp Thr Ala Gly Phe Val Leu Ala Ala Glu Lys 210 215 220 Ile Phe Lys LysLeu Asp Glu Trp Arg Asp Ser Tyr Ser Phe Val Ser 225 230 235 240 Lys ValLeu Asn Leu Gly Gly Gly Phe Gly Ile Arg Tyr Thr Glu Asp 245 250 255 AspGlu Pro Leu His Ala Thr Glu Tyr Val Glu Lys Ile Ile Glu Ala 260 265 270Val Lys Glu Asn Ala Ser Arg Tyr Gly Phe Asp Ile Pro Glu Ile Trp 275 280285 Ile Glu Pro Gly Arg Ser Leu Val Gly Asp Ala Gly Thr Thr Leu Tyr 290295 300 Thr Val Gly Ser Gln Lys Glu Val Pro Gly Val Arg Gln Tyr Val Ala305 310 315 320 Val Asp Gly Gly Met Asn Asp Asn Ile Arg Pro Ala Leu TyrGln Ala 325 330 335 Lys Tyr Glu Ala Ala Ala Ala Asn Arg Ile Gly Glu AlaHis Asp Lys 340 345 350 Thr Val Ser Ile Ala Gly Lys Cys Cys Glu Ser GlyAsp Met Leu Ile 355 360 365 Trp Asp Ile Asp Leu Pro Glu Val Lys Glu GlyAsp Leu Leu Ala Val 370 375 380 Phe Cys Thr Gly Ala Tyr Gly Tyr Ser MetAla Asn Asn Tyr Asn Arg 385 390 395 400 Ile Pro Arg Pro Ala Val Val PheVal Glu Asn Gly Glu Ala His Leu 405 410 415 Val Val Lys Arg Glu Thr TyrGlu Asp Ile Val Lys Leu Asp Leu Pro 420 425 430 Phe Lys Thr Gly Val LysGln 435 2190 base pairs nucleic acid single linear other nucleic acid/desc = ”DNA (genomic) (p15c31)“ CDS 1..2187 29 ATG TCT AAA TTT ATC GAACCA AGC GTT GAA GAA ATT AAA CTT GAA AAA 48 Met Ser Lys Phe Ile Glu ProSer Val Glu Glu Ile Lys Leu Glu Lys 1 5 10 15 GTA TAT CAA GAT ATG GGATTA AGT GAT CAA GAA TAT GAA AAA GTT TGC 96 Val Tyr Gln Asp Met Gly LeuSer Asp Gln Glu Tyr Glu Lys Val Cys 20 25 30 GAT ATT TTA GGC AGA CAA CCTAAC TTT ACA GAA ACA GGT ATC TTT TCT 144 Asp Ile Leu Gly Arg Gln Pro AsnPhe Thr Glu Thr Gly Ile Phe Ser 35 40 45 GTT ATG TGG AGT GAA CAT TGC TCTTAT AAA CAT TCT AAA CCG TTT TTA 192 Val Met Trp Ser Glu His Cys Ser TyrLys His Ser Lys Pro Phe Leu 50 55 60 AAG CAA TTT CCT ACG TCA GGT GAC CATGTG CTT ATG GGG CCT GGT GAA 240 Lys Gln Phe Pro Thr Ser Gly Asp His ValLeu Met Gly Pro Gly Glu 65 70 75 80 GGT GCA GGG GTA GTC GAT ATA GGT GATAAT CAA GCC GTA GTA TTT AAA 288 Gly Ala Gly Val Val Asp Ile Gly Asp AsnGln Ala Val Val Phe Lys 85 90 95 GTA GAG TCT CAC AAT CAT CCA TCA GCA ATTGAA CCA TAT CAA GGG GCT 336 Val Glu Ser His Asn His Pro Ser Ala Ile GluPro Tyr Gln Gly Ala 100 105 110 GCT ACA GGC GTT GGT GGA ATC ATT CGT GACATT GTC TCT ATT GGG GCT 384 Ala Thr Gly Val Gly Gly Ile Ile Arg Asp IleVal Ser Ile Gly Ala 115 120 125 AGA CCT ATT AAT TTG TTA AAC AGT CTT AGATTT GGA GAA TTA GAT AAT 432 Arg Pro Ile Asn Leu Leu Asn Ser Leu Arg PheGly Glu Leu Asp Asn 130 135 140 AAA CAA AAC CAA AGA TTA CTT AAA GGT GTTGTA AAG GGT ATC GGA GGT 480 Lys Gln Asn Gln Arg Leu Leu Lys Gly Val ValLys Gly Ile Gly Gly 145 150 155 160 TAT GGT AAC TGC ATT GGT ATT CCA ACAACT GCT GGT GAA ATC GAA TTT 528 Tyr Gly Asn Cys Ile Gly Ile Pro Thr ThrAla Gly Glu Ile Glu Phe 165 170 175 GAT GAA CGT TAT GAT GGC AAT CCA CTTGTT AAT GCA ATG TGT GTT GGT 576 Asp Glu Arg Tyr Asp Gly Asn Pro Leu ValAsn Ala Met Cys Val Gly 180 185 190 GTT ATC AAT CAC GAC ATG ATT CAA AAAGGC ACA GCA AAA GGT GTA GGT 624 Val Ile Asn His Asp Met Ile Gln Lys GlyThr Ala Lys Gly Val Gly 195 200 205 AAT TCG GTC ATT TAT GTT GGT TTG AAAACT GGT CGA GAT GGT ATT CAT 672 Asn Ser Val Ile Tyr Val Gly Leu Lys ThrGly Arg Asp Gly Ile His 210 215 220 GGT GCT ACT TTT GCA TCT GAA GAA TTGACG GAA GAA AGC GAA AGT AAA 720 Gly Ala Thr Phe Ala Ser Glu Glu Leu ThrGlu Glu Ser Glu Ser Lys 225 230 235 240 CGA CCT TCT GTA CAA ATC GGT GATCCA TTT GTA GGT AAA AAA TTA ATG 768 Arg Pro Ser Val Gln Ile Gly Asp ProPhe Val Gly Lys Lys Leu Met 245 250 255 GAA GCA ACA CTT GAA GCA ATT ACATTT GAT GAA TTA GTT GGT ATT CAA 816 Glu Ala Thr Leu Glu Ala Ile Thr PheAsp Glu Leu Val Gly Ile Gln 260 265 270 GAT ATG GGT GCT GCT GGT TTA ACATCT TCA TCG TCT GAA ATG GCG GCA 864 Asp Met Gly Ala Ala Gly Leu Thr SerSer Ser Ser Glu Met Ala Ala 275 280 285 AAA GGT GGT AGT GGG TTA CAT TTGAGA TTA GAA CAA GTG CCA ACA CGT 912 Lys Gly Gly Ser Gly Leu His Leu ArgLeu Glu Gln Val Pro Thr Arg 290 295 300 GAG CCA GGT ATT TCT CCT TAT GAAATG ATG CTT TCA GAA ACT CAA GAA 960 Glu Pro Gly Ile Ser Pro Tyr Glu MetMet Leu Ser Glu Thr Gln Glu 305 310 315 320 CGT ATG TTA CTA GTT GTT GAAAAA GGT ACT GAA CAA AAA TTC TTA GAT 1008 Arg Met Leu Leu Val Val Glu LysGly Thr Glu Gln Lys Phe Leu Asp 325 330 335 TTA TTT GAT AAG CAC GAA TTGGAT AGT GCT GTT ATA GGT GAA GTT ACA 1056 Leu Phe Asp Lys His Glu Leu AspSer Ala Val Ile Gly Glu Val Thr 340 345 350 GAT ACA AAT CGT TTT GTT TTAACA TAT GAT GAC GAA GTT TAT GCT GAC 1104 Asp Thr Asn Arg Phe Val Leu ThrTyr Asp Asp Glu Val Tyr Ala Asp 355 360 365 ATT CCA GTT GAA CCA CTA GCTGAT GAA GCA CCT GTA TAT ATT TTA GAA 1152 Ile Pro Val Glu Pro Leu Ala AspGlu Ala Pro Val Tyr Ile Leu Glu 370 375 380 GGA GAA GAA AAA GAT TAT AATACT TCT AAA AAT GAT TAT ACA CAC ATC 1200 Gly Glu Glu Lys Asp Tyr Asn ThrSer Lys Asn Asp Tyr Thr His Ile 385 390 395 400 GAT GTT AAA GAT ACT TTCTTT AAA TTA CTT AAG CAT CCG ACT ATA GCA 1248 Asp Val Lys Asp Thr Phe PheLys Leu Leu Lys His Pro Thr Ile Ala 405 410 415 TCT AAA CAC TAT TTA TATGAT CAA TAC GAC CAA CAA GTT GGT GCC AAT 1296 Ser Lys His Tyr Leu Tyr AspGln Tyr Asp Gln Gln Val Gly Ala Asn 420 425 430 ACG ATA ATT AAG CCA GGACTT CAA GCA TCG GTA GTA CGT GTG GAA GGC 1344 Thr Ile Ile Lys Pro Gly LeuGln Ala Ser Val Val Arg Val Glu Gly 435 440 445 ACA AAT AAG GCA ATT GCTTCA ACA ATT GAT GGT GAA GCG CGT TAT GTA 1392 Thr Asn Lys Ala Ile Ala SerThr Ile Asp Gly Glu Ala Arg Tyr Val 450 455 460 TAT AAC AAT CCA TAT GAAGGT GGA AAG ATG GTA GTA GCT GAA GCT TAT 1440 Tyr Asn Asn Pro Tyr Glu GlyGly Lys Met Val Val Ala Glu Ala Tyr 465 470 475 480 CGA AAT TTA ATT GCCGTG GGT GCA ACA CCA TTA GCA ATG ACA GAT TGT 1488 Arg Asn Leu Ile Ala ValGly Ala Thr Pro Leu Ala Met Thr Asp Cys 485 490 495 TTA AAT TAT GGT TCTCCT GAA AAG AAA GAA ATC TAT CAA CAG TTG ATA 1536 Leu Asn Tyr Gly Ser ProGlu Lys Lys Glu Ile Tyr Gln Gln Leu Ile 500 505 510 GAT TCA ACG AAA GGTATG GCA GAA GCA TGC GAC ATT CTT AAG ACA CCA 1584 Asp Ser Thr Lys Gly MetAla Glu Ala Cys Asp Ile Leu Lys Thr Pro 515 520 525 GTA GTT TCT GGT AATGTA TCT TTA TAT AAC GAA ACG AAA GGT ACT TCT 1632 Val Val Ser Gly Asn ValSer Leu Tyr Asn Glu Thr Lys Gly Thr Ser 530 535 540 ATT TTC CCA ACA CCAGTT GTT GGA ATG GTA GGT TTG ATT GAA AAT GTA 1680 Ile Phe Pro Thr Pro ValVal Gly Met Val Gly Leu Ile Glu Asn Val 545 550 555 560 AAT TAT TTA AATGAT TTT GAA CCT CAA GTT GGA GAT AAA TTA TAT TTA 1728 Asn Tyr Leu Asn AspPhe Glu Pro Gln Val Gly Asp Lys Leu Tyr Leu 565 570 575 ATC GGT GAT ACTAAG GAC GAC TTT GGT GGT AGT CAA CTT GAA AAG TTA 1776 Ile Gly Asp Thr LysAsp Asp Phe Gly Gly Ser Gln Leu Glu Lys Leu 580 585 590 ATT TAT GGC AAAGTT AAT CAT GAA TTT GAG TCA TTA GAT TTG AGT TCA 1824 Ile Tyr Gly Lys ValAsn His Glu Phe Glu Ser Leu Asp Leu Ser Ser 595 600 605 GAA GTT GAA AAAGGT GAA TCA ATC AAG ACC GCT ATT CGT GAA GGA CTA 1872 Glu Val Glu Lys GlyGlu Ser Ile Lys Thr Ala Ile Arg Glu Gly Leu 610 615 620 TTA TCA CAT GTTCAA ACA GTT GGT AAA GGT GGC TTA CTG ATT ACC TTA 1920 Leu Ser His Val GlnThr Val Gly Lys Gly Gly Leu Leu Ile Thr Leu 625 630 635 640 GCT AAA CTAAGT GCG CAT TAC GGT TTA GGA TTA AAA TCT TCA ATA GAT 1968 Ala Lys Leu SerAla His Tyr Gly Leu Gly Leu Lys Ser Ser Ile Asp 645 650 655 ATA ACA AATGCA CAA TTG TTT AGT GAG ACG CAA GGC CGA TAT GTT GTT 2016 Ile Thr Asn AlaGln Leu Phe Ser Glu Thr Gln Gly Arg Tyr Val Val 660 665 670 TCT GTT AAATCA GGT AAA ACT TTA AAT ATT GAT AAT GCA ATA GAA ATT 2064 Ser Val Lys SerGly Lys Thr Leu Asn Ile Asp Asn Ala Ile Glu Ile 675 680 685 GGA CTT TTAACA GAT AGT GAT AAT TYC AAG GTA ACA ACA CCA TAT ACA 2112 Gly Leu Leu ThrAsp Ser Asp Asn Xaa Lys Val Thr Thr Pro Tyr Thr 690 695 700 GAG ATT AGTGAA AAT GTT TCA GAT ATT AAA CAA ATA TGG GAA GGG GCA 2160 Glu Ile Ser GluAsn Val Ser Asp Ile Lys Gln Ile Trp Glu Gly Ala 705 710 715 720 ATT GCTCAA TGT TTA ACT ACT CAG GAT TAA 2190 Ile Ala Gln Cys Leu Thr Thr Gln Asp725 729 amino acids amino acid linear protein 30 Met Ser Lys Phe Ile GluPro Ser Val Glu Glu Ile Lys Leu Glu Lys 1 5 10 15 Val Tyr Gln Asp MetGly Leu Ser Asp Gln Glu Tyr Glu Lys Val Cys 20 25 30 Asp Ile Leu Gly ArgGln Pro Asn Phe Thr Glu Thr Gly Ile Phe Ser 35 40 45 Val Met Trp Ser GluHis Cys Ser Tyr Lys His Ser Lys Pro Phe Leu 50 55 60 Lys Gln Phe Pro ThrSer Gly Asp His Val Leu Met Gly Pro Gly Glu 65 70 75 80 Gly Ala Gly ValVal Asp Ile Gly Asp Asn Gln Ala Val Val Phe Lys 85 90 95 Val Glu Ser HisAsn His Pro Ser Ala Ile Glu Pro Tyr Gln Gly Ala 100 105 110 Ala Thr GlyVal Gly Gly Ile Ile Arg Asp Ile Val Ser Ile Gly Ala 115 120 125 Arg ProIle Asn Leu Leu Asn Ser Leu Arg Phe Gly Glu Leu Asp Asn 130 135 140 LysGln Asn Gln Arg Leu Leu Lys Gly Val Val Lys Gly Ile Gly Gly 145 150 155160 Tyr Gly Asn Cys Ile Gly Ile Pro Thr Thr Ala Gly Glu Ile Glu Phe 165170 175 Asp Glu Arg Tyr Asp Gly Asn Pro Leu Val Asn Ala Met Cys Val Gly180 185 190 Val Ile Asn His Asp Met Ile Gln Lys Gly Thr Ala Lys Gly ValGly 195 200 205 Asn Ser Val Ile Tyr Val Gly Leu Lys Thr Gly Arg Asp GlyIle His 210 215 220 Gly Ala Thr Phe Ala Ser Glu Glu Leu Thr Glu Glu SerGlu Ser Lys 225 230 235 240 Arg Pro Ser Val Gln Ile Gly Asp Pro Phe ValGly Lys Lys Leu Met 245 250 255 Glu Ala Thr Leu Glu Ala Ile Thr Phe AspGlu Leu Val Gly Ile Gln 260 265 270 Asp Met Gly Ala Ala Gly Leu Thr SerSer Ser Ser Glu Met Ala Ala 275 280 285 Lys Gly Gly Ser Gly Leu His LeuArg Leu Glu Gln Val Pro Thr Arg 290 295 300 Glu Pro Gly Ile Ser Pro TyrGlu Met Met Leu Ser Glu Thr Gln Glu 305 310 315 320 Arg Met Leu Leu ValVal Glu Lys Gly Thr Glu Gln Lys Phe Leu Asp 325 330 335 Leu Phe Asp LysHis Glu Leu Asp Ser Ala Val Ile Gly Glu Val Thr 340 345 350 Asp Thr AsnArg Phe Val Leu Thr Tyr Asp Asp Glu Val Tyr Ala Asp 355 360 365 Ile ProVal Glu Pro Leu Ala Asp Glu Ala Pro Val Tyr Ile Leu Glu 370 375 380 GlyGlu Glu Lys Asp Tyr Asn Thr Ser Lys Asn Asp Tyr Thr His Ile 385 390 395400 Asp Val Lys Asp Thr Phe Phe Lys Leu Leu Lys His Pro Thr Ile Ala 405410 415 Ser Lys His Tyr Leu Tyr Asp Gln Tyr Asp Gln Gln Val Gly Ala Asn420 425 430 Thr Ile Ile Lys Pro Gly Leu Gln Ala Ser Val Val Arg Val GluGly 435 440 445 Thr Asn Lys Ala Ile Ala Ser Thr Ile Asp Gly Glu Ala ArgTyr Val 450 455 460 Tyr Asn Asn Pro Tyr Glu Gly Gly Lys Met Val Val AlaGlu Ala Tyr 465 470 475 480 Arg Asn Leu Ile Ala Val Gly Ala Thr Pro LeuAla Met Thr Asp Cys 485 490 495 Leu Asn Tyr Gly Ser Pro Glu Lys Lys GluIle Tyr Gln Gln Leu Ile 500 505 510 Asp Ser Thr Lys Gly Met Ala Glu AlaCys Asp Ile Leu Lys Thr Pro 515 520 525 Val Val Ser Gly Asn Val Ser LeuTyr Asn Glu Thr Lys Gly Thr Ser 530 535 540 Ile Phe Pro Thr Pro Val ValGly Met Val Gly Leu Ile Glu Asn Val 545 550 555 560 Asn Tyr Leu Asn AspPhe Glu Pro Gln Val Gly Asp Lys Leu Tyr Leu 565 570 575 Ile Gly Asp ThrLys Asp Asp Phe Gly Gly Ser Gln Leu Glu Lys Leu 580 585 590 Ile Tyr GlyLys Val Asn His Glu Phe Glu Ser Leu Asp Leu Ser Ser 595 600 605 Glu ValGlu Lys Gly Glu Ser Ile Lys Thr Ala Ile Arg Glu Gly Leu 610 615 620 LeuSer His Val Gln Thr Val Gly Lys Gly Gly Leu Leu Ile Thr Leu 625 630 635640 Ala Lys Leu Ser Ala His Tyr Gly Leu Gly Leu Lys Ser Ser Ile Asp 645650 655 Ile Thr Asn Ala Gln Leu Phe Ser Glu Thr Gln Gly Arg Tyr Val Val660 665 670 Ser Val Lys Ser Gly Lys Thr Leu Asn Ile Asp Asn Ala Ile GluIle 675 680 685 Gly Leu Leu Thr Asp Ser Asp Asn Xaa Lys Val Thr Thr ProTyr Thr 690 695 700 Glu Ile Ser Glu Asn Val Ser Asp Ile Lys Gln Ile TrpGlu Gly Ala 705 710 715 720 Ile Ala Gln Cys Leu Thr Thr Gln Asp 725 729base pairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p10b18/p6b18)“ CDS 1..726 31 ATG ACT AAA TTA TTT ATA CCT TATATT ATG GGC AAT AAA GAT TTG ATT 48 Met Thr Lys Leu Phe Ile Pro Tyr IleMet Gly Asn Lys Asp Leu Ile 1 5 10 15 GAA AAT GCA ACA TTG TTG AGT GAAAAT GGT GCA GAT ATA ATT GAA ATT 96 Glu Asn Ala Thr Leu Leu Ser Glu AsnGly Ala Asp Ile Ile Glu Ile 20 25 30 GGA GTA CCT TTC TCT GAT CCG GTT GCTGAT GGT CCA GTT ATC ATG GAA 144 Gly Val Pro Phe Ser Asp Pro Val Ala AspGly Pro Val Ile Met Glu 35 40 45 GCA GGT CAA CAA GCG ATT AAA CAA GGC ATCACG ATA GAT TAT ATT TTC 192 Ala Gly Gln Gln Ala Ile Lys Gln Gly Ile ThrIle Asp Tyr Ile Phe 50 55 60 AAT CAA TTA GAA AAA CAT GGT GAT CAA ATT AAGTGT AAC TAT GTA TTA 240 Asn Gln Leu Glu Lys His Gly Asp Gln Ile Lys CysAsn Tyr Val Leu 65 70 75 80 ATG ACG TAT TAT AAT ATT ATT TGT CAT TAT GGAGAA CAA GCG TTT TTT 288 Met Thr Tyr Tyr Asn Ile Ile Cys His Tyr Gly GluGln Ala Phe Phe 85 90 95 GAA AAA TGT CGA GAT ACT GGT GTC TAC GGC TTA ATTATT CCT GAT TTA 336 Glu Lys Cys Arg Asp Thr Gly Val Tyr Gly Leu Ile IlePro Asp Leu 100 105 110 CCA TAT GAA TTA TCG CAG CGT TTA AAA CAA CAA TTTAGT CAC TAT GGC 384 Pro Tyr Glu Leu Ser Gln Arg Leu Lys Gln Gln Phe SerHis Tyr Gly 115 120 125 GTC AAA ATC ATA TCG TTA GTT GCG ATG ACT ACT GATGAC AAA CGT ATA 432 Val Lys Ile Ile Ser Leu Val Ala Met Thr Thr Asp AspLys Arg Ile 130 135 140 AAA GAT ATC GTA TCC CAT GCG GAA GGC TTT ATT TATACT GTG ACG ATG 480 Lys Asp Ile Val Ser His Ala Glu Gly Phe Ile Tyr ThrVal Thr Met 145 150 155 160 AAT GCG ACA ACA GGG CAA AAC GGT GCG TTT CATCCA GAA TTA AAA CGA 528 Asn Ala Thr Thr Gly Gln Asn Gly Ala Phe His ProGlu Leu Lys Arg 165 170 175 AAA ATT GAG TCA ATT AAA GCG ATA GCC AAT GTGCCA GTT GTC GCA GGA 576 Lys Ile Glu Ser Ile Lys Ala Ile Ala Asn Val ProVal Val Ala Gly 180 185 190 TTT GGT ATA AGA ACA CCA CAA CAT GTT GCA GATATA AAA GAG GTT GCA 624 Phe Gly Ile Arg Thr Pro Gln His Val Ala Asp IleLys Glu Val Ala 195 200 205 GAT GGC ATT GTC ATT GGT AGC GAA ATC GTT AAGCGA TTT AAA TCT AAC 672 Asp Gly Ile Val Ile Gly Ser Glu Ile Val Lys ArgPhe Lys Ser Asn 210 215 220 ACG CGT GAG GAA ATC ATT AAA TAT TTA CAA TCTATC CAA CAA ACA TTG 720 Thr Arg Glu Glu Ile Ile Lys Tyr Leu Gln Ser IleGln Gln Thr Leu 225 230 235 240 AAT AAT TAA 729 Asn Asn 242 amino acidsamino acid linear protein 32 Met Thr Lys Leu Phe Ile Pro Tyr Ile Met GlyAsn Lys Asp Leu Ile 1 5 10 15 Glu Asn Ala Thr Leu Leu Ser Glu Asn GlyAla Asp Ile Ile Glu Ile 20 25 30 Gly Val Pro Phe Ser Asp Pro Val Ala AspGly Pro Val Ile Met Glu 35 40 45 Ala Gly Gln Gln Ala Ile Lys Gln Gly IleThr Ile Asp Tyr Ile Phe 50 55 60 Asn Gln Leu Glu Lys His Gly Asp Gln IleLys Cys Asn Tyr Val Leu 65 70 75 80 Met Thr Tyr Tyr Asn Ile Ile Cys HisTyr Gly Glu Gln Ala Phe Phe 85 90 95 Glu Lys Cys Arg Asp Thr Gly Val TyrGly Leu Ile Ile Pro Asp Leu 100 105 110 Pro Tyr Glu Leu Ser Gln Arg LeuLys Gln Gln Phe Ser His Tyr Gly 115 120 125 Val Lys Ile Ile Ser Leu ValAla Met Thr Thr Asp Asp Lys Arg Ile 130 135 140 Lys Asp Ile Val Ser HisAla Glu Gly Phe Ile Tyr Thr Val Thr Met 145 150 155 160 Asn Ala Thr ThrGly Gln Asn Gly Ala Phe His Pro Glu Leu Lys Arg 165 170 175 Lys Ile GluSer Ile Lys Ala Ile Ala Asn Val Pro Val Val Ala Gly 180 185 190 Phe GlyIle Arg Thr Pro Gln His Val Ala Asp Ile Lys Glu Val Ala 195 200 205 AspGly Ile Val Ile Gly Ser Glu Ile Val Lys Arg Phe Lys Ser Asn 210 215 220Thr Arg Glu Glu Ile Ile Lys Tyr Leu Gln Ser Ile Gln Gln Thr Leu 225 230235 240 Asn Asn 1215 base pairs nucleic acid single linear other nucleicacid /desc = ”DNA (genomic) (p10b66)“ CDS 1..1212 33 ATG AAT AAA CAA ATACAA ACA GAA GCA GAT GAA TTA GGT TTC TTT GGT 48 Met Asn Lys Gln Ile GlnThr Glu Ala Asp Glu Leu Gly Phe Phe Gly 1 5 10 15 GAA TAT GGA GGG CAATAT GTT CCA GAA ACA TTA ATG CCA GCA ATT ATT 96 Glu Tyr Gly Gly Gln TyrVal Pro Glu Thr Leu Met Pro Ala Ile Ile 20 25 30 GAG TTG AAA AAA GCT TATAAA GAG GCA AAA GCA GAC CCA GAG TTT CAA 144 Glu Leu Lys Lys Ala Tyr LysGlu Ala Lys Ala Asp Pro Glu Phe Gln 35 40 45 AGA GAA CTG GAA TAC TAT TTATCA GAG TAT GTA GGA CGC GCG ACA CCA 192 Arg Glu Leu Glu Tyr Tyr Leu SerGlu Tyr Val Gly Arg Ala Thr Pro 50 55 60 CTT ACA TAT GCT GCA TCA TAT ACTGAA AGC CTA GGT GGC GCT AAA ATA 240 Leu Thr Tyr Ala Ala Ser Tyr Thr GluSer Leu Gly Gly Ala Lys Ile 65 70 75 80 TAT TTG AAA CGA GAG GAT CTA AATCAT ACA GGC GCC CAT AAA ATT AAT 288 Tyr Leu Lys Arg Glu Asp Leu Asn HisThr Gly Ala His Lys Ile Asn 85 90 95 AAT GCG TTA GGT CAA GCG TTG CTT GCTAAA AGA ATG GGC AAG AAG AAG 336 Asn Ala Leu Gly Gln Ala Leu Leu Ala LysArg Met Gly Lys Lys Lys 100 105 110 CTT GTT GCT GAA ACT GGT GCG GGT CAACAT GGT GTA GCT AGT GCT ACG 384 Leu Val Ala Glu Thr Gly Ala Gly Gln HisGly Val Ala Ser Ala Thr 115 120 125 GTT GCT GCA TTA TTT GAT ATG GAA CTTGTT GTC TTT ATG GGA AGT GAA 432 Val Ala Ala Leu Phe Asp Met Glu Leu ValVal Phe Met Gly Ser Glu 130 135 140 GAT ATT AAA AGA CAA CAA CTT AAT GTATTT AGA ATG GAA TTA CTT GGT 480 Asp Ile Lys Arg Gln Gln Leu Asn Val PheArg Met Glu Leu Leu Gly 145 150 155 160 GCA AAG GTT GTG GCA GTT GAA GATGGT CAA GGG ACT TTA TCG GAT GCA 528 Ala Lys Val Val Ala Val Glu Asp GlyGln Gly Thr Leu Ser Asp Ala 165 170 175 GTT AAT AAA GCA TTG CAA TAT TGGGTA AGT CAT GTA GAT GAT ACA CAT 576 Val Asn Lys Ala Leu Gln Tyr Trp ValSer His Val Asp Asp Thr His 180 185 190 TAT TTA TTA GGT TCT GCA TTA GGTCCA GAC CCG TTC CCA ACG ATT GTT 624 Tyr Leu Leu Gly Ser Ala Leu Gly ProAsp Pro Phe Pro Thr Ile Val 195 200 205 AGA GAT TTT CAG AGT GTG ATT GGTAAA GAA ATA AAA TCA CAG ATA TTG 672 Arg Asp Phe Gln Ser Val Ile Gly LysGlu Ile Lys Ser Gln Ile Leu 210 215 220 AAG AAA GAA GGT CGA CTT CCG GATGCA ATT GTA GCA TGT ATC GGT GGT 720 Lys Lys Glu Gly Arg Leu Pro Asp AlaIle Val Ala Cys Ile Gly Gly 225 230 235 240 GGC TCA AAT GCA ATC GGT ACATTT TAT CCA TTT ATT AAA GAT GAT GTT 768 Gly Ser Asn Ala Ile Gly Thr PheTyr Pro Phe Ile Lys Asp Asp Val 245 250 255 GCA TTA TAC GGT GTT GAA GCCGCA GGT CAA GGC GAT GAT ACT GAT AAA 816 Ala Leu Tyr Gly Val Glu Ala AlaGly Gln Gly Asp Asp Thr Asp Lys 260 265 270 CAT GCA CTT GCA ATT GGC AAAGGA TCA CCT GGC GTA TTA CAT GGT ACT 864 His Ala Leu Ala Ile Gly Lys GlySer Pro Gly Val Leu His Gly Thr 275 280 285 AAA ATG TAT TTA ATT CAA GATGAA GAT GGG CAA GTG CAA CTA GCA CAT 912 Lys Met Tyr Leu Ile Gln Asp GluAsp Gly Gln Val Gln Leu Ala His 290 295 300 TCT ATT TCA GCA GGA CTT GATTAT CCT GGT ATT GGA CCA GAA CAT TCT 960 Ser Ile Ser Ala Gly Leu Asp TyrPro Gly Ile Gly Pro Glu His Ser 305 310 315 320 TAT TAC CAC GAC ATT GGTAGA GTA ACT TTT GAA AAT GCT AGT GAT ACA 1008 Tyr Tyr His Asp Ile Gly ArgVal Thr Phe Glu Asn Ala Ser Asp Thr 325 330 335 CAA GCA ATG AAT GCT TTAATC AAC TTT ACA AAA CAT GAA GGT ATT ATA 1056 Gln Ala Met Asn Ala Leu IleAsn Phe Thr Lys His Glu Gly Ile Ile 340 345 350 CCT GCA ATT GAA AGT GCACAT GCA CTG AGT TAT GTT GAA AGA CTA GCG 1104 Pro Ala Ile Glu Ser Ala HisAla Leu Ser Tyr Val Glu Arg Leu Ala 355 360 365 CCT ACG ATG TCG AAA GAAGAT ATT ATT GTA GTA ACT ATT TCT GGA CGT 1152 Pro Thr Met Ser Lys Glu AspIle Ile Val Val Thr Ile Ser Gly Arg 370 375 380 GGC GAT AAA GAT ATG GAAACA ATT AGA CAA TAT ATG GTA GAG CGA GGT 1200 Gly Asp Lys Asp Met Glu ThrIle Arg Gln Tyr Met Val Glu Arg Gly 385 390 395 400 CTT GCA AAT GAC TAA1215 Leu Ala Asn Asp 404 amino acids amino acid linear protein 34 MetAsn Lys Gln Ile Gln Thr Glu Ala Asp Glu Leu Gly Phe Phe Gly 1 5 10 15Glu Tyr Gly Gly Gln Tyr Val Pro Glu Thr Leu Met Pro Ala Ile Ile 20 25 30Glu Leu Lys Lys Ala Tyr Lys Glu Ala Lys Ala Asp Pro Glu Phe Gln 35 40 45Arg Glu Leu Glu Tyr Tyr Leu Ser Glu Tyr Val Gly Arg Ala Thr Pro 50 55 60Leu Thr Tyr Ala Ala Ser Tyr Thr Glu Ser Leu Gly Gly Ala Lys Ile 65 70 7580 Tyr Leu Lys Arg Glu Asp Leu Asn His Thr Gly Ala His Lys Ile Asn 85 9095 Asn Ala Leu Gly Gln Ala Leu Leu Ala Lys Arg Met Gly Lys Lys Lys 100105 110 Leu Val Ala Glu Thr Gly Ala Gly Gln His Gly Val Ala Ser Ala Thr115 120 125 Val Ala Ala Leu Phe Asp Met Glu Leu Val Val Phe Met Gly SerGlu 130 135 140 Asp Ile Lys Arg Gln Gln Leu Asn Val Phe Arg Met Glu LeuLeu Gly 145 150 155 160 Ala Lys Val Val Ala Val Glu Asp Gly Gln Gly ThrLeu Ser Asp Ala 165 170 175 Val Asn Lys Ala Leu Gln Tyr Trp Val Ser HisVal Asp Asp Thr His 180 185 190 Tyr Leu Leu Gly Ser Ala Leu Gly Pro AspPro Phe Pro Thr Ile Val 195 200 205 Arg Asp Phe Gln Ser Val Ile Gly LysGlu Ile Lys Ser Gln Ile Leu 210 215 220 Lys Lys Glu Gly Arg Leu Pro AspAla Ile Val Ala Cys Ile Gly Gly 225 230 235 240 Gly Ser Asn Ala Ile GlyThr Phe Tyr Pro Phe Ile Lys Asp Asp Val 245 250 255 Ala Leu Tyr Gly ValGlu Ala Ala Gly Gln Gly Asp Asp Thr Asp Lys 260 265 270 His Ala Leu AlaIle Gly Lys Gly Ser Pro Gly Val Leu His Gly Thr 275 280 285 Lys Met TyrLeu Ile Gln Asp Glu Asp Gly Gln Val Gln Leu Ala His 290 295 300 Ser IleSer Ala Gly Leu Asp Tyr Pro Gly Ile Gly Pro Glu His Ser 305 310 315 320Tyr Tyr His Asp Ile Gly Arg Val Thr Phe Glu Asn Ala Ser Asp Thr 325 330335 Gln Ala Met Asn Ala Leu Ile Asn Phe Thr Lys His Glu Gly Ile Ile 340345 350 Pro Ala Ile Glu Ser Ala His Ala Leu Ser Tyr Val Glu Arg Leu Ala355 360 365 Pro Thr Met Ser Lys Glu Asp Ile Ile Val Val Thr Ile Ser GlyArg 370 375 380 Gly Asp Lys Asp Met Glu Thr Ile Arg Gln Tyr Met Val GluArg Gly 385 390 395 400 Leu Ala Asn Asp 1008 base pairs nucleic acidsingle linear other nucleic acid /desc = ”DNA (genomic) (p10c34)“ CDS1..1005 35 ATG AAA ACC ATG ACA TTA CTA ACA AGA ATA AAA ACT GAA ACT ATATTA 48 Met Lys Thr Met Thr Leu Leu Thr Arg Ile Lys Thr Glu Thr Ile Leu 15 10 15 CTT GAA AGC GAC ATT AAA GAG CTA ATC GAT ATA CTT ATT TCT CCT AGT96 Leu Glu Ser Asp Ile Lys Glu Leu Ile Asp Ile Leu Ile Ser Pro Ser 20 2530 ATT GGA ACT GAT ATT AAA TAT GAA TTA CTT AGT TCC TAT TCG GAG CGA 144Ile Gly Thr Asp Ile Lys Tyr Glu Leu Leu Ser Ser Tyr Ser Glu Arg 35 40 45GAA ATC CAA CAA CAA GAA TTA ACA TAT ATT GTA CGT AGC TTA ATT AAT 192 GluIle Gln Gln Gln Glu Leu Thr Tyr Ile Val Arg Ser Leu Ile Asn 50 55 60 ACAATG TAT CCA CAT CAA CCA TGT TAT GAA GGG GCT ATG TGT GTG TGC 240 Thr MetTyr Pro His Gln Pro Cys Tyr Glu Gly Ala Met Cys Val Cys 65 70 75 80 GGCACA GGT GGT GAC AAG TCA AAT AGT TTC AAC ATT TCA ACG ACT GTT 288 Gly ThrGly Gly Asp Lys Ser Asn Ser Phe Asn Ile Ser Thr Thr Val 85 90 95 GCT TTTGTT GTA GCA AGT GCT GGC GTA AAA GTT ATA AAA CAT GGT AAT 336 Ala Phe ValVal Ala Ser Ala Gly Val Lys Val Ile Lys His Gly Asn 100 105 110 AAA AGTATT ACC TCA AAT TCA GGT AGT ACG GAT TTG TTA AAT CAA ATG 384 Lys Ser IleThr Ser Asn Ser Gly Ser Thr Asp Leu Leu Asn Gln Met 115 120 125 AAC ATACAA ACA ACA ACT GTT GAT GAT ACA CCT AAC CAA TTA AAT GAN 432 Asn Ile GlnThr Thr Thr Val Asp Asp Thr Pro Asn Gln Leu Asn Xaa 130 135 140 AAA GACCTT GTA TTC ATT GGT GCA ACT GAA TCA TAT CCA ATC ATG AAG 480 Lys Asp LeuVal Phe Ile Gly Ala Thr Glu Ser Tyr Pro Ile Met Lys 145 150 155 160 TATATG CAA CCA GTT AGA AAA ATG ATT GGA AAG CCT ACA ATA TTA AAC 528 Tyr MetGln Pro Val Arg Lys Met Ile Gly Lys Pro Thr Ile Leu Asn 165 170 175 CTTGTG GGT CCA TTA ATT AAT CCA TAT CAC TTA ACG TAT CAA ATG GTA 576 Leu ValGly Pro Leu Ile Asn Pro Tyr His Leu Thr Tyr Gln Met Val 180 185 190 GGCGTC TTT GAT CCT ACA AAG TTA AAG TTA GTT GCT AAA ACG ATT AAA 624 Gly ValPhe Asp Pro Thr Lys Leu Lys Leu Val Ala Lys Thr Ile Lys 195 200 205 GATTTA GGT AGA AAA CGT GCA ATC GTT TTA CAT GGT GCA AAT GGT ATG 672 Asp LeuGly Arg Lys Arg Ala Ile Val Leu His Gly Ala Asn Gly Met 210 215 220 GATGAA GCA ACA CTA TCT GGT GAT AAT TTG ATA TAT GAA TTG ACT GAA 720 Asp GluAla Thr Leu Ser Gly Asp Asn Leu Ile Tyr Glu Leu Thr Glu 225 230 235 240GAT GGA GAA ATC AAA AAT TAC ACA TTA AAT GCG ACT GAT TAT GGT TTG 768 AspGly Glu Ile Lys Asn Tyr Thr Leu Asn Ala Thr Asp Tyr Gly Leu 245 250 255AAA CAT GCG CCG AAT AGT GAT TTT AAA GGC GGT TCA CCT GAA GAA AAT 816 LysHis Ala Pro Asn Ser Asp Phe Lys Gly Gly Ser Pro Glu Glu Asn 260 265 270TTA GCA ATC TCC CTT AAT ATC TTG AAT GGT AAA GAT CAG TCA RGT CGA 864 LeuAla Ile Ser Leu Asn Ile Leu Asn Gly Lys Asp Gln Ser Xaa Arg 275 280 285CGT GAT GTT GTC TTA CTA AAT GCG GGT TTA AGC CTT TAT GTT GCA GAG 912 ArgAsp Val Val Leu Leu Asn Ala Gly Leu Ser Leu Tyr Val Ala Glu 290 295 300AAA RTG GAT ACC ATC GCA GAA GGC ATA GAA CTT GCA ACT ACA TTG ATT 960 LysXaa Asp Thr Ile Ala Glu Gly Ile Glu Leu Ala Thr Thr Leu Ile 305 310 315320 GAT AAT GGT GAA GCA TTG GAA AAA TAC CAT CAA ATG AGA GGT GAA 1005 AspAsn Gly Glu Ala Leu Glu Lys Tyr His Gln Met Arg Gly Glu 325 330 335 TAA1008 335 amino acids amino acid linear protein 36 Met Lys Thr Met ThrLeu Leu Thr Arg Ile Lys Thr Glu Thr Ile Leu 1 5 10 15 Leu Glu Ser AspIle Lys Glu Leu Ile Asp Ile Leu Ile Ser Pro Ser 20 25 30 Ile Gly Thr AspIle Lys Tyr Glu Leu Leu Ser Ser Tyr Ser Glu Arg 35 40 45 Glu Ile Gln GlnGln Glu Leu Thr Tyr Ile Val Arg Ser Leu Ile Asn 50 55 60 Thr Met Tyr ProHis Gln Pro Cys Tyr Glu Gly Ala Met Cys Val Cys 65 70 75 80 Gly Thr GlyGly Asp Lys Ser Asn Ser Phe Asn Ile Ser Thr Thr Val 85 90 95 Ala Phe ValVal Ala Ser Ala Gly Val Lys Val Ile Lys His Gly Asn 100 105 110 Lys SerIle Thr Ser Asn Ser Gly Ser Thr Asp Leu Leu Asn Gln Met 115 120 125 AsnIle Gln Thr Thr Thr Val Asp Asp Thr Pro Asn Gln Leu Asn Xaa 130 135 140Lys Asp Leu Val Phe Ile Gly Ala Thr Glu Ser Tyr Pro Ile Met Lys 145 150155 160 Tyr Met Gln Pro Val Arg Lys Met Ile Gly Lys Pro Thr Ile Leu Asn165 170 175 Leu Val Gly Pro Leu Ile Asn Pro Tyr His Leu Thr Tyr Gln MetVal 180 185 190 Gly Val Phe Asp Pro Thr Lys Leu Lys Leu Val Ala Lys ThrIle Lys 195 200 205 Asp Leu Gly Arg Lys Arg Ala Ile Val Leu His Gly AlaAsn Gly Met 210 215 220 Asp Glu Ala Thr Leu Ser Gly Asp Asn Leu Ile TyrGlu Leu Thr Glu 225 230 235 240 Asp Gly Glu Ile Lys Asn Tyr Thr Leu AsnAla Thr Asp Tyr Gly Leu 245 250 255 Lys His Ala Pro Asn Ser Asp Phe LysGly Gly Ser Pro Glu Glu Asn 260 265 270 Leu Ala Ile Ser Leu Asn Ile LeuAsn Gly Lys Asp Gln Ser Xaa Arg 275 280 285 Arg Asp Val Val Leu Leu AsnAla Gly Leu Ser Leu Tyr Val Ala Glu 290 295 300 Lys Xaa Asp Thr Ile AlaGlu Gly Ile Glu Leu Ala Thr Thr Leu Ile 305 310 315 320 Asp Asn Gly GluAla Leu Glu Lys Tyr His Gln Met Arg Gly Glu 325 330 335 1254 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p4c27)“ CDS 1..1251 37 ATG GCG GAA ATT AAG GTA CCT GAA TTA GCA GAA TCAATC TCA GAA GGA 48 Met Ala Glu Ile Lys Val Pro Glu Leu Ala Glu Ser IleSer Glu Gly 1 5 10 15 ACA ATA GCC CAA TGG TTA AAG CAG CCT GGT GAC TATGTA GAA CAG GGT 96 Thr Ile Ala Gln Trp Leu Lys Gln Pro Gly Asp Tyr ValGlu Gln Gly 20 25 30 GAA TAT CTG CTT GAA CTA GAA ACG GAT AAA GTG AAT GTTGAA TTG ACA 144 Glu Tyr Leu Leu Glu Leu Glu Thr Asp Lys Val Asn Val GluLeu Thr 35 40 45 GCA GAA GAA TCG GGT GTA CTT CAA GAG GTA TTG AAA GAT TCGGGT GAT 192 Ala Glu Glu Ser Gly Val Leu Gln Glu Val Leu Lys Asp Ser GlyAsp 50 55 60 ACC GTC CAG GTC GGA GAA ATT ATC GGT ACG ATT TCA GAA GGC GCGGGT 240 Thr Val Gln Val Gly Glu Ile Ile Gly Thr Ile Ser Glu Gly Ala Gly65 70 75 80 GAA AGT TCT GCC CCT GCT CCT ACA GAG AAA ACA GAA AGC AAG GAAAGC 288 Glu Ser Ser Ala Pro Ala Pro Thr Glu Lys Thr Glu Ser Lys Glu Ser85 90 95 GTA AAA GAA GAG AAA CAG GCT GAA CCA GCT GCA CAA GAG GTG AGC GAG336 Val Lys Glu Glu Lys Gln Ala Glu Pro Ala Ala Gln Glu Val Ser Glu 100105 110 GAA GCA CAA TCT GAA GCA AAA TCA AGA ACG ATC GCT TCT CCG TCG GCC384 Glu Ala Gln Ser Glu Ala Lys Ser Arg Thr Ile Ala Ser Pro Ser Ala 115120 125 CGT AAG CTT GCG AGA GAA AAA GGA ATT GAC CTG TCT CAA GTT CCA ACT432 Arg Lys Leu Ala Arg Glu Lys Gly Ile Asp Leu Ser Gln Val Pro Thr 130135 140 GGA GAT CCG CTT GGA AGA GTG CGC AAG CAG GAT GTC GAA GCG TAC GAA480 Gly Asp Pro Leu Gly Arg Val Arg Lys Gln Asp Val Glu Ala Tyr Glu 145150 155 160 AAA CCG GCA TCA AAA CCG GCT CCT CAG CAA AAG CAG CAG CCT CAGGCT 528 Lys Pro Ala Ser Lys Pro Ala Pro Gln Gln Lys Gln Gln Pro Gln Ala165 170 175 CAA AAA GCA CAG CAA AGC TTT GAC AAA CCT GTT GAA GTG CAA AAAATG 576 Gln Lys Ala Gln Gln Ser Phe Asp Lys Pro Val Glu Val Gln Lys Met180 185 190 TCA CGC CGC AGA CAA ACG ATT GCA AAA CGC CTT GTA GAG GTA CAGCAA 624 Ser Arg Arg Arg Gln Thr Ile Ala Lys Arg Leu Val Glu Val Gln Gln195 200 205 ACA TCA GCG ATG CTG ACT ACA TTT AAT GAA GTG GAC ATG ACG GCTGTC 672 Thr Ser Ala Met Leu Thr Thr Phe Asn Glu Val Asp Met Thr Ala Val210 215 220 ATG AAT CTC AGA AAA CGC CGC AAA GAT CAA TTT TTT GAG CAA AATGAA 720 Met Asn Leu Arg Lys Arg Arg Lys Asp Gln Phe Phe Glu Gln Asn Glu225 230 235 240 GTG AAG CTC GGC TTT ATG TCT TTC TTC ACG AAA GCG GTC GTGGCT GCA 768 Val Lys Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val AlaAla 245 250 255 TTG AAA AAA TAT CCG CTG TTG AAT GCA GAA ATT CAA GGC GATGAG TTG 816 Leu Lys Lys Tyr Pro Leu Leu Asn Ala Glu Ile Gln Gly Asp GluLeu 260 265 270 ATC GTT AAA AAA TTC TAC GAT ATC GGA ATC GCT GTT GCT GCTGTA GAA 864 Ile Val Lys Lys Phe Tyr Asp Ile Gly Ile Ala Val Ala Ala ValGlu 275 280 285 GGT CTT GTC GTT CCG GTT GTA CGG GAT GCG GAT CGC CTG ACATTT GCA 912 Gly Leu Val Val Pro Val Val Arg Asp Ala Asp Arg Leu Thr PheAla 290 295 300 GGA ATC GAA AAA GAG ATC GGC GAG CTT GCG AAA AAA GCA AGAAAC AAT 960 Gly Ile Glu Lys Glu Ile Gly Glu Leu Ala Lys Lys Ala Arg AsnAsn 305 310 315 320 AAA TTA ACC CTT AGC GAG CTT GAG GGA GGC TCC TTC ACGATT ACA AAC 1008 Lys Leu Thr Leu Ser Glu Leu Glu Gly Gly Ser Phe Thr IleThr Asn 325 330 335 GGA GGG ACT TTC GGT TCA TTG ATG TCA ACT CCA ATT TTAAAC AGC CCG 1056 Gly Gly Thr Phe Gly Ser Leu Met Ser Thr Pro Ile Leu AsnSer Pro 340 345 350 CAA GTC GGT ATA CTG GGC ATG CAT AAG ATT CAG CTG CGCCCT GTA GCC 1104 Gln Val Gly Ile Leu Gly Met His Lys Ile Gln Leu Arg ProVal Ala 355 360 365 ATT GAT GAA GAG CGT TTC GAA AAC CGT CCG ATG ATG TATATC GCT TTA 1152 Ile Asp Glu Glu Arg Phe Glu Asn Arg Pro Met Met Tyr IleAla Leu 370 375 380 TCT TAT GAT CAC CGA ATT GTA GAC GGT AAA GAA GCG GTTGGT TTC CTC 1200 Ser Tyr Asp His Arg Ile Val Asp Gly Lys Glu Ala Val GlyPhe Leu 385 390 395 400 GTG ACA ATC AAA AAT TTA CTG GAA GAT CCT GAA CAGCTT TTA TTA GAA 1248 Val Thr Ile Lys Asn Leu Leu Glu Asp Pro Glu Gln LeuLeu Leu Glu 405 410 415 GGA TAA 1254 Gly 417 amino acids amino acidlinear protein 38 Met Ala Glu Ile Lys Val Pro Glu Leu Ala Glu Ser IleSer Glu Gly 1 5 10 15 Thr Ile Ala Gln Trp Leu Lys Gln Pro Gly Asp TyrVal Glu Gln Gly 20 25 30 Glu Tyr Leu Leu Glu Leu Glu Thr Asp Lys Val AsnVal Glu Leu Thr 35 40 45 Ala Glu Glu Ser Gly Val Leu Gln Glu Val Leu LysAsp Ser Gly Asp 50 55 60 Thr Val Gln Val Gly Glu Ile Ile Gly Thr Ile SerGlu Gly Ala Gly 65 70 75 80 Glu Ser Ser Ala Pro Ala Pro Thr Glu Lys ThrGlu Ser Lys Glu Ser 85 90 95 Val Lys Glu Glu Lys Gln Ala Glu Pro Ala AlaGln Glu Val Ser Glu 100 105 110 Glu Ala Gln Ser Glu Ala Lys Ser Arg ThrIle Ala Ser Pro Ser Ala 115 120 125 Arg Lys Leu Ala Arg Glu Lys Gly IleAsp Leu Ser Gln Val Pro Thr 130 135 140 Gly Asp Pro Leu Gly Arg Val ArgLys Gln Asp Val Glu Ala Tyr Glu 145 150 155 160 Lys Pro Ala Ser Lys ProAla Pro Gln Gln Lys Gln Gln Pro Gln Ala 165 170 175 Gln Lys Ala Gln GlnSer Phe Asp Lys Pro Val Glu Val Gln Lys Met 180 185 190 Ser Arg Arg ArgGln Thr Ile Ala Lys Arg Leu Val Glu Val Gln Gln 195 200 205 Thr Ser AlaMet Leu Thr Thr Phe Asn Glu Val Asp Met Thr Ala Val 210 215 220 Met AsnLeu Arg Lys Arg Arg Lys Asp Gln Phe Phe Glu Gln Asn Glu 225 230 235 240Val Lys Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val Ala Ala 245 250255 Leu Lys Lys Tyr Pro Leu Leu Asn Ala Glu Ile Gln Gly Asp Glu Leu 260265 270 Ile Val Lys Lys Phe Tyr Asp Ile Gly Ile Ala Val Ala Ala Val Glu275 280 285 Gly Leu Val Val Pro Val Val Arg Asp Ala Asp Arg Leu Thr PheAla 290 295 300 Gly Ile Glu Lys Glu Ile Gly Glu Leu Ala Lys Lys Ala ArgAsn Asn 305 310 315 320 Lys Leu Thr Leu Ser Glu Leu Glu Gly Gly Ser PheThr Ile Thr Asn 325 330 335 Gly Gly Thr Phe Gly Ser Leu Met Ser Thr ProIle Leu Asn Ser Pro 340 345 350 Gln Val Gly Ile Leu Gly Met His Lys IleGln Leu Arg Pro Val Ala 355 360 365 Ile Asp Glu Glu Arg Phe Glu Asn ArgPro Met Met Tyr Ile Ala Leu 370 375 380 Ser Tyr Asp His Arg Ile Val AspGly Lys Glu Ala Val Gly Phe Leu 385 390 395 400 Val Thr Ile Lys Asn LeuLeu Glu Asp Pro Glu Gln Leu Leu Leu Glu 405 410 415 Gly 1254 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p4c52)“ CDS 1..1251 39 ATG GCG GAA ATT AAG GTA CCT GAA TTA GCA GAA TCAATC TCA GAA GGA 48 Met Ala Glu Ile Lys Val Pro Glu Leu Ala Glu Ser IleSer Glu Gly 1 5 10 15 ACA ATA GCC CAA TGG TTA AAG CAG CCT GGT GAC TATGTA GAA CAG GGT 96 Thr Ile Ala Gln Trp Leu Lys Gln Pro Gly Asp Tyr ValGlu Gln Gly 20 25 30 GAA TAT CTG CTT GAA CTA GAA ACG GAT AAA GTG AAT GTTGAA TTG ACA 144 Glu Tyr Leu Leu Glu Leu Glu Thr Asp Lys Val Asn Val GluLeu Thr 35 40 45 GCA GAA GAA TCG GGT GTA CTT CAA GAG GTA TTG AAA GAT TCGGGT GAT 192 Ala Glu Glu Ser Gly Val Leu Gln Glu Val Leu Lys Asp Ser GlyAsp 50 55 60 ACC GTC CAG GTC GGA GAA ATT ATC GGT ACG ATT TCA GAA GGC GCGGGT 240 Thr Val Gln Val Gly Glu Ile Ile Gly Thr Ile Ser Glu Gly Ala Gly65 70 75 80 GAA AGT TCT GCC CCT GCT CCT ACA GAG AAA ACA GAA AGC AAG GAAAGC 288 Glu Ser Ser Ala Pro Ala Pro Thr Glu Lys Thr Glu Ser Lys Glu Ser85 90 95 GTA AAA GAA GAG AAA CAG GCT GAA CCA GCT GCA CAA GAG GTG AGC GAG336 Val Lys Glu Glu Lys Gln Ala Glu Pro Ala Ala Gln Glu Val Ser Glu 100105 110 GAA GCA CAA TCT GAA GCA AAA TCA AGA ACG ATC GCT TCT CCG TCG GCC384 Glu Ala Gln Ser Glu Ala Lys Ser Arg Thr Ile Ala Ser Pro Ser Ala 115120 125 CGT AAG CTT GCG AGA GAA AAA GGA ATT GAC CTG TCT CAA GTT CCA ACT432 Arg Lys Leu Ala Arg Glu Lys Gly Ile Asp Leu Ser Gln Val Pro Thr 130135 140 GGA GAT CCG CTT GGA AGA GTG CGC AAG CAG GAT GTC GAA GCG TAC GAA480 Gly Asp Pro Leu Gly Arg Val Arg Lys Gln Asp Val Glu Ala Tyr Glu 145150 155 160 AAA CCG GCA TCA AAA CCG GCT CCT CAG CAA AAG CAG CAG CCT CAGGCT 528 Lys Pro Ala Ser Lys Pro Ala Pro Gln Gln Lys Gln Gln Pro Gln Ala165 170 175 CAA AAA GCA CAG CAA AGC TTT GAC AAA CCT GTT GAA GTG CAA AAAATG 576 Gln Lys Ala Gln Gln Ser Phe Asp Lys Pro Val Glu Val Gln Lys Met180 185 190 TCA CGC CGC AGA CAA ACG ATT GCA AAA CGC CTT GTA GAG GTA CAGCAA 624 Ser Arg Arg Arg Gln Thr Ile Ala Lys Arg Leu Val Glu Val Gln Gln195 200 205 ACA TCA GCG ATG CTG ACT ACA TTT AAT GAA GTG GAC ATG ACG GCTGTC 672 Thr Ser Ala Met Leu Thr Thr Phe Asn Glu Val Asp Met Thr Ala Val210 215 220 ATG AAT CTC AGA AAA CGC CGC AAA GAT CAA TTT TTT GAG CAA AATGAA 720 Met Asn Leu Arg Lys Arg Arg Lys Asp Gln Phe Phe Glu Gln Asn Glu225 230 235 240 GTG AAG CTC GGC TTT ATG TCT TTC TTC ACG AAA GCG GTC GTGGCT GCA 768 Val Lys Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val AlaAla 245 250 255 TTG AAA AAA TAT CCG CTG TTG AAT GCA GAA ATT CAA GGC GATGAG TTG 816 Leu Lys Lys Tyr Pro Leu Leu Asn Ala Glu Ile Gln Gly Asp GluLeu 260 265 270 ATC GTT AAA AAA TTC TAC GAT ATC GGA ATC GCT GTT GCT GCTGTA GAA 864 Ile Val Lys Lys Phe Tyr Asp Ile Gly Ile Ala Val Ala Ala ValGlu 275 280 285 GGT CTT GTC GTT CCG GTT GTA CGG GAT GCG GAT CGC CTG ACATTT GCA 912 Gly Leu Val Val Pro Val Val Arg Asp Ala Asp Arg Leu Thr PheAla 290 295 300 GGA ATC GAA AAA GAG ATC GGC GAG CTT GCG AAA AAA GCA AGAAAC AAT 960 Gly Ile Glu Lys Glu Ile Gly Glu Leu Ala Lys Lys Ala Arg AsnAsn 305 310 315 320 AAA TTA ACC CTT AGC GAG CTT GAG GGA GGC TCC TTC ACGATT ACA AAC 1008 Lys Leu Thr Leu Ser Glu Leu Glu Gly Gly Ser Phe Thr IleThr Asn 325 330 335 GGA GGG ACT TTC GGT TCA TTG ATG TCA ACT CCA ATT TTAAAC AGC CCG 1056 Gly Gly Thr Phe Gly Ser Leu Met Ser Thr Pro Ile Leu AsnSer Pro 340 345 350 CAA GTC GGT ATA CTG GGC ATG CAT AAG ATT CAG CTG CGCCCT GTA GCC 1104 Gln Val Gly Ile Leu Gly Met His Lys Ile Gln Leu Arg ProVal Ala 355 360 365 ATT GAT GAA GAG CGT TTC GAA AAC CGT CCG ATG ATG TATATC GCT TTA 1152 Ile Asp Glu Glu Arg Phe Glu Asn Arg Pro Met Met Tyr IleAla Leu 370 375 380 TCT TAT GAT CAC CGA ATT GTA GAC GGT AAA GAA GCG GTTGGT TTC CTC 1200 Ser Tyr Asp His Arg Ile Val Asp Gly Lys Glu Ala Val GlyPhe Leu 385 390 395 400 GTG ACA ATC AAA AAT TTA CTG GAA GAT CCT GAA CAGCTT TTA TTA GAA 1248 Val Thr Ile Lys Asn Leu Leu Glu Asp Pro Glu Gln LeuLeu Leu Glu 405 410 415 GGA TAA 1254 Gly 417 amino acids amino acidlinear protein 40 Met Ala Glu Ile Lys Val Pro Glu Leu Ala Glu Ser IleSer Glu Gly 1 5 10 15 Thr Ile Ala Gln Trp Leu Lys Gln Pro Gly Asp TyrVal Glu Gln Gly 20 25 30 Glu Tyr Leu Leu Glu Leu Glu Thr Asp Lys Val AsnVal Glu Leu Thr 35 40 45 Ala Glu Glu Ser Gly Val Leu Gln Glu Val Leu LysAsp Ser Gly Asp 50 55 60 Thr Val Gln Val Gly Glu Ile Ile Gly Thr Ile SerGlu Gly Ala Gly 65 70 75 80 Glu Ser Ser Ala Pro Ala Pro Thr Glu Lys ThrGlu Ser Lys Glu Ser 85 90 95 Val Lys Glu Glu Lys Gln Ala Glu Pro Ala AlaGln Glu Val Ser Glu 100 105 110 Glu Ala Gln Ser Glu Ala Lys Ser Arg ThrIle Ala Ser Pro Ser Ala 115 120 125 Arg Lys Leu Ala Arg Glu Lys Gly IleAsp Leu Ser Gln Val Pro Thr 130 135 140 Gly Asp Pro Leu Gly Arg Val ArgLys Gln Asp Val Glu Ala Tyr Glu 145 150 155 160 Lys Pro Ala Ser Lys ProAla Pro Gln Gln Lys Gln Gln Pro Gln Ala 165 170 175 Gln Lys Ala Gln GlnSer Phe Asp Lys Pro Val Glu Val Gln Lys Met 180 185 190 Ser Arg Arg ArgGln Thr Ile Ala Lys Arg Leu Val Glu Val Gln Gln 195 200 205 Thr Ser AlaMet Leu Thr Thr Phe Asn Glu Val Asp Met Thr Ala Val 210 215 220 Met AsnLeu Arg Lys Arg Arg Lys Asp Gln Phe Phe Glu Gln Asn Glu 225 230 235 240Val Lys Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val Ala Ala 245 250255 Leu Lys Lys Tyr Pro Leu Leu Asn Ala Glu Ile Gln Gly Asp Glu Leu 260265 270 Ile Val Lys Lys Phe Tyr Asp Ile Gly Ile Ala Val Ala Ala Val Glu275 280 285 Gly Leu Val Val Pro Val Val Arg Asp Ala Asp Arg Leu Thr PheAla 290 295 300 Gly Ile Glu Lys Glu Ile Gly Glu Leu Ala Lys Lys Ala ArgAsn Asn 305 310 315 320 Lys Leu Thr Leu Ser Glu Leu Glu Gly Gly Ser PheThr Ile Thr Asn 325 330 335 Gly Gly Thr Phe Gly Ser Leu Met Ser Thr ProIle Leu Asn Ser Pro 340 345 350 Gln Val Gly Ile Leu Gly Met His Lys IleGln Leu Arg Pro Val Ala 355 360 365 Ile Asp Glu Glu Arg Phe Glu Asn ArgPro Met Met Tyr Ile Ala Leu 370 375 380 Ser Tyr Asp His Arg Ile Val AspGly Lys Glu Ala Val Gly Phe Leu 385 390 395 400 Val Thr Ile Lys Asn LeuLeu Glu Asp Pro Glu Gln Leu Leu Leu Glu 405 410 415 Gly 1254 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p10b2)“ CDS 1..1251 41 ATG GCG GAA ATT AAG GTA CCT GAA TTA GCA GAA TCAATC TCA GAA GGA 48 Met Ala Glu Ile Lys Val Pro Glu Leu Ala Glu Ser IleSer Glu Gly 1 5 10 15 ACA ATA GCC CAA TGG TTA AAG CAG CCT GGT GAC TATGTA GAA CAG GGT 96 Thr Ile Ala Gln Trp Leu Lys Gln Pro Gly Asp Tyr ValGlu Gln Gly 20 25 30 GAA TAT CTG CTT GAA CTA GAA ACG GAT AAA GTG AAT GTTGAA TTG ACA 144 Glu Tyr Leu Leu Glu Leu Glu Thr Asp Lys Val Asn Val GluLeu Thr 35 40 45 GCA GAA GAA TCG GGT GTA CTT CAA GAG GTA TTG AAA GAT TCGGGT GAT 192 Ala Glu Glu Ser Gly Val Leu Gln Glu Val Leu Lys Asp Ser GlyAsp 50 55 60 ACC GTC CAG GTC GGA GAA ATT ATC GGT ACG ATT TCA GAA GGC GCGGGT 240 Thr Val Gln Val Gly Glu Ile Ile Gly Thr Ile Ser Glu Gly Ala Gly65 70 75 80 GAA AGT TCT GCC CCT GCT CCT ACA GAG AAA ACA GAA AGC AAG GAAAGC 288 Glu Ser Ser Ala Pro Ala Pro Thr Glu Lys Thr Glu Ser Lys Glu Ser85 90 95 GTA AAA GAA GAG AAA CAG GCT GAA CCA GCT GCA CAA GAG GTG AGC GAG336 Val Lys Glu Glu Lys Gln Ala Glu Pro Ala Ala Gln Glu Val Ser Glu 100105 110 GAA GCA CAA TCT GAA GCA AAA TCA AGA ACG ATC GCT TCT CCG TCG GCC384 Glu Ala Gln Ser Glu Ala Lys Ser Arg Thr Ile Ala Ser Pro Ser Ala 115120 125 CGT AAG CTT GCG AGA GAA AAA GGA ATT GAC CTG TCT CAA GTT CCA ACT432 Arg Lys Leu Ala Arg Glu Lys Gly Ile Asp Leu Ser Gln Val Pro Thr 130135 140 GGA GAT CCG CTT GGA AGA GTG CGC AAG CAG GAT GTC GAA GCG TAC GAA480 Gly Asp Pro Leu Gly Arg Val Arg Lys Gln Asp Val Glu Ala Tyr Glu 145150 155 160 AAA CCG GCA TCA AAA CCG GCT CCT CAG CAA AAG CAG CAG CCT CAGGCT 528 Lys Pro Ala Ser Lys Pro Ala Pro Gln Gln Lys Gln Gln Pro Gln Ala165 170 175 CAA AAA GCA CAG CAA AGC TTT GAC AAA CCT GTT GAA GTG CAA AAAATG 576 Gln Lys Ala Gln Gln Ser Phe Asp Lys Pro Val Glu Val Gln Lys Met180 185 190 TCA CGC CGC AGA CAA ACG ATT GCA AAA CGC CTT GTA GAG GTA CAGCAA 624 Ser Arg Arg Arg Gln Thr Ile Ala Lys Arg Leu Val Glu Val Gln Gln195 200 205 ACA TCA GCG ATG CTG ACT ACA TTT AAT GAA GTG GAC ATG ACG GCTGTC 672 Thr Ser Ala Met Leu Thr Thr Phe Asn Glu Val Asp Met Thr Ala Val210 215 220 ATG AAT CTC AGA AAA CGC CGC AAA GAT CAA TTT TTT GAG CAA AATGAA 720 Met Asn Leu Arg Lys Arg Arg Lys Asp Gln Phe Phe Glu Gln Asn Glu225 230 235 240 GTG AAG CTC GGC TTT ATG TCT TTC TTC ACG AAA GCG GTC GTGGCT GCA 768 Val Lys Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val AlaAla 245 250 255 TTG AAA AAA TAT CCG CTG TTG AAT GCA GAA ATT CAA GGC GATGAG TTG 816 Leu Lys Lys Tyr Pro Leu Leu Asn Ala Glu Ile Gln Gly Asp GluLeu 260 265 270 ATC GTT AAA AAA TTC TAC GAT ATC GGA ATC GCT GTT GCT GCTGTA GAA 864 Ile Val Lys Lys Phe Tyr Asp Ile Gly Ile Ala Val Ala Ala ValGlu 275 280 285 GGT CTT GTC GTT CCG GTT GTA CGG GAT GCG GAT CGC CTG ACATTT GCA 912 Gly Leu Val Val Pro Val Val Arg Asp Ala Asp Arg Leu Thr PheAla 290 295 300 GGA ATC GAA AAA GAG ATC GGC GAG CTT GCG AAA AAA GCA AGAAAC AAT 960 Gly Ile Glu Lys Glu Ile Gly Glu Leu Ala Lys Lys Ala Arg AsnAsn 305 310 315 320 AAA TTA ACC CTT AGC GAG CTT GAG GGA GGC TCC TTC ACGATT ACA AAC 1008 Lys Leu Thr Leu Ser Glu Leu Glu Gly Gly Ser Phe Thr IleThr Asn 325 330 335 GGA GGG ACT TTC GGT TCA TTG ATG TCA ACT CCA ATT TTAAAC AGC CCG 1056 Gly Gly Thr Phe Gly Ser Leu Met Ser Thr Pro Ile Leu AsnSer Pro 340 345 350 CAA GTC GGT ATA CTG GGC ATG CAT AAG ATT CAG CTG CGCCCT GTA GCC 1104 Gln Val Gly Ile Leu Gly Met His Lys Ile Gln Leu Arg ProVal Ala 355 360 365 ATT GAT GAA GAG CGT TTC GAA AAC CGT CCG ATG ATG TATATC GCT TTA 1152 Ile Asp Glu Glu Arg Phe Glu Asn Arg Pro Met Met Tyr IleAla Leu 370 375 380 TCT TAT GAT CAC CGA ATT GTA GAC GGT AAA GAA GCG GTTGGT TTC CTC 1200 Ser Tyr Asp His Arg Ile Val Asp Gly Lys Glu Ala Val GlyPhe Leu 385 390 395 400 GTG ACA ATC AAA AAT TTA CTG GAA GAT CCT GAA CAGCTT TTA TTA GAA 1248 Val Thr Ile Lys Asn Leu Leu Glu Asp Pro Glu Gln LeuLeu Leu Glu 405 410 415 GGA TAA 1254 Gly 417 amino acids amino acidlinear protein 42 Met Ala Glu Ile Lys Val Pro Glu Leu Ala Glu Ser IleSer Glu Gly 1 5 10 15 Thr Ile Ala Gln Trp Leu Lys Gln Pro Gly Asp TyrVal Glu Gln Gly 20 25 30 Glu Tyr Leu Leu Glu Leu Glu Thr Asp Lys Val AsnVal Glu Leu Thr 35 40 45 Ala Glu Glu Ser Gly Val Leu Gln Glu Val Leu LysAsp Ser Gly Asp 50 55 60 Thr Val Gln Val Gly Glu Ile Ile Gly Thr Ile SerGlu Gly Ala Gly 65 70 75 80 Glu Ser Ser Ala Pro Ala Pro Thr Glu Lys ThrGlu Ser Lys Glu Ser 85 90 95 Val Lys Glu Glu Lys Gln Ala Glu Pro Ala AlaGln Glu Val Ser Glu 100 105 110 Glu Ala Gln Ser Glu Ala Lys Ser Arg ThrIle Ala Ser Pro Ser Ala 115 120 125 Arg Lys Leu Ala Arg Glu Lys Gly IleAsp Leu Ser Gln Val Pro Thr 130 135 140 Gly Asp Pro Leu Gly Arg Val ArgLys Gln Asp Val Glu Ala Tyr Glu 145 150 155 160 Lys Pro Ala Ser Lys ProAla Pro Gln Gln Lys Gln Gln Pro Gln Ala 165 170 175 Gln Lys Ala Gln GlnSer Phe Asp Lys Pro Val Glu Val Gln Lys Met 180 185 190 Ser Arg Arg ArgGln Thr Ile Ala Lys Arg Leu Val Glu Val Gln Gln 195 200 205 Thr Ser AlaMet Leu Thr Thr Phe Asn Glu Val Asp Met Thr Ala Val 210 215 220 Met AsnLeu Arg Lys Arg Arg Lys Asp Gln Phe Phe Glu Gln Asn Glu 225 230 235 240Val Lys Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val Ala Ala 245 250255 Leu Lys Lys Tyr Pro Leu Leu Asn Ala Glu Ile Gln Gly Asp Glu Leu 260265 270 Ile Val Lys Lys Phe Tyr Asp Ile Gly Ile Ala Val Ala Ala Val Glu275 280 285 Gly Leu Val Val Pro Val Val Arg Asp Ala Asp Arg Leu Thr PheAla 290 295 300 Gly Ile Glu Lys Glu Ile Gly Glu Leu Ala Lys Lys Ala ArgAsn Asn 305 310 315 320 Lys Leu Thr Leu Ser Glu Leu Glu Gly Gly Ser PheThr Ile Thr Asn 325 330 335 Gly Gly Thr Phe Gly Ser Leu Met Ser Thr ProIle Leu Asn Ser Pro 340 345 350 Gln Val Gly Ile Leu Gly Met His Lys IleGln Leu Arg Pro Val Ala 355 360 365 Ile Asp Glu Glu Arg Phe Glu Asn ArgPro Met Met Tyr Ile Ala Leu 370 375 380 Ser Tyr Asp His Arg Ile Val AspGly Lys Glu Ala Val Gly Phe Leu 385 390 395 400 Val Thr Ile Lys Asn LeuLeu Glu Asp Pro Glu Gln Leu Leu Leu Glu 405 410 415 Gly 524 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p10c20)“ 43 TTTCGAAGAT CGGCTCTTAC AATATTGACG GTAAAGAACA GTTGGATTCTTAAAAACAAT 60 TAAAGAATTA ATTGAAAACC CAAAGACTTA TTATAGAATC TTAATCCACAACACAAAATA 120 GTTTACTATT CCTAAAAGCG GGATTAAATC AATAACAAAC AGCATAAGATTATTTCCTAG 180 TCGAAATATC TTACTGCTGT ACTTTATTTT TATAATGATC TTTAATGTGGGTTTAATTTT 240 GACTACTTAA AAATATATCA TTTCTATTGA AATAGACTCA CAATACAAATATAGTAGAAT 300 GCGTGTTTCA ATATGCTAAT GAATGCATTT TAGATATAAC AAACGAGAAATATATGAATT 360 CTATAAGCGC CTTTAGTTAA TTTAAATCTC TGAACATGAT GTAATTCGCTTTATGGACAC 420 CACATTATAT GTTTTCATGT CCTACAACAC AACACATATT CAATTGTATATACAGATATT 480 CTTAATGACA CACTCATAGC CCCATAACAA TATATGTTAC CTAT 524 2799base pairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p12c32)“ CDS 1..2796 44 ATG ACT AAC GAA AGA AAA GAA GTT TCAGAG GCT CCT GTA AAC TTC GGT 48 Met Thr Asn Glu Arg Lys Glu Val Ser GluAla Pro Val Asn Phe Gly 1 5 10 15 GCG AAT TTA GGT CTA ATG TTA GAT CTATAT GAT GAC TTT TTA CAA GAT 96 Ala Asn Leu Gly Leu Met Leu Asp Leu TyrAsp Asp Phe Leu Gln Asp 20 25 30 CCA TCA TCT GTA CCA GAA GAT TTA CAA GTCTTA TTC AGC ACA ATT AAG 144 Pro Ser Ser Val Pro Glu Asp Leu Gln Val LeuPhe Ser Thr Ile Lys 35 40 45 AAT GAT GAC TCA ATT GTA CCA GCT TTA AAA AGTACA AGT AGT CAA AAT 192 Asn Asp Asp Ser Ile Val Pro Ala Leu Lys Ser ThrSer Ser Gln Asn 50 55 60 AGC GAC GGC ACA ATT AAG CGT GTC ATG CGT TTA ATTGAT AAT ATT CGC 240 Ser Asp Gly Thr Ile Lys Arg Val Met Arg Leu Ile AspAsn Ile Arg 65 70 75 80 CAA TAC GGG CAT CTT AAA GCC GAT ATT TAT CCT GTAAAT CCT CCA AAA 288 Gln Tyr Gly His Leu Lys Ala Asp Ile Tyr Pro Val AsnPro Pro Lys 85 90 95 AGG AAA CAT GTA CCT AAA TTA GAG ATT GAA GAC TTT GATTTA GAT CAA 336 Arg Lys His Val Pro Lys Leu Glu Ile Glu Asp Phe Asp LeuAsp Gln 100 105 110 CAG ACT TTG GAA GGT ATA TCA GCA GGA ATT GTT TCA GATCAC TTT GCC 384 Gln Thr Leu Glu Gly Ile Ser Ala Gly Ile Val Ser Asp HisPhe Ala 115 120 125 GAC ATT TAT GAT AAT GCT TAT GAA GCA ATT TTA AGA ATGGAA AAA CGT 432 Asp Ile Tyr Asp Asn Ala Tyr Glu Ala Ile Leu Arg Met GluLys Arg 130 135 140 TAC AAA GGA CCA ATT GCA TTT GAG TAT ACA CAT ATT AATAAC AAT ACC 480 Tyr Lys Gly Pro Ile Ala Phe Glu Tyr Thr His Ile Asn AsnAsn Thr 145 150 155 160 GAA CGT GGT TGG TTA AAA AGA AGA ATT GAA ACG CCATAT AAA GTA ACG 528 Glu Arg Gly Trp Leu Lys Arg Arg Ile Glu Thr Pro TyrLys Val Thr 165 170 175 TTA AAT AAT AAC GAA AAA AGG GCA CTA TTC AAA CAATTA GCG TAT GTT 576 Leu Asn Asn Asn Glu Lys Arg Ala Leu Phe Lys Gln LeuAla Tyr Val 180 185 190 GAA GGG TTT GAA AAA TAT CTT CAT AAA AAC TTC GTTGGT GCA AAG CGT 624 Glu Gly Phe Glu Lys Tyr Leu His Lys Asn Phe Val GlyAla Lys Arg 195 200 205 TTT TCA ATT GAA GGG GTA GAC GCA CTT GTA CCG ATGTTA CAA CGT ACT 672 Phe Ser Ile Glu Gly Val Asp Ala Leu Val Pro Met LeuGln Arg Thr 210 215 220 ATT ACG ATT GCT GCG AAA GAA GGT ATT AAA AAT ATACAA ATA GGC ATG 720 Ile Thr Ile Ala Ala Lys Glu Gly Ile Lys Asn Ile GlnIle Gly Met 225 230 235 240 GCT CAC CGT GGA CGT TTA AAC GTT TTA ACG CATGTC TTA GAA AAA CCG 768 Ala His Arg Gly Arg Leu Asn Val Leu Thr His ValLeu Glu Lys Pro 245 250 255 TAC GAA ATG ATG ATT TCA GAA TTT ATG CAT ACAGAT CCA ATG AAA TTC 816 Tyr Glu Met Met Ile Ser Glu Phe Met His Thr AspPro Met Lys Phe 260 265 270 TTA CCT GAA GAT GGT AGC TTG CAG TTA ACT GCTGGA TGG ACT GGT GAT 864 Leu Pro Glu Asp Gly Ser Leu Gln Leu Thr Ala GlyTrp Thr Gly Asp 275 280 285 GTG AAA TAT CAC CTT GGT GGC ATT AAA ACT ACTGAT TCA TAC GGT ACA 912 Val Lys Tyr His Leu Gly Gly Ile Lys Thr Thr AspSer Tyr Gly Thr 290 295 300 ATG CAG CGT ATT GCA CTG GCT AAC AAT CCA AGTCAC TTG GAA ATT GTT 960 Met Gln Arg Ile Ala Leu Ala Asn Asn Pro Ser HisLeu Glu Ile Val 305 310 315 320 GCA CCT GTT GTT GAG GGG CGT ACG AGA GCAGCA CAA GAT GAT ACA CAA 1008 Ala Pro Val Val Glu Gly Arg Thr Arg Ala AlaGln Asp Asp Thr Gln 325 330 335 CGA GCT GGG GCT CCG ACG ACT GAT CAT CATAAA GCA ATG CCA ATT ATT 1056 Arg Ala Gly Ala Pro Thr Thr Asp His His LysAla Met Pro Ile Ile 340 345 350 ATA CAT GGC GAT GCT GCT TAT CCT GGT CAAGGA ATT AAC TTC GAA ACA 1104 Ile His Gly Asp Ala Ala Tyr Pro Gly Gln GlyIle Asn Phe Glu Thr 355 360 365 ATG AAC TTA GGA AAC TTG AAA GGC TAT TCTACG GGT GGT TCA TTG CAT 1152 Met Asn Leu Gly Asn Leu Lys Gly Tyr Ser ThrGly Gly Ser Leu His 370 375 380 ATT ATT ACT AAC AAT AGA ATT GGA TTT ACTACA GAA CCA ATT GAT GCA 1200 Ile Ile Thr Asn Asn Arg Ile Gly Phe Thr ThrGlu Pro Ile Asp Ala 385 390 395 400 CGT TCA ACA ACT TAT TCT ACA GAT GTGGCC AAA GGT TAT GAT GTG CCA 1248 Arg Ser Thr Thr Tyr Ser Thr Asp Val AlaLys Gly Tyr Asp Val Pro 405 410 415 ATA TTC CAT GTC AAT GCA GAT GAC GTTGAA GCT ACT ATT GAA GCA ATT 1296 Ile Phe His Val Asn Ala Asp Asp Val GluAla Thr Ile Glu Ala Ile 420 425 430 GAT ATT GCA ATG GAA TTT AGA AAA GAGTTT CAT AAA GAC GTC GTT ATT 1344 Asp Ile Ala Met Glu Phe Arg Lys Glu PheHis Lys Asp Val Val Ile 435 440 445 GAT TTA GTA GGT TAT CGT CGT TTC GGACAT AAC GAA ATG GAT GAA CCA 1392 Asp Leu Val Gly Tyr Arg Arg Phe Gly HisAsn Glu Met Asp Glu Pro 450 455 460 TCA ATT ACT AAT CCA GTT CCT TAT CAGAAT ATT CGC AAA CAT GAC TCT 1440 Ser Ile Thr Asn Pro Val Pro Tyr Gln AsnIle Arg Lys His Asp Ser 465 470 475 480 GTT GAA TAT GTG TTT GGT AAA AAGCTT GTT AAT GAA GGT GTC ATT TCA 1488 Val Glu Tyr Val Phe Gly Lys Lys LeuVal Asn Glu Gly Val Ile Ser 485 490 495 GAA GAT GAA ATG CAT TCA TTT ATAGAA CAA GTC CAA AAG GAA CTA AGA 1536 Glu Asp Glu Met His Ser Phe Ile GluGln Val Gln Lys Glu Leu Arg 500 505 510 CAA GCT CAT GAT AAA ATT AAT AAAGCT GAT AAA ATG GAT AAT CCA GAT 1584 Gln Ala His Asp Lys Ile Asn Lys AlaAsp Lys Met Asp Asn Pro Asp 515 520 525 ATG GAA AAG CCT GCA GAT CTT GCATTA CCG TTA CAA GCA GAC GAA CAA 1632 Met Glu Lys Pro Ala Asp Leu Ala LeuPro Leu Gln Ala Asp Glu Gln 530 535 540 TCA TTT ACT TTT GAT CAC TTG AAAGAA ATA AAT GAT GCA TTG TTA ACA 1680 Ser Phe Thr Phe Asp His Leu Lys GluIle Asn Asp Ala Leu Leu Thr 545 550 555 560 TAT CCG GAT GGC TTT AAC ATTTTG AAA AAG TTA AAC AAA GTT CTT GAG 1728 Tyr Pro Asp Gly Phe Asn Ile LeuLys Lys Leu Asn Lys Val Leu Glu 565 570 575 AAG CGT CAT GAG CCG TTT AATAAA GAA GAT GGT TTA GTT GAT TGG GCA 1776 Lys Arg His Glu Pro Phe Asn LysGlu Asp Gly Leu Val Asp Trp Ala 580 585 590 CAA GCA GAA CAA CTT GCA TTTGCG ACA ATT TTA CAA GAT GGT ACA CCG 1824 Gln Ala Glu Gln Leu Ala Phe AlaThr Ile Leu Gln Asp Gly Thr Pro 595 600 605 ATT CGC TTA ACT GGT CAA GATAGT GAA CGT GGT ACA TTC AGT CAT AGG 1872 Ile Arg Leu Thr Gly Gln Asp SerGlu Arg Gly Thr Phe Ser His Arg 610 615 620 CAT GCC GTG TTA CAT GAT GAGCAA ACA GGT GAA ACA TAT ACA CCT TTA 1920 His Ala Val Leu His Asp Glu GlnThr Gly Glu Thr Tyr Thr Pro Leu 625 630 635 640 CAT CAT GTT CCT GAT CAAAAA GCG ACA TTT GAT ATA CAC AAT TCT CCG 1968 His His Val Pro Asp Gln LysAla Thr Phe Asp Ile His Asn Ser Pro 645 650 655 CTT TCA GAA GCA GCA GTAGTT GGT TTT GAA TAC GGC TAT AAT GTG GAA 2016 Leu Ser Glu Ala Ala Val ValGly Phe Glu Tyr Gly Tyr Asn Val Glu 660 665 670 AAC AAA AAA AGC TTC AATATT TGG GAA GCA CAA TAT GGT GAT TTT GCA 2064 Asn Lys Lys Ser Phe Asn IleTrp Glu Ala Gln Tyr Gly Asp Phe Ala 675 680 685 AAT ATG TCA CAA ATG ATTTTT GAC AAC TTC TTA TTC AGT TCT CGC TCA 2112 Asn Met Ser Gln Met Ile PheAsp Asn Phe Leu Phe Ser Ser Arg Ser 690 695 700 AAA TGG GGA GAA CGT TCAGGA TTA ACA TTA TTC TTA CCT CAT GCA TAT 2160 Lys Trp Gly Glu Arg Ser GlyLeu Thr Leu Phe Leu Pro His Ala Tyr 705 710 715 720 GAG GGT CAA GGG CCTGAA CAT TCA TCA GCA AGA TTA GAG CGA TTT TTA 2208 Glu Gly Gln Gly Pro GluHis Ser Ser Ala Arg Leu Glu Arg Phe Leu 725 730 735 CAA TTA GCT GCT GAAAAT AAT TGC ACA GTT GTC AAC TTA TCT AGT TCA 2256 Gln Leu Ala Ala Glu AsnAsn Cys Thr Val Val Asn Leu Ser Ser Ser 740 745 750 AGT AAT TAT TTC CACTTA TTG CGT GCA CAA GCG GCT AGT TTA GAT TCT 2304 Ser Asn Tyr Phe His LeuLeu Arg Ala Gln Ala Ala Ser Leu Asp Ser 755 760 765 GAA CAA ATG CGA CCATTG GTT GTT ATG TCA CCA AAA AGC TTA CTG AGA 2352 Glu Gln Met Arg Pro LeuVal Val Met Ser Pro Lys Ser Leu Leu Arg 770 775 780 AAT AAA ACA GTT GCAAAA CCA ATT GAT GAA TTT ACT TCT GGT GGA TTT 2400 Asn Lys Thr Val Ala LysPro Ile Asp Glu Phe Thr Ser Gly Gly Phe 785 790 795 800 GAG CCA ATT TTGACA GAA TCA TAT CAA GCG GAT AAG GTT ACA AAA GTT 2448 Glu Pro Ile Leu ThrGlu Ser Tyr Gln Ala Asp Lys Val Thr Lys Val 805 810 815 ATT TTG GCA ACTGGT AAA ATG TTC ATT GAT TTA AAA GAA GCA TTA GCT 2496 Ile Leu Ala Thr GlyLys Met Phe Ile Asp Leu Lys Glu Ala Leu Ala 820 825 830 AAA AAT CCA GACGAA TCA GTA TTA CTC GTT GCG ATT GAA AGA TTG TAT 2544 Lys Asn Pro Asp GluSer Val Leu Leu Val Ala Ile Glu Arg Leu Tyr 835 840 845 CCA TTC CCA GAGGAA GAG ATT GAA GCA TTA CTA GCA CAA TTG CCA AAC 2592 Pro Phe Pro Glu GluGlu Ile Glu Ala Leu Leu Ala Gln Leu Pro Asn 850 855 860 CTT GAA GAA GTGTCA TGG GTA CAA GAA GAA CCT AAA AAT CAA GGT GCA 2640 Leu Glu Glu Val SerTrp Val Gln Glu Glu Pro Lys Asn Gln Gly Ala 865 870 875 880 TGG TTA TATGTC TAT CCA TAT GTT AAA GTG CTA GTT GCA GAT AAA TAT 2688 Trp Leu Tyr ValTyr Pro Tyr Val Lys Val Leu Val Ala Asp Lys Tyr 885 890 895 GAT TTA AGTTAT CAT GGC AGA ATT CAA AGG GCT GCT CCA GCT GAA GGC 2736 Asp Leu Ser TyrHis Gly Arg Ile Gln Arg Ala Ala Pro Ala Glu Gly 900 905 910 GAT GGA GAAATT CAT AAA CTT GTT CAA AAT AAA ATT ATA GAA AAT GCA 2784 Asp Gly Glu IleHis Lys Leu Val Gln Asn Lys Ile Ile Glu Asn Ala 915 920 925 TTA AAA AATAAC TAG 2799 Leu Lys Asn Asn 930 932 amino acids amino acid linearprotein 45 Met Thr Asn Glu Arg Lys Glu Val Ser Glu Ala Pro Val Asn PheGly 1 5 10 15 Ala Asn Leu Gly Leu Met Leu Asp Leu Tyr Asp Asp Phe LeuGln Asp 20 25 30 Pro Ser Ser Val Pro Glu Asp Leu Gln Val Leu Phe Ser ThrIle Lys 35 40 45 Asn Asp Asp Ser Ile Val Pro Ala Leu Lys Ser Thr Ser SerGln Asn 50 55 60 Ser Asp Gly Thr Ile Lys Arg Val Met Arg Leu Ile Asp AsnIle Arg 65 70 75 80 Gln Tyr Gly His Leu Lys Ala Asp Ile Tyr Pro Val AsnPro Pro Lys 85 90 95 Arg Lys His Val Pro Lys Leu Glu Ile Glu Asp Phe AspLeu Asp Gln 100 105 110 Gln Thr Leu Glu Gly Ile Ser Ala Gly Ile Val SerAsp His Phe Ala 115 120 125 Asp Ile Tyr Asp Asn Ala Tyr Glu Ala Ile LeuArg Met Glu Lys Arg 130 135 140 Tyr Lys Gly Pro Ile Ala Phe Glu Tyr ThrHis Ile Asn Asn Asn Thr 145 150 155 160 Glu Arg Gly Trp Leu Lys Arg ArgIle Glu Thr Pro Tyr Lys Val Thr 165 170 175 Leu Asn Asn Asn Glu Lys ArgAla Leu Phe Lys Gln Leu Ala Tyr Val 180 185 190 Glu Gly Phe Glu Lys TyrLeu His Lys Asn Phe Val Gly Ala Lys Arg 195 200 205 Phe Ser Ile Glu GlyVal Asp Ala Leu Val Pro Met Leu Gln Arg Thr 210 215 220 Ile Thr Ile AlaAla Lys Glu Gly Ile Lys Asn Ile Gln Ile Gly Met 225 230 235 240 Ala HisArg Gly Arg Leu Asn Val Leu Thr His Val Leu Glu Lys Pro 245 250 255 TyrGlu Met Met Ile Ser Glu Phe Met His Thr Asp Pro Met Lys Phe 260 265 270Leu Pro Glu Asp Gly Ser Leu Gln Leu Thr Ala Gly Trp Thr Gly Asp 275 280285 Val Lys Tyr His Leu Gly Gly Ile Lys Thr Thr Asp Ser Tyr Gly Thr 290295 300 Met Gln Arg Ile Ala Leu Ala Asn Asn Pro Ser His Leu Glu Ile Val305 310 315 320 Ala Pro Val Val Glu Gly Arg Thr Arg Ala Ala Gln Asp AspThr Gln 325 330 335 Arg Ala Gly Ala Pro Thr Thr Asp His His Lys Ala MetPro Ile Ile 340 345 350 Ile His Gly Asp Ala Ala Tyr Pro Gly Gln Gly IleAsn Phe Glu Thr 355 360 365 Met Asn Leu Gly Asn Leu Lys Gly Tyr Ser ThrGly Gly Ser Leu His 370 375 380 Ile Ile Thr Asn Asn Arg Ile Gly Phe ThrThr Glu Pro Ile Asp Ala 385 390 395 400 Arg Ser Thr Thr Tyr Ser Thr AspVal Ala Lys Gly Tyr Asp Val Pro 405 410 415 Ile Phe His Val Asn Ala AspAsp Val Glu Ala Thr Ile Glu Ala Ile 420 425 430 Asp Ile Ala Met Glu PheArg Lys Glu Phe His Lys Asp Val Val Ile 435 440 445 Asp Leu Val Gly TyrArg Arg Phe Gly His Asn Glu Met Asp Glu Pro 450 455 460 Ser Ile Thr AsnPro Val Pro Tyr Gln Asn Ile Arg Lys His Asp Ser 465 470 475 480 Val GluTyr Val Phe Gly Lys Lys Leu Val Asn Glu Gly Val Ile Ser 485 490 495 GluAsp Glu Met His Ser Phe Ile Glu Gln Val Gln Lys Glu Leu Arg 500 505 510Gln Ala His Asp Lys Ile Asn Lys Ala Asp Lys Met Asp Asn Pro Asp 515 520525 Met Glu Lys Pro Ala Asp Leu Ala Leu Pro Leu Gln Ala Asp Glu Gln 530535 540 Ser Phe Thr Phe Asp His Leu Lys Glu Ile Asn Asp Ala Leu Leu Thr545 550 555 560 Tyr Pro Asp Gly Phe Asn Ile Leu Lys Lys Leu Asn Lys ValLeu Glu 565 570 575 Lys Arg His Glu Pro Phe Asn Lys Glu Asp Gly Leu ValAsp Trp Ala 580 585 590 Gln Ala Glu Gln Leu Ala Phe Ala Thr Ile Leu GlnAsp Gly Thr Pro 595 600 605 Ile Arg Leu Thr Gly Gln Asp Ser Glu Arg GlyThr Phe Ser His Arg 610 615 620 His Ala Val Leu His Asp Glu Gln Thr GlyGlu Thr Tyr Thr Pro Leu 625 630 635 640 His His Val Pro Asp Gln Lys AlaThr Phe Asp Ile His Asn Ser Pro 645 650 655 Leu Ser Glu Ala Ala Val ValGly Phe Glu Tyr Gly Tyr Asn Val Glu 660 665 670 Asn Lys Lys Ser Phe AsnIle Trp Glu Ala Gln Tyr Gly Asp Phe Ala 675 680 685 Asn Met Ser Gln MetIle Phe Asp Asn Phe Leu Phe Ser Ser Arg Ser 690 695 700 Lys Trp Gly GluArg Ser Gly Leu Thr Leu Phe Leu Pro His Ala Tyr 705 710 715 720 Glu GlyGln Gly Pro Glu His Ser Ser Ala Arg Leu Glu Arg Phe Leu 725 730 735 GlnLeu Ala Ala Glu Asn Asn Cys Thr Val Val Asn Leu Ser Ser Ser 740 745 750Ser Asn Tyr Phe His Leu Leu Arg Ala Gln Ala Ala Ser Leu Asp Ser 755 760765 Glu Gln Met Arg Pro Leu Val Val Met Ser Pro Lys Ser Leu Leu Arg 770775 780 Asn Lys Thr Val Ala Lys Pro Ile Asp Glu Phe Thr Ser Gly Gly Phe785 790 795 800 Glu Pro Ile Leu Thr Glu Ser Tyr Gln Ala Asp Lys Val ThrLys Val 805 810 815 Ile Leu Ala Thr Gly Lys Met Phe Ile Asp Leu Lys GluAla Leu Ala 820 825 830 Lys Asn Pro Asp Glu Ser Val Leu Leu Val Ala IleGlu Arg Leu Tyr 835 840 845 Pro Phe Pro Glu Glu Glu Ile Glu Ala Leu LeuAla Gln Leu Pro Asn 850 855 860 Leu Glu Glu Val Ser Trp Val Gln Glu GluPro Lys Asn Gln Gly Ala 865 870 875 880 Trp Leu Tyr Val Tyr Pro Tyr ValLys Val Leu Val Ala Asp Lys Tyr 885 890 895 Asp Leu Ser Tyr His Gly ArgIle Gln Arg Ala Ala Pro Ala Glu Gly 900 905 910 Asp Gly Glu Ile His LysLeu Val Gln Asn Lys Ile Ile Glu Asn Ala 915 920 925 Leu Lys Asn Asn 9302574 base pairs nucleic acid single linear other nucleic acid /desc =”DNA (genomic) (p10b30)“ CDS 1..2571 46 ATG CGT CTG GAT CGT CTT ACT AATAAA TTC CAG CTT GCT CTT GCC GAT 48 Met Arg Leu Asp Arg Leu Thr Asn LysPhe Gln Leu Ala Leu Ala Asp 1 5 10 15 GCC CAA TCA CTT GCA CTC GGG CACGAC AAC CAA TTT ATC GAA CCA CTT 96 Ala Gln Ser Leu Ala Leu Gly His AspAsn Gln Phe Ile Glu Pro Leu 20 25 30 CAT TTA ATG AGC GCC CTG CTG AAT CAGGAA GGG GGT TCG GTT AGT CCT 144 His Leu Met Ser Ala Leu Leu Asn Gln GluGly Gly Ser Val Ser Pro 35 40 45 TTA TTA ACA TCC GCT GGC ATA AAT GCT GGCCAG TTG CGC ACA GAT ATC 192 Leu Leu Thr Ser Ala Gly Ile Asn Ala Gly GlnLeu Arg Thr Asp Ile 50 55 60 AAT CAG GCA TTA AAT CGT TTA CCG CAG GTT GAAGGT ACT GGT GGT GAT 240 Asn Gln Ala Leu Asn Arg Leu Pro Gln Val Glu GlyThr Gly Gly Asp 65 70 75 80 GTC CAG CCA TCA CAG GAT CTG GTG CGC GTT CTTAAT CTT TGC GAC AAC 288 Val Gln Pro Ser Gln Asp Leu Val Arg Val Leu AsnLeu Cys Asp Asn 85 90 95 GTG GCG CAA AAA CGT GGT GAT AAC TTT ATC TCG TCAGAA CTG TTC GTT 336 Val Ala Gln Lys Arg Gly Asp Asn Phe Ile Ser Ser GluLeu Phe Val 100 105 110 CTG GCG GCA CTT GAG TCT CGC GGC ACC GTG GCC GACATC CTG AAA GCA 384 Leu Ala Ala Leu Glu Ser Arg Gly Thr Val Ala Asp IleLeu Lys Ala 115 120 125 GCA GGG GCG ACC ACC GCC AAC ATT ACT CAA GCG ATTGAA CAA ATG CGT 432 Ala Gly Ala Thr Thr Ala Asn Ile Thr Gln Ala Ile GluGln Met Arg 130 135 140 GGA GGT GAA AGC GTG AAC GAT CAA GGT GCT GAA GACCAA CGT CAG GCT 480 Gly Gly Glu Ser Val Asn Asp Gln Gly Ala Glu Asp GlnArg Gln Ala 145 150 155 160 TTG AAA AAA TAT ACC ATC GAC CTT ACC GAA CGAGCC GAA CAG GGC AAA 528 Leu Lys Lys Tyr Thr Ile Asp Leu Thr Glu Arg AlaGlu Gln Gly Lys 165 170 175 CTC GAT CCG GTG ATT GGT CGT GAT GAA GAA ATTCGC CGT ACC ATT CAG 576 Leu Asp Pro Val Ile Gly Arg Asp Glu Glu Ile ArgArg Thr Ile Gln 180 185 190 GTG CTG CAA CGT CGT ACT AAA AAT AAC CCG GTACTG ATT GGT GAA CCC 624 Val Leu Gln Arg Arg Thr Lys Asn Asn Pro Val LeuIle Gly Glu Pro 195 200 205 GGC GTC GGT AAA ACT GCC ATC GTT GAA GGT CTGGCG CAG CGT ATT ATC 672 Gly Val Gly Lys Thr Ala Ile Val Glu Gly Leu AlaGln Arg Ile Ile 210 215 220 AAC GGC GAA GTG CCG GAA GGG TTG AAA GGC CGCCGG GTA CTG GCG CTG 720 Asn Gly Glu Val Pro Glu Gly Leu Lys Gly Arg ArgVal Leu Ala Leu 225 230 235 240 GAT ATG GGC GCG CTG GTG GCT GGG GCG AAATAT CGC GGT GAG TTT GAA 768 Asp Met Gly Ala Leu Val Ala Gly Ala Lys TyrArg Gly Glu Phe Glu 245 250 255 GAA CGT TTA AAA GGC GTG CTT AAC GAT CTTGCC AAA CAG GAA GGC AAC 816 Glu Arg Leu Lys Gly Val Leu Asn Asp Leu AlaLys Gln Glu Gly Asn 260 265 270 GTC ATC CTA TTT ATC GAC GAA TTA CAT ACCATG GTC GGC GCG GGT AAA 864 Val Ile Leu Phe Ile Asp Glu Leu His Thr MetVal Gly Ala Gly Lys 275 280 285 GCC GAT GGC GCA ATG GAC GCC GGA AAC ATGCTG AAA CCG GCG CTG GCG 912 Ala Asp Gly Ala Met Asp Ala Gly Asn Met LeuLys Pro Ala Leu Ala 290 295 300 CGT GGT GAA TTG CAC TGC GTA GGT GCC ACGACG CTT GAC GAA TAT CGC 960 Arg Gly Glu Leu His Cys Val Gly Ala Thr ThrLeu Asp Glu Tyr Arg 305 310 315 320 CAG TAC ATT GAA AAA GAT GCT GCG CTGGAA CGT CGT TTC CAG AAA GTG 1008 Gln Tyr Ile Glu Lys Asp Ala Ala Leu GluArg Arg Phe Gln Lys Val 325 330 335 TTT GTT GCC GAG CCT TCT GTT GAA GATACC ATT GCG ATT CTG CGT GGC 1056 Phe Val Ala Glu Pro Ser Val Glu Asp ThrIle Ala Ile Leu Arg Gly 340 345 350 CTG AAA GAA CGT TAC GAA TTG CAC CACCAT GTG CAA ATT ACT GAC CCG 1104 Leu Lys Glu Arg Tyr Glu Leu His His HisVal Gln Ile Thr Asp Pro 355 360 365 GCA ATT GTT GCA GCG GCG ACG TTG TCTCAT CGC TAC ATT GCT GAC CGT 1152 Ala Ile Val Ala Ala Ala Thr Leu Ser HisArg Tyr Ile Ala Asp Arg 370 375 380 CAG CTG CCG GAT AAA GCC ATC GAC CTGATC GAT GAA GCA GCA TCC AGC 1200 Gln Leu Pro Asp Lys Ala Ile Asp Leu IleAsp Glu Ala Ala Ser Ser 385 390 395 400 ATT CGT ATG CAG ATT GAC TCA AAACCA GAA GAA CTC GAC CGA CTC GAT 1248 Ile Arg Met Gln Ile Asp Ser Lys ProGlu Glu Leu Asp Arg Leu Asp 405 410 415 CGT CGT ATC ATC CAG CTC AAA CTGGAA CAA CAG GCG TTA ATG AAA GAG 1296 Arg Arg Ile Ile Gln Leu Lys Leu GluGln Gln Ala Leu Met Lys Glu 420 425 430 TCT GAT GAA GCC AGT AAA AAA CGTCTG GAT ATG CTC AAC GAA GAA CTG 1344 Ser Asp Glu Ala Ser Lys Lys Arg LeuAsp Met Leu Asn Glu Glu Leu 435 440 445 AGC GAC AAA GAA CGT CAG TAC TCCGAG TTA GAA GAA GAG TGG AAA GCA 1392 Ser Asp Lys Glu Arg Gln Tyr Ser GluLeu Glu Glu Glu Trp Lys Ala 450 455 460 GAG AAG GCA TCG CTT TCT GGT ACGCAG ACC ATT AAA GCG GAA CTG GAA 1440 Glu Lys Ala Ser Leu Ser Gly Thr GlnThr Ile Lys Ala Glu Leu Glu 465 470 475 480 CAG GCG AAA ATC GCT ATT GAACAG GCT CGC CGT GTG GGG GAC CTG GCG 1488 Gln Ala Lys Ile Ala Ile Glu GlnAla Arg Arg Val Gly Asp Leu Ala 485 490 495 CGG ATG TCT GAA CTG CAA TACGGC AAA ATC CCG GAA CTG GAA AAG CAA 1536 Arg Met Ser Glu Leu Gln Tyr GlyLys Ile Pro Glu Leu Glu Lys Gln 500 505 510 CTG GAA GCC GCA ACG CAG CTCGAA GGC AAA ACT ATG CGT CTG TTG CGT 1584 Leu Glu Ala Ala Thr Gln Leu GluGly Lys Thr Met Arg Leu Leu Arg 515 520 525 AAT AAA GTG ACC GAC GCC GAAATT GCT GAA GTG CTG GCG CGT TGG ACG 1632 Asn Lys Val Thr Asp Ala Glu IleAla Glu Val Leu Ala Arg Trp Thr 530 535 540 GGG ATT CCG GTT TCT CGC ATGATG GAA AGC GAG CGC GAA AAA CTG CTG 1680 Gly Ile Pro Val Ser Arg Met MetGlu Ser Glu Arg Glu Lys Leu Leu 545 550 555 560 CGT ATG GAG CAA GAA CTGCAC CAT CGC GTA ATT GGT CAG AAC GAA GCG 1728 Arg Met Glu Gln Glu Leu HisHis Arg Val Ile Gly Gln Asn Glu Ala 565 570 575 GTT GAT GCG GTA TCT AACGCT ATT CGT CGT AGC CGT GCG GGG CTG GCG 1776 Val Asp Ala Val Ser Asn AlaIle Arg Arg Ser Arg Ala Gly Leu Ala 580 585 590 GAT CCA AAT CGC CCG ATTGGT TCA TTC CTG TTC CTC GGC CCA ACT GGT 1824 Asp Pro Asn Arg Pro Ile GlySer Phe Leu Phe Leu Gly Pro Thr Gly 595 600 605 GTG GGG AAA ACA GAG CTTTGT AAG GCG CTG GCG AAC TTT ATG TTT GAT 1872 Val Gly Lys Thr Glu Leu CysLys Ala Leu Ala Asn Phe Met Phe Asp 610 615 620 AGC GAC GAG GCG ATG GTCCGT ATC GAT ATG TCC GAG TTT ATG GAG AAA 1920 Ser Asp Glu Ala Met Val ArgIle Asp Met Ser Glu Phe Met Glu Lys 625 630 635 640 CAC TCG GTG TCT CGTTTG GTT GGT GCG CCT CCG GGA TAT GTC GGT TAT 1968 His Ser Val Ser Arg LeuVal Gly Ala Pro Pro Gly Tyr Val Gly Tyr 645 650 655 GAA GAA GGT GGC TACCTG ACC GAA GCG GTG CGT CGT CGT CCG TAT TCC 2016 Glu Glu Gly Gly Tyr LeuThr Glu Ala Val Arg Arg Arg Pro Tyr Ser 660 665 670 GTC ATC CTG CTG GATGAA GTG GAA AAA GCG CAT CCG GAT GTC TTC AAC 2064 Val Ile Leu Leu Asp GluVal Glu Lys Ala His Pro Asp Val Phe Asn 675 680 685 ATT CTG TTG CAG GTACTG GAT GAT GGG CGT CTG ACT GAC GGG CAA GGG 2112 Ile Leu Leu Gln Val LeuAsp Asp Gly Arg Leu Thr Asp Gly Gln Gly 690 695 700 AGA ACG GTC GAC TTCCGT AAT ACG GTC GTC ATT ATG ACC TCT AAC CTC 2160 Arg Thr Val Asp Phe ArgAsn Thr Val Val Ile Met Thr Ser Asn Leu 705 710 715 720 GGT TCC GAT CTGATT CAG GAA CGC TTC GGT GAA CTG GAT TAT GCG CAC 2208 Gly Ser Asp Leu IleGln Glu Arg Phe Gly Glu Leu Asp Tyr Ala His 725 730 735 ATG AAA GAG CTGGTG CTC GGT GTG GTA AGC CAT AAC TTC CGT CCG GAA 2256 Met Lys Glu Leu ValLeu Gly Val Val Ser His Asn Phe Arg Pro Glu 740 745 750 TTC ATT AAC CGTATC GAT GAA GTG GTG GTC TTC CAT CCG CTG GGT GAA 2304 Phe Ile Asn Arg IleAsp Glu Val Val Val Phe His Pro Leu Gly Glu 755 760 765 CAG CAC ATT GCCTCG ATT GCG CAG ATT CAG TTG AAA CGT CTG TAC AAA 2352 Gln His Ile Ala SerIle Ala Gln Ile Gln Leu Lys Arg Leu Tyr Lys 770 775 780 CGT CTG GAA GAACGT GGT TAT GAA ATC CAC ATT TCT GAC GAG GCG CTG 2400 Arg Leu Glu Glu ArgGly Tyr Glu Ile His Ile Ser Asp Glu Ala Leu 785 790 795 800 AAA CTG CTGAGC GAG AAC GGT TAC GAT CCG GTC TAT GGT GCA CGT CCT 2448 Lys Leu Leu SerGlu Asn Gly Tyr Asp Pro Val Tyr Gly Ala Arg Pro 805 810 815 CTG AAA CGTGCA ATT CAG CAG CAG ATC GAA AAC CCG CTG GCA CAG CAA 2496 Leu Lys Arg AlaIle Gln Gln Gln Ile Glu Asn Pro Leu Ala Gln Gln 820 825 830 ATA CTG TCTGGT GAA TTG GTT CCG GGT AAA GTG ATT CGC CTG GAA GTT 2544 Ile Leu Ser GlyGlu Leu Val Pro Gly Lys Val Ile Arg Leu Glu Val 835 840 845 AAT GAA GACCGG ATT GTC GCC GTC CAG TAA 2574 Asn Glu Asp Arg Ile Val Ala Val Gln 850855 857 amino acids amino acid linear protein 47 Met Arg Leu Asp Arg LeuThr Asn Lys Phe Gln Leu Ala Leu Ala Asp 1 5 10 15 Ala Gln Ser Leu AlaLeu Gly His Asp Asn Gln Phe Ile Glu Pro Leu 20 25 30 His Leu Met Ser AlaLeu Leu Asn Gln Glu Gly Gly Ser Val Ser Pro 35 40 45 Leu Leu Thr Ser AlaGly Ile Asn Ala Gly Gln Leu Arg Thr Asp Ile 50 55 60 Asn Gln Ala Leu AsnArg Leu Pro Gln Val Glu Gly Thr Gly Gly Asp 65 70 75 80 Val Gln Pro SerGln Asp Leu Val Arg Val Leu Asn Leu Cys Asp Asn 85 90 95 Val Ala Gln LysArg Gly Asp Asn Phe Ile Ser Ser Glu Leu Phe Val 100 105 110 Leu Ala AlaLeu Glu Ser Arg Gly Thr Val Ala Asp Ile Leu Lys Ala 115 120 125 Ala GlyAla Thr Thr Ala Asn Ile Thr Gln Ala Ile Glu Gln Met Arg 130 135 140 GlyGly Glu Ser Val Asn Asp Gln Gly Ala Glu Asp Gln Arg Gln Ala 145 150 155160 Leu Lys Lys Tyr Thr Ile Asp Leu Thr Glu Arg Ala Glu Gln Gly Lys 165170 175 Leu Asp Pro Val Ile Gly Arg Asp Glu Glu Ile Arg Arg Thr Ile Gln180 185 190 Val Leu Gln Arg Arg Thr Lys Asn Asn Pro Val Leu Ile Gly GluPro 195 200 205 Gly Val Gly Lys Thr Ala Ile Val Glu Gly Leu Ala Gln ArgIle Ile 210 215 220 Asn Gly Glu Val Pro Glu Gly Leu Lys Gly Arg Arg ValLeu Ala Leu 225 230 235 240 Asp Met Gly Ala Leu Val Ala Gly Ala Lys TyrArg Gly Glu Phe Glu 245 250 255 Glu Arg Leu Lys Gly Val Leu Asn Asp LeuAla Lys Gln Glu Gly Asn 260 265 270 Val Ile Leu Phe Ile Asp Glu Leu HisThr Met Val Gly Ala Gly Lys 275 280 285 Ala Asp Gly Ala Met Asp Ala GlyAsn Met Leu Lys Pro Ala Leu Ala 290 295 300 Arg Gly Glu Leu His Cys ValGly Ala Thr Thr Leu Asp Glu Tyr Arg 305 310 315 320 Gln Tyr Ile Glu LysAsp Ala Ala Leu Glu Arg Arg Phe Gln Lys Val 325 330 335 Phe Val Ala GluPro Ser Val Glu Asp Thr Ile Ala Ile Leu Arg Gly 340 345 350 Leu Lys GluArg Tyr Glu Leu His His His Val Gln Ile Thr Asp Pro 355 360 365 Ala IleVal Ala Ala Ala Thr Leu Ser His Arg Tyr Ile Ala Asp Arg 370 375 380 GlnLeu Pro Asp Lys Ala Ile Asp Leu Ile Asp Glu Ala Ala Ser Ser 385 390 395400 Ile Arg Met Gln Ile Asp Ser Lys Pro Glu Glu Leu Asp Arg Leu Asp 405410 415 Arg Arg Ile Ile Gln Leu Lys Leu Glu Gln Gln Ala Leu Met Lys Glu420 425 430 Ser Asp Glu Ala Ser Lys Lys Arg Leu Asp Met Leu Asn Glu GluLeu 435 440 445 Ser Asp Lys Glu Arg Gln Tyr Ser Glu Leu Glu Glu Glu TrpLys Ala 450 455 460 Glu Lys Ala Ser Leu Ser Gly Thr Gln Thr Ile Lys AlaGlu Leu Glu 465 470 475 480 Gln Ala Lys Ile Ala Ile Glu Gln Ala Arg ArgVal Gly Asp Leu Ala 485 490 495 Arg Met Ser Glu Leu Gln Tyr Gly Lys IlePro Glu Leu Glu Lys Gln 500 505 510 Leu Glu Ala Ala Thr Gln Leu Glu GlyLys Thr Met Arg Leu Leu Arg 515 520 525 Asn Lys Val Thr Asp Ala Glu IleAla Glu Val Leu Ala Arg Trp Thr 530 535 540 Gly Ile Pro Val Ser Arg MetMet Glu Ser Glu Arg Glu Lys Leu Leu 545 550 555 560 Arg Met Glu Gln GluLeu His His Arg Val Ile Gly Gln Asn Glu Ala 565 570 575 Val Asp Ala ValSer Asn Ala Ile Arg Arg Ser Arg Ala Gly Leu Ala 580 585 590 Asp Pro AsnArg Pro Ile Gly Ser Phe Leu Phe Leu Gly Pro Thr Gly 595 600 605 Val GlyLys Thr Glu Leu Cys Lys Ala Leu Ala Asn Phe Met Phe Asp 610 615 620 SerAsp Glu Ala Met Val Arg Ile Asp Met Ser Glu Phe Met Glu Lys 625 630 635640 His Ser Val Ser Arg Leu Val Gly Ala Pro Pro Gly Tyr Val Gly Tyr 645650 655 Glu Glu Gly Gly Tyr Leu Thr Glu Ala Val Arg Arg Arg Pro Tyr Ser660 665 670 Val Ile Leu Leu Asp Glu Val Glu Lys Ala His Pro Asp Val PheAsn 675 680 685 Ile Leu Leu Gln Val Leu Asp Asp Gly Arg Leu Thr Asp GlyGln Gly 690 695 700 Arg Thr Val Asp Phe Arg Asn Thr Val Val Ile Met ThrSer Asn Leu 705 710 715 720 Gly Ser Asp Leu Ile Gln Glu Arg Phe Gly GluLeu Asp Tyr Ala His 725 730 735 Met Lys Glu Leu Val Leu Gly Val Val SerHis Asn Phe Arg Pro Glu 740 745 750 Phe Ile Asn Arg Ile Asp Glu Val ValVal Phe His Pro Leu Gly Glu 755 760 765 Gln His Ile Ala Ser Ile Ala GlnIle Gln Leu Lys Arg Leu Tyr Lys 770 775 780 Arg Leu Glu Glu Arg Gly TyrGlu Ile His Ile Ser Asp Glu Ala Leu 785 790 795 800 Lys Leu Leu Ser GluAsn Gly Tyr Asp Pro Val Tyr Gly Ala Arg Pro 805 810 815 Leu Lys Arg AlaIle Gln Gln Gln Ile Glu Asn Pro Leu Ala Gln Gln 820 825 830 Ile Leu SerGly Glu Leu Val Pro Gly Lys Val Ile Arg Leu Glu Val 835 840 845 Asn GluAsp Arg Ile Val Ala Val Gln 850 855 1239 base pairs nucleic acid singlelinear other nucleic acid /desc = ”DNA (genomic) (p13c3)“ CDS 1..1236 48ATG ATG AAA GAA AAA GTG ATT TTT CTC GTT GAC ATG CAA TCG TTT TAT 48 MetMet Lys Glu Lys Val Ile Phe Leu Val Asp Met Gln Ser Phe Tyr 1 5 10 15GCA TCT GTA GAG AAA GCG GAA AAT CCA CAT TTG AAA AAT AGG CCC GTC 96 AlaSer Val Glu Lys Ala Glu Asn Pro His Leu Lys Asn Arg Pro Val 20 25 30 ATTGTT TCG GGT GAC CCT GAA AAA AGG GGC GGA GTC GTA TTG GCT GCC 144 Ile ValSer Gly Asp Pro Glu Lys Arg Gly Gly Val Val Leu Ala Ala 35 40 45 TGC CCGCTG GCG AAA CAA AAG GGT GTG GTG AAT GCT TCA CGG CTG TGG 192 Cys Pro LeuAla Lys Gln Lys Gly Val Val Asn Ala Ser Arg Leu Trp 50 55 60 GAG GCG CAGGAA AAG TGT CCT GAG GCT GTT GTG CTC CGG CCG CGT ATG 240 Glu Ala Gln GluLys Cys Pro Glu Ala Val Val Leu Arg Pro Arg Met 65 70 75 80 CAG CGG TATATT GAT GTA TCA CTG CAA ATT ACG GCC ATT CTC GAG GAG 288 Gln Arg Tyr IleAsp Val Ser Leu Gln Ile Thr Ala Ile Leu Glu Glu 85 90 95 TAT ACA GAC CTTGTG GAG CCG TAT TCC ATC GAT GAA CAG TTC ATG GAC 336 Tyr Thr Asp Leu ValGlu Pro Tyr Ser Ile Asp Glu Gln Phe Met Asp 100 105 110 ATT ACA GGC AGCCAG AAG CTG TTT GGG ACG CCG ATG GAG ATC GCG AAA 384 Ile Thr Gly Ser GlnLys Leu Phe Gly Thr Pro Met Glu Ile Ala Lys 115 120 125 AGC ATT CAG GGCAGA ATC ATG CGG GAG ATC GGC GTT TAT GCA CGG GTC 432 Ser Ile Gln Gly ArgIle Met Arg Glu Ile Gly Val Tyr Ala Arg Val 130 135 140 GGA ATC GGC CCTAAC AAA GCG CTG GCC AAA ATT GCG TGT GAC AAT TTT 480 Gly Ile Gly Pro AsnLys Ala Leu Ala Lys Ile Ala Cys Asp Asn Phe 145 150 155 160 GCC AAA AAGAAT AAG AAC GGT ATT TTT ACC TTA ACG AAA GAA AAT ATG 528 Ala Lys Lys AsnLys Asn Gly Ile Phe Thr Leu Thr Lys Glu Asn Met 165 170 175 AAA ACC GAAATG TGG CCG CTC CCG GTG GGC AGC ATG TTT GGC GTC GGG 576 Lys Thr Glu MetTrp Pro Leu Pro Val Gly Ser Met Phe Gly Val Gly 180 185 190 AGC CGC ATGAAG CAT CAT TTA AAT CGA ATG GGC ATC AGC ACG ATC GGC 624 Ser Arg Met LysHis His Leu Asn Arg Met Gly Ile Ser Thr Ile Gly 195 200 205 GGG CTC GCGGCT TTT CCG CTC GAT CTT TTA AAA AAG AAA TGG GGC ATT 672 Gly Leu Ala AlaPhe Pro Leu Asp Leu Leu Lys Lys Lys Trp Gly Ile 210 215 220 AAC GGC CACGTG CTG TGG ATG ACG GCA AAC GGA ATC GAC TAT TCC CCT 720 Asn Gly His ValLeu Trp Met Thr Ala Asn Gly Ile Asp Tyr Ser Pro 225 230 235 240 GTG TCAACT TCG TCT CTG GAC GGG CAA AAG GCG ATA GGT CAT GGA ATG 768 Val Ser ThrSer Ser Leu Asp Gly Gln Lys Ala Ile Gly His Gly Met 245 250 255 ACT CTCCCG AGA GAC TAC GAA CAC TTT GAC AAA GAA ATC AAG GTC GTA 816 Thr Leu ProArg Asp Tyr Glu His Phe Asp Lys Glu Ile Lys Val Val 260 265 270 TTG CTTGAG CTG AGT GAA GAG GTG TGC AGG CGA AGC CGA AAC GCC GGG 864 Leu Leu GluLeu Ser Glu Glu Val Cys Arg Arg Ser Arg Asn Ala Gly 275 280 285 GTC ATGGGG CAG ACA GTG TCA GTG AGC TGC CGG GGT GCT GAT TTT GAT 912 Val Met GlyGln Thr Val Ser Val Ser Cys Arg Gly Ala Asp Phe Asp 290 295 300 TGG CCGACG GGC TTC AAC CGG CAA GTG AAG CTG GCA GAG CCG ACT AAT 960 Trp Pro ThrGly Phe Asn Arg Gln Val Lys Leu Ala Glu Pro Thr Asn 305 310 315 320 TCTACG CAG GAT GTA TAT GAG GCT GTA CGA CGG CTG TTT CTT ACA TTT 1008 Ser ThrGln Asp Val Tyr Glu Ala Val Arg Arg Leu Phe Leu Thr Phe 325 330 335 TGGGAC GGG AAA CCC GTC CGC CGC CTC GGT GTC AAT CTG TCT CAG CTC 1056 Trp AspGly Lys Pro Val Arg Arg Leu Gly Val Asn Leu Ser Gln Leu 340 345 350 TCATCT GAT GAC ATA TGG CAG CTC AAT TTA TTT CAG GAT TAT GCA AAG 1104 Ser SerAsp Asp Ile Trp Gln Leu Asn Leu Phe Gln Asp Tyr Ala Lys 355 360 365 AAAATG AGC CTA GGC TAT GTG ATG GAT GGC ATT AAA AAT CGA TTC GGC 1152 Lys MetSer Leu Gly Tyr Val Met Asp Gly Ile Lys Asn Arg Phe Gly 370 375 380 GATACA GCA ATC ATC AGG GCG GCG TCA CTG ACA GCG GCA GGC CAG GCA 1200 Asp ThrAla Ile Ile Arg Ala Ala Ser Leu Thr Ala Ala Gly Gln Ala 385 390 395 400TTT GAA CGT GCG GCT AAA ATA GGG GGG CAT TAT AAA TGA 1239 Phe Glu Arg AlaAla Lys Ile Gly Gly His Tyr Lys 405 410 412 amino acids amino acidlinear protein 49 Met Met Lys Glu Lys Val Ile Phe Leu Val Asp Met GlnSer Phe Tyr 1 5 10 15 Ala Ser Val Glu Lys Ala Glu Asn Pro His Leu LysAsn Arg Pro Val 20 25 30 Ile Val Ser Gly Asp Pro Glu Lys Arg Gly Gly ValVal Leu Ala Ala 35 40 45 Cys Pro Leu Ala Lys Gln Lys Gly Val Val Asn AlaSer Arg Leu Trp 50 55 60 Glu Ala Gln Glu Lys Cys Pro Glu Ala Val Val LeuArg Pro Arg Met 65 70 75 80 Gln Arg Tyr Ile Asp Val Ser Leu Gln Ile ThrAla Ile Leu Glu Glu 85 90 95 Tyr Thr Asp Leu Val Glu Pro Tyr Ser Ile AspGlu Gln Phe Met Asp 100 105 110 Ile Thr Gly Ser Gln Lys Leu Phe Gly ThrPro Met Glu Ile Ala Lys 115 120 125 Ser Ile Gln Gly Arg Ile Met Arg GluIle Gly Val Tyr Ala Arg Val 130 135 140 Gly Ile Gly Pro Asn Lys Ala LeuAla Lys Ile Ala Cys Asp Asn Phe 145 150 155 160 Ala Lys Lys Asn Lys AsnGly Ile Phe Thr Leu Thr Lys Glu Asn Met 165 170 175 Lys Thr Glu Met TrpPro Leu Pro Val Gly Ser Met Phe Gly Val Gly 180 185 190 Ser Arg Met LysHis His Leu Asn Arg Met Gly Ile Ser Thr Ile Gly 195 200 205 Gly Leu AlaAla Phe Pro Leu Asp Leu Leu Lys Lys Lys Trp Gly Ile 210 215 220 Asn GlyHis Val Leu Trp Met Thr Ala Asn Gly Ile Asp Tyr Ser Pro 225 230 235 240Val Ser Thr Ser Ser Leu Asp Gly Gln Lys Ala Ile Gly His Gly Met 245 250255 Thr Leu Pro Arg Asp Tyr Glu His Phe Asp Lys Glu Ile Lys Val Val 260265 270 Leu Leu Glu Leu Ser Glu Glu Val Cys Arg Arg Ser Arg Asn Ala Gly275 280 285 Val Met Gly Gln Thr Val Ser Val Ser Cys Arg Gly Ala Asp PheAsp 290 295 300 Trp Pro Thr Gly Phe Asn Arg Gln Val Lys Leu Ala Glu ProThr Asn 305 310 315 320 Ser Thr Gln Asp Val Tyr Glu Ala Val Arg Arg LeuPhe Leu Thr Phe 325 330 335 Trp Asp Gly Lys Pro Val Arg Arg Leu Gly ValAsn Leu Ser Gln Leu 340 345 350 Ser Ser Asp Asp Ile Trp Gln Leu Asn LeuPhe Gln Asp Tyr Ala Lys 355 360 365 Lys Met Ser Leu Gly Tyr Val Met AspGly Ile Lys Asn Arg Phe Gly 370 375 380 Asp Thr Ala Ile Ile Arg Ala AlaSer Leu Thr Ala Ala Gly Gln Ala 385 390 395 400 Phe Glu Arg Ala Ala LysIle Gly Gly His Tyr Lys 405 410 1452 base pairs nucleic acid singlelinear other nucleic acid /desc = ”DNA (genomic) (p4b3)“ CDS 1..1449 50ATG AGT AAA ATT ATT GGA TCA GAC AGA GTC AAA AGA GGT ATG GCT GAA 48 MetSer Lys Ile Ile Gly Ser Asp Arg Val Lys Arg Gly Met Ala Glu 1 5 10 15ATG CAA AAA GGC GGC GTT ATT ATG GAT GTC GTT AAT GCT GAG CAA GCA 96 MetGln Lys Gly Gly Val Ile Met Asp Val Val Asn Ala Glu Gln Ala 20 25 30 AGAATT GCA GAA GAA GCT GGC GCG GTA GCA GTT ATG GCA TTA GAA CGA 144 Arg IleAla Glu Glu Ala Gly Ala Val Ala Val Met Ala Leu Glu Arg 35 40 45 GTA CCTTCT GAT ATT AGA GCT GCT GGT GGT GTT GCA CGT ATG GCA AAC 192 Val Pro SerAsp Ile Arg Ala Ala Gly Gly Val Ala Arg Met Ala Asn 50 55 60 CCT AAA ATTGTA GAA GAA GTA ATG AAT GCT GTT TCT ATT CCA GTC ATG 240 Pro Lys Ile ValGlu Glu Val Met Asn Ala Val Ser Ile Pro Val Met 65 70 75 80 GCT AAA GCACGT ATT GGT CAT ATC ACT GAA GCA AGA GTA TTA GAG GCG 288 Ala Lys Ala ArgIle Gly His Ile Thr Glu Ala Arg Val Leu Glu Ala 85 90 95 ATG GGT GTT GACTAT ATT GAT GAA TCA GAA GTG TTA ACA CCA GCA GAT 336 Met Gly Val Asp TyrIle Asp Glu Ser Glu Val Leu Thr Pro Ala Asp 100 105 110 GAG GAA TAT CACTTA AGA AAA GAT CAA TTT ACA GTA CCA TTT GTA TGT 384 Glu Glu Tyr His LeuArg Lys Asp Gln Phe Thr Val Pro Phe Val Cys 115 120 125 GGA TGT CGT AATTTA GGT GAA GMT GCG CGT AGA ATT GGT GAA GGT GCT 432 Gly Cys Arg Asn LeuGly Glu Xaa Ala Arg Arg Ile Gly Glu Gly Ala 130 135 140 GCT ATG TTA CGTACT AAA GGT GAA CCA GGT ACA GGT AAT ATT GTT GAA 480 Ala Met Leu Arg ThrLys Gly Glu Pro Gly Thr Gly Asn Ile Val Glu 145 150 155 160 GCT GTA AGACAT ATG AGA CAA GTT AAT TCA GAA GTT AGT CGA TTG ACT 528 Ala Val Arg HisMet Arg Gln Val Asn Ser Glu Val Ser Arg Leu Thr 165 170 175 GTA ATG AATGAT GAT GAG ATT ATG ACT TTT GCG AAA GAT ATC GGT GCG 576 Val Met Asn AspAsp Glu Ile Met Thr Phe Ala Lys Asp Ile Gly Ala 180 185 190 CCT TAT GAAATT TTA AAA CAA ATT AAA GAC AAT GGT CGT TTA CCG GTA 624 Pro Tyr Glu IleLeu Lys Gln Ile Lys Asp Asn Gly Arg Leu Pro Val 195 200 205 GTT AAC TTTGCA GCT GGT GGC GTT GCG ACT CCT CAA GAT GCT GCT TTA 672 Val Asn Phe AlaAla Gly Gly Val Ala Thr Pro Gln Asp Ala Ala Leu 210 215 220 ATG ATG GAATTA GGT GCT GAC GGT GTA TTC GTT GGA TCA GGT ATT TTT 720 Met Met Glu LeuGly Ala Asp Gly Val Phe Val Gly Ser Gly Ile Phe 225 230 235 240 AAA TCAGAA GAT CCA GAA AAA TTT GCT AAA GCA ATT GTT CAA GCA ACA 768 Lys Ser GluAsp Pro Glu Lys Phe Ala Lys Ala Ile Val Gln Ala Thr 245 250 255 ACA CATTAC CAA GAC TAT GAA CTA ATT GGA AGA TTA GCA AGT GAA CTT 816 Thr His TyrGln Asp Tyr Glu Leu Ile Gly Arg Leu Ala Ser Glu Leu 260 265 270 GGC ACTGCT ATG AAA GGT TTA GAT ATC AAT CAA TTA TCA TTA GAA GAA 864 Gly Thr AlaMet Lys Gly Leu Asp Ile Asn Gln Leu Ser Leu Glu Glu 275 280 285 CGT ATGCAA GAG CGT GGT TGG TAA GAT ATG AAA ATA GGT GTA TTA GCA 912 Arg Met GlnGlu Arg Gly Trp Xaa Asp Met Lys Ile Gly Val Leu Ala 290 295 300 TTA CAAGGT GCA GTA CGT GAA CAT ATT AGA CAT ATT GAA TTA AGT GGT 960 Leu Gln GlyAla Val Arg Glu His Ile Arg His Ile Glu Leu Ser Gly 305 310 315 320 CATGAA GGT ATT GCA GTT AAA AAA GTT GAA CAA TTA GAA GAA ATC GAG 1008 His GluGly Ile Ala Val Lys Lys Val Glu Gln Leu Glu Glu Ile Glu 325 330 335 GGCTTA ATA TTA CCT GGT GGC GAG TCT ACA ACG TTA CGT CGA TTA ATG 1056 Gly LeuIle Leu Pro Gly Gly Glu Ser Thr Thr Leu Arg Arg Leu Met 340 345 350 AATTTA TAT GGA TTT AAA GAG GCT TTA CAA AAT TCA ACT TTA CCT ATG 1104 Asn LeuTyr Gly Phe Lys Glu Ala Leu Gln Asn Ser Thr Leu Pro Met 355 360 365 TTTGGT ACA TGC GCA GGA TTA ATA GTT CTA GCG CAA GAT ATA GTT GGT 1152 Phe GlyThr Cys Ala Gly Leu Ile Val Leu Ala Gln Asp Ile Val Gly 370 375 380 GAAGAA GGA TAC CTT AAC AAG TTG AAT ATT ACT GTA CAA CGA AAC TCA 1200 Glu GluGly Tyr Leu Asn Lys Leu Asn Ile Thr Val Gln Arg Asn Ser 385 390 395 400TTC GGT AGA CAA GTT GAC AGC TTT GAA ACA GAA TTA GAT ATT AAA GGT 1248 PheGly Arg Gln Val Asp Ser Phe Glu Thr Glu Leu Asp Ile Lys Gly 405 410 415ATC GCT ACA GAT ATT GAA GGT GTC TTT ATA AGA GCC CCA CAT ATT GAA 1296 IleAla Thr Asp Ile Glu Gly Val Phe Ile Arg Ala Pro His Ile Glu 420 425 430AAA GTA GGT CAA GGC GTA GAT ATC CTA TGT AAG GTT AAT GAG AAA ATT 1344 LysVal Gly Gln Gly Val Asp Ile Leu Cys Lys Val Asn Glu Lys Ile 435 440 445GTA GCT GTT CAG CAA GGT AAA TAT TTA GGC GTA TCA TTC CAT CCT GAA 1392 ValAla Val Gln Gln Gly Lys Tyr Leu Gly Val Ser Phe His Pro Glu 450 455 460TTA ACA GAT GAC TAT AGA GTA ACT GAT TAC TTT ATT AAT CAT ATT GTA 1440 LeuThr Asp Asp Tyr Arg Val Thr Asp Tyr Phe Ile Asn His Ile Val 465 470 475480 AAA AAA GCA TAG 1452 Lys Lys Ala 483 amino acids amino acid linearprotein 51 Met Ser Lys Ile Ile Gly Ser Asp Arg Val Lys Arg Gly Met AlaGlu 1 5 10 15 Met Gln Lys Gly Gly Val Ile Met Asp Val Val Asn Ala GluGln Ala 20 25 30 Arg Ile Ala Glu Glu Ala Gly Ala Val Ala Val Met Ala LeuGlu Arg 35 40 45 Val Pro Ser Asp Ile Arg Ala Ala Gly Gly Val Ala Arg MetAla Asn 50 55 60 Pro Lys Ile Val Glu Glu Val Met Asn Ala Val Ser Ile ProVal Met 65 70 75 80 Ala Lys Ala Arg Ile Gly His Ile Thr Glu Ala Arg ValLeu Glu Ala 85 90 95 Met Gly Val Asp Tyr Ile Asp Glu Ser Glu Val Leu ThrPro Ala Asp 100 105 110 Glu Glu Tyr His Leu Arg Lys Asp Gln Phe Thr ValPro Phe Val Cys 115 120 125 Gly Cys Arg Asn Leu Gly Glu Xaa Ala Arg ArgIle Gly Glu Gly Ala 130 135 140 Ala Met Leu Arg Thr Lys Gly Glu Pro GlyThr Gly Asn Ile Val Glu 145 150 155 160 Ala Val Arg His Met Arg Gln ValAsn Ser Glu Val Ser Arg Leu Thr 165 170 175 Val Met Asn Asp Asp Glu IleMet Thr Phe Ala Lys Asp Ile Gly Ala 180 185 190 Pro Tyr Glu Ile Leu LysGln Ile Lys Asp Asn Gly Arg Leu Pro Val 195 200 205 Val Asn Phe Ala AlaGly Gly Val Ala Thr Pro Gln Asp Ala Ala Leu 210 215 220 Met Met Glu LeuGly Ala Asp Gly Val Phe Val Gly Ser Gly Ile Phe 225 230 235 240 Lys SerGlu Asp Pro Glu Lys Phe Ala Lys Ala Ile Val Gln Ala Thr 245 250 255 ThrHis Tyr Gln Asp Tyr Glu Leu Ile Gly Arg Leu Ala Ser Glu Leu 260 265 270Gly Thr Ala Met Lys Gly Leu Asp Ile Asn Gln Leu Ser Leu Glu Glu 275 280285 Arg Met Gln Glu Arg Gly Trp Xaa Asp Met Lys Ile Gly Val Leu Ala 290295 300 Leu Gln Gly Ala Val Arg Glu His Ile Arg His Ile Glu Leu Ser Gly305 310 315 320 His Glu Gly Ile Ala Val Lys Lys Val Glu Gln Leu Glu GluIle Glu 325 330 335 Gly Leu Ile Leu Pro Gly Gly Glu Ser Thr Thr Leu ArgArg Leu Met 340 345 350 Asn Leu Tyr Gly Phe Lys Glu Ala Leu Gln Asn SerThr Leu Pro Met 355 360 365 Phe Gly Thr Cys Ala Gly Leu Ile Val Leu AlaGln Asp Ile Val Gly 370 375 380 Glu Glu Gly Tyr Leu Asn Lys Leu Asn IleThr Val Gln Arg Asn Ser 385 390 395 400 Phe Gly Arg Gln Val Asp Ser PheGlu Thr Glu Leu Asp Ile Lys Gly 405 410 415 Ile Ala Thr Asp Ile Glu GlyVal Phe Ile Arg Ala Pro His Ile Glu 420 425 430 Lys Val Gly Gln Gly ValAsp Ile Leu Cys Lys Val Asn Glu Lys Ile 435 440 445 Val Ala Val Gln GlnGly Lys Tyr Leu Gly Val Ser Phe His Pro Glu 450 455 460 Leu Thr Asp AspTyr Arg Val Thr Asp Tyr Phe Ile Asn His Ile Val 465 470 475 480 Lys LysAla 977 base pairs nucleic acid single linear other nucleic acid /desc =”DNA (genomic) (p4c63)“ CDS 1..975 52 AAC AAA GCC TTC CAA TTA TCT GCGTCG GTA GAA CAA GTA TTA GCA ACT 48 Asn Lys Ala Phe Gln Leu Ser Ala SerVal Glu Gln Val Leu Ala Thr 1 5 10 15 TTA TCA CCT ACG CTA AAC AGT CCTTAC GAT TTA TAC GGC ACG ACA AAA 96 Leu Ser Pro Thr Leu Asn Ser Pro TyrAsp Leu Tyr Gly Thr Thr Lys 20 25 30 ATG CTA GAT ATT ACA TTC GAT TCA TTTGAA CAT GAT GGT ACA ACG TAC 144 Met Leu Asp Ile Thr Phe Asp Ser Phe GluHis Asp Gly Thr Thr Tyr 35 40 45 CCT GTC GAC TAT GCT ACG TTT GAA AAT GATTAT GAA GAT AAT AAA GAT 192 Pro Val Asp Tyr Ala Thr Phe Glu Asn Asp TyrGlu Asp Asn Lys Asp 50 55 60 CCT GAG TTT AGA CGT AAA AGT TTC AAA TCG TTTAGC GAT GGG ATT CGA 240 Pro Glu Phe Arg Arg Lys Ser Phe Lys Ser Phe SerAsp Gly Ile Arg 65 70 75 80 AAA TAT CAG CAT ACT ACC GCG GCT ACA TAT AATATG CAA GTA CAA CAA 288 Lys Tyr Gln His Thr Thr Ala Ala Thr Tyr Asn MetGln Val Gln Gln 85 90 95 GAA AAA ATT GAA GCT GAT TTA CGT GGA TTT GAA TCAGTC ATC GAT TAT 336 Glu Lys Ile Glu Ala Asp Leu Arg Gly Phe Glu Ser ValIle Asp Tyr 100 105 110 TTA TTA CAT AGT CAA GAA GTA ACG CGT GAT ATG TTTGAC CGT CAA ATC 384 Leu Leu His Ser Gln Glu Val Thr Arg Asp Met Phe AspArg Gln Ile 115 120 125 GAT ATG ATT ATG CGT GAC TTG GCA CCA GTT ATG CAGAAA TAT GCT AAA 432 Asp Met Ile Met Arg Asp Leu Ala Pro Val Met Gln LysTyr Ala Lys 130 135 140 CTT TTA CAA CGT ATT CAC GGA TTA GAT AAC ATG CGTTTT GAA GAC TTG 480 Leu Leu Gln Arg Ile His Gly Leu Asp Asn Met Arg PheGlu Asp Leu 145 150 155 160 AAG ATT TCT GTA GAC CCT GAT TAT GAA CCA GAGATT TCA ATT GAA GAC 528 Lys Ile Ser Val Asp Pro Asp Tyr Glu Pro Glu IleSer Ile Glu Asp 165 170 175 TCA AAA AAT TAT ATT TTC GGT GCG TTA AGT GTTTTA GGT GAT GAC TAT 576 Ser Lys Asn Tyr Ile Phe Gly Ala Leu Ser Val LeuGly Asp Asp Tyr 180 185 190 ACA AAC ATG TTA CGT GAA GCA TAC GAT CAG CGATGG ATT GAT TTT GCA 624 Thr Asn Met Leu Arg Glu Ala Tyr Asp Gln Arg TrpIle Asp Phe Ala 195 200 205 CAA AAT AAA GGT AAA GAT ACA GGC GCA TTT TGTGCA AGT CCA TAC TTT 672 Gln Asn Lys Gly Lys Asp Thr Gly Ala Phe Cys AlaSer Pro Tyr Phe 210 215 220 ACA CAT TCA TAT GTG TTT ATT TCT TGG ACT GGTAAA ATG GCT GAA GCA 720 Thr His Ser Tyr Val Phe Ile Ser Trp Thr Gly LysMet Ala Glu Ala 225 230 235 240 TTT GTC TTA GCA CAT GAA TTA GGT CAT GCAGGT CAT TTT ACA TTA GCT 768 Phe Val Leu Ala His Glu Leu Gly His Ala GlyHis Phe Thr Leu Ala 245 250 255 CAA AAA CAT CAA CCA TAT CTT GAA TCA GAAGCA TCA ATG TAC TTT GTT 816 Gln Lys His Gln Pro Tyr Leu Glu Ser Glu AlaSer Met Tyr Phe Val 260 265 270 GAA GCC CCT TCT ACA ATG AAT GAA ATG TTGATG GCC AAT TAT TTA TTT 864 Glu Ala Pro Ser Thr Met Asn Glu Met Leu MetAla Asn Tyr Leu Phe 275 280 285 AAC ACA AGT GAT AAT CCA AGA TTT AAG CGTTGG GTT ATT GGC TCA ATT 912 Asn Thr Ser Asp Asn Pro Arg Phe Lys Arg TrpVal Ile Gly Ser Ile 290 295 300 TTA TCT AGA ACA TAT TAT CAT AAT ATG GTACCC MTT TAT TAG AAG CNG 960 Leu Ser Arg Thr Tyr Tyr His Asn Met Val ProXaa Tyr Xaa Lys Xaa 305 310 315 320 CTT ATC CAC GGG GAG TG 977 Leu IleHis Gly Glu 325 325 amino acids amino acid linear protein 53 Asn Lys AlaPhe Gln Leu Ser Ala Ser Val Glu Gln Val Leu Ala Thr 1 5 10 15 Leu SerPro Thr Leu Asn Ser Pro Tyr Asp Leu Tyr Gly Thr Thr Lys 20 25 30 Met LeuAsp Ile Thr Phe Asp Ser Phe Glu His Asp Gly Thr Thr Tyr 35 40 45 Pro ValAsp Tyr Ala Thr Phe Glu Asn Asp Tyr Glu Asp Asn Lys Asp 50 55 60 Pro GluPhe Arg Arg Lys Ser Phe Lys Ser Phe Ser Asp Gly Ile Arg 65 70 75 80 LysTyr Gln His Thr Thr Ala Ala Thr Tyr Asn Met Gln Val Gln Gln 85 90 95 GluLys Ile Glu Ala Asp Leu Arg Gly Phe Glu Ser Val Ile Asp Tyr 100 105 110Leu Leu His Ser Gln Glu Val Thr Arg Asp Met Phe Asp Arg Gln Ile 115 120125 Asp Met Ile Met Arg Asp Leu Ala Pro Val Met Gln Lys Tyr Ala Lys 130135 140 Leu Leu Gln Arg Ile His Gly Leu Asp Asn Met Arg Phe Glu Asp Leu145 150 155 160 Lys Ile Ser Val Asp Pro Asp Tyr Glu Pro Glu Ile Ser IleGlu Asp 165 170 175 Ser Lys Asn Tyr Ile Phe Gly Ala Leu Ser Val Leu GlyAsp Asp Tyr 180 185 190 Thr Asn Met Leu Arg Glu Ala Tyr Asp Gln Arg TrpIle Asp Phe Ala 195 200 205 Gln Asn Lys Gly Lys Asp Thr Gly Ala Phe CysAla Ser Pro Tyr Phe 210 215 220 Thr His Ser Tyr Val Phe Ile Ser Trp ThrGly Lys Met Ala Glu Ala 225 230 235 240 Phe Val Leu Ala His Glu Leu GlyHis Ala Gly His Phe Thr Leu Ala 245 250 255 Gln Lys His Gln Pro Tyr LeuGlu Ser Glu Ala Ser Met Tyr Phe Val 260 265 270 Glu Ala Pro Ser Thr MetAsn Glu Met Leu Met Ala Asn Tyr Leu Phe 275 280 285 Asn Thr Ser Asp AsnPro Arg Phe Lys Arg Trp Val Ile Gly Ser Ile 290 295 300 Leu Ser Arg ThrTyr Tyr His Asn Met Val Pro Xaa Tyr Xaa Lys Xaa 305 310 315 320 Leu IleHis Gly Glu 325 471 base pairs nucleic acid single linear other nucleicacid /desc = ”DNA (genomic) (p5c3)“ CDS 1..465 54 ATG TAT CAA CTA CAATTT ATA AAT TTA GTT TAC GAC ACA ACC AAA CTC 48 Met Tyr Gln Leu Gln PheIle Asn Leu Val Tyr Asp Thr Thr Lys Leu 1 5 10 15 ACA CAT CTA GAA CAAACC AAT ATC AAT TTA TTC ATT GGT AAT TGG AGT 96 Thr His Leu Glu Gln ThrAsn Ile Asn Leu Phe Ile Gly Asn Trp Ser 20 25 30 AAT CAT CAA TTA CAA AAATCA ATT TGT ATA CGT CAT GGC GAT GAT ACA 144 Asn His Gln Leu Gln Lys SerIle Cys Ile Arg His Gly Asp Asp Thr 35 40 45 AGT CAC AAT CAA TAT CAT ATTCTT TTT ATA GAT ACG GCA CAT CAA CGC 192 Ser His Asn Gln Tyr His Ile LeuPhe Ile Asp Thr Ala His Gln Arg 50 55 60 ATT AAA TTT TCA TCT ATT GAT AATGAA GAA ATC ATT TAT ATT CTT GAT 240 Ile Lys Phe Ser Ser Ile Asp Asn GluGlu Ile Ile Tyr Ile Leu Asp 65 70 75 80 TAT GAT GAT ACA CAG CAT ATC CTCATG CAA ACG TCA TCC AAA CAA GGT 288 Tyr Asp Asp Thr Gln His Ile Leu MetGln Thr Ser Ser Lys Gln Gly 85 90 95 ATT GGC ACT TCG CGA CCA ATC GTT TATGAG CGC TTA GTA TAA CTA ATT 336 Ile Gly Thr Ser Arg Pro Ile Val Tyr GluArg Leu Val Xaa Leu Ile 100 105 110 TAA ATG ATT TCA CTT CAT AAA GCG GGTTGG CGA GAA TTC AAT TTC TCA 384 Xaa Met Ile Ser Leu His Lys Ala Gly TrpArg Glu Phe Asn Phe Ser 115 120 125 CCA GCT CGT TTT TTC ATT GTA ATA ATAATC TTT AAC ATT TAT TCT TTC 432 Pro Ala Arg Phe Phe Ile Val Ile Ile IlePhe Asn Ile Tyr Ser Phe 130 135 140 TCT ATT AAT TTT TCT CAA ACT ATC TTATCT TTA TGATAA 471 Ser Ile Asn Phe Ser Gln Thr Ile Leu Ser Leu 145 150155 155 amino acids amino acid linear protein 55 Met Tyr Gln Leu Gln PheIle Asn Leu Val Tyr Asp Thr Thr Lys Leu 1 5 10 15 Thr His Leu Glu GlnThr Asn Ile Asn Leu Phe Ile Gly Asn Trp Ser 20 25 30 Asn His Gln Leu GlnLys Ser Ile Cys Ile Arg His Gly Asp Asp Thr 35 40 45 Ser His Asn Gln TyrHis Ile Leu Phe Ile Asp Thr Ala His Gln Arg 50 55 60 Ile Lys Phe Ser SerIle Asp Asn Glu Glu Ile Ile Tyr Ile Leu Asp 65 70 75 80 Tyr Asp Asp ThrGln His Ile Leu Met Gln Thr Ser Ser Lys Gln Gly 85 90 95 Ile Gly Thr SerArg Pro Ile Val Tyr Glu Arg Leu Val Xaa Leu Ile 100 105 110 Xaa Met IleSer Leu His Lys Ala Gly Trp Arg Glu Phe Asn Phe Ser 115 120 125 Pro AlaArg Phe Phe Ile Val Ile Ile Ile Phe Asn Ile Tyr Ser Phe 130 135 140 SerIle Asn Phe Ser Gln Thr Ile Leu Ser Leu 145 150 155 588 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p8d26)“ CDS 14..343 56 ATTTNTAGAA TAA TAT TTC CAT ATT GGA AAA AGG GAAGAA TTC GTT ATG 49 Tyr Phe His Ile Gly Lys Arg Glu Glu Phe Val Met 1 510 AAA AGC TAT AAG TGT AAA GGT TCA TTC TTA ATA GAT AGT ATG GCT GGA 97Lys Ser Tyr Lys Cys Lys Gly Ser Phe Leu Ile Asp Ser Met Ala Gly 15 20 25TTT TTG CTA ATT GGA TTG ATN ACA TTA CTA TTG ATA CCA ATG ATG AAT 145 PheLeu Leu Ile Gly Leu Xaa Thr Leu Leu Leu Ile Pro Met Met Asn 30 35 40 CAAATG CAA GCG AGT ATA AAC CAT AAA CTA CAA ACA ATT GAT GCT TCT 193 Gln MetGln Ala Ser Ile Asn His Lys Leu Gln Thr Ile Asp Ala Ser 45 50 55 60 AAAGTA ATT TTG ACG ACT GTA TCT AAA ATT AAT AAA GAA GAA CTT AAG 241 Lys ValIle Leu Thr Thr Val Ser Lys Ile Asn Lys Glu Glu Leu Lys 65 70 75 AAG GGGGTA ACT ATA GGG AAG TAT GAT ATT AAG CAA AGT GAC CAA CAA 289 Lys Gly ValThr Ile Gly Lys Tyr Asp Ile Lys Gln Ser Asp Gln Gln 80 85 90 ATT TGT GCTATT TCA ANA AAT ACC ANT TCT TAT CAA AAG ACA TGT ATA 337 Ile Cys Ala IleSer Xaa Asn Thr Xaa Ser Tyr Gln Lys Thr Cys Ile 95 100 105 CAG TATAAATGTCAAA GCTTTTTCGC TCATTGAAAT GTTAGTAGCG ATGATGGTTA 393 Gln Tyr 110TAAGTATAAC TTTACTAATT GTTCCAGACT TAATTAGACT TAGTAAAACT TTTCTAATTG 453AAAGTAGGGA TTTAACAACT GTAGATTTCG AATTTTTCTC AAGAGATATT CTAGATGATT 513TTAAAGGAGT AGATAGAAAC GATATTGAAA TTAGGCAACA CCGTATCATT GTACATAAAG 573GTGAATAAAA ATGGC 588 110 amino acids amino acid linear protein 57 TyrPhe His Ile Gly Lys Arg Glu Glu Phe Val Met Lys Ser Tyr Lys 1 5 10 15Cys Lys Gly Ser Phe Leu Ile Asp Ser Met Ala Gly Phe Leu Leu Ile 20 25 30Gly Leu Xaa Thr Leu Leu Leu Ile Pro Met Met Asn Gln Met Gln Ala 35 40 45Ser Ile Asn His Lys Leu Gln Thr Ile Asp Ala Ser Lys Val Ile Leu 50 55 60Thr Thr Val Ser Lys Ile Asn Lys Glu Glu Leu Lys Lys Gly Val Thr 65 70 7580 Ile Gly Lys Tyr Asp Ile Lys Gln Ser Asp Gln Gln Ile Cys Ala Ile 85 9095 Ser Xaa Asn Thr Xaa Ser Tyr Gln Lys Thr Cys Ile Gln Tyr 100 105 110588 base pairs nucleic acid single linear other nucleic acid /desc =”DNA (genomic) (p8d26)“ CDS 318..587 58 ATTTATAGAA TAATATTTCC ATATTGGAAAAAGGGAAGAA TTCGTTATGA AAAGCTATAA 60 GTGTAAAGGT TCATTCTTAA TAGATAGTATGGCTGGATTT TTGCTAATTG GATTGATAAC 120 ATTACTATTG ATACCAATGA TGAATCAAATGCAAGCGAGT ATAAACCATA AACTACAAAC 180 AATTGATGCT TCTAAAGTAA TTTTGACGACTGTATCTAAA ATTAATAAAG AAGAACTTAA 240 GAAGGGGGTA ACTATAGGGA AGTATGATATTAAGCAAAGT GACCAACAAA TTTGTGCTAT 300 TTCAAAAAAT ACCAATT CTT ATC AAA AGACAT GTA TAC AGT ATA AAT GTC 350 Leu Ile Lys Arg His Val Tyr Ser Ile AsnVal 1 5 10 AAA GCT TTT TCG CTC ATT GAA ATG TTA GTA GCG ATG ATG GTT ATAAGT 398 Lys Ala Phe Ser Leu Ile Glu Met Leu Val Ala Met Met Val Ile Ser15 20 25 ATA ACT TTA CTA ATT GTT CCA GAC TTA ATT AGA CTT AGT AAA ACT TTT446 Ile Thr Leu Leu Ile Val Pro Asp Leu Ile Arg Leu Ser Lys Thr Phe 3035 40 CTA ATT GAA AGT AGG GAT TTA ACA ACT GTA GAT TTC GAA TTT TTC TCA494 Leu Ile Glu Ser Arg Asp Leu Thr Thr Val Asp Phe Glu Phe Phe Ser 4550 55 AGA GAT ATT CTA GAT GAT TTT AAA GGA GTA GAT AGA AAC GAT ATT GAA542 Arg Asp Ile Leu Asp Asp Phe Lys Gly Val Asp Arg Asn Asp Ile Glu 6065 70 75 ATT AGG CAA CAC CGT ATC ATT GTA CAT AAA GGT GNN TAA AAA TGG 587Ile Arg Gln His Arg Ile Ile Val His Lys Gly Xaa Xaa Lys Trp 80 85 90 C588 90 amino acids amino acid linear protein 59 Leu Ile Lys Arg His ValTyr Ser Ile Asn Val Lys Ala Phe Ser Leu 1 5 10 15 Ile Glu Met Leu ValAla Met Met Val Ile Ser Ile Thr Leu Leu Ile 20 25 30 Val Pro Asp Leu IleArg Leu Ser Lys Thr Phe Leu Ile Glu Ser Arg 35 40 45 Asp Leu Thr Thr ValAsp Phe Glu Phe Phe Ser Arg Asp Ile Leu Asp 50 55 60 Asp Phe Lys Gly ValAsp Arg Asn Asp Ile Glu Ile Arg Gln His Arg 65 70 75 80 Ile Ile Val HisLys Gly Xaa Xaa Lys Trp 85 90 1338 base pairs nucleic acid single linearother nucleic acid /desc = ”DNA (genomic) (p9b65)“ CDS 1..1335 60 ATGATC CGG CTT GTG ACG ATG GGG AAA AGT TCT GAG GCC GGG GTG TCT 48 Met IleArg Leu Val Thr Met Gly Lys Ser Ser Glu Ala Gly Val Ser 1 5 10 15 TCTTTC CAG GCG TTG ACG ATG TCG TTG TCC GGC CGC ATC GGC GTC GGG 96 Ser PheGln Ala Leu Thr Met Ser Leu Ser Gly Arg Ile Gly Val Gly 20 25 30 AAC GTGGCC GGG ACG GCG ACG GGG ATT GCG TAC GGG GGG CCG GGC GCG 144 Asn Val AlaGly Thr Ala Thr Gly Ile Ala Tyr Gly Gly Pro Gly Ala 35 40 45 GTC TTT TGGATG TGG GTG ATC ACC TTT ATC GGA GCG GCG ACC GCG TAT 192 Val Phe Trp MetTrp Val Ile Thr Phe Ile Gly Ala Ala Thr Ala Tyr 50 55 60 GTC GAG TCG ACGTGG CGC AAA TTT ATA AAG AGG AAC AAG ACG GAC AAT 240 Val Glu Ser Thr TrpArg Lys Phe Ile Lys Arg Asn Lys Thr Asp Asn 65 70 75 80 ACC GTG GCG GTCCGG CGT TCT ACA TTG AAA AAG GCC TTG GCT GGA AAT 288 Thr Val Ala Val ArgArg Ser Thr Leu Lys Lys Ala Leu Ala Gly Asn 85 90 95 GGT TTG CGG TGT AGTCGC GCG GCG ATC ATT CTC TCG ATG GCG GTG CTG 336 Gly Leu Arg Cys Ser ArgAla Ala Ile Ile Leu Ser Met Ala Val Leu 100 105 110 ATG CCG GGA ATT CAAGCA AAC TCG ATT GCC GAC AGC TTT TCG AAT GCG 384 Met Pro Gly Ile Gln AlaAsn Ser Ile Ala Asp Ser Phe Ser Asn Ala 115 120 125 TTT GGC ATT CCG AAATTG GTG ACG GGA ATT TTC GTG ATT GCC GTT CTT 432 Phe Gly Ile Pro Lys LeuVal Thr Gly Ile Phe Val Ile Ala Val Leu 130 135 140 GGC TTT ACG ATT TTTGGC GGA GTG AAG CGG ATC GCG AAA ACG GCG GAA 480 Gly Phe Thr Ile Phe GlyGly Val Lys Arg Ile Ala Lys Thr Ala Glu 145 150 155 160 ATT GTC GTG CCGTTT ATG GCA GTT GGC TAT TTG TTC GTC GCG ATT GCC 528 Ile Val Val Pro PheMet Ala Val Gly Tyr Leu Phe Val Ala Ile Ala 165 170 175 ATT ATT GCG GCCAAT ATT GAA AAA GTC CCG GAT GTG TTT GGT TTG ATT 576 Ile Ile Ala Ala AsnIle Glu Lys Val Pro Asp Val Phe Gly Leu Ile 180 185 190 TTC AAA AGC GCGTTT GGC GCT GAT CAA GTG TTT GGC GGC ATT CTT GGT 624 Phe Lys Ser Ala PheGly Ala Asp Gln Val Phe Gly Gly Ile Leu Gly 195 200 205 TCG GCG GTG ATGTGG GGG GTC AAA CGC GGC CTT TAT GCG AAT GAA GCG 672 Ser Ala Val Met TrpGly Val Lys Arg Gly Leu Tyr Ala Asn Glu Ala 210 215 220 GGG CAA GGG ACGGGC GCC CAC CCG GCA GCG GCG GCG GAA GTG TCC CAC 720 Gly Gln Gly Thr GlyAla His Pro Ala Ala Ala Ala Glu Val Ser His 225 230 235 240 CCG GCG AAGCAG GGG CTT GTG CAG GCA TTT TCG ATC TAT TTG GAC GTG 768 Pro Ala Lys GlnGly Leu Val Gln Ala Phe Ser Ile Tyr Leu Asp Val 245 250 255 TTC TTG GTCGTG ACG GCG ACG GCG CTG ATG ATT TTG TTT ACG GGT CAA 816 Phe Leu Val ValThr Ala Thr Ala Leu Met Ile Leu Phe Thr Gly Gln 260 265 270 TAC AAT GTGATC AAT GAA AAA ACG GGA GAG ACG ATT GTC GAG CAT TTG 864 Tyr Asn Val IleAsn Glu Lys Thr Gly Glu Thr Ile Val Glu His Leu 275 280 285 AAA GGG GTGGAA CCG GGC GCA GGG TAT ACG CAG GCG GCG GTG GAC ACG 912 Lys Gly Val GluPro Gly Ala Gly Tyr Thr Gln Ala Ala Val Asp Thr 290 295 300 CTC TTC CCGGGA TTC GGG TCG GCC TTT ATT GCG ATC GCT CTG TTC TTC 960 Leu Phe Pro GlyPhe Gly Ser Ala Phe Ile Ala Ile Ala Leu Phe Phe 305 310 315 320 TTC GCGTTT ACG ACG ATG TAC GCG TAT TAC TAT ATT GCC GAG ACG AAC 1008 Phe Ala PheThr Thr Met Tyr Ala Tyr Tyr Tyr Ile Ala Glu Thr Asn 325 330 335 CTC GCCTAT TTG GTG CGC AGT GAA AAG AGG GGA ACG GCC TTC TTT GCC 1056 Leu Ala TyrLeu Val Arg Ser Glu Lys Arg Gly Thr Ala Phe Phe Ala 340 345 350 TTG AAGCTC GTC TTT TTG GCG GCC ACG TTC TAT GGA ACG GTC AAA ACG 1104 Leu Lys LeuVal Phe Leu Ala Ala Thr Phe Tyr Gly Thr Val Lys Thr 355 360 365 GCG ACGACG GCG TGG GCG ATG GGC GAC ATC GGG CTT GGC ATC ATG GTG 1152 Ala Thr ThrAla Trp Ala Met Gly Asp Ile Gly Leu Gly Ile Met Val 370 375 380 TGG CTCAAC TTG ATT GCG ATC TTG TTG TTG TTT AAA CCG GCC TAT ATG 1200 Trp Leu AsnLeu Ile Ala Ile Leu Leu Leu Phe Lys Pro Ala Tyr Met 385 390 395 400 GCCTTG AAA GAT TAT GAA GAA CAG CTG AAG CAA GGG AAA GAT CCG GAG 1248 Ala LeuLys Asp Tyr Glu Glu Gln Leu Lys Gln Gly Lys Asp Pro Glu 405 410 415 TTCAAC GCG TCG AAA TAC GGA ATC AAG AAC GCG AAA TTC TGG GAA AAT 1296 Phe AsnAla Ser Lys Tyr Gly Ile Lys Asn Ala Lys Phe Trp Glu Asn 420 425 430 GGATAT AAG AGA TGG GAA GAA AAG AAA GGG AAG GCA TTG TAA 1338 Gly Tyr Lys ArgTrp Glu Glu Lys Lys Gly Lys Ala Leu 435 440 445 445 amino acids aminoacid linear protein 61 Met Ile Arg Leu Val Thr Met Gly Lys Ser Ser GluAla Gly Val Ser 1 5 10 15 Ser Phe Gln Ala Leu Thr Met Ser Leu Ser GlyArg Ile Gly Val Gly 20 25 30 Asn Val Ala Gly Thr Ala Thr Gly Ile Ala TyrGly Gly Pro Gly Ala 35 40 45 Val Phe Trp Met Trp Val Ile Thr Phe Ile GlyAla Ala Thr Ala Tyr 50 55 60 Val Glu Ser Thr Trp Arg Lys Phe Ile Lys ArgAsn Lys Thr Asp Asn 65 70 75 80 Thr Val Ala Val Arg Arg Ser Thr Leu LysLys Ala Leu Ala Gly Asn 85 90 95 Gly Leu Arg Cys Ser Arg Ala Ala Ile IleLeu Ser Met Ala Val Leu 100 105 110 Met Pro Gly Ile Gln Ala Asn Ser IleAla Asp Ser Phe Ser Asn Ala 115 120 125 Phe Gly Ile Pro Lys Leu Val ThrGly Ile Phe Val Ile Ala Val Leu 130 135 140 Gly Phe Thr Ile Phe Gly GlyVal Lys Arg Ile Ala Lys Thr Ala Glu 145 150 155 160 Ile Val Val Pro PheMet Ala Val Gly Tyr Leu Phe Val Ala Ile Ala 165 170 175 Ile Ile Ala AlaAsn Ile Glu Lys Val Pro Asp Val Phe Gly Leu Ile 180 185 190 Phe Lys SerAla Phe Gly Ala Asp Gln Val Phe Gly Gly Ile Leu Gly 195 200 205 Ser AlaVal Met Trp Gly Val Lys Arg Gly Leu Tyr Ala Asn Glu Ala 210 215 220 GlyGln Gly Thr Gly Ala His Pro Ala Ala Ala Ala Glu Val Ser His 225 230 235240 Pro Ala Lys Gln Gly Leu Val Gln Ala Phe Ser Ile Tyr Leu Asp Val 245250 255 Phe Leu Val Val Thr Ala Thr Ala Leu Met Ile Leu Phe Thr Gly Gln260 265 270 Tyr Asn Val Ile Asn Glu Lys Thr Gly Glu Thr Ile Val Glu HisLeu 275 280 285 Lys Gly Val Glu Pro Gly Ala Gly Tyr Thr Gln Ala Ala ValAsp Thr 290 295 300 Leu Phe Pro Gly Phe Gly Ser Ala Phe Ile Ala Ile AlaLeu Phe Phe 305 310 315 320 Phe Ala Phe Thr Thr Met Tyr Ala Tyr Tyr TyrIle Ala Glu Thr Asn 325 330 335 Leu Ala Tyr Leu Val Arg Ser Glu Lys ArgGly Thr Ala Phe Phe Ala 340 345 350 Leu Lys Leu Val Phe Leu Ala Ala ThrPhe Tyr Gly Thr Val Lys Thr 355 360 365 Ala Thr Thr Ala Trp Ala Met GlyAsp Ile Gly Leu Gly Ile Met Val 370 375 380 Trp Leu Asn Leu Ile Ala IleLeu Leu Leu Phe Lys Pro Ala Tyr Met 385 390 395 400 Ala Leu Lys Asp TyrGlu Glu Gln Leu Lys Gln Gly Lys Asp Pro Glu 405 410 415 Phe Asn Ala SerLys Tyr Gly Ile Lys Asn Ala Lys Phe Trp Glu Asn 420 425 430 Gly Tyr LysArg Trp Glu Glu Lys Lys Gly Lys Ala Leu 435 440 445 296 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p10b32)“ CDS 1..294 62 ATG CAA ATA GAA CTT ACT GAT GCA GCA GTA ACT TGGTTT AAA AAT GAA 48 Met Gln Ile Glu Leu Thr Asp Ala Ala Val Thr Trp PheLys Asn Glu 1 5 10 15 CTT GAG TTG CCT GAA AAT AAT AAA GTG CTC GTG TTTTTT GTA AGA TAT 96 Leu Glu Leu Pro Glu Asn Asn Lys Val Leu Val Phe PheVal Arg Tyr 20 25 30 GGT GGC GAA TTC CAA CTC AAG CAA GGA TTT AGT CCT GCTTTT ACA GTT 144 Gly Gly Glu Phe Gln Leu Lys Gln Gly Phe Ser Pro Ala PheThr Val 35 40 45 GAA CCA AAG GAA GAT GTT GAT ATT GGC TAT GAA CAA CAA TATGAC GAT 192 Glu Pro Lys Glu Asp Val Asp Ile Gly Tyr Glu Gln Gln Tyr AspAsp 50 55 60 TTA AAT GTT GTC GTA GCG GAA AAA GAT TTG TGG TAC TTT GAA GATGAC 240 Leu Asn Val Val Val Ala Glu Lys Asp Leu Trp Tyr Phe Glu Asp Asp65 70 75 80 CAC ATT ATT GTA AAT GTA GTT GTC ACG AAG ATG AAT TTC TTA TTCCAC 288 His Ile Ile Val Asn Val Val Val Thr Lys Met Asn Phe Leu Phe His85 90 95 CAA ATA AC 296 Gln Ile 98 amino acids amino acid linear protein63 Met Gln Ile Glu Leu Thr Asp Ala Ala Val Thr Trp Phe Lys Asn Glu 1 510 15 Leu Glu Leu Pro Glu Asn Asn Lys Val Leu Val Phe Phe Val Arg Tyr 2025 30 Gly Gly Glu Phe Gln Leu Lys Gln Gly Phe Ser Pro Ala Phe Thr Val 3540 45 Glu Pro Lys Glu Asp Val Asp Ile Gly Tyr Glu Gln Gln Tyr Asp Asp 5055 60 Leu Asn Val Val Val Ala Glu Lys Asp Leu Trp Tyr Phe Glu Asp Asp 6570 75 80 His Ile Ile Val Asn Val Val Val Thr Lys Met Asn Phe Leu Phe His85 90 95 Gln Ile 433 base pairs nucleic acid single linear other nucleicacid /desc = ”DNA(genomic) (p10b85)“ 64 GNAACACTNC TNTTTCATTT GTGAATAATTTCACAATTAT TTTATCCTTT GGTTATGAAT 60 ATGTAAATAC CATTAATTAA TTATTCATATTTTATCTATA ATTTGTTAAT AAATATTTAA 120 TATATTATNN TTATNCTTAA ATATTANGNGCAATTATACC ACTATATTTA TTTCTTATAC 180 TCCATTTGAT TTATAGTTGT ATTAATACATTGACTCAAAA ACTAATTAAT CAAATATGTT 240 TTTTAGATTN ATAAAGTTGT AACTGTACTATTTNGTAGTG TANGGTAATT TATTNGGATG 300 AAATATAATT CNNNNTACTC TATNGATTAATCAAATATGT ATCTATCAAA ATTCGGCTTA 360 TTTATNCACT CTGACATATC CANATACGCAAAAAGACTAT NNCTACCTTG TATCGANAGA 420 AATAGTCTTT TTA 433 208 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p10b89)“ CDS 1..207 65 TAC TAT AAA AGC ACC CAC TCA GTC ACT AGT TTG GGCAGT TAT TGT ATG 48 Tyr Tyr Lys Ser Thr His Ser Val Thr Ser Leu Gly SerTyr Cys Met 1 5 10 15 CCT ATT GAA CTC AAT GCG TAT ATT ACA ATA CCT TTTTCG CAT ATT CAT 96 Pro Ile Glu Leu Asn Ala Tyr Ile Thr Ile Pro Phe SerHis Ile His 20 25 30 ATA AGA TCT TTG CAT TCN TTA AGC TTA AAT TTT CTA TTCTTC NTT CTC 144 Ile Arg Ser Leu His Ser Leu Ser Leu Asn Phe Leu Phe PheXaa Leu 35 40 45 TAC GGC GGC ATA GCA TTA ATA TTA CCG GAA CTA ATC CCA GTANCN GTA 192 Tyr Gly Gly Ile Ala Leu Ile Leu Pro Glu Leu Ile Pro Val XaaVal 50 55 60 TTA ATT GGA TAC CCG G 208 Leu Ile Gly Tyr Pro 65 69 aminoacids amino acid linear protein 66 Tyr Tyr Lys Ser Thr His Ser Val ThrSer Leu Gly Ser Tyr Cys Met 1 5 10 15 Pro Ile Glu Leu Asn Ala Tyr IleThr Ile Pro Phe Ser His Ile His 20 25 30 Ile Arg Ser Leu His Ser Leu SerLeu Asn Phe Leu Phe Phe Xaa Leu 35 40 45 Tyr Gly Gly Ile Ala Leu Ile LeuPro Glu Leu Ile Pro Val Xaa Val 50 55 60 Leu Ile Gly Tyr Pro 65 1041base pairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p10c30)“ CDS 1..1038 67 AGA AGT CAA ATC ATT ANT GGC GTC NTATCG AGT ATA CTA TTA ACT TCA 48 Arg Ser Gln Ile Ile Xaa Gly Val Xaa SerSer Ile Leu Leu Thr Ser 1 5 10 15 ACT ATT TTA GCA ATT GCA TAT ATT TTAATG TGG TTT AAC GGC CAT ATG 96 Thr Ile Leu Ala Ile Ala Tyr Ile Leu MetTrp Phe Asn Gly His Met 20 25 30 ACA CTA ACT TTG ACC TTA ACG ACA ATA ATTACA AGC TGT TTA ACC TTA 144 Thr Leu Thr Leu Thr Leu Thr Thr Ile Ile ThrSer Cys Leu Thr Leu 35 40 45 TTA ATA TGT AGT ATT TTT ATT AAT CCA CTT ATACAA AAA ATT AAG CAG 192 Leu Ile Cys Ser Ile Phe Ile Asn Pro Leu Ile GlnLys Ile Lys Gln 50 55 60 TTT AAT ATA AAA ACT AAG CAA TTT GCT AAC GGA AATTAC GCA AGC AAT 240 Phe Asn Ile Lys Thr Lys Gln Phe Ala Asn Gly Asn TyrAla Ser Asn 65 70 75 80 GAT AAA ACG TTT AAT TCA CCA AAA GAA ATT TAT GAATTA AAT CAA TCT 288 Asp Lys Thr Phe Asn Ser Pro Lys Glu Ile Tyr Glu LeuAsn Gln Ser 85 90 95 TTT AAT AAA ATG GCT TCT GAA ATT ACG CAA CAA ATG AATCAA ATT AAA 336 Phe Asn Lys Met Ala Ser Glu Ile Thr Gln Gln Met Asn GlnIle Lys 100 105 110 TCC GAA CAA CAA GAA AAA ACA GAA CTG ATT CAA AAC TTAGCC CAT GAT 384 Ser Glu Gln Gln Glu Lys Thr Glu Leu Ile Gln Asn Leu AlaHis Asp 115 120 125 TTA AAA ACA CCT TTA GCA AGC ATT ATT TCA TAT TCT GAAGGA CTA CGT 432 Leu Lys Thr Pro Leu Ala Ser Ile Ile Ser Tyr Ser Glu GlyLeu Arg 130 135 140 GAT GGT ATA ATC ACT AAG GAT CAT GAG ATT AAA GAG TCATAC GAC ATA 480 Asp Gly Ile Ile Thr Lys Asp His Glu Ile Lys Glu Ser TyrAsp Ile 145 150 155 160 TTA ATT AAA CAA GCA AAC AGA TTA TCA ACA TTA TTTGAT GAT ATG ACT 528 Leu Ile Lys Gln Ala Asn Arg Leu Ser Thr Leu Phe AspAsp Met Thr 165 170 175 CAT ATT ATC ACT TTA AAT ACA GGT AAA ACA TAT CCCCCA GAA TTA ATA 576 His Ile Ile Thr Leu Asn Thr Gly Lys Thr Tyr Pro ProGlu Leu Ile 180 185 190 CAA CTA GAC CAA TTA CTT GTA TCA ATA TTG CAA CCATAT GAG CAA CGT 624 Gln Leu Asp Gln Leu Leu Val Ser Ile Leu Gln Pro TyrGlu Gln Arg 195 200 205 ATC AAA CAT GAA AAC CGC ACA TTA GAA GTG AAT TTCTGT AAC GAA ATT 672 Ile Lys His Glu Asn Arg Thr Leu Glu Val Asn Phe CysAsn Glu Ile 210 215 220 GAT GCA TTT TAT CAA TAT CGA ACG CCA CTT GAG CGTATT TTA ACA AAC 720 Asp Ala Phe Tyr Gln Tyr Arg Thr Pro Leu Glu Arg IleLeu Thr Asn 225 230 235 240 TTA CTT GAT AAT GCG CTA AAA TTT TCA AAT GTTGGT AGT CGC ATT GAT 768 Leu Leu Asp Asn Ala Leu Lys Phe Ser Asn Val GlySer Arg Ile Asp 245 250 255 ATT AAT ATT AGT GAA AAC GAA GAT CAA GAT ACTATC GAC ATT GCT ATT 816 Ile Asn Ile Ser Glu Asn Glu Asp Gln Asp Thr IleAsp Ile Ala Ile 260 265 270 AGC GAT GAA GGT ATT GGC ATT ATA CCA GAA CTACAA GAA CGT ATA TTC 864 Ser Asp Glu Gly Ile Gly Ile Ile Pro Glu Leu GlnGlu Arg Ile Phe 275 280 285 GAA CGT ACA TTC AGA GTA GAA AAC TCT CGT AATACA AAA ACG GGT GGT 912 Glu Arg Thr Phe Arg Val Glu Asn Ser Arg Asn ThrLys Thr Gly Gly 290 295 300 TCT GGA TTA GGC TTA TAT ATA GCT AAT GAA CTCGCG CAA CAA AAT AAC 960 Ser Gly Leu Gly Leu Tyr Ile Ala Asn Glu Leu AlaGln Gln Asn Asn 305 310 315 320 GCA AAA ATC AGT GTA AGC AGT GAT ATA GATGTA GGA ACT ACG ATG ACT 1008 Ala Lys Ile Ser Val Ser Ser Asp Ile Asp ValGly Thr Thr Met Thr 325 330 335 GTA ACA TTA CAC AAA TTA GAC ATT ACG TCATAA 1041 Val Thr Leu His Lys Leu Asp Ile Thr Ser 340 345 346 amino acidsamino acid linear protein 68 Arg Ser Gln Ile Ile Xaa Gly Val Xaa Ser SerIle Leu Leu Thr Ser 1 5 10 15 Thr Ile Leu Ala Ile Ala Tyr Ile Leu MetTrp Phe Asn Gly His Met 20 25 30 Thr Leu Thr Leu Thr Leu Thr Thr Ile IleThr Ser Cys Leu Thr Leu 35 40 45 Leu Ile Cys Ser Ile Phe Ile Asn Pro LeuIle Gln Lys Ile Lys Gln 50 55 60 Phe Asn Ile Lys Thr Lys Gln Phe Ala AsnGly Asn Tyr Ala Ser Asn 65 70 75 80 Asp Lys Thr Phe Asn Ser Pro Lys GluIle Tyr Glu Leu Asn Gln Ser 85 90 95 Phe Asn Lys Met Ala Ser Glu Ile ThrGln Gln Met Asn Gln Ile Lys 100 105 110 Ser Glu Gln Gln Glu Lys Thr GluLeu Ile Gln Asn Leu Ala His Asp 115 120 125 Leu Lys Thr Pro Leu Ala SerIle Ile Ser Tyr Ser Glu Gly Leu Arg 130 135 140 Asp Gly Ile Ile Thr LysAsp His Glu Ile Lys Glu Ser Tyr Asp Ile 145 150 155 160 Leu Ile Lys GlnAla Asn Arg Leu Ser Thr Leu Phe Asp Asp Met Thr 165 170 175 His Ile IleThr Leu Asn Thr Gly Lys Thr Tyr Pro Pro Glu Leu Ile 180 185 190 Gln LeuAsp Gln Leu Leu Val Ser Ile Leu Gln Pro Tyr Glu Gln Arg 195 200 205 IleLys His Glu Asn Arg Thr Leu Glu Val Asn Phe Cys Asn Glu Ile 210 215 220Asp Ala Phe Tyr Gln Tyr Arg Thr Pro Leu Glu Arg Ile Leu Thr Asn 225 230235 240 Leu Leu Asp Asn Ala Leu Lys Phe Ser Asn Val Gly Ser Arg Ile Asp245 250 255 Ile Asn Ile Ser Glu Asn Glu Asp Gln Asp Thr Ile Asp Ile AlaIle 260 265 270 Ser Asp Glu Gly Ile Gly Ile Ile Pro Glu Leu Gln Glu ArgIle Phe 275 280 285 Glu Arg Thr Phe Arg Val Glu Asn Ser Arg Asn Thr LysThr Gly Gly 290 295 300 Ser Gly Leu Gly Leu Tyr Ile Ala Asn Glu Leu AlaGln Gln Asn Asn 305 310 315 320 Ala Lys Ile Ser Val Ser Ser Asp Ile AspVal Gly Thr Thr Met Thr 325 330 335 Val Thr Leu His Lys Leu Asp Ile ThrSer 340 345 392 base pairs nucleic acid single linear other nucleic acid/desc = ”DNA (genomic) (p10c52)“ CDS 1..390 69 GTG AAC TAT GAA TAT TTCGAA TAT TCG GTT AAT TTA GGT GGT ACC ACG 48 Val Asn Tyr Glu Tyr Phe GluTyr Ser Val Asn Leu Gly Gly Thr Thr 1 5 10 15 YGT CAC GTC CTT TAT ATTGAT AAG GAT GCT GGC GCT TTT TTG AAA GGA 96 Xaa His Val Leu Tyr Ile AspLys Asp Ala Gly Ala Phe Leu Lys Gly 20 25 30 GCG TAT AGA ATG GAT ATA TTTTAT AAA AAA ATA AAA GCA AAT GTA ACG 144 Ala Tyr Arg Met Asp Ile Phe TyrLys Lys Ile Lys Ala Asn Val Thr 35 40 45 CCC GAA GTT TTA GCA CAA CTT CATTCC AAG AAG ATC ATT TTG GAA AGT 192 Pro Glu Val Leu Ala Gln Leu His SerLys Lys Ile Ile Leu Glu Ser 50 55 60 ACA AAT CAA CAA CAA ACT AAA GGT CGCTAT TCA GTT GTT ATT TTT GAT 240 Thr Asn Gln Gln Gln Thr Lys Gly Arg TyrSer Val Val Ile Phe Asp 65 70 75 80 ATT TAT GGC ACT TTA ACT TTA GAT AATGAT GTA TTA TCA GTA AGT ACT 288 Ile Tyr Gly Thr Leu Thr Leu Asp Asn AspVal Leu Ser Val Ser Thr 85 90 95 TTA AAA GAA TCG TAT CAA ATC ACT GAA AGACCG TAC CAT TAT TTA ACG 336 Leu Lys Glu Ser Tyr Gln Ile Thr Glu Arg ProTyr His Tyr Leu Thr 100 105 110 ACT AAN ATA AAT GAA GAC TAC CAT AAT ATTCCA AGA TGA GGC AAC TTA 384 Thr Xaa Ile Asn Glu Asp Tyr His Asn Ile ProArg Xaa Gly Asn Leu 115 120 125 AGT CAT TA 392 Ser His 130 130 aminoacids amino acid linear protein 70 Val Asn Tyr Glu Tyr Phe Glu Tyr SerVal Asn Leu Gly Gly Thr Thr 1 5 10 15 Xaa His Val Leu Tyr Ile Asp LysAsp Ala Gly Ala Phe Leu Lys Gly 20 25 30 Ala Tyr Arg Met Asp Ile Phe TyrLys Lys Ile Lys Ala Asn Val Thr 35 40 45 Pro Glu Val Leu Ala Gln Leu HisSer Lys Lys Ile Ile Leu Glu Ser 50 55 60 Thr Asn Gln Gln Gln Thr Lys GlyArg Tyr Ser Val Val Ile Phe Asp 65 70 75 80 Ile Tyr Gly Thr Leu Thr LeuAsp Asn Asp Val Leu Ser Val Ser Thr 85 90 95 Leu Lys Glu Ser Tyr Gln IleThr Glu Arg Pro Tyr His Tyr Leu Thr 100 105 110 Thr Xaa Ile Asn Glu AspTyr His Asn Ile Pro Arg Xaa Gly Asn Leu 115 120 125 Ser His 130 1071base pairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p10d9)“ CDS 1..1068 71 ATG AAA ATG AGA ACA ATT GCT AAA ACCAGT TTA GCA CTA GGG CTT TTA 48 Met Lys Met Arg Thr Ile Ala Lys Thr SerLeu Ala Leu Gly Leu Leu 1 5 10 15 ACA ACA GGC GCA ATT ACA GTA ACG ACGCAA TCG GTC AAA GCA GAA AAA 96 Thr Thr Gly Ala Ile Thr Val Thr Thr GlnSer Val Lys Ala Glu Lys 20 25 30 ATA CAA TCA ACT AAA GTT GAC AAA GTA CCAACG CTT AAA GCA GAG CGA 144 Ile Gln Ser Thr Lys Val Asp Lys Val Pro ThrLeu Lys Ala Glu Arg 35 40 45 TTA GCA ATG ATA AAC ATA ACA GCA GGT GCA AATTCA GCG ACA ACA CAA 192 Leu Ala Met Ile Asn Ile Thr Ala Gly Ala Asn SerAla Thr Thr Gln 50 55 60 GCA GCT AAC ACA AGA CAA GAA CGC ACG CCT AAA CTCGAA AAG GCA CCA 240 Ala Ala Asn Thr Arg Gln Glu Arg Thr Pro Lys Leu GluLys Ala Pro 65 70 75 80 AAT ACT AAT GAG GAA AAA ACC TCA GCT TCC AAA ATAGAA AAA ATA TCA 288 Asn Thr Asn Glu Glu Lys Thr Ser Ala Ser Lys Ile GluLys Ile Ser 85 90 95 CAA CCT AAA CAA GAA GAG CAG AAA ACG CTT AAT ATA TCAGCA ACG CCA 336 Gln Pro Lys Gln Glu Glu Gln Lys Thr Leu Asn Ile Ser AlaThr Pro 100 105 110 GCG CCT AAA CAA GAA CAA TCA CAA ACG ACA ACC GAA TCCACA ACG CCG 384 Ala Pro Lys Gln Glu Gln Ser Gln Thr Thr Thr Glu Ser ThrThr Pro 115 120 125 AAA ACT AAA GTG ACA ACA CCT CCA TCA ACA AAC ACG CCACAA CCA ATG 432 Lys Thr Lys Val Thr Thr Pro Pro Ser Thr Asn Thr Pro GlnPro Met 130 135 140 CAA TCT ACT AAA TCA GAC ACA CCA CAA TCT CCA ACC ATAAAA CAA GCA 480 Gln Ser Thr Lys Ser Asp Thr Pro Gln Ser Pro Thr Ile LysGln Ala 145 150 155 160 CAA ACA GAT ATG ACT CCT AAA TAT GAA GAT TTA AGAGCG TAT TAT ACA 528 Gln Thr Asp Met Thr Pro Lys Tyr Glu Asp Leu Arg AlaTyr Tyr Thr 165 170 175 AAA CCG AGT TTT GAA TTT GAA AAG CAG TTT GGA TTTATG CTC AAA CCA 576 Lys Pro Ser Phe Glu Phe Glu Lys Gln Phe Gly Phe MetLeu Lys Pro 180 185 190 TGG ACG ACG GTT AGG TTT ATG AAT GTT ATT CCA AATAGG TTC ATC TAT 624 Trp Thr Thr Val Arg Phe Met Asn Val Ile Pro Asn ArgPhe Ile Tyr 195 200 205 AAA ATA GCT TTA GTT GGA AAA GAT GAG AAA AAA TATAAA GAT GGA CCT 672 Lys Ile Ala Leu Val Gly Lys Asp Glu Lys Lys Tyr LysAsp Gly Pro 210 215 220 TAC GAT AAT ATC GAT GTA TTT ATC GTT TTA GAA GACAAT AAA TAT CAA 720 Tyr Asp Asn Ile Asp Val Phe Ile Val Leu Glu Asp AsnLys Tyr Gln 225 230 235 240 TTG AAA AAA TAT TCT GTC GGT GGC ATC ACG AAGACT AAT AGT AAA AAA 768 Leu Lys Lys Tyr Ser Val Gly Gly Ile Thr Lys ThrAsn Ser Lys Lys 245 250 255 GTT AAT CAC AAA GTA GAA TTA AGC ATT ACT AAAAAA GAT AAT CAA GGT 816 Val Asn His Lys Val Glu Leu Ser Ile Thr Lys LysAsp Asn Gln Gly 260 265 270 ATG ATT TCA CGC GAT GTT TCA GAA TAC ATG ATTACT AAG GAA GAG ATT 864 Met Ile Ser Arg Asp Val Ser Glu Tyr Met Ile ThrLys Glu Glu Ile 275 280 285 TCC TTG AAA GAG CTT GAT TTT AAA TTG AGA AAACAA CTT ATT GAA AAA 912 Ser Leu Lys Glu Leu Asp Phe Lys Leu Arg Lys GlnLeu Ile Glu Lys 290 295 300 CAT AAT CTT TAC GGT AAC ATG GGT TCA GGA ACAATC GTT ATT AAA ATG 960 His Asn Leu Tyr Gly Asn Met Gly Ser Gly Thr IleVal Ile Lys Met 305 310 315 320 AAA AAC GGT GGG AAA TAT ACG TTT GAA TTACAC AAA AAA CTG CAA GAG 1008 Lys Asn Gly Gly Lys Tyr Thr Phe Glu Leu HisLys Lys Leu Gln Glu 325 330 335 CAT CGT ATG GCA GAC GTC ATA GAT GGC ACTAAT ATT GAT AAC ATT GAA 1056 His Arg Met Ala Asp Val Ile Asp Gly Thr AsnIle Asp Asn Ile Glu 340 345 350 GTG AAT ATA AAA TAA 1071 Val Asn Ile Lys355 356 amino acids amino acid linear protein 72 Met Lys Met Arg Thr IleAla Lys Thr Ser Leu Ala Leu Gly Leu Leu 1 5 10 15 Thr Thr Gly Ala IleThr Val Thr Thr Gln Ser Val Lys Ala Glu Lys 20 25 30 Ile Gln Ser Thr LysVal Asp Lys Val Pro Thr Leu Lys Ala Glu Arg 35 40 45 Leu Ala Met Ile AsnIle Thr Ala Gly Ala Asn Ser Ala Thr Thr Gln 50 55 60 Ala Ala Asn Thr ArgGln Glu Arg Thr Pro Lys Leu Glu Lys Ala Pro 65 70 75 80 Asn Thr Asn GluGlu Lys Thr Ser Ala Ser Lys Ile Glu Lys Ile Ser 85 90 95 Gln Pro Lys GlnGlu Glu Gln Lys Thr Leu Asn Ile Ser Ala Thr Pro 100 105 110 Ala Pro LysGln Glu Gln Ser Gln Thr Thr Thr Glu Ser Thr Thr Pro 115 120 125 Lys ThrLys Val Thr Thr Pro Pro Ser Thr Asn Thr Pro Gln Pro Met 130 135 140 GlnSer Thr Lys Ser Asp Thr Pro Gln Ser Pro Thr Ile Lys Gln Ala 145 150 155160 Gln Thr Asp Met Thr Pro Lys Tyr Glu Asp Leu Arg Ala Tyr Tyr Thr 165170 175 Lys Pro Ser Phe Glu Phe Glu Lys Gln Phe Gly Phe Met Leu Lys Pro180 185 190 Trp Thr Thr Val Arg Phe Met Asn Val Ile Pro Asn Arg Phe IleTyr 195 200 205 Lys Ile Ala Leu Val Gly Lys Asp Glu Lys Lys Tyr Lys AspGly Pro 210 215 220 Tyr Asp Asn Ile Asp Val Phe Ile Val Leu Glu Asp AsnLys Tyr Gln 225 230 235 240 Leu Lys Lys Tyr Ser Val Gly Gly Ile Thr LysThr Asn Ser Lys Lys 245 250 255 Val Asn His Lys Val Glu Leu Ser Ile ThrLys Lys Asp Asn Gln Gly 260 265 270 Met Ile Ser Arg Asp Val Ser Glu TyrMet Ile Thr Lys Glu Glu Ile 275 280 285 Ser Leu Lys Glu Leu Asp Phe LysLeu Arg Lys Gln Leu Ile Glu Lys 290 295 300 His Asn Leu Tyr Gly Asn MetGly Ser Gly Thr Ile Val Ile Lys Met 305 310 315 320 Lys Asn Gly Gly LysTyr Thr Phe Glu Leu His Lys Lys Leu Gln Glu 325 330 335 His Arg Met AlaAsp Val Ile Asp Gly Thr Asn Ile Asp Asn Ile Glu 340 345 350 Val Asn IleLys 355 1590 base pairs nucleic acid single linear other nucleic acid/desc = ”DNA (genomic) (p11c12)“ CDS 1..1587 73 AGA GCA AAC CCT AGA AAAGGA GGT GTT TGT GTG AAT TTA TTA AGC CTC 48 Arg Ala Asn Pro Arg Lys GlyGly Val Cys Val Asn Leu Leu Ser Leu 1 5 10 15 CTA CTC ATT TTG CTG GGGATC ATT CTA GGA GTT GTT GGA GGG TAT GTT 96 Leu Leu Ile Leu Leu Gly IleIle Leu Gly Val Val Gly Gly Tyr Val 20 25 30 GTT GCC CGA AAT TTG TTG CTTCAA AAG CAA TCA CAA GCT AGA CAA ACT 144 Val Ala Arg Asn Leu Leu Leu GlnLys Gln Ser Gln Ala Arg Gln Thr 35 40 45 GCC GAA GAT ATT GTA AAT CAA GCACAT AAA GAA GCT GAC AAT ATC AAA 192 Ala Glu Asp Ile Val Asn Gln Ala HisLys Glu Ala Asp Asn Ile Lys 50 55 60 AAA GAG AAA TTA CTT GAG GCA AAA GAAGAA AAC CAA ATC CTA AGA GAA 240 Lys Glu Lys Leu Leu Glu Ala Lys Glu GluAsn Gln Ile Leu Arg Glu 65 70 75 80 CAA ACT GAA GCA GAA CTA CGA GAA AGACGT AGC GAA CTT CAA AGA CAA 288 Gln Thr Glu Ala Glu Leu Arg Glu Arg ArgSer Glu Leu Gln Arg Gln 85 90 95 GAA ACC CGA CTT CTT CAA AAA GAA GAA AACTTA GAG CGC AAA TCT GAT 336 Glu Thr Arg Leu Leu Gln Lys Glu Glu Asn LeuGlu Arg Lys Ser Asp 100 105 110 CTA TTA GAT AAA AAA GAT GAG ATT TTA GAGCAA AAA GAA TCA AAA ATT 384 Leu Leu Asp Lys Lys Asp Glu Ile Leu Glu GlnLys Glu Ser Lys Ile 115 120 125 GAA GAA AAA CAA CAA CAA GTA GAT GCA AAAGAG AGT AGT GTT CAA ACG 432 Glu Glu Lys Gln Gln Gln Val Asp Ala Lys GluSer Ser Val Gln Thr 130 135 140 TTA ATA ATG AAG CAT GAA CAA GAA TTA GAACGC ATC TCC GGT CTC ACT 480 Leu Ile Met Lys His Glu Gln Glu Leu Glu ArgIle Ser Gly Leu Thr 145 150 155 160 CAA GAA GAA GCT ATT AAT GAG CAA CTTCAA AGA GTA GAG GAA GAA CTG 528 Gln Glu Glu Ala Ile Asn Glu Gln Leu GlnArg Val Glu Glu Glu Leu 165 170 175 TCA CAA GAT ATT GCA GTA CTT GTT AAAGAA AAA GAA AAA GAA GCT AAA 576 Ser Gln Asp Ile Ala Val Leu Val Lys GluLys Glu Lys Glu Ala Lys 180 185 190 GAA AAA GTT GAT AAA ACA GCA AAA GAATTA TTA GCT ACA GCA GTA CAA 624 Glu Lys Val Asp Lys Thr Ala Lys Glu LeuLeu Ala Thr Ala Val Gln 195 200 205 AGA TTA GCA GCA GAT CAC ACA AGT GAATCA ACG GTA TCA GTA GTT AAC 672 Arg Leu Ala Ala Asp His Thr Ser Glu SerThr Val Ser Val Val Asn 210 215 220 TTA CCT AAT GAT GAG ATG AAA GGT CGAATC ATT GGA CGA GAA GGA CGA 720 Leu Pro Asn Asp Glu Met Lys Gly Arg IleIle Gly Arg Glu Gly Arg 225 230 235 240 AAC ATC CGC ACA CTT GAA ACT TTAACT GGC ATT GAT TTA ATT ATT GAT 768 Asn Ile Arg Thr Leu Glu Thr Leu ThrGly Ile Asp Leu Ile Ile Asp 245 250 255 GAC ACA CCA GAA GCG GTT ATA TTATCT GGT TTT GAT CCA ATA AGA AGA 816 Asp Thr Pro Glu Ala Val Ile Leu SerGly Phe Asp Pro Ile Arg Arg 260 265 270 GAA ATT GCT AGA ACA GCA CTT GTTAAC TTA GTA TCT GAT GGA CGT ATT 864 Glu Ile Ala Arg Thr Ala Leu Val AsnLeu Val Ser Asp Gly Arg Ile 275 280 285 CAT CCA GGT AGA ATT GAA GAT ATGGTC GAA AAA GCT AGA AAA GAA GTA 912 His Pro Gly Arg Ile Glu Asp Met ValGlu Lys Ala Arg Lys Glu Val 290 295 300 GAC GAT ATT ATT AGA GAA GCA GGTGAA CAA GCT ACA TTT GAA GTG AAC 960 Asp Asp Ile Ile Arg Glu Ala Gly GluGln Ala Thr Phe Glu Val Asn 305 310 315 320 GCA CAT AAT ATG CAT CCT GACTTA GTA AAA ATT GTA GGG CGT TTA AAC 1008 Ala His Asn Met His Pro Asp LeuVal Lys Ile Val Gly Arg Leu Asn 325 330 335 TAT CGT ACG AGT TAC GGT CAAAAT GTA CTT AAA CAT TCA ATT GAA GTT 1056 Tyr Arg Thr Ser Tyr Gly Gln AsnVal Leu Lys His Ser Ile Glu Val 340 345 350 GCG CAT CTT GCT AGT ATG TTAGCT GCT GAG CTA GGC GAA GAT GAG ACA 1104 Ala His Leu Ala Ser Met Leu AlaAla Glu Leu Gly Glu Asp Glu Thr 355 360 365 TTA GCG AAA CGA GCT GGA CTTTTA CAT GAT GTT GGT AAA GCA ATT GAT 1152 Leu Ala Lys Arg Ala Gly Leu LeuHis Asp Val Gly Lys Ala Ile Asp 370 375 380 CAT GAA GTA GAA GGT AGT CATGTT GAA ATC GGT GTA GAA TTA GCG AAA 1200 His Glu Val Glu Gly Ser His ValGlu Ile Gly Val Glu Leu Ala Lys 385 390 395 400 AAA TAT GGT GAA AAT GAAACA GTT ATT AAT GCA ATC CAT TCT CAT CAT 1248 Lys Tyr Gly Glu Asn Glu ThrVal Ile Asn Ala Ile His Ser His His 405 410 415 GGT GAT GTT GAA CCT ACATCT ATT ATA TCT ATC CTT GTT GCT GCT GCA 1296 Gly Asp Val Glu Pro Thr SerIle Ile Ser Ile Leu Val Ala Ala Ala 420 425 430 GAT GCA TTG TCT GCG GCTCGT CCA GGT GCA AGA AAA GAA ACA TTA GAG 1344 Asp Ala Leu Ser Ala Ala ArgPro Gly Ala Arg Lys Glu Thr Leu Glu 435 440 445 AAT TAT ATT CGT CGA TTAGAA CGT TTA GAA ACG TTA TCA GAA AGT TAT 1392 Asn Tyr Ile Arg Arg Leu GluArg Leu Glu Thr Leu Ser Glu Ser Tyr 450 455 460 GAT GGT GTA GAA AAA GCATTT GCG ATT CAG GCA GGT AGA GAA ATC CGA 1440 Asp Gly Val Glu Lys Ala PheAla Ile Gln Ala Gly Arg Glu Ile Arg 465 470 475 480 GTG ATT GTA TCT CCTGAA GAA ATT GAT GAT TTA AAA TCT TAT CGA TTG 1488 Val Ile Val Ser Pro GluGlu Ile Asp Asp Leu Lys Ser Tyr Arg Leu 485 490 495 GCT AGA GAT ATT AAAAAT CAG ATT GAA GAT GAA TTA CAA TAT CCT GGT 1536 Ala Arg Asp Ile Lys AsnGln Ile Glu Asp Glu Leu Gln Tyr Pro Gly 500 505 510 CAT ATC AAG GTG ACAGTT GTT CGA GAG ACT AGA GCA GTA GAA TAT GCG 1584 His Ile Lys Val Thr ValVal Arg Glu Thr Arg Ala Val Glu Tyr Ala 515 520 525 AAA TAA 1590 Lys 529amino acids amino acid linear protein 74 Arg Ala Asn Pro Arg Lys Gly GlyVal Cys Val Asn Leu Leu Ser Leu 1 5 10 15 Leu Leu Ile Leu Leu Gly IleIle Leu Gly Val Val Gly Gly Tyr Val 20 25 30 Val Ala Arg Asn Leu Leu LeuGln Lys Gln Ser Gln Ala Arg Gln Thr 35 40 45 Ala Glu Asp Ile Val Asn GlnAla His Lys Glu Ala Asp Asn Ile Lys 50 55 60 Lys Glu Lys Leu Leu Glu AlaLys Glu Glu Asn Gln Ile Leu Arg Glu 65 70 75 80 Gln Thr Glu Ala Glu LeuArg Glu Arg Arg Ser Glu Leu Gln Arg Gln 85 90 95 Glu Thr Arg Leu Leu GlnLys Glu Glu Asn Leu Glu Arg Lys Ser Asp 100 105 110 Leu Leu Asp Lys LysAsp Glu Ile Leu Glu Gln Lys Glu Ser Lys Ile 115 120 125 Glu Glu Lys GlnGln Gln Val Asp Ala Lys Glu Ser Ser Val Gln Thr 130 135 140 Leu Ile MetLys His Glu Gln Glu Leu Glu Arg Ile Ser Gly Leu Thr 145 150 155 160 GlnGlu Glu Ala Ile Asn Glu Gln Leu Gln Arg Val Glu Glu Glu Leu 165 170 175Ser Gln Asp Ile Ala Val Leu Val Lys Glu Lys Glu Lys Glu Ala Lys 180 185190 Glu Lys Val Asp Lys Thr Ala Lys Glu Leu Leu Ala Thr Ala Val Gln 195200 205 Arg Leu Ala Ala Asp His Thr Ser Glu Ser Thr Val Ser Val Val Asn210 215 220 Leu Pro Asn Asp Glu Met Lys Gly Arg Ile Ile Gly Arg Glu GlyArg 225 230 235 240 Asn Ile Arg Thr Leu Glu Thr Leu Thr Gly Ile Asp LeuIle Ile Asp 245 250 255 Asp Thr Pro Glu Ala Val Ile Leu Ser Gly Phe AspPro Ile Arg Arg 260 265 270 Glu Ile Ala Arg Thr Ala Leu Val Asn Leu ValSer Asp Gly Arg Ile 275 280 285 His Pro Gly Arg Ile Glu Asp Met Val GluLys Ala Arg Lys Glu Val 290 295 300 Asp Asp Ile Ile Arg Glu Ala Gly GluGln Ala Thr Phe Glu Val Asn 305 310 315 320 Ala His Asn Met His Pro AspLeu Val Lys Ile Val Gly Arg Leu Asn 325 330 335 Tyr Arg Thr Ser Tyr GlyGln Asn Val Leu Lys His Ser Ile Glu Val 340 345 350 Ala His Leu Ala SerMet Leu Ala Ala Glu Leu Gly Glu Asp Glu Thr 355 360 365 Leu Ala Lys ArgAla Gly Leu Leu His Asp Val Gly Lys Ala Ile Asp 370 375 380 His Glu ValGlu Gly Ser His Val Glu Ile Gly Val Glu Leu Ala Lys 385 390 395 400 LysTyr Gly Glu Asn Glu Thr Val Ile Asn Ala Ile His Ser His His 405 410 415Gly Asp Val Glu Pro Thr Ser Ile Ile Ser Ile Leu Val Ala Ala Ala 420 425430 Asp Ala Leu Ser Ala Ala Arg Pro Gly Ala Arg Lys Glu Thr Leu Glu 435440 445 Asn Tyr Ile Arg Arg Leu Glu Arg Leu Glu Thr Leu Ser Glu Ser Tyr450 455 460 Asp Gly Val Glu Lys Ala Phe Ala Ile Gln Ala Gly Arg Glu IleArg 465 470 475 480 Val Ile Val Ser Pro Glu Glu Ile Asp Asp Leu Lys SerTyr Arg Leu 485 490 495 Ala Arg Asp Ile Lys Asn Gln Ile Glu Asp Glu LeuGln Tyr Pro Gly 500 505 510 His Ile Lys Val Thr Val Val Arg Glu Thr ArgAla Val Glu Tyr Ala 515 520 525 Lys 799 base pairs nucleic acid singlelinear other nucleic acid /desc = ”DNA (genomic) (p11c66)“ CDS 1..384 75TCA TGG TAT TAT ATT GTT TGG AGT GAT TTG ATG AGA TTT GTC TTT GAT 48 SerTrp Tyr Tyr Ile Val Trp Ser Asp Leu Met Arg Phe Val Phe Asp 1 5 10 15ATT GAT GGT ACG CTT TGT TTT GAC GGC CGA TTA ATT GAC CAG ACT ATT 96 IleAsp Gly Thr Leu Cys Phe Asp Gly Arg Leu Ile Asp Gln Thr Ile 20 25 30 ATTGAT ACA TTG TTA CAA TTA CAA CAT GAT GGT CAT GAA CTT ATA TTT 144 Ile AspThr Leu Leu Gln Leu Gln His Asp Gly His Glu Leu Ile Phe 35 40 45 GCA TCAGCA CGT CCG ATT CGT GAT TTG TTG CCA GTT TTA CCA TCA GTA 192 Ala Ser AlaArg Pro Ile Arg Asp Leu Leu Pro Val Leu Pro Ser Val 50 55 60 TTT CAT CAGCAC ACA TTA ATT GGC GCA AAT GGT GCT ATG ATT TCA CAG 240 Phe His Gln HisThr Leu Ile Gly Ala Asn Gly Ala Met Ile Ser Gln 65 70 75 80 CAA TCA AAGATT TCT GTT ATC AAA CCA ATT CAT ACT GAT ACA TAT CAT 288 Gln Ser Lys IleSer Val Ile Lys Pro Ile His Thr Asp Thr Tyr His 85 90 95 CAT ATC TTC AAAATA ATT CAA AAG TAT GAG TTA GAT TAT ATT ATT GAT 336 His Ile Phe Lys IleIle Gln Lys Tyr Glu Leu Asp Tyr Ile Ile Asp 100 105 110 GAT GAT TGG AATTAT GCT GCA CAA CTT GAC GCT GNA GAA CGC GAT TTT 384 Asp Asp Trp Asn TyrAla Ala Gln Leu Asp Ala Xaa Glu Arg Asp Phe 115 120 125 TGAGCGTTTAGATCCACATA AGCTGGCCAG TTGTATTGAT GTTGCAAATA TCGACACGCC 444 AATCAAGAKTATTTTATTAA ATATAGACCC GGCACAAATT ACAACTATAT TAGACGAGCT 504 AGATAAATACCATCAAGAAT TGGAAATGAT TCACCATTCA AATGAGTATA ACATTGATAT 564 AACAGCGCAAAATATTAACA AATATACTGC ATTACAATAT ATATTTGATG CAGATGTTAA 624 ATATATAGCATTTGGTAATG ACCACAATGA TATTGTCATG TTACAACATG CTAGTAGTGG 684 CTATATTATAGGACCATCAG AAGCATACAC ACACGCAATA TTGAAACTTG ATAAAATCAA 744 ACACATCAATAATAATGCAC AAGCTATTTG CAAAGTCTTA AAATCATATA AATAA 799 128 amino acidsamino acid linear protein 76 Ser Trp Tyr Tyr Ile Val Trp Ser Asp Leu MetArg Phe Val Phe Asp 1 5 10 15 Ile Asp Gly Thr Leu Cys Phe Asp Gly ArgLeu Ile Asp Gln Thr Ile 20 25 30 Ile Asp Thr Leu Leu Gln Leu Gln His AspGly His Glu Leu Ile Phe 35 40 45 Ala Ser Ala Arg Pro Ile Arg Asp Leu LeuPro Val Leu Pro Ser Val 50 55 60 Phe His Gln His Thr Leu Ile Gly Ala AsnGly Ala Met Ile Ser Gln 65 70 75 80 Gln Ser Lys Ile Ser Val Ile Lys ProIle His Thr Asp Thr Tyr His 85 90 95 His Ile Phe Lys Ile Ile Gln Lys TyrGlu Leu Asp Tyr Ile Ile Asp 100 105 110 Asp Asp Trp Asn Tyr Ala Ala GlnLeu Asp Ala Xaa Glu Arg Asp Phe 115 120 125 799 base pairs nucleic acidsingle linear other nucleic acid /desc = ”DNA (genomic) (p11c66)“ CDS335..796 77 TCATGGTATT ATATTGTTTG GAGTGATTTG ATGAGATTTG TCTTTGATATTGATGGTACG 60 CTTTGTTTTG ACGGCCGATT AATTGACCAG ACTATTATTG ATACATTGTTACAATTACAA 120 CATGATGGTC ATGAACTTAT ATTTGCATCA GCACGTCCGA TTCGTGATTTGTTGCCAGTT 180 TTACCATCAG TATTTCATCA GCACACATTA ATTGGCGCAA ATGGTGCTATGATTTCACAG 240 CAATCAAAGA TTTCTGTTAT CAAACCAATT CATACTGATA CATATCATCATATCTTCAAA 300 ATAATTCAAA AGTATGAGTT AGATTATATT ATTG ATG ATG ATT GGA ATTATG 352 Met Met Ile Gly Ile Met 1 5 CTG CAC AAC TTG ACG CTG NAG AAC GCGATT TTT GAG CGT TTA GAT CCA 400 Leu His Asn Leu Thr Leu Xaa Asn Ala IlePhe Glu Arg Leu Asp Pro 10 15 20 CAT AAG CTG GCC AGT TGT ATT GAT GTT GCAAAT ATC GAC ACG CCA ATC 448 His Lys Leu Ala Ser Cys Ile Asp Val Ala AsnIle Asp Thr Pro Ile 25 30 35 AAG AKT ATT TTA TTA AAT ATA GAC CCG GCA CAAATT ACA ACT ATA TTA 496 Lys Xaa Ile Leu Leu Asn Ile Asp Pro Ala Gln IleThr Thr Ile Leu 40 45 50 GAC GAG CTA GAT AAA TAC CAT CAA GAA TTG GAA ATGATT CAC CAT TCA 544 Asp Glu Leu Asp Lys Tyr His Gln Glu Leu Glu Met IleHis His Ser 55 60 65 70 AAT GAG TAT AAC ATT GAT ATA ACA GCG CAA AAT ATTAAC AAA TAT ACT 592 Asn Glu Tyr Asn Ile Asp Ile Thr Ala Gln Asn Ile AsnLys Tyr Thr 75 80 85 GCA TTA CAA TAT ATA TTT GAT GCA GAT GTT AAA TAT ATAGCA TTT GGT 640 Ala Leu Gln Tyr Ile Phe Asp Ala Asp Val Lys Tyr Ile AlaPhe Gly 90 95 100 AAT GAC CAC AAT GAT ATT GTC ATG TTA CAA CAT GCT AGTAGT GGC TAT 688 Asn Asp His Asn Asp Ile Val Met Leu Gln His Ala Ser SerGly Tyr 105 110 115 ATT ATA GGA CCA TCA GAA GCA TAC ACA CAC GCA ATA TTGAAA CTT GAT 736 Ile Ile Gly Pro Ser Glu Ala Tyr Thr His Ala Ile Leu LysLeu Asp 120 125 130 AAA ATC AAA CAC ATC AAT AAT AAT GCA CAA GCT ATT TGCAAA GTC TTA 784 Lys Ile Lys His Ile Asn Asn Asn Ala Gln Ala Ile Cys LysVal Leu 135 140 145 150 AAA TCA TAT AAA TAA 799 Lys Ser Tyr Lys 154amino acids amino acid linear protein 78 Met Met Ile Gly Ile Met Leu HisAsn Leu Thr Leu Xaa Asn Ala Ile 1 5 10 15 Phe Glu Arg Leu Asp Pro HisLys Leu Ala Ser Cys Ile Asp Val Ala 20 25 30 Asn Ile Asp Thr Pro Ile LysXaa Ile Leu Leu Asn Ile Asp Pro Ala 35 40 45 Gln Ile Thr Thr Ile Leu AspGlu Leu Asp Lys Tyr His Gln Glu Leu 50 55 60 Glu Met Ile His His Ser AsnGlu Tyr Asn Ile Asp Ile Thr Ala Gln 65 70 75 80 Asn Ile Asn Lys Tyr ThrAla Leu Gln Tyr Ile Phe Asp Ala Asp Val 85 90 95 Lys Tyr Ile Ala Phe GlyAsn Asp His Asn Asp Ile Val Met Leu Gln 100 105 110 His Ala Ser Ser GlyTyr Ile Ile Gly Pro Ser Glu Ala Tyr Thr His 115 120 125 Ala Ile Leu LysLeu Asp Lys Ile Lys His Ile Asn Asn Asn Ala Gln 130 135 140 Ala Ile CysLys Val Leu Lys Ser Tyr Lys 145 150 1236 base pairs nucleic acid singlelinear other nucleic acid /desc = ”DNA (genomic) p5c34“ CDS 1..1233 79AGC TCT ACG TTC AAC ATT TTT CCA TTG GGG ATT CCA TGC TTG GGC TGT 48 SerSer Thr Phe Asn Ile Phe Pro Leu Gly Ile Pro Cys Leu Gly Cys 1 5 10 15TTA TGG TGT TGT TGC GTT ACG TTG GCA TAT TCG CAA TTC CGT AAA GGT 96 LeuTrp Cys Cys Cys Val Thr Leu Ala Tyr Ser Gln Phe Arg Lys Gly 20 25 30 GAACCA GGT TTA TTA TCT AGA ACT TTA CGT CCT CTT TTA GGT GAT AAA 144 Glu ProGly Leu Leu Ser Arg Thr Leu Arg Pro Leu Leu Gly Asp Lys 35 40 45 GTA GAAGGT CCT ATT GGG ATT TTT ATT GAC GTT TTA TCT GTA TTT GCG 192 Val Glu GlyPro Ile Gly Ile Phe Ile Asp Val Leu Ser Val Phe Ala 50 55 60 ACA ATC GTTGGG GTA GCC GTT TCG TTA GGT ATG GGT GCT CTA CAA ATT 240 Thr Ile Val GlyVal Ala Val Ser Leu Gly Met Gly Ala Leu Gln Ile 65 70 75 80 AAT GGT GGTTTA CAT TAC TTG TTC AAT GTT CCA AAC AAT ACG TTT GTA 288 Asn Gly Gly LeuHis Tyr Leu Phe Asn Val Pro Asn Asn Thr Phe Val 85 90 95 CAA GCG ATT ATCATC ATT GTT GTT ACT ATC TTA TTT ATA GCA AGT GCA 336 Gln Ala Ile Ile IleIle Val Val Thr Ile Leu Phe Ile Ala Ser Ala 100 105 110 TGG TCT GGA TTAAGT AAA GGT ATT CAA TAC TTA AGT AAC TTG AAC ATT 384 Trp Ser Gly Leu SerLys Gly Ile Gln Tyr Leu Ser Asn Leu Asn Ile 115 120 125 GGT TTA GGT ACTATT TTA ATG GTA GCT GCT TTA ATT GTT GGA CCA ACT 432 Gly Leu Gly Thr IleLeu Met Val Ala Ala Leu Ile Val Gly Pro Thr 130 135 140 GTT CTT ATT TTAAAT ATG TTA ACT AGC TCT ACG GGT AGT TTA CTA AAC 480 Val Leu Ile Leu AsnMet Leu Thr Ser Ser Thr Gly Ser Leu Leu Asn 145 150 155 160 ACA TTC TTGTTT AAT AGT TTT GAT ACA GCA GCT TTA AAT CCT CAA AAA 528 Thr Phe Leu PheAsn Ser Phe Asp Thr Ala Ala Leu Asn Pro Gln Lys 165 170 175 CGT GAA TGGATG TCT TCA TGG ACA CTT TAT TAC TGG GGT TGG TGG TTA 576 Arg Glu Trp MetSer Ser Trp Thr Leu Tyr Tyr Trp Gly Trp Trp Leu 180 185 190 AGT TGG AGTCCA TTC GTT GGA GTG TTT ATT GCA CGA GTT TCA AAA GGA 624 Ser Trp Ser ProPhe Val Gly Val Phe Ile Ala Arg Val Ser Lys Gly 195 200 205 CGT TCA ATTAGA GAG TTC ATT TCT GGT GTC TTG CTA GTT CCA GCA ATT 672 Arg Ser Ile ArgGlu Phe Ile Ser Gly Val Leu Leu Val Pro Ala Ile 210 215 220 GTT AGT TTTGTT TGG TTT AGT GTC TTT GGT GTA TTA GGC ATC GAG ACA 720 Val Ser Phe ValTrp Phe Ser Val Phe Gly Val Leu Gly Ile Glu Thr 225 230 235 240 GGT AAGAAA CAC AAA GAA ATT TTT GAT ATG ACT CCT GAA ACA CAG CTA 768 Gly Lys LysHis Lys Glu Ile Phe Asp Met Thr Pro Glu Thr Gln Leu 245 250 255 TTT GGAGTG TTT AAT CAT GTG CCA TTT GGC ATT GTT TTA TCG TTG ATT 816 Phe Gly ValPhe Asn His Val Pro Phe Gly Ile Val Leu Ser Leu Ile 260 265 270 GCA TTATTA TTA ATT GCA TCA TTC TTT ATT ACA TCT GCT GAC TCA GCA 864 Ala Leu LeuLeu Ile Ala Ser Phe Phe Ile Thr Ser Ala Asp Ser Ala 275 280 285 ACA TTTGTA TTA GGA ATG CAA ACA ACA TTT GGT TCA TTA AAT CCA TCT 912 Thr Phe ValLeu Gly Met Gln Thr Thr Phe Gly Ser Leu Asn Pro Ser 290 295 300 AGT ATGGTA AAA GTT GTT TGG GGA ATT TCA CAG GCC TTA ATA GCA TTT 960 Ser Met ValLys Val Val Trp Gly Ile Ser Gln Ala Leu Ile Ala Phe 305 310 315 320 GTACTT TTA TTA GCT GGT GGC GGT AAC GGC GCT GAA GCT TTA AAT GCG 1008 Val LeuLeu Leu Ala Gly Gly Gly Asn Gly Ala Glu Ala Leu Asn Ala 325 330 335 ATTCAA AGT GCT GCA ATT ATA AGT GCA TTC CCA TTC TCC TTT GTC GTC 1056 Ile GlnSer Ala Ala Ile Ile Ser Ala Phe Pro Phe Ser Phe Val Val 340 345 350 ATACTC ATG ATG GTA AGT TTC TAC AAG GAT GCG AAC CAG GAA CGT AAA 1104 Ile LeuMet Met Val Ser Phe Tyr Lys Asp Ala Asn Gln Glu Arg Lys 355 360 365 TTCCTA GGT TTA ACA TTG ACT CCG AAT AAA CAT CGC TTA CAA GAA TAT 1152 Phe LeuGly Leu Thr Leu Thr Pro Asn Lys His Arg Leu Gln Glu Tyr 370 375 380 ATCAAG AGT CAA CAA GAA GAT TAT GAA TCT GAC ATT CTT GAA AAG CGT 1200 Ile LysSer Gln Gln Glu Asp Tyr Glu Ser Asp Ile Leu Glu Lys Arg 385 390 395 400CAG TCA CGT AGA AAT ATA GAG AAA AAA GAT AAC TAA 1236 Gln Ser Arg Arg AsnIle Glu Lys Lys Asp Asn 405 410 411 amino acids amino acid linearprotein 80 Ser Ser Thr Phe Asn Ile Phe Pro Leu Gly Ile Pro Cys Leu GlyCys 1 5 10 15 Leu Trp Cys Cys Cys Val Thr Leu Ala Tyr Ser Gln Phe ArgLys Gly 20 25 30 Glu Pro Gly Leu Leu Ser Arg Thr Leu Arg Pro Leu Leu GlyAsp Lys 35 40 45 Val Glu Gly Pro Ile Gly Ile Phe Ile Asp Val Leu Ser ValPhe Ala 50 55 60 Thr Ile Val Gly Val Ala Val Ser Leu Gly Met Gly Ala LeuGln Ile 65 70 75 80 Asn Gly Gly Leu His Tyr Leu Phe Asn Val Pro Asn AsnThr Phe Val 85 90 95 Gln Ala Ile Ile Ile Ile Val Val Thr Ile Leu Phe IleAla Ser Ala 100 105 110 Trp Ser Gly Leu Ser Lys Gly Ile Gln Tyr Leu SerAsn Leu Asn Ile 115 120 125 Gly Leu Gly Thr Ile Leu Met Val Ala Ala LeuIle Val Gly Pro Thr 130 135 140 Val Leu Ile Leu Asn Met Leu Thr Ser SerThr Gly Ser Leu Leu Asn 145 150 155 160 Thr Phe Leu Phe Asn Ser Phe AspThr Ala Ala Leu Asn Pro Gln Lys 165 170 175 Arg Glu Trp Met Ser Ser TrpThr Leu Tyr Tyr Trp Gly Trp Trp Leu 180 185 190 Ser Trp Ser Pro Phe ValGly Val Phe Ile Ala Arg Val Ser Lys Gly 195 200 205 Arg Ser Ile Arg GluPhe Ile Ser Gly Val Leu Leu Val Pro Ala Ile 210 215 220 Val Ser Phe ValTrp Phe Ser Val Phe Gly Val Leu Gly Ile Glu Thr 225 230 235 240 Gly LysLys His Lys Glu Ile Phe Asp Met Thr Pro Glu Thr Gln Leu 245 250 255 PheGly Val Phe Asn His Val Pro Phe Gly Ile Val Leu Ser Leu Ile 260 265 270Ala Leu Leu Leu Ile Ala Ser Phe Phe Ile Thr Ser Ala Asp Ser Ala 275 280285 Thr Phe Val Leu Gly Met Gln Thr Thr Phe Gly Ser Leu Asn Pro Ser 290295 300 Ser Met Val Lys Val Val Trp Gly Ile Ser Gln Ala Leu Ile Ala Phe305 310 315 320 Val Leu Leu Leu Ala Gly Gly Gly Asn Gly Ala Glu Ala LeuAsn Ala 325 330 335 Ile Gln Ser Ala Ala Ile Ile Ser Ala Phe Pro Phe SerPhe Val Val 340 345 350 Ile Leu Met Met Val Ser Phe Tyr Lys Asp Ala AsnGln Glu Arg Lys 355 360 365 Phe Leu Gly Leu Thr Leu Thr Pro Asn Lys HisArg Leu Gln Glu Tyr 370 375 380 Ile Lys Ser Gln Gln Glu Asp Tyr Glu SerAsp Ile Leu Glu Lys Arg 385 390 395 400 Gln Ser Arg Arg Asn Ile Glu LysLys Asp Asn 405 410 477 base pairs nucleic acid single linear othernucleic acid /desc = ”DNA (genomic) (p10c18)“ 81 ATTATAGAGT TTTTCCGCTGTTTCTAAAGT TAAATTATTC ACTTTTCTTT TCCCGCTTCT 60 TAATTCACTT ATACCACCATAAGAAACATC AGTGTCTTGA TTAATTCTAT AATTAGATAT 120 TGATYTATCA TTTAGTARTTTTTCTATTGT ATTATNAATT TCTTTAARCT GGTTCWTAAT 180 TTTNGTCNAA ATGAAAGAATAATTTATTTT NTCTCTANGT TATATTAATC AATNAANTAA 240 TATTANAGTT GCAANTTAAGNATAGAGAGT TNATTTTTTT CCTTTANATT CCTCCTTGGT 300 CNCTNAANAT TANCCANCCTNCCCCTTATT TTTAAATTTG GTGGGTNNAA AGGGGTTNTT 360 CTNGGCCCCN TTTTTNCCCTNTTTTTTTTN ANAAAGNANC CGNAATTCTG GATCCTAACT 420 TTTANTNTTN NCTTGGGAACTCTAATTCAA GGGNCCTCNG CTCGNCNAGC NCTAATT 477 1212 base pairs nucleicacid single linear other nucleic acid /desc = ”DNA (genomic) (p12c3)“CDS 1..1209 82 GAA AGA AAG CGT TTT CAC ATA ACA AAG GGG GAG TTT CAA ATGAAA GTC 48 Glu Arg Lys Arg Phe His Ile Thr Lys Gly Glu Phe Gln Met LysVal 1 5 10 15 GAA GTT TAT AAA GGA GCG CAA GGT AAA CAT AAC CTT AAA GATTAT GAA 96 Glu Val Tyr Lys Gly Ala Gln Gly Lys His Asn Leu Lys Asp TyrGlu 20 25 30 GAA ACA TAT AAT ACT TTT GAT TGG AAA GAC GTA GAA CAA GCA TTTTCT 144 Glu Thr Tyr Asn Thr Phe Asp Trp Lys Asp Val Glu Gln Ala Phe Ser35 40 45 TGG AGT GAA ACT GGA AAA ATG AAC ATG GCA TAT GAA TGC ATA GAT CGC192 Trp Ser Glu Thr Gly Lys Met Asn Met Ala Tyr Glu Cys Ile Asp Arg 5055 60 CAT GTA GAT CAA GGA TTA GGG GAT AAA ATA GCG TTA AAT TAC AAA GAT240 His Val Asp Gln Gly Leu Gly Asp Lys Ile Ala Leu Asn Tyr Lys Asp 6570 75 80 GAG CAC AGA AAA GAA TCG TAT ACT TAT AAA GAT ATG CAA CGG TTA TCT288 Glu His Arg Lys Glu Ser Tyr Thr Tyr Lys Asp Met Gln Arg Leu Ser 8590 95 AAT AAA GCA GCG AAT GTT TTG TCT GAA CAT GCA GAA GTT GAC AAA GGT336 Asn Lys Ala Ala Asn Val Leu Ser Glu His Ala Glu Val Asp Lys Gly 100105 110 GAC AGA GTA TTT ATA TTT ATG TCG CGT ACA CCT GAA CTA TAT TTT GCG384 Asp Arg Val Phe Ile Phe Met Ser Arg Thr Pro Glu Leu Tyr Phe Ala 115120 125 TTG TTA GGT GTT TTA AAA ATT GGT GCA ATT GTT GGG CCG TTA TTT GAA432 Leu Leu Gly Val Leu Lys Ile Gly Ala Ile Val Gly Pro Leu Phe Glu 130135 140 GCA TTT ATG GAA AAG GCA GTT GCG GAT AGA TTA GAG AAC AGT GAA GCT480 Ala Phe Met Glu Lys Ala Val Ala Asp Arg Leu Glu Asn Ser Glu Ala 145150 155 160 AAA GTG TTA ATT ACT AAT AAG GCA TTG TTA CCT CGA GTA CCT GTAGAT 528 Lys Val Leu Ile Thr Asn Lys Ala Leu Leu Pro Arg Val Pro Val Asp165 170 175 AAA TTA CCA AAC TTG AAA AAA ATT GTT GTC GTA GAT GAG GAT GTAGAA 576 Lys Leu Pro Asn Leu Lys Lys Ile Val Val Val Asp Glu Asp Val Glu180 185 190 GAC AAT TAC ATA GAC TTC ATT AGT TTG ATG GAA ACT GCT AGC GATGAA 624 Asp Asn Tyr Ile Asp Phe Ile Ser Leu Met Glu Thr Ala Ser Asp Glu195 200 205 TTT GAC ATT GAA TGG TTA AAG TCG GAT GAT GGT TTG ATT TTA CATTAT 672 Phe Asp Ile Glu Trp Leu Lys Ser Asp Asp Gly Leu Ile Leu His Tyr210 215 220 ACA TCA GGT TCT ACT GGG CAA CCT AAA GGT GTA TTG CAT GTT CAACAA 720 Thr Ser Gly Ser Thr Gly Gln Pro Lys Gly Val Leu His Val Gln Gln225 230 235 240 GCA ATG TTA GTG CAC TAT ATT TCT GGR AAA TAT GTA TTA GATTTA CAA 768 Ala Met Leu Val His Tyr Ile Ser Gly Lys Tyr Val Leu Asp LeuGln 245 250 255 GAA GAT GAT GTT TAT TGG TGT ACA GCA GAT CCA GGT TGG GTTACA GGA 816 Glu Asp Asp Val Tyr Trp Cys Thr Ala Asp Pro Gly Trp Val ThrGly 260 265 270 ACA TCT TAT GGT ATT TTT GCA CCA TGG TTA AAT GGC GCT ACAAAT TGT 864 Thr Ser Tyr Gly Ile Phe Ala Pro Trp Leu Asn Gly Ala Thr AsnCys 275 280 285 ATA GCT GGT GGT CGC TTT TCG CCA GAA CAG TGG TAT AGT ATGATT GAA 912 Ile Ala Gly Gly Arg Phe Ser Pro Glu Gln Trp Tyr Ser Met IleGlu 290 295 300 GAT TTT AAA GTG ACG ATT TGG TAT ACG GCA CCA ACA GCT TTAAGA ATG 960 Asp Phe Lys Val Thr Ile Trp Tyr Thr Ala Pro Thr Ala Leu ArgMet 305 310 315 320 TTA ATG AGT GCT GGT GAC GAT ATT GTT GAG AAA TAT GACTTG TCA TCG 1008 Leu Met Ser Ala Gly Asp Asp Ile Val Glu Lys Tyr Asp LeuSer Ser 325 330 335 TTA CGT TCG ATT CTA TCA GTA GGT GAG CCT TTA AAT CCTGAA GTT ATA 1056 Leu Arg Ser Ile Leu Ser Val Gly Glu Pro Leu Asn Pro GluVal Ile 340 345 350 AAA TGG GCG AAA AAA GTA TAC GGT TTA ACG GTG TTA GATACT TGG TGG 1104 Lys Trp Ala Lys Lys Val Tyr Gly Leu Thr Val Leu Asp ThrTrp Trp 355 360 365 ATG ACA GAA ACA GGT GGA CAT ATG ATT GTT AAC TAT CCAACG ATG GAC 1152 Met Thr Glu Thr Gly Gly His Met Ile Val Asn Tyr Pro ThrMet Asp 370 375 380 GTN CAA GCT TGG CTC MAT GGG CAA ACC ATT ACC TGG TATTCA AGC TGC 1200 Val Gln Ala Trp Leu Xaa Gly Gln Thr Ile Thr Trp Tyr SerSer Cys 385 390 395 400 AAT TAT CGA TGA 1212 Asn Tyr Arg 403 amino acidsamino acid linear protein 83 Glu Arg Lys Arg Phe His Ile Thr Lys Gly GluPhe Gln Met Lys Val 1 5 10 15 Glu Val Tyr Lys Gly Ala Gln Gly Lys HisAsn Leu Lys Asp Tyr Glu 20 25 30 Glu Thr Tyr Asn Thr Phe Asp Trp Lys AspVal Glu Gln Ala Phe Ser 35 40 45 Trp Ser Glu Thr Gly Lys Met Asn Met AlaTyr Glu Cys Ile Asp Arg 50 55 60 His Val Asp Gln Gly Leu Gly Asp Lys IleAla Leu Asn Tyr Lys Asp 65 70 75 80 Glu His Arg Lys Glu Ser Tyr Thr TyrLys Asp Met Gln Arg Leu Ser 85 90 95 Asn Lys Ala Ala Asn Val Leu Ser GluHis Ala Glu Val Asp Lys Gly 100 105 110 Asp Arg Val Phe Ile Phe Met SerArg Thr Pro Glu Leu Tyr Phe Ala 115 120 125 Leu Leu Gly Val Leu Lys IleGly Ala Ile Val Gly Pro Leu Phe Glu 130 135 140 Ala Phe Met Glu Lys AlaVal Ala Asp Arg Leu Glu Asn Ser Glu Ala 145 150 155 160 Lys Val Leu IleThr Asn Lys Ala Leu Leu Pro Arg Val Pro Val Asp 165 170 175 Lys Leu ProAsn Leu Lys Lys Ile Val Val Val Asp Glu Asp Val Glu 180 185 190 Asp AsnTyr Ile Asp Phe Ile Ser Leu Met Glu Thr Ala Ser Asp Glu 195 200 205 PheAsp Ile Glu Trp Leu Lys Ser Asp Asp Gly Leu Ile Leu His Tyr 210 215 220Thr Ser Gly Ser Thr Gly Gln Pro Lys Gly Val Leu His Val Gln Gln 225 230235 240 Ala Met Leu Val His Tyr Ile Ser Gly Lys Tyr Val Leu Asp Leu Gln245 250 255 Glu Asp Asp Val Tyr Trp Cys Thr Ala Asp Pro Gly Trp Val ThrGly 260 265 270 Thr Ser Tyr Gly Ile Phe Ala Pro Trp Leu Asn Gly Ala ThrAsn Cys 275 280 285 Ile Ala Gly Gly Arg Phe Ser Pro Glu Gln Trp Tyr SerMet Ile Glu 290 295 300 Asp Phe Lys Val Thr Ile Trp Tyr Thr Ala Pro ThrAla Leu Arg Met 305 310 315 320 Leu Met Ser Ala Gly Asp Asp Ile Val GluLys Tyr Asp Leu Ser Ser 325 330 335 Leu Arg Ser Ile Leu Ser Val Gly GluPro Leu Asn Pro Glu Val Ile 340 345 350 Lys Trp Ala Lys Lys Val Tyr GlyLeu Thr Val Leu Asp Thr Trp Trp 355 360 365 Met Thr Glu Thr Gly Gly HisMet Ile Val Asn Tyr Pro Thr Met Asp 370 375 380 Val Gln Ala Trp Leu XaaGly Gln Thr Ile Thr Trp Tyr Ser Ser Cys 385 390 395 400 Asn Tyr Arg 3147base pairs nucleic acid single linear other nucleic acid /desc = ”DNA(genomic) (p14b25)“ CDS 1..3144 84 ATG AAA ATT CTC AGC CTG CGC CTG AAAAAC CTG AAC TCA TTA AAA GGC 48 Met Lys Ile Leu Ser Leu Arg Leu Lys AsnLeu Asn Ser Leu Lys Gly 1 5 10 15 GAA TGG AAG ATT GAT TTC ACC CGC GAGCCG TTC GCC AGC AAC GGG CTG 96 Glu Trp Lys Ile Asp Phe Thr Arg Glu ProPhe Ala Ser Asn Gly Leu 20 25 30 TTT GCT ATT ACC GGC CCA ACA GGT GCG GGGAAA ACC ACC CTG CTG GAC 144 Phe Ala Ile Thr Gly Pro Thr Gly Ala Gly LysThr Thr Leu Leu Asp 35 40 45 GCC ATT TGT CTG GCG CTG TAT CAC GAA ACT CCGCGT CTC TCT AAC GTT 192 Ala Ile Cys Leu Ala Leu Tyr His Glu Thr Pro ArgLeu Ser Asn Val 50 55 60 TCA CAA TCG CAA AAT GAT CTC ATG ACC CGC GAT ACCGCC GAA TGT CTG 240 Ser Gln Ser Gln Asn Asp Leu Met Thr Arg Asp Thr AlaGlu Cys Leu 65 70 75 80 GCG GAG GTG GAG TTT GAA GTG AAA GGT GAA GCG TACCGT GCA TTC TGG 288 Ala Glu Val Glu Phe Glu Val Lys Gly Glu Ala Tyr ArgAla Phe Trp 85 90 95 AGC CAG AAT CGG GCG CGT AAC CAA CCC GAC GGT AAT TTGCAG GTG CCA 336 Ser Gln Asn Arg Ala Arg Asn Gln Pro Asp Gly Asn Leu GlnVal Pro 100 105 110 CGC GTA GAG CTG GCG CGC TGC GCC GAC GGC AAA ATT CTCGCC GAC AAA 384 Arg Val Glu Leu Ala Arg Cys Ala Asp Gly Lys Ile Leu AlaAsp Lys 115 120 125 GTG AAA GAT AAG CTG GAA CTG ACA GCG ACG TTA ACC GGGCTG GAT TAC 432 Val Lys Asp Lys Leu Glu Leu Thr Ala Thr Leu Thr Gly LeuAsp Tyr 130 135 140 GGG CGC TTC ACC CGT TCG ATG CTG CTT TCG CAG GGG CAATTT GCT GCC 480 Gly Arg Phe Thr Arg Ser Met Leu Leu Ser Gln Gly Gln PheAla Ala 145 150 155 160 TTC CTG AAT GCC AAA CCC AAA GAA CGC GCG GAA TTGCTC GAG GAG TTA 528 Phe Leu Asn Ala Lys Pro Lys Glu Arg Ala Glu Leu LeuGlu Glu Leu 165 170 175 ACC GGC ACT GAA ATC TAC GGG CAA ATC TCG GCG ATGGTT TTT GAG CAG 576 Thr Gly Thr Glu Ile Tyr Gly Gln Ile Ser Ala Met ValPhe Glu Gln 180 185 190 CAC AAA TCG GCC CGC ACA GAG CTG GAG AAG CTG CAAGCG CAG GCC AGC 624 His Lys Ser Ala Arg Thr Glu Leu Glu Lys Leu Gln AlaGln Ala Ser 195 200 205 GGC GTC ACG TTG CTC ACG CCG GAA CAA GTG CAA TCGCTG ACA GCG AGT 672 Gly Val Thr Leu Leu Thr Pro Glu Gln Val Gln Ser LeuThr Ala Ser 210 215 220 TTG CAG GTA CTT ACT GAC GAA GAA AAA CAG TTA ATTACC GCG CAG CAG 720 Leu Gln Val Leu Thr Asp Glu Glu Lys Gln Leu Ile ThrAla Gln Gln 225 230 235 240 CAA GAA CAA CAA TCG CTA AAC TGG TTA ACG CGTCAG GAC GAA TTG CAG 768 Gln Glu Gln Gln Ser Leu Asn Trp Leu Thr Arg GlnAsp Glu Leu Gln 245 250 255 CAA GAA GCC AGC CGC CGT CAG CAG GCC TTG CAACAG GCG TTA GCC GAA 816 Gln Glu Ala Ser Arg Arg Gln Gln Ala Leu Gln GlnAla Leu Ala Glu 260 265 270 GAA GAA AAA GCG CAA CCT CAA CTG GCG GCG CTTAGT CTG GCA CAA CCG 864 Glu Glu Lys Ala Gln Pro Gln Leu Ala Ala Leu SerLeu Ala Gln Pro 275 280 285 GCA CGA AAT CTT CGT CCA CAC TGG GAA CGC ATCGCA GAA CAC AGC GCG 912 Ala Arg Asn Leu Arg Pro His Trp Glu Arg Ile AlaGlu His Ser Ala 290 295 300 GCG CTG GCG CAT ATT CGC CAG CAG ATT GAA GAAGTA AAT ACT CGC TTA 960 Ala Leu Ala His Ile Arg Gln Gln Ile Glu Glu ValAsn Thr Arg Leu 305 310 315 320 CAG AGC ACA ATG GCG CTT CGC GCG AGC ATTCGC CAC CAC GCG GCG AAG 1008 Gln Ser Thr Met Ala Leu Arg Ala Ser Ile ArgHis His Ala Ala Lys 325 330 335 CAG TCA GCA GAA TTA CAG CAG CAG CAA CAAAGC CTG AAT ACC TGG TTA 1056 Gln Ser Ala Glu Leu Gln Gln Gln Gln Gln SerLeu Asn Thr Trp Leu 340 345 350 CAG GAA CAC GAC CGC TTC CGT CAG TGG AACAAC GAA CCG GCG GGT TGG 1104 Gln Glu His Asp Arg Phe Arg Gln Trp Asn AsnGlu Pro Ala Gly Trp 355 360 365 CGT GCG CAG TTC TCC CAA CAA ACC AGC GATCGC GAG CAT CTG CGG CAA 1152 Arg Ala Gln Phe Ser Gln Gln Thr Ser Asp ArgGlu His Leu Arg Gln 370 375 380 TGG CAG CAA CAG TTA ACC CAT GCT GAG CAAAAA CTT AAT GCG CTT GCG 1200 Trp Gln Gln Gln Leu Thr His Ala Glu Gln LysLeu Asn Ala Leu Ala 385 390 395 400 GCG ATC ACG TTG ACG TTA ACC GCC GATGAA GTT GCT ACC GCC CTG GCG 1248 Ala Ile Thr Leu Thr Leu Thr Ala Asp GluVal Ala Thr Ala Leu Ala 405 410 415 CAA CAT GCT GAG CAA CGC CCA CTG CGTCAG CAC CTG GTC GCG CTG CAT 1296 Gln His Ala Glu Gln Arg Pro Leu Arg GlnHis Leu Val Ala Leu His 420 425 430 GGA CAG ATT GTT CCC CAA CAA AAA CGTCTG GCG CAG TTA CAG GTC GCT 1344 Gly Gln Ile Val Pro Gln Gln Lys Arg LeuAla Gln Leu Gln Val Ala 435 440 445 ATC CAG AAT GTC ACG CAA GAA CAG ACGCAA CGT AAC GCC GCA CTT AAC 1392 Ile Gln Asn Val Thr Gln Glu Gln Thr GlnArg Asn Ala Ala Leu Asn 450 455 460 GAA ATG CGC CAG CGT TAT AAA GAA AAGACG CAG CAA CTT GCC GAT GTG 1440 Glu Met Arg Gln Arg Tyr Lys Glu Lys ThrGln Gln Leu Ala Asp Val 465 470 475 480 AAA ACC ATT TGC GAG CAG GAA GCGCGC ATC AAA ACG CTG GAA GCT CAA 1488 Lys Thr Ile Cys Glu Gln Glu Ala ArgIle Lys Thr Leu Glu Ala Gln 485 490 495 CGT GCA CAG TTA CAG GCG GGT CAGCCT TGC CCA CTT TGT GGT TCC ACC 1536 Arg Ala Gln Leu Gln Ala Gly Gln ProCys Pro Leu Cys Gly Ser Thr 500 505 510 AGC CAC CCG GCG GTC GAG GCG TATCAG GCG CTG GAG CCT GGC GTT AAT 1584 Ser His Pro Ala Val Glu Ala Tyr GlnAla Leu Glu Pro Gly Val Asn 515 520 525 CAG TCT CGA TTA CTG GCG CTG GAAAAC GAA GTT AAA AAG CTC GGT GAA 1632 Gln Ser Arg Leu Leu Ala Leu Glu AsnGlu Val Lys Lys Leu Gly Glu 530 535 540 GAA GGT GCG ACG CTA CGT GGG CAACTG GAC GCC ATA ACA AAG CAG CTT 1680 Glu Gly Ala Thr Leu Arg Gly Gln LeuAsp Ala Ile Thr Lys Gln Leu 545 550 555 560 CAG CGT GAT GAA AAC GAA GCGCAA AGC CTC CGA CAA GAT GAG CAA GCA 1728 Gln Arg Asp Glu Asn Glu Ala GlnSer Leu Arg Gln Asp Glu Gln Ala 565 570 575 CTT ACT CAA CAA TGG CAA GCCGTC ACG GCC AGC CTC AAT ATC ACC TTG 1776 Leu Thr Gln Gln Trp Gln Ala ValThr Ala Ser Leu Asn Ile Thr Leu 580 585 590 CAG CCA CTG GAC GAT ATT CAACCG TGG CTG GAT GCA CAA GAT GAG CAC 1824 Gln Pro Leu Asp Asp Ile Gln ProTrp Leu Asp Ala Gln Asp Glu His 595 600 605 GAA CGC CAG CTG CGG TTA CTCAGC CAA CGG CAT GAA TTA CAA GGG CAG 1872 Glu Arg Gln Leu Arg Leu Leu SerGln Arg His Glu Leu Gln Gly Gln 610 615 620 ATT GCC GCG CAT AAT CAG CAAATT ATC CAG TAT CAA CAG CAA ATT GAA 1920 Ile Ala Ala His Asn Gln Gln IleIle Gln Tyr Gln Gln Gln Ile Glu 625 630 635 640 CAA CGC CAG CAA CTA CTTTTA ACG ACA TTG ACG GGT TAT GCA CTG ACA 1968 Gln Arg Gln Gln Leu Leu LeuThr Thr Leu Thr Gly Tyr Ala Leu Thr 645 650 655 TTG CCA CAG GAA GAT GAAGAA GAG AGC TGG TTG GCG ACA CGT CAG CAA 2016 Leu Pro Gln Glu Asp Glu GluGlu Ser Trp Leu Ala Thr Arg Gln Gln 660 665 670 GAA GCG CAG AGC TGG CAGCAA CGC CAG AAC GAA TTA ACC GCG CTG CAA 2064 Glu Ala Gln Ser Trp Gln GlnArg Gln Asn Glu Leu Thr Ala Leu Gln 675 680 685 AAC CGT ATT CAG CAG CTGACG CCG ATT CTG GAA ACG TTG CCG CAA AGT 2112 Asn Arg Ile Gln Gln Leu ThrPro Ile Leu Glu Thr Leu Pro Gln Ser 690 695 700 GAT GAA CTC CCG CAC TGCGAA GAA ACT GTG GTA TTG GAA AAC TGG CGG 2160 Asp Glu Leu Pro His Cys GluGlu Thr Val Val Leu Glu Asn Trp Arg 705 710 715 720 CAG GTA CAT GAA CAATGT CTC GCA TTA CAC AGC CAG CAG CAG ACG TTA 2208 Gln Val His Glu Gln CysLeu Ala Leu His Ser Gln Gln Gln Thr Leu 725 730 735 CAG CAA CAG GAT GTTCTG GCG GCG CAA AGT CTG CAA AAA GCC CAG GCG 2256 Gln Gln Gln Asp Val LeuAla Ala Gln Ser Leu Gln Lys Ala Gln Ala 740 745 750 CAG TTT GAC ACC GCGCTA CAG GCC AGC GTC TTT GAC GAT CAG CAG GCG 2304 Gln Phe Asp Thr Ala LeuGln Ala Ser Val Phe Asp Asp Gln Gln Ala 755 760 765 TTC CTT GCG GCG CTAATG GAT GAA CAA ACA CTA ACG CAG CTG GAA CAG 2352 Phe Leu Ala Ala Leu MetAsp Glu Gln Thr Leu Thr Gln Leu Glu Gln 770 775 780 CTC AAG CAG AAT CTGGAA AAC CAG CGC CGT CAG GCG CAA ACT CTG GTC 2400 Leu Lys Gln Asn Leu GluAsn Gln Arg Arg Gln Ala Gln Thr Leu Val 785 790 795 800 ACT CAG ACA GCAGAA ACG CTG GCA CAG CAT CAA CAA CAC CGA CCT GAC 2448 Thr Gln Thr Ala GluThr Leu Ala Gln His Gln Gln His Arg Pro Asp 805 810 815 GAC GGG TTG GCTCTC ACT GTG ACG GTG GAG CAG ATT CAG CAA GAG TTA 2496 Asp Gly Leu Ala LeuThr Val Thr Val Glu Gln Ile Gln Gln Glu Leu 820 825 830 GCG CAA ACT CACCAA AAG TTG CGT GAA AAC ACC ACG AGT CAA GGC GAG 2544 Ala Gln Thr His GlnLys Leu Arg Glu Asn Thr Thr Ser Gln Gly Glu 835 840 845 ATT CGC CAG CAGCTG AAG CAG GAT GCA GAT AAC CGT CAG CAA CAA CAA 2592 Ile Arg Gln Gln LeuLys Gln Asp Ala Asp Asn Arg Gln Gln Gln Gln 850 855 860 ACC TTA ATG CAGCAA ATT GCT CAA ATG ACG CAG CAG GTT GAG GAC TGG 2640 Thr Leu Met Gln GlnIle Ala Gln Met Thr Gln Gln Val Glu Asp Trp 865 870 875 880 GGA TAT CTGAAT TCG CTA ATA GGT TCC AAA GAG GGC GAT AAA TTC CGC 2688 Gly Tyr Leu AsnSer Leu Ile Gly Ser Lys Glu Gly Asp Lys Phe Arg 885 890 895 AAG TTT GCCCAG GGG CTG ACG CTG GAT AAT TTA GTC CAT CTC GCT AAT 2736 Lys Phe Ala GlnGly Leu Thr Leu Asp Asn Leu Val His Leu Ala Asn 900 905 910 CAG CAA CTTACC CGG CTG CAC GGG CGC TAT CTG TTA CAG CGC AAA GCC 2784 Gln Gln Leu ThrArg Leu His Gly Arg Tyr Leu Leu Gln Arg Lys Ala 915 920 925 AGC GAG GCGCTG GAA GTC GAG GTT GTT GAT ACC TGG CAG GCA GAT GCG 2832 Ser Glu Ala LeuGlu Val Glu Val Val Asp Thr Trp Gln Ala Asp Ala 930 935 940 GTA CGC GATACC CGT ACC CTT TCC GGC GGC GAA AGT TTC CTC GTT AGT 2880 Val Arg Asp ThrArg Thr Leu Ser Gly Gly Glu Ser Phe Leu Val Ser 945 950 955 960 CTG GCGCTG GCG CTG GCG CTT TCG GAT CTG GTC AGC CAT AAA ACA CGT 2928 Leu Ala LeuAla Leu Ala Leu Ser Asp Leu Val Ser His Lys Thr Arg 965 970 975 ATT GACTCG CTG TTC CTT GAT GAA GGT TTT GGC ACG CTG GAT AGC GAA 2976 Ile Asp SerLeu Phe Leu Asp Glu Gly Phe Gly Thr Leu Asp Ser Glu 980 985 990 ACG CTGGAT ACC GCC CTT GAT GCG CTG GAT GCC CTG AAC GCC AGT GGC 3024 Thr Leu AspThr Ala Leu Asp Ala Leu Asp Ala Leu Asn Ala Ser Gly 995 1000 1005 AAAACC ATC GGT GTG ATT AGC CAC GTA GAA GCG ATG AAA GAG CGT ATT 3072 Lys ThrIle Gly Val Ile Ser His Val Glu Ala Met Lys Glu Arg Ile 1010 1015 1020CCG GTG CAG ATC AAA GTG AAA AAG ATC AAC GGC CTG GGC TAC AGC AAA 3120 ProVal Gln Ile Lys Val Lys Lys Ile Asn Gly Leu Gly Tyr Ser Lys 1025 10301035 1040 CTG GAA AGT ACG TTT GCA GTG AAA TAA 3147 Leu Glu Ser Thr PheAla Val Lys 1045 1048 amino acids amino acid linear protein 85 Met LysIle Leu Ser Leu Arg Leu Lys Asn Leu Asn Ser Leu Lys Gly 1 5 10 15 GluTrp Lys Ile Asp Phe Thr Arg Glu Pro Phe Ala Ser Asn Gly Leu 20 25 30 PheAla Ile Thr Gly Pro Thr Gly Ala Gly Lys Thr Thr Leu Leu Asp 35 40 45 AlaIle Cys Leu Ala Leu Tyr His Glu Thr Pro Arg Leu Ser Asn Val 50 55 60 SerGln Ser Gln Asn Asp Leu Met Thr Arg Asp Thr Ala Glu Cys Leu 65 70 75 80Ala Glu Val Glu Phe Glu Val Lys Gly Glu Ala Tyr Arg Ala Phe Trp 85 90 95Ser Gln Asn Arg Ala Arg Asn Gln Pro Asp Gly Asn Leu Gln Val Pro 100 105110 Arg Val Glu Leu Ala Arg Cys Ala Asp Gly Lys Ile Leu Ala Asp Lys 115120 125 Val Lys Asp Lys Leu Glu Leu Thr Ala Thr Leu Thr Gly Leu Asp Tyr130 135 140 Gly Arg Phe Thr Arg Ser Met Leu Leu Ser Gln Gly Gln Phe AlaAla 145 150 155 160 Phe Leu Asn Ala Lys Pro Lys Glu Arg Ala Glu Leu LeuGlu Glu Leu 165 170 175 Thr Gly Thr Glu Ile Tyr Gly Gln Ile Ser Ala MetVal Phe Glu Gln 180 185 190 His Lys Ser Ala Arg Thr Glu Leu Glu Lys LeuGln Ala Gln Ala Ser 195 200 205 Gly Val Thr Leu Leu Thr Pro Glu Gln ValGln Ser Leu Thr Ala Ser 210 215 220 Leu Gln Val Leu Thr Asp Glu Glu LysGln Leu Ile Thr Ala Gln Gln 225 230 235 240 Gln Glu Gln Gln Ser Leu AsnTrp Leu Thr Arg Gln Asp Glu Leu Gln 245 250 255 Gln Glu Ala Ser Arg ArgGln Gln Ala Leu Gln Gln Ala Leu Ala Glu 260 265 270 Glu Glu Lys Ala GlnPro Gln Leu Ala Ala Leu Ser Leu Ala Gln Pro 275 280 285 Ala Arg Asn LeuArg Pro His Trp Glu Arg Ile Ala Glu His Ser Ala 290 295 300 Ala Leu AlaHis Ile Arg Gln Gln Ile Glu Glu Val Asn Thr Arg Leu 305 310 315 320 GlnSer Thr Met Ala Leu Arg Ala Ser Ile Arg His His Ala Ala Lys 325 330 335Gln Ser Ala Glu Leu Gln Gln Gln Gln Gln Ser Leu Asn Thr Trp Leu 340 345350 Gln Glu His Asp Arg Phe Arg Gln Trp Asn Asn Glu Pro Ala Gly Trp 355360 365 Arg Ala Gln Phe Ser Gln Gln Thr Ser Asp Arg Glu His Leu Arg Gln370 375 380 Trp Gln Gln Gln Leu Thr His Ala Glu Gln Lys Leu Asn Ala LeuAla 385 390 395 400 Ala Ile Thr Leu Thr Leu Thr Ala Asp Glu Val Ala ThrAla Leu Ala 405 410 415 Gln His Ala Glu Gln Arg Pro Leu Arg Gln His LeuVal Ala Leu His 420 425 430 Gly Gln Ile Val Pro Gln Gln Lys Arg Leu AlaGln Leu Gln Val Ala 435 440 445 Ile Gln Asn Val Thr Gln Glu Gln Thr GlnArg Asn Ala Ala Leu Asn 450 455 460 Glu Met Arg Gln Arg Tyr Lys Glu LysThr Gln Gln Leu Ala Asp Val 465 470 475 480 Lys Thr Ile Cys Glu Gln GluAla Arg Ile Lys Thr Leu Glu Ala Gln 485 490 495 Arg Ala Gln Leu Gln AlaGly Gln Pro Cys Pro Leu Cys Gly Ser Thr 500 505 510 Ser His Pro Ala ValGlu Ala Tyr Gln Ala Leu Glu Pro Gly Val Asn 515 520 525 Gln Ser Arg LeuLeu Ala Leu Glu Asn Glu Val Lys Lys Leu Gly Glu 530 535 540 Glu Gly AlaThr Leu Arg Gly Gln Leu Asp Ala Ile Thr Lys Gln Leu 545 550 555 560 GlnArg Asp Glu Asn Glu Ala Gln Ser Leu Arg Gln Asp Glu Gln Ala 565 570 575Leu Thr Gln Gln Trp Gln Ala Val Thr Ala Ser Leu Asn Ile Thr Leu 580 585590 Gln Pro Leu Asp Asp Ile Gln Pro Trp Leu Asp Ala Gln Asp Glu His 595600 605 Glu Arg Gln Leu Arg Leu Leu Ser Gln Arg His Glu Leu Gln Gly Gln610 615 620 Ile Ala Ala His Asn Gln Gln Ile Ile Gln Tyr Gln Gln Gln IleGlu 625 630 635 640 Gln Arg Gln Gln Leu Leu Leu Thr Thr Leu Thr Gly TyrAla Leu Thr 645 650 655 Leu Pro Gln Glu Asp Glu Glu Glu Ser Trp Leu AlaThr Arg Gln Gln 660 665 670 Glu Ala Gln Ser Trp Gln Gln Arg Gln Asn GluLeu Thr Ala Leu Gln 675 680 685 Asn Arg Ile Gln Gln Leu Thr Pro Ile LeuGlu Thr Leu Pro Gln Ser 690 695 700 Asp Glu Leu Pro His Cys Glu Glu ThrVal Val Leu Glu Asn Trp Arg 705 710 715 720 Gln Val His Glu Gln Cys LeuAla Leu His Ser Gln Gln Gln Thr Leu 725 730 735 Gln Gln Gln Asp Val LeuAla Ala Gln Ser Leu Gln Lys Ala Gln Ala 740 745 750 Gln Phe Asp Thr AlaLeu Gln Ala Ser Val Phe Asp Asp Gln Gln Ala 755 760 765 Phe Leu Ala AlaLeu Met Asp Glu Gln Thr Leu Thr Gln Leu Glu Gln 770 775 780 Leu Lys GlnAsn Leu Glu Asn Gln Arg Arg Gln Ala Gln Thr Leu Val 785 790 795 800 ThrGln Thr Ala Glu Thr Leu Ala Gln His Gln Gln His Arg Pro Asp 805 810 815Asp Gly Leu Ala Leu Thr Val Thr Val Glu Gln Ile Gln Gln Glu Leu 820 825830 Ala Gln Thr His Gln Lys Leu Arg Glu Asn Thr Thr Ser Gln Gly Glu 835840 845 Ile Arg Gln Gln Leu Lys Gln Asp Ala Asp Asn Arg Gln Gln Gln Gln850 855 860 Thr Leu Met Gln Gln Ile Ala Gln Met Thr Gln Gln Val Glu AspTrp 865 870 875 880 Gly Tyr Leu Asn Ser Leu Ile Gly Ser Lys Glu Gly AspLys Phe Arg 885 890 895 Lys Phe Ala Gln Gly Leu Thr Leu Asp Asn Leu ValHis Leu Ala Asn 900 905 910 Gln Gln Leu Thr Arg Leu His Gly Arg Tyr LeuLeu Gln Arg Lys Ala 915 920 925 Ser Glu Ala Leu Glu Val Glu Val Val AspThr Trp Gln Ala Asp Ala 930 935 940 Val Arg Asp Thr Arg Thr Leu Ser GlyGly Glu Ser Phe Leu Val Ser 945 950 955 960 Leu Ala Leu Ala Leu Ala LeuSer Asp Leu Val Ser His Lys Thr Arg 965 970 975 Ile Asp Ser Leu Phe LeuAsp Glu Gly Phe Gly Thr Leu Asp Ser Glu 980 985 990 Thr Leu Asp Thr AlaLeu Asp Ala Leu Asp Ala Leu Asn Ala Ser Gly 995 1000 1005 Lys Thr IleGly Val Ile Ser His Val Glu Ala Met Lys Glu Arg Ile 1010 1015 1020 ProVal Gln Ile Lys Val Lys Lys Ile Asn Gly Leu Gly Tyr Ser Lys 1025 10301035 1040 Leu Glu Ser Thr Phe Ala Val Lys 1045 1239 base pairs nucleicacid single linear other nucleic acid /desc = ”DNA (genomic) (p14b74)“CDS 1..1236 86 ATG ATG AAA GAA AAA GTG ATT TTT CTC GTT GAC ATG CAA TCGTTT TAT 48 Met Met Lys Glu Lys Val Ile Phe Leu Val Asp Met Gln Ser PheTyr 1 5 10 15 GCA TCT GTA GAG AAA GCG GAA AAT CCA CAT TTG AAA AAT AGGCCC GTC 96 Ala Ser Val Glu Lys Ala Glu Asn Pro His Leu Lys Asn Arg ProVal 20 25 30 ATT GTT TCG GGT GAC CCT GAA AAA AGG GGC GGA GTC GTA TTG GCTGCC 144 Ile Val Ser Gly Asp Pro Glu Lys Arg Gly Gly Val Val Leu Ala Ala35 40 45 TGC CCG CTG GCG AAA CAA AAG GGT GTG GTG AAT GCT TCA CGG CTG TGG192 Cys Pro Leu Ala Lys Gln Lys Gly Val Val Asn Ala Ser Arg Leu Trp 5055 60 GAG GCG CAG GAA AAG TGT CCT GAG GCT GTT GTG CTC CGG CCG CGT ATG240 Glu Ala Gln Glu Lys Cys Pro Glu Ala Val Val Leu Arg Pro Arg Met 6570 75 80 CAG CGG TAT ATT GAT GTA TCA CTG CAA ATT ACG GCC ATT CTC GAG GAG288 Gln Arg Tyr Ile Asp Val Ser Leu Gln Ile Thr Ala Ile Leu Glu Glu 8590 95 TAT ACA GAC CTT GTG GAG CCG TAT TCC ATC GAT GAA CAG TTC ATG GAC336 Tyr Thr Asp Leu Val Glu Pro Tyr Ser Ile Asp Glu Gln Phe Met Asp 100105 110 ATT ACA GGC AGC CAG AAG CTG TTT GGG ACG CCG ATG GAG ATC GCG AAA384 Ile Thr Gly Ser Gln Lys Leu Phe Gly Thr Pro Met Glu Ile Ala Lys 115120 125 AGC ATT CAG GGC AGA ATC ATG CGG GAG ATC GGC GTT TAT GCA CGG GTC432 Ser Ile Gln Gly Arg Ile Met Arg Glu Ile Gly Val Tyr Ala Arg Val 130135 140 GGA ATC GGC CCT AAC AAA GCG CTG GCC AAA ATT GCG TGT GAC AAT TTT480 Gly Ile Gly Pro Asn Lys Ala Leu Ala Lys Ile Ala Cys Asp Asn Phe 145150 155 160 GCC AAA AAG AAT AAG AAC GGT ATT TTT ACC TTA ACG AAA GAA AATATG 528 Ala Lys Lys Asn Lys Asn Gly Ile Phe Thr Leu Thr Lys Glu Asn Met165 170 175 AAA ACC GAA ATG TGG CCG CTC CCG GTG GGC AGC ATG TTT GGC GTCGGG 576 Lys Thr Glu Met Trp Pro Leu Pro Val Gly Ser Met Phe Gly Val Gly180 185 190 AGC CGC ATG AAG CAT CAT TTA AAT CGA ATG GGC ATC AGC ACG ATCGGC 624 Ser Arg Met Lys His His Leu Asn Arg Met Gly Ile Ser Thr Ile Gly195 200 205 GGG CTC GCG GCT TTT CCG CTC GAT CTT TTA AAA AAG AAA TGG GGCATT 672 Gly Leu Ala Ala Phe Pro Leu Asp Leu Leu Lys Lys Lys Trp Gly Ile210 215 220 AAC GGC CAC GTG CTG TGG ATG ACG GCA AAC GGA ATC GAC TAT TCCCCT 720 Asn Gly His Val Leu Trp Met Thr Ala Asn Gly Ile Asp Tyr Ser Pro225 230 235 240 GTG TCA ACT TCG TCT CTG GAC GGG CAA AAG GCG ATA GGT CATGGA ATG 768 Val Ser Thr Ser Ser Leu Asp Gly Gln Lys Ala Ile Gly His GlyMet 245 250 255 ACT CTC CCG AGA GAC TAC GAA CAC TTT GAC AAA GAA ATC AAGGTC GTA 816 Thr Leu Pro Arg Asp Tyr Glu His Phe Asp Lys Glu Ile Lys ValVal 260 265 270 TTG CTT GAG CTG AGT GAA GAG GTG TGC AGG CGA AGC CGA AACGCC GGG 864 Leu Leu Glu Leu Ser Glu Glu Val Cys Arg Arg Ser Arg Asn AlaGly 275 280 285 GTC ATG GGG CAG ACA GTG TCA GTG AGC TGC CGG GGT GCT GATTTT GAT 912 Val Met Gly Gln Thr Val Ser Val Ser Cys Arg Gly Ala Asp PheAsp 290 295 300 TGG CCG ACG GGC TTC AAC CGG CAA GTG AAG CTG GCA GAG CCGACT AAT 960 Trp Pro Thr Gly Phe Asn Arg Gln Val Lys Leu Ala Glu Pro ThrAsn 305 310 315 320 TCT ACG CAG GAT GTA TAT GAG GCT GTA CGA CGG CTG TTTCTT ACA TTT 1008 Ser Thr Gln Asp Val Tyr Glu Ala Val Arg Arg Leu Phe LeuThr Phe 325 330 335 TGG GAC GGG AAA CCC GTC CGC CGC CTC GGT GTC AAT CTGTCT CAG CTC 1056 Trp Asp Gly Lys Pro Val Arg Arg Leu Gly Val Asn Leu SerGln Leu 340 345 350 TCA TCT GAT GAC ATA TGG CAG CTC AAT TTA TTT CAG GATTAT GCA AAG 1104 Ser Ser Asp Asp Ile Trp Gln Leu Asn Leu Phe Gln Asp TyrAla Lys 355 360 365 AAA ATG AGC CTA GGC TAT GTG ATG GAT GGC ATT AAA AATCGA TTC GGC 1152 Lys Met Ser Leu Gly Tyr Val Met Asp Gly Ile Lys Asn ArgPhe Gly 370 375 380 GAT ACA GCA ATC ATC AGG GCG GCG TCA CTG ACA GCG GCAGGC CAG GCA 1200 Asp Thr Ala Ile Ile Arg Ala Ala Ser Leu Thr Ala Ala GlyGln Ala 385 390 395 400 TTT GAA CGT GCG GCT AAA ATA GGG GGG CAT TAT AAATGA 1239 Phe Glu Arg Ala Ala Lys Ile Gly Gly His Tyr Lys 405 410 412amino acids amino acid linear protein 87 Met Met Lys Glu Lys Val Ile PheLeu Val Asp Met Gln Ser Phe Tyr 1 5 10 15 Ala Ser Val Glu Lys Ala GluAsn Pro His Leu Lys Asn Arg Pro Val 20 25 30 Ile Val Ser Gly Asp Pro GluLys Arg Gly Gly Val Val Leu Ala Ala 35 40 45 Cys Pro Leu Ala Lys Gln LysGly Val Val Asn Ala Ser Arg Leu Trp 50 55 60 Glu Ala Gln Glu Lys Cys ProGlu Ala Val Val Leu Arg Pro Arg Met 65 70 75 80 Gln Arg Tyr Ile Asp ValSer Leu Gln Ile Thr Ala Ile Leu Glu Glu 85 90 95 Tyr Thr Asp Leu Val GluPro Tyr Ser Ile Asp Glu Gln Phe Met Asp 100 105 110 Ile Thr Gly Ser GlnLys Leu Phe Gly Thr Pro Met Glu Ile Ala Lys 115 120 125 Ser Ile Gln GlyArg Ile Met Arg Glu Ile Gly Val Tyr Ala Arg Val 130 135 140 Gly Ile GlyPro Asn Lys Ala Leu Ala Lys Ile Ala Cys Asp Asn Phe 145 150 155 160 AlaLys Lys Asn Lys Asn Gly Ile Phe Thr Leu Thr Lys Glu Asn Met 165 170 175Lys Thr Glu Met Trp Pro Leu Pro Val Gly Ser Met Phe Gly Val Gly 180 185190 Ser Arg Met Lys His His Leu Asn Arg Met Gly Ile Ser Thr Ile Gly 195200 205 Gly Leu Ala Ala Phe Pro Leu Asp Leu Leu Lys Lys Lys Trp Gly Ile210 215 220 Asn Gly His Val Leu Trp Met Thr Ala Asn Gly Ile Asp Tyr SerPro 225 230 235 240 Val Ser Thr Ser Ser Leu Asp Gly Gln Lys Ala Ile GlyHis Gly Met 245 250 255 Thr Leu Pro Arg Asp Tyr Glu His Phe Asp Lys GluIle Lys Val Val 260 265 270 Leu Leu Glu Leu Ser Glu Glu Val Cys Arg ArgSer Arg Asn Ala Gly 275 280 285 Val Met Gly Gln Thr Val Ser Val Ser CysArg Gly Ala Asp Phe Asp 290 295 300 Trp Pro Thr Gly Phe Asn Arg Gln ValLys Leu Ala Glu Pro Thr Asn 305 310 315 320 Ser Thr Gln Asp Val Tyr GluAla Val Arg Arg Leu Phe Leu Thr Phe 325 330 335 Trp Asp Gly Lys Pro ValArg Arg Leu Gly Val Asn Leu Ser Gln Leu 340 345 350 Ser Ser Asp Asp IleTrp Gln Leu Asn Leu Phe Gln Asp Tyr Ala Lys 355 360 365 Lys Met Ser LeuGly Tyr Val Met Asp Gly Ile Lys Asn Arg Phe Gly 370 375 380 Asp Thr AlaIle Ile Arg Ala Ala Ser Leu Thr Ala Ala Gly Gln Ala 385 390 395 400 PheGlu Arg Ala Ala Lys Ile Gly Gly His Tyr Lys 405 410 441 base pairsnucleic acid single linear other nucleic acid /desc = ”DNA (genomic)(p14c13)“ 88 TAAGGGGACT TATATAACTG TATGTTTGTG TAGTGTTTAT GTCAGTAAGCTAAATTTACA 60 TTCATGTTAT GTYTCATTAA ACCAATTACT CACGTTTTGG TGCATATTAAATCTTTTATA 120 TCGATCATAC ATCTATCATC ATTTTTATTT CTAAAATACA AACTGAATACTTTGCTAGAA 180 TTTGTTACAG CAATCATTGC TCATGCATTT TATAAATTAC AATTAGACAAATATGACATT 240 TGATATCACA CACTTCAAAC ACACACATAT ATAATCAGAC ATAAATTGTTATGCTAAGGG 300 TTTATTCACC AAAAATATAA TACATATGGG CTTGTTTTGA GTCCATATTGAATGAATTAA 360 AAAGTATACT CCACTCAATC ATTTACAAAT AGGTGGTGCC ACTCCNAATTTATTTTATGG 420 CCAACCCCAA ATATGAGAAC A 441 909 base pairs nucleic acidsingle linear other nucleic acid /desc = ”DNA (genomic) (p15b9)“ CDS1..906 89 ATG AAC CAA AAA GGC AGA GGG CTT GAG ATC CTC ATC AAT GAA AAACAG 48 Met Asn Gln Lys Gly Arg Gly Leu Glu Ile Leu Ile Asn Glu Lys Gln 15 10 15 GAC GGC CAA TGG CTG TTT TCC GTA CTC AAA ACA GCG CTC AAA GCT TCT96 Asp Gly Gln Trp Leu Phe Ser Val Leu Lys Thr Ala Leu Lys Ala Ser 20 2530 AAA CCA GTG ATA CAA GAC TGG ATG TCC CAT CAA CAG ATA AAG GTC AAT 144Lys Pro Val Ile Gln Asp Trp Met Ser His Gln Gln Ile Lys Val Asn 35 40 45CAC GAA TCC GTC TTA AAC AAT ATG ATT GTA AAA AAG GGA GAC CGC GTG 192 HisGlu Ser Val Leu Asn Asn Met Ile Val Lys Lys Gly Asp Arg Val 50 55 60 TTCATT GAT CTT CAG GAA AGT GAA GCA TCT TCG GTC ATT CCG GAG TAT 240 Phe IleAsp Leu Gln Glu Ser Glu Ala Ser Ser Val Ile Pro Glu Tyr 65 70 75 80 GGCGAG CTT GAT ATT TTA TTT GAG GAC AAT CAT ATG CTC ATC ATC AAT 288 Gly GluLeu Asp Ile Leu Phe Glu Asp Asn His Met Leu Ile Ile Asn 85 90 95 AAA CCCGCT GGC ATC GCG ACG CAT CCG AAT GAG GAT GGG CAA ACC GGC 336 Lys Pro AlaGly Ile Ala Thr His Pro Asn Glu Asp Gly Gln Thr Gly 100 105 110 ACA CTGGCT AAT TTG ATC GCG TAT CAT TAT CAG ATA AAT GGC GAA ACA 384 Thr Leu AlaAsn Leu Ile Ala Tyr His Tyr Gln Ile Asn Gly Glu Thr 115 120 125 TGT AAGGTG CGC CAC GTC CAT CGT CTT GAT CAG GAT ACA TCT GGC GCT 432 Cys Lys ValArg His Val His Arg Leu Asp Gln Asp Thr Ser Gly Ala 130 135 140 ATC GTTTTT GCC AAG CAT CGT TTG GCA CAC GCC ATC TTA GAC CAG CAG 480 Ile Val PheAla Lys His Arg Leu Ala His Ala Ile Leu Asp Gln Gln 145 150 155 160 TTAGAG AAA AAG ACG CTG AAG CGT ACG TAT ACC GCT ATC GCT GAA GGT 528 Leu GluLys Lys Thr Leu Lys Arg Thr Tyr Thr Ala Ile Ala Glu Gly 165 170 175 AAGCTA CGG ACG AAA AAA GGG ACA ATT AAT CCA CCG ATC GGC AGA GAC 576 Lys LeuArg Thr Lys Lys Gly Thr Ile Asn Pro Pro Ile Gly Arg Asp 180 185 190 CGCTCA CAC CCG ACA AGA CGC CGG GTT TCA CCA GGC GGG CAA ACA GCC 624 Arg SerHis Pro Thr Arg Arg Arg Val Ser Pro Gly Gly Gln Thr Ala 195 200 205 GTCACT CAT TTC AAG GTA ATG GCC AGC AAT GCG AAA GAA CGG CTG TCG 672 Val ThrHis Phe Lys Val Met Ala Ser Asn Ala Lys Glu Arg Leu Ser 210 215 220 CTCGTT GAA TTA GAG CTG GAA ACA GGC AGA ACA CAC CAA ATC CGT GTT 720 Leu ValGlu Leu Glu Leu Glu Thr Gly Arg Thr His Gln Ile Arg Val 225 230 235 240CAT CTG GCG AGC CTC GGC CAT CCG TTG ACA GGA GAC TCG CTT TAC GGA 768 HisLeu Ala Ser Leu Gly His Pro Leu Thr Gly Asp Ser Leu Tyr Gly 245 250 255GGC GGG AGC AAG CTG CTA AAC AGG CAG GCA CTG CAC GCC AAT AAA GTA 816 GlyGly Ser Lys Leu Leu Asn Arg Gln Ala Leu His Ala Asn Lys Val 260 265 270CAA GCG GTT CAC CCG ATA ACA GAC GAG CTC ATA GTT GCT GAA GCC CCT 864 GlnAla Val His Pro Ile Thr Asp Glu Leu Ile Val Ala Glu Ala Pro 275 280 285TTC CCT GCT GAT ATG AAA AAC CTT TGC CGC ACA TAT TTT TCA 906 Phe Pro AlaAsp Met Lys Asn Leu Cys Arg Thr Tyr Phe Ser 290 295 300 TGA 909 302amino acids amino acid linear protein 90 Met Asn Gln Lys Gly Arg Gly LeuGlu Ile Leu Ile Asn Glu Lys Gln 1 5 10 15 Asp Gly Gln Trp Leu Phe SerVal Leu Lys Thr Ala Leu Lys Ala Ser 20 25 30 Lys Pro Val Ile Gln Asp TrpMet Ser His Gln Gln Ile Lys Val Asn 35 40 45 His Glu Ser Val Leu Asn AsnMet Ile Val Lys Lys Gly Asp Arg Val 50 55 60 Phe Ile Asp Leu Gln Glu SerGlu Ala Ser Ser Val Ile Pro Glu Tyr 65 70 75 80 Gly Glu Leu Asp Ile LeuPhe Glu Asp Asn His Met Leu Ile Ile Asn 85 90 95 Lys Pro Ala Gly Ile AlaThr His Pro Asn Glu Asp Gly Gln Thr Gly 100 105 110 Thr Leu Ala Asn LeuIle Ala Tyr His Tyr Gln Ile Asn Gly Glu Thr 115 120 125 Cys Lys Val ArgHis Val His Arg Leu Asp Gln Asp Thr Ser Gly Ala 130 135 140 Ile Val PheAla Lys His Arg Leu Ala His Ala Ile Leu Asp Gln Gln 145 150 155 160 LeuGlu Lys Lys Thr Leu Lys Arg Thr Tyr Thr Ala Ile Ala Glu Gly 165 170 175Lys Leu Arg Thr Lys Lys Gly Thr Ile Asn Pro Pro Ile Gly Arg Asp 180 185190 Arg Ser His Pro Thr Arg Arg Arg Val Ser Pro Gly Gly Gln Thr Ala 195200 205 Val Thr His Phe Lys Val Met Ala Ser Asn Ala Lys Glu Arg Leu Ser210 215 220 Leu Val Glu Leu Glu Leu Glu Thr Gly Arg Thr His Gln Ile ArgVal 225 230 235 240 His Leu Ala Ser Leu Gly His Pro Leu Thr Gly Asp SerLeu Tyr Gly 245 250 255 Gly Gly Ser Lys Leu Leu Asn Arg Gln Ala Leu HisAla Asn Lys Val 260 265 270 Gln Ala Val His Pro Ile Thr Asp Glu Leu IleVal Ala Glu Ala Pro 275 280 285 Phe Pro Ala Asp Met Lys Asn Leu Cys ArgThr Tyr Phe Ser 290 295 300 471 base pairs nucleic acid single linearother nucleic acid /desc = ”DNA (genomic) (p15b32)“ CDS 1..468 91 ATGGAT GAG TTG CAG CAG GTC TCC GAT GGC TGG CTT GCG CAC CAC AAT 48 Met AspGlu Leu Gln Gln Val Ser Asp Gly Trp Leu Ala His His Asn 1 5 10 15 ACGCGG GAA AAG CGC TTC TCG CTT GGC GCT TTC GAA CCG GAC TAT ATT 96 Thr ArgGlu Lys Arg Phe Ser Leu Gly Ala Phe Glu Pro Asp Tyr Ile 20 25 30 CTG TCGCAA CCC GTC GCC GTG CTG CGC AAG GAT GGA AAA ATC ACC GCC 144 Leu Ser GlnPro Val Ala Val Leu Arg Lys Asp Gly Lys Ile Thr Ala 35 40 45 TTC GCC AATCTG ATG GTG ACG GAG ACG AAA AAG GAA GCC ACC ATC GAC 192 Phe Ala Asn LeuMet Val Thr Glu Thr Lys Lys Glu Ala Thr Ile Asp 50 55 60 CTC ATG CGC TTTTCG GCG GAT GCG CGC GCG GCT CGA TGG ATT TCC TCT 240 Leu Met Arg Phe SerAla Asp Ala Arg Ala Ala Arg Trp Ile Ser Ser 65 70 75 80 TCG TCA GCA TCATGC AGC ATC TGC GCG AGG CGG GAT ATG AAA GCT TCA 288 Ser Ser Ala Ser CysSer Ile Cys Ala Arg Arg Asp Met Lys Ala Ser 85 90 95 ATC TCG GCA TGG CGCCCA TGT CCG GCA TGT CGA AGC GCG ATG CCG CGC 336 Ile Ser Ala Trp Arg ProCys Pro Ala Cys Arg Ser Ala Met Pro Arg 100 105 110 CGG TCT GGG ACC GTATCG GCA GCA CGC TGT TCG AGC ACG GCG AAC GTT 384 Arg Ser Gly Thr Val SerAla Ala Arg Cys Ser Ser Thr Ala Asn Val 115 120 125 TCT ACA ACT TCA AGGGAC TTC GCG CCT TCA AGG CAA AGT TCC ACC CGA 432 Ser Thr Thr Ser Arg AspPhe Ala Pro Ser Arg Gln Ser Ser Thr Arg 130 135 140 AAT GGG AAC CCC GTTACC TTG CTG TGC AGA ACG GCG TGA 471 Asn Gly Asn Pro Val Thr Leu Leu CysArg Thr Ala 145 150 155 156 amino acids amino acid linear protein 92 MetAsp Glu Leu Gln Gln Val Ser Asp Gly Trp Leu Ala His His Asn 1 5 10 15Thr Arg Glu Lys Arg Phe Ser Leu Gly Ala Phe Glu Pro Asp Tyr Ile 20 25 30Leu Ser Gln Pro Val Ala Val Leu Arg Lys Asp Gly Lys Ile Thr Ala 35 40 45Phe Ala Asn Leu Met Val Thr Glu Thr Lys Lys Glu Ala Thr Ile Asp 50 55 60Leu Met Arg Phe Ser Ala Asp Ala Arg Ala Ala Arg Trp Ile Ser Ser 65 70 7580 Ser Ser Ala Ser Cys Ser Ile Cys Ala Arg Arg Asp Met Lys Ala Ser 85 9095 Ile Ser Ala Trp Arg Pro Cys Pro Ala Cys Arg Ser Ala Met Pro Arg 100105 110 Arg Ser Gly Thr Val Ser Ala Ala Arg Cys Ser Ser Thr Ala Asn Val115 120 125 Ser Thr Thr Ser Arg Asp Phe Ala Pro Ser Arg Gln Ser Ser ThrArg 130 135 140 Asn Gly Asn Pro Val Thr Leu Leu Cys Arg Thr Ala 145 150155 192 base pairs nucleic acid single linear other nucleic acid /desc =”DNA (genomic) (p15c4)“ CDS 1..189 93 ATG CCT ATT GCC CAG ATC CAC ATCCTT GAA GGC CGC AGC GAC GAG CAG 48 Met Pro Ile Ala Gln Ile His Ile LeuGlu Gly Arg Ser Asp Glu Gln 1 5 10 15 AAG GAA ACC CTG ATT CGG GAA GTCAGT GAG GCC ATC TCG CGC TCC CTG 96 Lys Glu Thr Leu Ile Arg Glu Val SerGlu Ala Ile Ser Arg Ser Leu 20 25 30 GAT GCG CCG CTG ACC AGC GTG CGA GTGATT ATC ACG GAG ATG GCC AAG 144 Asp Ala Pro Leu Thr Ser Val Arg Val IleIle Thr Glu Met Ala Lys 35 40 45 GGC CAC TTC GGC ATC GGC GGC GAA CTG GCCAGC AAG GTC AGA CGC 189 Gly His Phe Gly Ile Gly Gly Glu Leu Ala Ser LysVal Arg Arg 50 55 60 TGA 192 63 amino acids amino acid linear protein 94Met Pro Ile Ala Gln Ile His Ile Leu Glu Gly Arg Ser Asp Glu Gln 1 5 1015 Lys Glu Thr Leu Ile Arg Glu Val Ser Glu Ala Ile Ser Arg Ser Leu 20 2530 Asp Ala Pro Leu Thr Ser Val Arg Val Ile Ile Thr Glu Met Ala Lys 35 4045 Gly His Phe Gly Ile Gly Gly Glu Leu Ala Ser Lys Val Arg Arg 50 55 6030 base pairs nucleic acid single linear other nucleic acid /desc =”primer“ 95 GGAGCTCACT AGTCGGAGGC ATCAGTGACC 30 27 base pairs nucleicacid single linear other nucleic acid /desc = ”primer“ 96 GGGATCCCATGAGAATTCTT GAAGACG 27 89 base pairs nucleic acid single linear othernucleic acid /desc = ”oligonucleotide“ 97 CTAGGTACCT ACAACCTCAAGCTTNKNKNK NKNKNKNKNK NKNKNKNKNK NKNKNKNKNK 60 NKNKAAGCTT GGTTAGAATGGGTACCATG 89 24 base pairs nucleic acid single linear other nucleic acid/desc = ”primer“ 98 CTAGAATTCT ACAACCTCAA GCTT 24 25 base pairs nucleicacid single linear other nucleic acid /desc = ”primer“ 99 AAGCTTGGTTAGAATGGAAT TCATG 25 15 base pairs nucleic acid single linear othernucleic acid /desc = ”primer“ 100 GAATTCCATT CTAAC 15 18 base pairsnucleic acid single linear other nucleic acid /desc = ”primer“ 101ATTCCATTCT AACCAAGC 18 21 base pairs nucleic acid single linear othernucleic acid /desc = ”primer“ 102 TGAACTGCCA CTGTAGAGAG A 21 40 basepairs nucleic acid single linear other nucleic acid /desc = ”primer“ 103GGCCATCGAT AATGAAATTA ATTAACGAGA GACAAATAGG 40 55 base pairs nucleicacid single linear other nucleic acid /desc = ”primer“ 104 GGCCGGATCCCTAGTGATGG TGATGGTGAT GAAAAATTCT GTCTTTAACT TTTTT 55 36 base pairsnucleic acid single linear other nucleic acid /desc = ”primer“ 105GGCCGGTACC AAATTAATTA ACGAGAGACA AATAGG 36 55 base pairs nucleic acidsingle linear other nucleic acid /desc = ”primer“ 106 GGCCGGATCCCTAGTGATGG TGATGGTGAT GAAAAATTCT GTCTTTAACT TTTTT 55

What is claimed is:
 1. A method of identifying an anti-bacterial agentcomprising the steps of assaying potential agents for the ability tointerfere with expression of virulence gene products represented by theDNA sequences set forth in any one of SEQ ID NOS: 1 through 94, andidentifying an agent that interferes with expression of said virulencegene products.
 2. A method of identifying an anti-bacterial agentcomprising the steps of assaying potential agents for the ability tointerfere with the function of a bacterial protein encoded in whole orin part by a DNA sequence set forth in any one of SEQ ID NOS: 1 through94 or the complementary strand thereof, and identifying an agent thatinterferes with the function of said bacterial protein.
 3. The method ofclaim 2 wherein the protein is involved in cell surface metabolism andis encoded by the DNA sequence of any one of SEQ ID NOS: 1, 6, 60 and 65(corresponding to signature tag identification numbers P2C73, P11C29,P13C83, P9B65 and P10B89).
 4. The method of claim 2 wherein the proteinis an enzyme involved in a cellular biosynthetic pathway and is encodedby the DNA sequence of any one of SEQ ID NOS: 4, 11, 13, 22, 25, 27, 29,31, 33, 35 and 82 (corresponding to signature tag identification numbersP9B74, P5C4, P9B66, P14C15, P13B26, P7C18, P15C31, P10B18, P6B18,P10B66, P10C34 and P12C3).
 5. The method of claim 2 wherein the proteinis a component of the TCA cycle and is encoded by the DNA sequence ofany one of SEQ ID NOS: 37, 39, 41, 43 and 44 (corresponding to signaturetag identification numbers P4C27, P4C52, P10B2, P10C20 and P12C32). 6.The method of claim 2 wherein the protein is an oligopeptide transportproteins and is encoded by the DNA sequence of any one of SEQ ID NOS:15, 17, 19, 54, 75, 77 and 79 (corresponding to signature tagidentification numbers P7C26, P10C15, P5C3, P11C66 and P5C34).
 7. Themethod of claim 2 wherein the protein is involved in cellular regulatoryand repair processes and is encoded by the DNA sequence of any one ofSEQ ID NOS: 8, 21, 46 and 84 (corresponding to signature tagidentification numbers P4C15, P6C63, P13B74, P13C72, P10B30 and P14B25).8. The method of any one of claims 1 through 7 wherein the assay used isan enzyme assay.
 9. The method of any one of claims 1 through 7 whereinthe assay used is a binding assay.
 10. The method of any one of claims 1through 7 wherein the assay used is a two-hybrid screening assay. 11.The method of any one of claims 1 through 7 wherein the assay is beingused to screen for a ligand by binding to target protein.
 12. The methodof any one of claims 1 through 7 wherein the assay used is an affinityultrafiltration assay.
 13. A Staphylococcus aureus organism containing afunctional mutation in a gene represented by any one of SEQ ID NOS: 1through 94, said functional mutation resulting in a reduction invirulence of the organism.
 14. A vaccine composition comprising the S.aureus organism of claim 13.